Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 983,256 T→C 7.4% V89V (GTT→GTC)  fdx → ferredoxin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947983,2560TC 7.4% 153.3 / 2.6 54V89V (GTT→GTC) fdxferredoxin
Reads supporting (aligned to +/- strand):  ref base T (22/28);  new base C (2/2);  total (24/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

CTGCGGCGGCGTCAAGGCCTGTACCACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGTTGATGTACTTGAGATCGCAATCCAGCTTGCAGAAAGCAAGCCTGAG  >  NC_002947/983112‑983404
                                                                                                                                                |                                                                                                                                                    
cTGCGGCGGCGTCAAGGCCTGTACCACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTCTtcg                                                                                                                                                  >  6:8835/1‑149 (MQ=255)
   cggcggcgTCAAGGCCTGTACCACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTtcgtc                                                                                                                                                <  4:49807/148‑1 (MQ=255)
         cgTCAAGGCCTGTACCACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGAc                                                                                                                                          >  2:173225/1‑148 (MQ=255)
             aaGGCCTGTACCACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGGTGCCAGGTTTTCGTCGCTGACGAAGa                                                                                                                                     <  1:318735/149‑1 (MQ=255)
               ggCCTGTACCACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGAcc                                                                                                                                   >  2:54799/1‑149 (MQ=255)
               ggCCTGTACCACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGAcc                                                                                                                                   >  6:24066/1‑149 (MQ=255)
                gCCTGTACCACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCt                                                                                                                                  <  3:91595/149‑1 (MQ=255)
                gCCTGTACCACTTGCCACTGCATCGGGCGCAAGGGCTTCGATTCGCTCGATGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCt                                                                                                                                  <  7:236035/149‑1 (MQ=255)
                 ccTGTACCACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTg                                                                                                                                 <  8:319063/149‑1 (MQ=255)
                 ccTGTACCACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTCTTCGTCAGCGACGAAAACCTg                                                                                                                                 <  8:218957/149‑1 (MQ=255)
                  cTGTACCACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGa                                                                                                                                <  1:54799/149‑1 (MQ=255)
                  cTGTACCACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGa                                                                                                                                >  2:154728/1‑149 (MQ=255)
                   tGTACCACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGAc                                                                                                                               >  6:276043/1‑149 (MQ=255)
                     tACCACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCa                                                                                                                             <  5:276043/149‑1 (MQ=255)
                       ccACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTCGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCAt                                                                                                                            <  7:240605/148‑1 (MQ=255)
                        cACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCAt                                                                                                                            <  8:245776/147‑1 (MQ=255)
                        cACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCAt                                                                                                                            >  7:245776/1‑147 (MQ=255)
                          cTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCgag                                                                                                                        <  5:194194/149‑1 (MQ=255)
                             gCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCgaga                                                                                                                       <  1:58151/147‑1 (MQ=255)
                               cACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATccc                                                                                                                   >  5:285872/1‑149 (MQ=255)
                                aCTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATcccc                                                                                                                  <  2:214227/149‑1 (MQ=255)
                                aCTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATccc                                                                                                                   >  7:204727/1‑148 (MQ=255)
                                    cATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCaa                                                                                                                <  1:173225/147‑1 (MQ=255)
                                          gcgcAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTCTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCg                                                                                                         <  5:8835/148‑1 (MQ=255)
                                            gcAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCACTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCt                                                                                                       >  6:286397/1‑148 (MQ=255)
                                                 ggCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCa                                                                                                 >  7:93320/1‑149 (MQ=255)
                                                       gATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCAtgctgc                                                                                           <  3:280520/149‑1 (MQ=255)
                                                        aTTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGcc                                                                                          >  6:57032/1‑149 (MQ=255)
                                                         ttCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGcc                                                                                          >  3:56709/1‑148 (MQ=255)
                                                                gAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCAcc                                                                                  >  7:169795/1‑149 (MQ=255)
                                                                          aCGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAgg                                                                         <  8:93320/148‑1 (MQ=255)
                                                                          aCGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAg                                                                          <  1:154728/147‑1 (MQ=255)
                                                                             aGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCt                                                                     <  8:169795/149‑1 (MQ=255)
                                                                                tGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTa                                                                  >  3:144047/1‑149 (MQ=255)
                                                                                  gAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTa                                                                  <  4:56709/147‑1 (MQ=255)
                                                                                   aaGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACAt                                                               <  6:285872/149‑1 (MQ=255)
                                                                                     gagGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGa                                                             <  5:74502/149‑1 (MQ=255)
                                                                                       ggACATGCTGGACAAAGCCTGGGGCCTGGACGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGt                                                           >  5:156347/1‑149 (MQ=255)
                                                                                                 gACAAAGCGTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGGCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATgg                                                  <  4:337753/148‑1 (MQ=255)
                                                                                                 gACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATgg                                                  <  4:144047/148‑1 (MQ=255)
                                                                                                  aCAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGt                                                <  5:24066/149‑1 (MQ=255)
                                                                                                        ccTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGTTGATg                                           >  2:350222/1‑148 (MQ=255)
                                                                                                            gggCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGTTGATGTAc                                        <  5:57032/147‑1 (MQ=255)
                                                                                                            gggCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGTTGATGTACtt                                      >  3:93421/1‑149 (MQ=255)
                                                                                                              gCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGTTGATGTACTTga                                    <  8:204727/149‑1 (MQ=255)
                                                                                                                cTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGTTGATGTACTTgag                                   >  6:366281/1‑148 (MQ=255)
                                                                                                                      gcgcAATCGCGCCTGGGTTGCCAGGTTTTCGTCACTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGTTGATGTACTTGAGATCGCaa                            <  5:286397/149‑1 (MQ=255)
                                                                                                                          aaTCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGTTGATGTACTTGAGATCGCAATc                          >  5:234305/1‑147 (MQ=255)
                                                                                                                                          ccAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGTTGATGTACTTGAGATCGCAATCCAGCTTGCAGAaagcaa         <  5:366281/148‑1 (MQ=255)
                                                                                                                                           cAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGTTGATGTACTTGAGATCGCAATCCAGCTTGCAGAaagcaagc       >  8:289345/1‑149 (MQ=255)
                                                                                                                                            aGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGACTGTACATGAGTCTGAAATGGGTTGATGTACTTGAGATCGCAATCCAGCTTGCAGAAAGCAAGcc      >  6:314330/1‑149 (MQ=255)
                                                                                                                                             ggTCTTCGTCAGCGACGAAAACCTGACCATCGAGATCCCAAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATCGGTTGATGTACTTGAGATCGCAATCCAGCTTGCAGaaa             >  5:54723/1‑141 (MQ=255)
                                                                                                                                              gTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTa                                                                  >  7:252496/1‑87 (MQ=255)
                                                                                                                                              gTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTa                                                                  <  8:252496/87‑1 (MQ=255)
                                                                                                                                                tttCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGTTGATGTACTTGAGATCGCAATCCAGCTTGCAGAAAGCAAGCCTGAg  >  4:1009/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
CTGCGGCGGCGTCAAGGCCTGTACCACTTGCCACTGCATCGTGCGCAAGGGCTTCGATTCGCTCGAAGAGGCTGACGAGCTGGAAGAGGACATGCTGGACAAAGCCTGGGGCCTGGAAGCGCAATCGCGCCTGGGTTGCCAGGTTTTCGTCGCTGACGAAGACCTGACCATCGAGATCCCCAAGTATTCGCTCAACCATGCTGCCGAAGCACCGCACTGAGGGCCTGTACATGAGTCTGAAATGGGTTGATGTACTTGAGATCGCAATCCAGCTTGCAGAAAGCAAGCCTGAG  >  NC_002947/983112‑983404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑