Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 926,256 C→G 8.9% D124H (GAC→CAC) ‡ PP_0805 ← outer membrane efflux protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947926,2560CG 8.9% 135.9 / 4.3 45D124H (GAC→CAC) ‡PP_0805outer membrane efflux protein
Reads supporting (aligned to +/- strand):  ref base C (22/18);  new base G (2/2);  total (25/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01

GTCGCGGGCCAGGTCGTCGACCACCAGCAGGATATTGGCCTGGGCCCGGGCGGCGGCAGCCTGGGCCGCGTTGACGGCGCTGTCGACCTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGATGCTCGGGTGCCATTGCGCTGCCTGCAG  >  NC_002947/926110‑926403
                                                                                                                                                  |                                                                                                                                                   
gtCGCGGGCCAGGTCGTCGACCACCAGCAGGATATTGGCCTGGGCCCGGGCGGCGGCAGCCTGGGCCGCGTTGACGGCGCTGTCGACCTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTcgc                                                                                                                                                   >  7:26428/1‑149 (MQ=255)
 tCGCGGGCCAGGTCGTCGACCACCAGCAGGATATTGGCCTGGGCCCGGGCGGCGGCAGCCTGGGCCGCGTTGACGGCGCTGTCGACCTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTcgcc                                                                                                                                                  <  7:14466/149‑1 (MQ=255)
     gggCCAGGTCGTCGACCACCAGCAGGATATTGGCCTGGGCCCGGGCGGCGGCAGCCTGGGCCGCGTTGACGGCGCTGTCGACCTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCa                                                                                                                                              >  4:88157/1‑149 (MQ=255)
       gCCAGGTCGTCGACAACCAGCAGGATATTGGCCTGGGCCCGGGCGGCGGCAGCCTGGGCCGCGTTGACGGCGCTGTCGACCTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATa                                                                                                                                            <  6:311564/149‑1 (MQ=255)
            gtcgtcGACCACCAGCAGGATATTGGCCTGGGCCCGGGCGGCGGCAGCCTGGGCCGCGTTGACGGCGCTGTCGACCTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCg                                                                                                                                        <  8:26428/148‑1 (MQ=255)
                   accaccAGCAGGATATTGGCCTGGGCCCGGGCGGCGGCAGCCTGGGCCGCGTTGACGGCGCTGTCGACCTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATGGCGGCGACCGCAATAGcca                                                                                                                                        <  4:13264/141‑2 (MQ=255)
                   accaccAGCAGGATATTGGCCTGGGCCCGGGCGGCGGCAGCCTGGGCCGCGTTGACGGCGCTGTCGACCTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATGGCGGCGACCGCAATAGcca                                                                                                                                        >  3:13264/1‑140 (MQ=255)
                          gcagGATATTGGCCTGGGCCCGGGCGGCGGCAGCCTGGGCCGCGTTGACGGCGCTGTCGACCTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTg                                                                                                                         >  2:270836/1‑149 (MQ=255)
                             ggATATTGGCCTGGGCCCGGGCGGCGGCAGCCTGGGCCGCGTTGACGGCGCTGTCGACCTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCgg                                                                                                                      <  3:88157/149‑1 (MQ=255)
                                 atTGGCCTGGGCCCGGGCGGCGGCAGCCTGGGCCGCGTTGACGGCGCTGTCGACCTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATAc                                                                                                                  >  3:85025/1‑149 (MQ=255)
                                  ttGGCCTGGGCCCGGGCGGCGGCAGCCTGGGCCGCGTTGACGGCGCTGTCGACCTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATAc                                                                                                                  <  2:190519/148‑1 (MQ=255)
                                     gCCTGGGCCCGGGCGGCGGCAGCCTGGGCCGCGTTGACGGCGCTGTCGACCTTGCCGAAGTCATAGCGCATTTGCTTGAGCGCCAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGGCATAACCGGTGCGGCTAccgcc                                                                                                              >  1:273886/1‑149 (MQ=255)
                                                   ggcggcAGCCTGGGCCGCGTTGACGGCGCTGTCGACCTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGt                                                                                                <  1:270836/149‑1 (MQ=255)
                                                           ccTGGGCCGCGTTGACGGCGCTGTCGACCTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATAcccc                                                                                        >  3:182182/1‑149 (MQ=255)
                                                            cTGGGCCGCGTTGACGGCGCTGTCGACCTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCg                                                                                       >  3:92639/1‑149 (MQ=255)
                                                             tGGGCCGCGTTGACGGCGCTGTCGACCTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCg                                                                                       >  7:292439/1‑148 (MQ=255)
                                                                              gcTGTCGACCTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGc                                                                     >  7:245281/1‑149 (MQ=255)
                                                                                    gACCTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTgg                                                                                                  >  4:275916/1‑114 (MQ=255)
                                                                                    gACCTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTgg                                                                                                  <  3:275916/114‑1 (MQ=255)
                                                                                      ccTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGccc                                                             >  1:20896/1‑149 (MQ=255)
                                                                                       cTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGAt                                                                       <  8:62802/138‑1 (MQ=255)
                                                                                       cTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGAt                                                                       >  7:62802/1‑138 (MQ=255)
                                                                                        ttGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGAt                                                                       <  3:320999/137‑1 (MQ=255)
                                                                                        ttGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGAt                                                                       >  4:320999/1‑137 (MQ=255)
                                                                                        ttGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGAt                                                                       >  4:326302/1‑137 (MQ=255)
                                                                                        ttGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGAt                                                                       <  3:326302/137‑1 (MQ=255)
                                                                                          gCCGAAGTCATAGAGCCTTTGCTTGAGCGACAGGTTCAGGGCCTGGCTCTTGCGGGCGCCGCCATAGCCGGTGTCCTATCCGGGGCGGCTCCCGCCGGACATCTGTGGGTAATACCCCGCACGGGCGCCGGTGATGCCTTCGCCCTGc                                                          <  2:273886/148‑1 (MQ=255)
                                                                                          gCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGACGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGc                                                          <  8:245281/148‑1 (MQ=255)
                                                                                            cGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATAGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACAGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTg                                                       >  6:291309/1‑149 (MQ=255)
                                                                                             gAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGt                                                      <  4:182182/149‑1 (MQ=255)
                                                                                                    tAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGtt                                               >  8:118132/1‑149 (MQ=255)
                                                                                                       agCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCtgt                                                                                                    >  4:259551/1‑93 (MQ=255)
                                                                                                         cATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCAGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTgg                                                                                                  <  8:271756/93‑1 (MQ=255)
                                                                                                         cATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCAGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTgg                                                                                                  >  7:271756/1‑93 (MQ=255)
                                                                                                                ttGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCt                                    >  2:37436/1‑148 (MQ=255)
                                                                                                                     cGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGa                              <  2:20896/149‑1 (MQ=255)
                                                                                                                           gTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGATGCTCg                        <  8:292439/149‑1 (MQ=255)
                                                                                                                            ttCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGATGCTCgg                       <  4:85025/149‑1 (MQ=255)
                                                                                                                            ttCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCATTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGATGCTCgg                       >  3:103755/1‑149 (MQ=255)
                                                                                                                                  gCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCAGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGATGCTCGGGTGCCa                 >  8:51369/1‑149 (MQ=255)
                                                                                                                                    cTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGATGCTCGGGTGCCAtt               >  6:19456/1‑149 (MQ=255)
                                                                                                                                     cggCTATGGCGGCGACCGCAATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGc                                             >  1:34354/2‑118 (MQ=255)
                                                                                                                                     cggCTATGGCGGCGACCGCAATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGc                                             <  2:34354/117‑1 (MQ=255)
                                                                                                                                           ttGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGATGCTCGGGTGCCATTGCGct          <  4:56623/147‑1 (MQ=255)
                                                                                                                                           ttGCGGTAGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGCAAATCTGTCGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGATCCTCGGGTGCCATTGCGctgc        >  4:38059/1‑149 (MQ=255)
                                                                                                                                              cGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGATGCTCGGGTGCCATTGCGctgcct      <  6:278404/148‑1 (MQ=255)
                                                                                                                                                 tCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGATGCTCGGGTGCCATTGCGCTGCCTgcag  >  4:126103/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GTCGCGGGCCAGGTCGTCGACCACCAGCAGGATATTGGCCTGGGCCCGGGCGGCGGCAGCCTGGGCCGCGTTGACGGCGCTGTCGACCTTGCCGAAGTCATAGAGCATTTGCTTGAGCGACAGGTTCAGGGCCTGGCTATTGCGGTCGCCGCCATAGCCGGTGTCATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGATGCTCGGGTGCCATTGCGCTGCCTGCAG  >  NC_002947/926110‑926403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑