Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 923,382 G→C 7.1% A604G (GCC→GGC)  PP_0804 ← protein secretion ABC transporter permease/ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947923,3820GC 7.1% 263.8 / 6.9 81A604G (GCC→GGC) PP_0804protein secretion ABC transporter permease/ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (35/40);  new base C (3/3);  total (38/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

GTCCAGCAACAGCACCTGCGGCTGGCGTACCAGCAGGCGCGACAGCACCAGGGCTTGGCGCTGGCCGCCAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAGGGTCGAGGTGGGCCATGG  >  NC_002947/923234‑923529
                                                                                                                                                    |                                                                                                                                                   
gTCCAGCAACAGCACCTGCGGCTGGCGTACCAGCAGGCGCGACAGCACCAGGGCTTGGCGCTGGCCGCCAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTgg                                                                                                                                                     >  8:280622/1‑149 (MQ=255)
   cAGCAACAGCACCTGCGGCTGGCGTACCAGCAGGCGCGACAGCACCAGGGCTTGGCGCTGGCCGCCAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTggcgg                                                                                                                                                  >  4:322499/1‑149 (MQ=255)
   cAGCAACAGCACCTGCGGCTGGCGTACCAGCAGGCGCGACAGCACCAGGGCTTGGCGCTGGCCGCCAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTggcgg                                                                                                                                                  >  2:249915/1‑149 (MQ=255)
    aGCAACAGCACCTGCGGCTGGCGTACCAGCAGGCGCGACAGCACCAGGGCTTGGCGCTGGCCGCCAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTggcggc                                                                                                                                                 <  7:350089/149‑1 (MQ=255)
        aCAGCACCTGCGGCTGGCGTACCAGCAGGCGCGACAGCACCAGGGCTTGGCGCTGGCCGCCAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAgc                                                                                                                                             >  6:12684/1‑149 (MQ=255)
               cTGCGGCTGGCGTACCAGCAGGCGCGACAGCACCAGGGCTTGGCGCTGGCCGCCAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGcca                                                                                                                                      >  4:17774/1‑149 (MQ=255)
                  cGGCTGGCGTACCAGCAGGCGCGACAGCACCAGGGCTTGGCGCTGGCCGCCAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGccacca                                                                                                                                   <  8:319230/149‑1 (MQ=255)
                  cGGCTGGCGTACCAGCAGGCGCGACAGCACCAGGGCTTGGCGCTGGCCGCCAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGccacca                                                                                                                                   <  7:350664/149‑1 (MQ=255)
                  cGGCTGGCGTACCAGCAGGCGCGACAGCACCAGGGCTTGGCGCTGGCCGCCAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGccac                                                                                                                                     <  8:85287/147‑1 (MQ=255)
                  cGGCTGGCGTACCAGCAGGCGCGACAGCACCAGGGCTTGGCGCTGGCCGCCAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGccac                                                                                                                                     <  7:329705/147‑1 (MQ=255)
                  cGGCTGGCGTACCAGCAGGCGCGACAGCACCAGGGCTTGGCGCTGGCCGCCAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGGCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGccac                                                                                                                                     <  5:65849/147‑1 (MQ=255)
                     cTGGCGTACCAGCAGGCGCGACAGCACCAGGGCTTGGCGCTGGCCGCCAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGtt                                                                                                                                <  1:249915/149‑1 (MQ=255)
                      tGGCGGACCAGCAGGCGCGACAGCACCAGGGCTTGGTGCTGGCCGCCAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGGGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTc                                                                                                                               <  7:338927/149‑1 (MQ=255)
                         cGTACCAGCAGGCGCGACAGCACCAGGGCTTGGCGCTGGCCGCCAGACAAGCCCAGGCCTCCTTCGAGGATCATGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTg                                                                                                                            <  5:235982/149‑1 (MQ=255)
                         cGTACCAGCAGGCGCGACAGCACCAGGGCTTGGCGCTGGCCGCCAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTg                                                                                                                            >  1:54287/1‑149 (MQ=255)
                           tACCAGCAGGCGCGACAGCACCAGGGCTTGGCGCTGGCCGCCAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGt                                                                                                                          <  5:12684/149‑1 (MQ=255)
                            aCCAGCAGGCGCGACAGCACCAGGGCTTGGCGCTGGCCGCCAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCCGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTc                                                                                                                         >  5:176302/1‑149 (MQ=255)
                            aCCAGCAGGCGCGACAGCACCAGGGCATGGCGCTGGCCGCCAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGCCGGCGGACGAAGTCCAGTGCCCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTc                                                                                                                         >  8:272303/1‑149 (MQ=255)
                               agcagGCGCGACAGCACCAGGGCTTGGCGCTGGCCGCCAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGct                                                                                                                      >  4:174114/1‑149 (MQ=255)
                                gcagGCGCGACAGCACCAGGGCTTGGCGCTGGCCGCCAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGctg                                                                                                                     <  3:287002/149‑1 (MQ=255)
                                          cAGCGCCAAGCCCTGGTGCTGGCCGCCAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCgg                                                                                                           >  6:131642/1‑149 (MQ=255)
                                           aGCTCCAGGGCTTGGCGCTGGCCGCCAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGCCGGCCAGGCCAGCGACCAGGAACTGGTGGCCGCGCTGGCCgcc                                                                                                          <  8:130075/149‑3 (MQ=25)
                                           aGCACCAGGGCTTGGCGCTGGCCGCCAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGc                                                                                                          <  1:170588/149‑1 (MQ=255)
                                              aCCAGGGCTTGGCGCTGGCCGCCAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCAcc                                                                                                       >  2:44874/1‑149 (MQ=255)
                                                        ggCGCTGGCCGCCAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAg                                                                                              <  6:176302/148‑1 (MQ=255)
                                                         gcgcTGGCCGCCAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAgg                                                                                             <  2:54287/148‑1 (MQ=255)
                                                               gccgccAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCtggcctggcc                                                                                                             <  3:46232/126‑1 (MQ=255)
                                                               gccgccAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCtggcctggcc                                                                                                             >  4:46232/1‑126 (MQ=255)
                                                               gccgccAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTccc                                                                                      >  6:59129/1‑149 (MQ=255)
                                                               gccgccAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTccc                                                                                      <  5:59129/149‑1 (MQ=255)
                                                                 cgccAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCTGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCg                                                                                                      <  7:2444/131‑1 (MQ=255)
                                                                 cgccAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCg                                                                                                      >  8:2444/1‑131 (MQ=255)
                                                                 cgccAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCg                                                                                     >  7:246194/1‑148 (MQ=255)
                                                                  gccAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGCCGGCCAGGCCAGCGACCAGGAACTGGTGGCCGCGCTGGCCGCCa                                                                                                         <  7:15317/127‑1 (MQ=21)
                                                                  gccAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGCCGGCCAGGCCAGCGACCAGGAACTGGTGGCCGCGCTGGCCGCCa                                                                                                         >  8:15317/1‑127 (MQ=21)
                                                                   ccAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAg                                                                                  <  5:131642/149‑1 (MQ=255)
                                                                       aCAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGCCGGCCAGGCCAGCGACCAGGAACTGGTGGCCGCGCTGGCCGCCAct                                                                                                       >  6:92525/1‑123 (MQ=18)
                                                                       aCAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGCCGGCCAGGCCAGCGACCAGGAACTGGTGGCCGCGCTGGCCGCCAct                                                                                                       <  5:92525/124‑2 (MQ=18)
                                                                         aaGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGcc                                                                            >  7:256355/1‑149 (MQ=255)
                                                                         aaGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGcc                                                                            <  3:174114/149‑1 (MQ=255)
                                                                           gCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTg                                                                                                                            <  1:68737/99‑1 (MQ=255)
                                                                           gCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTg                                                                                                                            >  2:68737/1‑99 (MQ=255)
                                                                           gCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGt                                                                          >  6:66815/1‑149 (MQ=255)
                                                                            cccAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTg                                                                         <  3:17774/149‑1 (MQ=255)
                                                                             ccAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTgg                                                                        <  7:135004/149‑1 (MQ=255)
                                                                             ccAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTg                                                                         >  2:58661/1‑148 (MQ=255)
                                                                                    tcctTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAg                                                                                              >  5:138492/1‑120 (MQ=255)
                                                                                    tcctTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAg                                                                                              <  6:138492/120‑1 (MQ=255)
                                                                                      ctTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGcc                                                                 <  7:280622/147‑1 (MQ=255)
                                                                                       ttCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTcg                                                              >  6:145232/1‑149 (MQ=255)
                                                                                            ggATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGACCAGTGCGCCACTGCCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGTGTGAGGTTATCCCGCAGGGTGCCGTGGAACAGCCTCGCGTAc                                                         >  8:354462/1‑149 (MQ=255)
                                                                                                 aaGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGt                                                                          >  7:273935/1‑127 (MQ=255)
                                                                                                 aaGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGt                                                                          <  8:273935/127‑1 (MQ=255)
                                                                                                   gTGGTCCATGCCCATGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCGACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGgca                                                  <  3:206761/149‑1 (MQ=255)
                                                                                                    tGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGgcag                                                 >  1:258864/1‑149 (MQ=255)
                                                                                                         cATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGc                                            >  8:88237/1‑149 (MQ=255)
                                                                                                         cATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGc                                            >  8:187934/1‑149 (MQ=255)
                                                                                                         cATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGc                                            >  6:295292/1‑149 (MQ=255)
                                                                                                            gCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGa                                         >  5:365716/1‑149 (MQ=255)
                                                                                                             cccTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGa                                         <  5:145232/148‑1 (MQ=255)
                                                                                                             cccTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGAc                                        <  7:88237/149‑1 (MQ=255)
                                                                                                             cccTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGAc                                        <  5:295292/149‑1 (MQ=255)
                                                                                                              ccTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACg                                       >  6:40213/1‑149 (MQ=255)
                                                                                                              ccTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGACGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACg                                       <  7:272303/149‑1 (MQ=255)
                                                                                                                  gggCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTc                                     <  8:246194/147‑1 (MQ=255)
                                                                                                                       ggcggcGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGgcgc                              <  5:66815/149‑1 (MQ=255)
                                                                                                                        gcggcgGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGgcg                               >  4:268225/1‑147 (MQ=255)
                                                                                                                         cggcggACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAg                            <  1:44874/149‑1 (MQ=255)
                                                                                                                             ggACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGt                          >  2:231259/1‑147 (MQ=255)
                                                                                                                                    gTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAGGGt                 <  8:256355/149‑1 (MQ=255)
                                                                                                                                       cAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAGGGTc                <  4:251013/147‑1 (MQ=255)
                                                                                                                                       cAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAGGGTc                >  3:39955/1‑147 (MQ=255)
                                                                                                                                       cAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAGGGTc                >  3:251013/1‑147 (MQ=255)
                                                                                                                                       cAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAGGGTCGa              <  3:268225/149‑1 (MQ=255)
                                                                                                                                        aGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAGGGTCGAg             >  7:238094/1‑149 (MQ=255)
                                                                                                                                        aGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAGGGTCGAg             <  2:258864/149‑1 (MQ=255)
                                                                                                                                         gTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAgg                   >  5:371766/1‑142 (MQ=255)
                                                                                                                                         gTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAgg                   <  6:371766/142‑1 (MQ=255)
                                                                                                                                         gTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAGGGTCGAgg            >  5:4860/1‑149 (MQ=255)
                                                                                                                                            cgcCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAg                            <  1:202426/130‑1 (MQ=255)
                                                                                                                                            cgcCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAg                            >  2:202426/1‑130 (MQ=255)
                                                                                                                                            cgcCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAGGGTCGAGGTgg         >  1:317133/1‑149 (MQ=255)
                                                                                                                                               cAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAGGGTCGAGGTGGGcc      <  1:58661/149‑1 (MQ=255)
                                                                                                                                               cAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAGGGTCGAGGTGGGc       >  1:47541/1‑148 (MQ=255)
                                                                                                                                                 gTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAGGGTCGAGGTGGGCCAt    <  5:40213/149‑1 (MQ=255)
                                                                                                                                                   ggcggcCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGTGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAGGGTCGAGGTGGGCCATgg  <  7:354462/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GTCCAGCAACAGCACCTGCGGCTGGCGTACCAGCAGGCGCGACAGCACCAGGGCTTGGCGCTGGCCGCCAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAGGGTCGAGGTGGGCCATGG  >  NC_002947/923234‑923529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑