Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 926,250 T→G 9.1% N126H (AAT→CAT)  PP_0805 ← outer membrane efflux protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947926,2500TG 9.1% 128.4 / 5.3 44N126H (AAT→CAT) PP_0805outer membrane efflux protein
Reads supporting (aligned to +/- strand):  ref base T (22/17);  new base G (2/2);  total (25/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

TGTGTCGCGGGCCAGGTCGTCGACCACCAGCAGGATATTGGCCTGGGCCCGGGCGGCGGCAGCCTGGGCCGCGTTGACGGCGCTGTCGACCTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGATGCTCGGGTGCCATTGCGCTGC  >  NC_002947/926107‑926397
                                                                                                                                               |                                                                                                                                                   
tgtgTCGCGGGCCAGGTCGTCGACCACCAGCAGGATATTGGCCTGGGCCCGGGCGGCGGCAGCCTGGGCCGCGTTGACGGCGCTGTCGACCTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGt                                                                                                                                                >  5:278404/1‑149 (MQ=255)
   gtCGCGGGCCAGGTCGTCGACCACCAGCAGGATATTGGCCTGGGCCCGGGCGGCGGCAGCCTGGGCCGCGTTGACGGCGCTGTCGACCTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTcgc                                                                                                                                             >  7:26428/1‑149 (MQ=255)
    tCGCGGGCCAGGTCGTCGACCACCAGCAGGATATTGGCCTGGGCCCGGGCGGCGGCAGCCTGGGCCGCGTTGACGGCGCTGTCGACCTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTcgcc                                                                                                                                            <  7:14466/149‑1 (MQ=255)
        gggCCAGGTCGTCGACCACCAGCAGGATATTGGCCTGGGCCCGGGCGGCGGCAGCCTGGGCCGCGTTGACGGCGCTGTCGACCTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCa                                                                                                                                        >  4:88157/1‑149 (MQ=255)
          gCCAGGTCGTCGACAACCAGCAGGATATTGGCCTGGGCCCGGGCGGCGGCAGCCTGGGCCGCGTTGACGGCGCTGTCGACCTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATa                                                                                                                                      <  6:311564/149‑1 (MQ=255)
               gtcgtcGACCACCAGCAGGATATTGGCCTGGGCCCGGGCGGCGGCAGCCTGGGCCGCGTTGACGGCGCTGTCGACCTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCg                                                                                                                                  <  8:26428/148‑1 (MQ=255)
                      accaccAGCAGGATATTGGCCTGGGCCCGGGCGGCGGCAGCCTGGGCCGCGTTGACGGCGCTGTCGACCTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATGGCGGCGACCGCAATAGcca                                                                                                                                  >  3:13264/1‑140 (MQ=255)
                      accaccAGCAGGATATTGGCCTGGGCCCGGGCGGCGGCAGCCTGGGCCGCGTTGACGGCGCTGTCGACCTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATGGCGGCGACCGCAATAGcca                                                                                                                                  <  4:13264/141‑2 (MQ=255)
                             gcagGATATTGGCCTGGGCCCGGGCGGCGGCAGCCTGGGCCGCGTTGACGGCGCTGTCGACCTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTg                                                                                                                   >  2:270836/1‑149 (MQ=255)
                                ggATATTGGCCTGGGCCCGGGCGGCGGCAGCCTGGGCCGCGTTGACGGCGCTGTCGACCTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCgg                                                                                                                <  3:88157/149‑1 (MQ=255)
                                   tatTGGCCTGGGCCCGGGCGGCGGCAGCCTGGGCCGCGTTGACGGCGCTGTCGACCTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTAtt                                                                                                                                                     >  8:125249/1‑109 (MQ=255)
                                   tatTGGCCTGGGCCCGGGCGGCGGCAGCCTGGGCCGCGTTGACGGCGCTGTCGACCTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTAtt                                                                                                                                                     <  7:125249/109‑1 (MQ=255)
                                    atTGGCCTGGGCCCGGGCGGCGGCAGCCTGGGCCGCGTTGACGGCGCTGTCGACCTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATAc                                                                                                            >  3:85025/1‑149 (MQ=255)
                                     ttGGCCTGGGCCCGGGCGGCGGCAGCCTGGGCCGCGTTGACGGCGCTGTCGACCTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATAc                                                                                                            <  2:190519/148‑1 (MQ=255)
                                        gCCTGGGCCCGGGCGGCGGCAGCCTGGGCCGCGTTGACGGCGCTGTCGACCTTGCCGAAGTCATAGCGCATTTGCTTGAGCGCCAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGGCATAACCGGTGCGGCTAccgcc                                                                                                        >  1:273886/1‑149 (MQ=255)
                                                      ggcggcAGCCTGGGCCGCGTTGACGGCGCTGTCGACCTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGt                                                                                          <  1:270836/149‑1 (MQ=255)
                                                              ccTGGGCCGCGTTGACGGCGCTGTCGACCTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATAcccc                                                                                  >  3:182182/1‑149 (MQ=255)
                                                               cTGGGCCGCGTTGACGGCGCTGTCGACCTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCg                                                                                 >  3:92639/1‑149 (MQ=255)
                                                                tGGGCCGCGTTGACGGCGCTGTCGACCTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCg                                                                                 >  7:292439/1‑148 (MQ=255)
                                                                                 gcTGTCGACCTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGc                                                               >  7:245281/1‑149 (MQ=255)
                                                                                       gACCTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTgg                                                                                            <  3:275916/114‑1 (MQ=255)
                                                                                       gACCTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTgg                                                                                            >  4:275916/1‑114 (MQ=255)
                                                                                         ccTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGccc                                                       >  1:20896/1‑149 (MQ=255)
                                                                                          cTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGAt                                                                 >  7:62802/1‑138 (MQ=255)
                                                                                          cTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGAt                                                                 <  8:62802/138‑1 (MQ=255)
                                                                                           ttGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGAt                                                                 >  4:326302/1‑137 (MQ=255)
                                                                                           ttGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGAt                                                                 >  4:320999/1‑137 (MQ=255)
                                                                                           ttGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGAt                                                                 <  3:326302/137‑1 (MQ=255)
                                                                                           ttGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGAt                                                                 <  3:320999/137‑1 (MQ=255)
                                                                                             gCCGAAGTCATAGAGCCTTTGCTTGAGCGACAGGTTCAGGGCCTGGCTCTTGCGGGCGCCGCCATAGCCGGTGTCCTATCCGGGGCGGCTCCCGCCGGACATCTGTGGGTAATACCCCGCACGGGCGCCGGTGATGCCTTCGCCCTGc                                                    <  2:273886/148‑1 (MQ=255)
                                                                                             gCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGACGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGc                                                    <  8:245281/148‑1 (MQ=255)
                                                                                               cGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATAGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACAGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTg                                                 >  6:291309/1‑149 (MQ=255)
                                                                                                gAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGt                                                <  4:182182/149‑1 (MQ=255)
                                                                                                       tAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGtt                                         >  8:118132/1‑149 (MQ=255)
                                                                                                          agCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCtgt                                                                                              >  4:259551/1‑93 (MQ=255)
                                                                                                            cATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCAGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTgg                                                                                            <  8:271756/93‑1 (MQ=255)
                                                                                                            cATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCAGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTgg                                                                                            >  7:271756/1‑93 (MQ=255)
                                                                                                                   ttGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCt                              >  2:37436/1‑148 (MQ=255)
                                                                                                                        cGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGa                        <  2:20896/149‑1 (MQ=255)
                                                                                                                              gTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGATGCTCg                  <  8:292439/149‑1 (MQ=255)
                                                                                                                               ttCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGATGCTCgg                 <  4:85025/149‑1 (MQ=255)
                                                                                                                               ttCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCATTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGATGCTCgg                 >  3:103755/1‑149 (MQ=255)
                                                                                                                                     gCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCAGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGATGCTCGGGTGCCa           >  8:51369/1‑149 (MQ=255)
                                                                                                                                       cTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGATGCTCGGGTGCCAtt         >  6:19456/1‑149 (MQ=255)
                                                                                                                                        cggCTATGGCGGCGACCGCAATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGc                                       >  1:34354/2‑118 (MQ=255)
                                                                                                                                        cggCTATGGCGGCGACCGCAATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGc                                       <  2:34354/117‑1 (MQ=255)
                                                                                                                                              ttGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGATGCTCGGGTGCCATTGCGct    <  4:56623/147‑1 (MQ=255)
                                                                                                                                              ttGCGGTAGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGCAAATCTGTCGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGATCCTCGGGTGCCATTGCGctgc  >  4:38059/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
TGTGTCGCGGGCCAGGTCGTCGACCACCAGCAGGATATTGGCCTGGGCCCGGGCGGCGGCAGCCTGGGCCGCGTTGACGGCGCTGTCGACCTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGATGCTCGGGTGCCATTGCGCTGC  >  NC_002947/926107‑926397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑