Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	863,370	G→A	12.1%	A168A (GCG→GCA)	hemH →	ferrochelatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	863,370	0	G	A	12.1%	79.4 / 3.5	33	A168A (GCG→GCA)	hemH	ferrochelatase
Reads supporting (aligned to +/- strand):  ref base G (16/13);  new base A (2/2);  total (18/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.64e-01

TTGGCACCGCTTTATCCACAGTTCGCCGACAGCACGGTGACCACTGTGATCGAGCAGGCAAAACAGACGGTCAGCGAGCACCAGTTACCGTTGCAGATGCGCGTGCTGCAGCCGTTCTATGAGCACCCGGCGTACATCGAGGCGCTGGCAGCCAGCGCCCGGCCGTACCTGGAGCAGGGTTACGACCACCTGCTACTGAGCTTCCATGGTTTGCCGGAACGGCACCTGAAGAAACTGTTTCCGAAGGGGGTCAAGCACGACCTGCGCGCTGC  >  NC_002947/863227‑863498                                                                                                                                                |                                                                                                                                 ttGGCACCGCTTTATCCACAGTTCGCCGACAGCACGGTGACCACTGTGATCGAGCAGGCAAAACAGACGGTCAGCGAGCACCAGTTACCGTTGCAGATGCGCGTGCTGCAGCCGTTCTATGAGCACCCGGCGTACATCGCGGCGCTGGc                                                                                                                             >  7:237321/1‑149 (MQ=255)   ggCACCGCTTTATCCACAGTTCGCCGACAGCACGGTGACCACTGTGATCGAGCAGGCAAAACAGACGGTCAGCGAGCACCAGTTACCGTTGCAGATGCGCGTGCTGCAGCCGTTCTATGAGCACCCGGCGTACATCGAGGCGCTGGca                                                                                                                            <  2:309525/148‑1 (MQ=255)    gCACCGCTTTATCCACAGTTCGCCGACAGCACGGTGACCACTGTGATCGAGCAGGCAAAACAGACGGTCAGCGAGCACCAGTTACCGTTGCAGATGCGCGTGCTGCAGCCGTTCTATGAGCACCCGGCGTACATCGAGGCGCTGGcagc                                                                                                                          >  1:67252/1‑149 (MQ=255)        cGCTTTATCCACAGTTCGCCGACAGCACGGTGACCACTGTGATCGAGCAGGCAAAACAGACGGTCAGCGAGCACCAGTTACCGTTGCAGATGCGCGTGCTGCAGCCGTTCTATGAGCACCCGGCGTACATCGAGGCGCTGGcagccagc                                                                                                                      >  8:50301/1‑149 (MQ=255)          cTTTATCCACAGTTCGCCGACAGCACGGTGACCACTGTGATCGAGCAGGCAAAACAGACGGTCAGCGAGCACCAGTTACCGTTGCAGATGCGCGTGCTGCAGCCGTTCTATGAGCACCCGGCGTACATCGAGGCGCTGGCAGCCAgcgc                                                                                                                    <  5:90045/149‑1 (MQ=255)                 cacaGTTCGCCGACAGCACGGTGACCACTGTGATCGAGCAGGCAAAACAGACGGTCAGCGAGCACCAGTTACCGTTGCAGATGCGCGTGCTGCAGCCGTTCTATGAGCACCCGGCGTACATCGAGGCGCTGGCAGCCAGCGCCCGGCCg                                                                                                             >  4:297506/1‑149 (MQ=255)                  acaGTTCGCCGACAGCACGGTGACCACTGTGATCGAGCAGGCAAAACAGACGGTCAGCGAGCACCAGTTACCGTTGCAGATGCGCGTGCTGCAGCCGTTCTATGAGCACCCGGCGTACATCGAGGCGCTGGCAGCCAGCGCCCGGCCg                                                                                                             >  7:349920/1‑148 (MQ=255)                     gTTCGCCGACAGCACGGTGACCACTGTGATCGAGCAGGCAAAACAGACGGTCAGCGAGCACCAGTTACCGTTGCAGATGCGCGTGCTGCAGCCGTTCTATGAGCACCCGGCGTACATCGAGGCGCTGGCAGCCAGCGCCCGGCCGTAcg                                                                                                         >  8:180158/1‑148 (MQ=255)                      ttCGCCGACAGCACGGTGACCACTGTGATCGAGCAGGCAAAACAGACGGTCAGCGAGCACCAGTTACCGTTGCAGATGCGCGTGCTGCAGCCGTTCTATGAGCACCCGGCGTACATCGAGGCGCTGGCAGCCAGCGCCCGGCCGTACCt                                                                                                        >  1:285916/1‑149 (MQ=255)                        cGCCGACAGCACGGTGACCACTGTGATCGAGCAGGCAAAACAGACGGTCAGCGAGCACCAGTTACCGTTGCAGATGCGCGTGCTGCAGCCGTTCTATGAGCACCCGGCGTACATCGAGGCGCTGGCAGCCAGCGCCCGGCCGTACCTg                                                                                                       >  5:91374/1‑148 (MQ=255)                             aCAGCACGGTGACCACTGTGATCGAGCAGGCAAAACAGACGGTCAGCGAGCACCAGTTACCGTTGCAGATGCGCGTGCTGCAGCCGTTCTATGAGCACCCGGCGTACATCGAGGCGCTGGCAGCCAGCGCCCGGCCGTACCTGGAGCAg                                                                                                 <  8:231433/149‑1 (MQ=255)                                 cACGGTGACCACTGTGATCGAGCAGGCAAAACAGACGGTCAGCGAGCACCAGTTACCATTGCAGATGCGCGTGCTGCAGCCGTTCTATGAGCACCCGGCGTACATCGTGGCGCTGGCAGCCAGCGCCCGGCCGTACCTGGAGCAGGGtt                                                                                             <  6:155385/149‑1 (MQ=255)                                  aCGGTGACCACTGTGATCGAGCAGGCAAAACAGACGGTCAGCGAGCACCAGTTACCGTTGCAGATGCGCGTGCTGCAGCCGTTCTATGAGCACCCGGCGTACATCGAGGCGCTGGCAGCCAGCGCCCGGCCGTACCTGGAGCAGGGTTa                                                                                            <  6:91374/149‑1 (MQ=255)                                    ggTGACCACTGTGATCGAGCAGGCAAAACAGACGGTCAGCGAGCACCAGTTACCGTTGCAGATGCGCGTGCTGCAGCCGTTCTATGAGCACCCGGCGTACATCGAGGCGCTGGCAGCCAGCGCCCGGCCGTACCTGGAGCAGGGTTAc                                                                                           >  4:138231/1‑148 (MQ=255)                                         ccACTGTGATCGAGCAGGAAAAACAGACGGTCAGCGCGCACCAGTTCCCGTTGCAGATGCGCGTGCTGCAGCCGTTCTATGAGCACCCGGCGTACATCGAGGCGCTGGCAGCCAGCGCCCGGCCGTACCTGGAGCAGGGTTACGaccac                                                                                     <  2:67252/149‑1 (MQ=255)                                                          gCAAAACAGACGGTCAGCGAGCACCAGTTACCGTTGCAGATGCGCGTGCTGCAGCCGTTCTATGAGCACCCGGCGTACATCGAGGCGCTGGCAGCCAGCGCCCGGCCGTACCTGGAGCAGGGTTACGACCACCTGCTACTGAGCTTCCa                                                                    >  1:79425/1‑149 (MQ=255)                                                          gCAAAACAGACGGTCAGCGAGCACCAGTTACCGTTGCAGATGCGCGTGCTGCAGCCGTTCTATGAGCACCCGGCGTACATCGAGGCACTGGCAGCCAGCGCCCGGCCGTACCTGGAGCAGGGTTACGACCACCTGCTACTGAGCTTCCa                                                                    >  7:255603/1‑149 (MQ=255)                                                          gCAAAACAGACGGTCAGCGAGCACCAGTTACCGTTGCAGATGCGCGTGCTGCAGCCGTTCTATGAGCACCCGGCGTACATCGAGGCACTGGCAGCCAGCGCCCGGCCGTACCTGGAGCAGGGTTACGACCACCTGCTACTGAGCTTCCa                                                                    >  5:151832/1‑149 (MQ=255)                                                            aaaaCAGACGGTCAGCGAGCACCAGTTACCGTTGCAGATGCGCGTGCTGCAGCCGTTCTATGAGCACCCGGCGTACATCGAGGCGCTGGCAGCCAGCGCCCGGCCGTACCTGGAGCAGGGTTACGACCACCTGCTACTGAGCTTCCATg                                                                  <  4:252241/149‑1 (MQ=255)                                                                       tCAGCGAGCACCAGTTACCGTTGCAGATGCGCGTGCTGCAGCCGTTCTATGAGCACCCGGCGTACATCGAGGCACTGGCAGCCAGCGCCCGGCCGTACCTGGAGCAGGGTTACGACCACCTGCTACTGAGCTTCCATGGTTTGCCGGaa                                                       <  6:151832/149‑1 (MQ=255)                                                                       tCAGCGAGCACCAGTTACCGTTGCAGATGCGCGTGCTGCAGCCGTTCTATGAGCACCCGGCGTACATCGAGGCACTGGCAGCCAGCGCCCGGCCGTACCTGGAGCAGGGTTACGACCACCTGCTACTGAGCTTCCATGGTTTGCCGGaa                                                       <  8:255603/149‑1 (MQ=255)                                                                           cGAGCACCAGTTACCGTTGCAGATGCGCGTGCTGCAGCCGTTCTATGAGCACCCGGCGTACATCGAGGCGCTGGCAGCCAGCGCCCGGCCGTACCTGGAGCAGGGTTACGACCACCTGCTACTGAGCTTCCATGGTTTGCCGGAACgg                                                    >  8:269980/1‑148 (MQ=255)                                                                             cgCACCAGTTACCGTTGCAGCTGCGCGTGCTGCAGCCTTGCTATGCGCACCCGGCGTACATCGTGGCGCTGGCAGCCAGCGCCCGGCCGTACCTGGAGCAGGGTTACGACCACCTGCTACTGAGCTTCCATGGTTTGCCGGAACGGCAc                                                 <  7:50301/148‑1 (MQ=255)                                                                             cgCACCAGTTACCGTTCCAGATGCGCGTGCGGCAGCCGTTCTATGAGCACCCGGCCTACATCGAGGCGCTGGCAGCCAGCGCCCGGCCGTACCTGGAGCAGGGTTACGACCACCTGCTACTGAGCTTCCATGGTTTGCCGGAACGGCAc                                                 <  8:349920/148‑1 (MQ=255)                                                                                aCCAGTTACCGTTGCAGATGCGCGTGCTGCAGCCGTTCTATGAGCACCCGGCGTACATCGAGGCGCTGGCAGCCAGCGCCCGGCCGTACCTGGAGCAGGGTTACGACCACCTGCTACTGAGCTTCCATGGTTTGCCGGAACGGCACCt                                               >  7:114364/1‑148 (MQ=255)                                                                                                     gggTGCTGCAGCCGTTTTATGAGCACCCGGCGTACTTCGAGGCGCTGCCAGCCAGCGCCCGGCCGTACCTGGAGCAGGGTTACGACCACCTGCTACTGAGCTTCCATGGTTTGCCGGAACGGCACCTGAAGAAACTGTTTCCGAAgggg                         <  2:79425/147‑1 (MQ=255)                                                                                                             cAGCCGTTCTATGAGCACCCGGCGTACATCGAGGCGCTGGCAGCCAGCGCCCGGCCGTACCTGGAGCAGGGTTACGACCACCTGCTACTGAGCTTCCATGGTTTGCCGGAACGGCACCTGAAGAAACTGTTTCCGAAGGGGGTCAAg                   <  7:269980/147‑1 (MQ=255)                                                                                                                  gTTCTATGAGCACCCGGCGTACATCGAGGCGCTGGCAGCCAGCGCCCGGCCGTACCTGGAGCAGGGTTACGACCACCTGCTACTGAGCTTCCATGGTTTGCCGGAACGGCACCTGAAGAAACTGTTTCCGAAGGGGGTCAAGCACGAcc            >  1:97976/1‑149 (MQ=255)                                                                                                                   ttCTATGAGCACCCGGCGTACATCGAGGCGCTGGCAGCCAGCGCCCGGCCGTACCTGGAGCAGGGTTACGACCACCTGCTACTGAGCTTCCATGGTTTGCCGGAACGGCACCTGAAGAAACTGttt                                  >  7:335828/1‑126 (MQ=255)                                                                                                                      tATGAGCACCCGGCGTACATCGAGGCGCTGGCAGCCAGCGCCCGGCCGTACCTGGAGCAGGGTTACGACCACCTGCTACTGAGCATCCATGGTTTGCCGGAACGGCACCTGAAGAAACTGTTTCCGAAGGGGGTCAAGCACGACCTgcg        >  3:60152/1‑149 (MQ=255)                                                                                                                        tGAGCACCCGGCGTACATCGAGGCGCTGGCAGCCAGCGCCCGGCCGTACCTGGAGCAGGGTTACGACCACCTGCTACTGAGCTTCCATGGTTTGCCGGAACGGCACCTGAAGAAACTGTTTCCGAAGGGGGTCAAGCACGACCTgcgcg      >  2:53128/1‑149 (MQ=255)                                                                                                                        tGAGCACCCGGCGTACATCGAGGCGCTGGCAGCCAGCGCCCGGCCGTACCTGGAGCAGGGTTACGACCACCTGCTACTGAGCTTCCATGGTTTGCCGGAACGGCACCTGAAGAAACTGTTTCCGAAGGGGGTCAAGCACGACCTgc         <  2:97976/146‑1 (MQ=255)                                                                                                                            ctcccGCCGTACATCGAGGCGCGGGCAGCCAGCGCCCGGCCGTACCTGGAGCAGGGTTACGACCACCTGCTACTGAGCTTCCATGGCTTGCCGGAACGGCACCTGAAGAAACTGTTTCCGAAGGGGGTCAAGCACGACCTGCGCGCTGc  <  2:285916/147‑1 (MQ=255)                                                                                                                                                |                                                                                                                                 TTGGCACCGCTTTATCCACAGTTCGCCGACAGCACGGTGACCACTGTGATCGAGCAGGCAAAACAGACGGTCAGCGAGCACCAGTTACCGTTGCAGATGCGCGTGCTGCAGCCGTTCTATGAGCACCCGGCGTACATCGAGGCGCTGGCAGCCAGCGCCCGGCCGTACCTGGAGCAGGGTTACGACCACCTGCTACTGAGCTTCCATGGTTTGCCGGAACGGCACCTGAAGAAACTGTTTCCGAAGGGGGTCAAGCACGACCTGCGCGCTGC  >  NC_002947/863227‑863498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑