Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 923,424 G→C 5.3% A590G (GCC→GGC)  PP_0804 ← protein secretion ABC transporter permease/ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947923,4240GC 5.3% 251.3 / 3.7 76A590G (GCC→GGC) PP_0804protein secretion ABC transporter permease/ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (35/37);  new base C (2/2);  total (37/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.10e-01

CAGCACCAGGGCTTGGCGCTGGCCGCCAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAGGGTCGAGGTGGGCCATGGCGATG‑CCGTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGC  >  NC_002947/923276‑923572
                                                                                                                                                    |                                                                                                                                                     
cAGCGCCAAGCCCTGGTGCTGGCCGCCAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCgg                                                                                                                                                       >  6:131642/1‑149 (MQ=255)
 aGCACCAGGGCTTGGCGCTGGCCGCCAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGc                                                                                                                                                      <  1:170588/149‑1 (MQ=255)
    aCCAGGGCTTGGCGCTGGCCGCCAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCAcc                                                                                                                                                   >  2:44874/1‑149 (MQ=255)
              ggCGCTGGCCGCCAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAg                                                                                                                                          <  6:176302/148‑1 (MQ=255)
               gcgcTGGCCGCCAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAgg                                                                                                                                         <  2:54287/148‑1 (MQ=255)
                     gccgccAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTccc                                                                                                                                  <  5:59129/149‑1 (MQ=255)
                     gccgccAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTccc                                                                                                                                  >  6:59129/1‑149 (MQ=255)
                       cgccAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCTGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCg                                                                                                                                                  <  7:2444/131‑1 (MQ=255)
                       cgccAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCg                                                                                                                                                  >  8:2444/1‑131 (MQ=255)
                       cgccAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCg                                                                                                                                 >  7:246194/1‑148 (MQ=255)
                        gccAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGCCGGCCAGGCCAGCGACCAGGAACTGGTGGCCGCGCTGGCCGCCa                                                                                                                                                     >  8:15317/1‑127 (MQ=21)
                        gccAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGCCGGCCAGGCCAGCGACCAGGAACTGGTGGCCGCGCTGGCCGCCa                                                                                                                                                     <  7:15317/127‑1 (MQ=21)
                         ccAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAg                                                                                                                              <  5:131642/149‑1 (MQ=255)
                             aCAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGCCGGCCAGGCCAGCGACCAGGAACTGGTGGCCGCGCTGGCCGCCAct                                                                                                                                                   <  5:92525/124‑2 (MQ=18)
                             aCAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGCCGGCCAGGCCAGCGACCAGGAACTGGTGGCCGCGCTGGCCGCCAct                                                                                                                                                   >  6:92525/1‑123 (MQ=18)
                               aaGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGcc                                                                                                                        >  7:256355/1‑149 (MQ=255)
                               aaGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGcc                                                                                                                        <  3:174114/149‑1 (MQ=255)
                                 gCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGt                                                                                                                      >  6:66815/1‑149 (MQ=255)
                                  cccAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTg                                                                                                                     <  3:17774/149‑1 (MQ=255)
                                   ccAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTgg                                                                                                                    <  7:135004/149‑1 (MQ=255)
                                   ccAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTg                                                                                                                     >  2:58661/1‑148 (MQ=255)
                                          tcctTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAg                                                                                                                                          <  6:138492/120‑1 (MQ=255)
                                          tcctTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAg                                                                                                                                          >  5:138492/1‑120 (MQ=255)
                                            ctTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGcc                                                                                                             <  7:280622/147‑1 (MQ=255)
                                             ttCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTcg                                                                                                          >  6:145232/1‑149 (MQ=255)
                                                  ggATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGACCAGTGCGCCACTGCCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGTGTGAGGTTATCCCGCAGGGTGCCGTGGAACAGCCTCGCGTAc                                                                                                     >  8:354462/1‑149 (MQ=255)
                                                       aaGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGt                                                                                                                      >  7:273935/1‑127 (MQ=255)
                                                       aaGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGt                                                                                                                      <  8:273935/127‑1 (MQ=255)
                                                         gTGGTCCATGCCCATGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCGACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGgca                                                                                              <  3:206761/149‑1 (MQ=255)
                                                          tGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGgcag                                                                                             >  1:258864/1‑149 (MQ=255)
                                                               cATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGc                                                                                        >  8:88237/1‑149 (MQ=255)
                                                               cATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGc                                                                                        >  6:295292/1‑149 (MQ=255)
                                                               cATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGc                                                                                        >  8:187934/1‑149 (MQ=255)
                                                                  gCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGa                                                                                     >  5:365716/1‑149 (MQ=255)
                                                                   cccTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGa                                                                                     <  5:145232/148‑1 (MQ=255)
                                                                   cccTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGAc                                                                                    <  5:295292/149‑1 (MQ=255)
                                                                   cccTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGAc                                                                                    <  7:88237/149‑1 (MQ=255)
                                                                    ccTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACg                                                                                   >  6:40213/1‑149 (MQ=255)
                                                                    ccTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGACGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACg                                                                                   <  7:272303/149‑1 (MQ=255)
                                                                        gggCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTc                                                                                 <  8:246194/147‑1 (MQ=255)
                                                                             ggcggcGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGgcgc                                                                          <  5:66815/149‑1 (MQ=255)
                                                                              gcggcgGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGgcg                                                                           >  4:268225/1‑147 (MQ=255)
                                                                               cggcggACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAg                                                                        <  1:44874/149‑1 (MQ=255)
                                                                                   ggACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGt                                                                      >  2:231259/1‑147 (MQ=255)
                                                                                          gTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAGGGt                                                             <  8:256355/149‑1 (MQ=255)
                                                                                             cAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAGGGTc                                                            <  4:251013/147‑1 (MQ=255)
                                                                                             cAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAGGGTc                                                            >  3:39955/1‑147 (MQ=255)
                                                                                             cAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAGGGTc                                                            >  3:251013/1‑147 (MQ=255)
                                                                                             cAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAGGGTCGa                                                          <  3:268225/149‑1 (MQ=255)
                                                                                              aGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAGGGTCGAg                                                         >  7:238094/1‑149 (MQ=255)
                                                                                              aGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAGGGTCGAg                                                         <  2:258864/149‑1 (MQ=255)
                                                                                               gTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAgg                                                               <  6:371766/142‑1 (MQ=255)
                                                                                               gTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAgg                                                               >  5:371766/1‑142 (MQ=255)
                                                                                               gTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAGGGTCGAgg                                                        >  5:4860/1‑149 (MQ=255)
                                                                                                  cgcCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAg                                                                        <  1:202426/130‑1 (MQ=255)
                                                                                                  cgcCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAg                                                                        >  2:202426/1‑130 (MQ=255)
                                                                                                  cgcCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAGGGTCGAGGTgg                                                     >  1:317133/1‑149 (MQ=255)
                                                                                                     cAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAGGGTCGAGGTGGGcc                                                  <  1:58661/149‑1 (MQ=255)
                                                                                                     cAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAGGGTCGAGGTGGGc                                                   >  1:47541/1‑148 (MQ=255)
                                                                                                       gTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAGGGTCGAGGTGGGCCAt                                                <  5:40213/149‑1 (MQ=255)
                                                                                                         ggcggcCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGTGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAGGGTCGAGGTGGGCCATgg                                              <  7:354462/149‑1 (MQ=255)
                                                                                                             gcCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAGGGTCGAGGTGGGCCATGGCGAt                                          <  6:365716/149‑1 (MQ=255)
                                                                                                                aGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAGGGGCGAGGTGGGCCATGGCGATG‑cc                                      <  7:187934/149‑1 (MQ=255)
                                                                                                                 gcgcGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGAc                                                                                    <  7:283310/103‑1 (MQ=255)
                                                                                                                 gcgcGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGAc                                                                                    >  8:283310/1‑103 (MQ=255)
                                                                                                                         accaGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAGGGTCGAGGTGGGCCATGGCGATG‑CCGTCCAGGCt                             >  2:129242/1‑149 (MQ=255)
                                                                                                                         accaGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAGGGTCGAGGTGGGCCATGGCGATG‑CCGTCCAGGCt                             >  2:41638/1‑149 (MQ=255)
                                                                                                                                 cTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAGGGTCGAGGTGGGCCATGGCGATG‑CCGTCCAGGCTGATCTCGc                     >  8:52084/1‑149 (MQ=255)
                                                                                                                                   ggTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAGGGTCGAGGTGGGCCATGGCGATG‑CCGTCCAGGCTGATCTCGccc                   >  1:230996/1‑149 (MQ=255)
                                                                                                                                   ggTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAGGGTCGAGGTGGGCCATGGCGATG‑CCGTCCAGGCTGATCTCGcc                    >  4:20496/1‑148 (MQ=255)
                                                                                                                                    gTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAGGGTCGAGGTGGGCCATGGCGATG‑CCGTCCAGGCTGATCTCGcc                    <  4:39955/147‑1 (MQ=255)
                                                                                                                                       gCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAGGGTCGAGGTGGGCCATGGCGATGACCGTCCAGGCTGATCTCGCCCTGc                <  7:52084/149‑1 (MQ=255)
                                                                                                                                             ctggccGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAGGGTCGAGGTGGGCCATGGCGATG‑CCGTCCAgg                               >  5:348431/1‑127 (MQ=255)
                                                                                                                                             ctggccGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAGGGTCGAGGTGGGCCATGGCGATG‑CCGTCCAgg                               <  6:348431/127‑1 (MQ=255)
                                                                                                                                              tggccGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAGGGTCGAGGTGGGCCATGGCGATG‑CCGTCCAGGCTGATCTCGCCCTGCAc              <  1:65816/143‑1 (MQ=255)
                                                                                                                                              tggccGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAGGGTCGAGGTGGGCCATGGCGATG‑CCGTCCAGGCTGATCTCGCCCTGCAc              >  2:65816/1‑143 (MQ=255)
                                                                                                                                                 ccGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAGGGTCGAGGTGGGCCATGGCGATG‑CCGTCCAGGCTGATCTCGCCCTGCACCAGATCCAt     <  2:230996/149‑1 (MQ=255)
                                                                                                                                                    gCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAGGGTCGAGGTGGGCCATGGCGATG‑CCGTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGc  >  2:172302/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CAGCACCAGGGCTTGGCGCTGGCCGCCAGACAAGCCCAGGCCTCCTTCGAGGATCAAGTGGTCCATGCCCTTGGGCAGGCGGCGGACGAAGTCCAGTGCGCCAGTGGCGGCCAGCGCGGCCACCAGTTCCTGGTCGCTGGCCTGGCCGGCACCGAGGGTGAGGTTTTCCCGCAGGGTGCCGTGGAACAGCCTCGCGTACTGCGGCAGCAGGCCGACGTCACGGCGCAGGTCAGCAGGGTCGAGGTGGGCCATGGCGATG‑CCGTCCAGGCTGATCTCGCCCTGCACCAGATCCATGGC  >  NC_002947/923276‑923572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑