| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 97,594 | A→C | 14.9% | intergenic (+23/+13) | PP_0090 → / ← PP_0091 | hypothetical protein/putative lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 97,594 | 0 | A | C | 14.9% | 84.2 / 12.3 | 47 | intergenic (+23/+13) | PP_0090/PP_0091 | hypothetical protein/putative lipoprotein |
| Reads supporting (aligned to +/- strand): ref base A (22/18); new base C (2/5); total (24/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.45e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.06e-01 | |||||||||||
GCCCAAGGGGTTTCGTCCTACACGCTGGAGATCGTGCCCAACAGCGAGGTCAAGGACCAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCC‑GGGGCTGCGAAGCAGCCCCACACTC‑ACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGCGATAGCGCTCGCCGGCCTTGAAGTTGT > NC_002947/97446‑97737 | tcccAAGGGGGTTCGTCCTACACGCTGGCGATCGTGCCCAACAGCGAGGGCAAGCACCAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAACAACGATCGATAAGCATCAGGGCCC‑GGGGCTGCGaa < 8:141809/148‑1 (MQ=255)gCCCAAGGGGTTTCGTCCTACACGCTGGAGATCGTGCCCAACAGCGAGGTCAAGGACCAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCC‑GGGGCTGCGaa < 6:249349/149‑1 (MQ=255) cAAGGGGTTTCGTCCTACACGCTGGAGATCGTGCCCAACAGCGAGGTCAAGGACCAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCC‑GGGGCTGCGAagca > 2:37957/1‑149 (MQ=255) cAAGGGGTTTCGTCCTACACGCTGGAGATCGTGCCCAACAGCGAGGTCAAGGACCAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCC‑GGGGCTGCGAag > 1:130007/1‑147 (MQ=255) ggggTTTCGTCCTACACGCTGGAGATCGTGCCCAACAGCGAGGTCAAGGACCAGAACATGGTCGACGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCC‑GGGGCTGCGAAGCAGcc > 5:259487/1‑149 (MQ=255) ggggTTTCGTCCTACACGCGTGAGATCGTGCCCAACAGCGATGTCAACGAACAGAACATGGTCGTCGTGACCTGCGATGGCTGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCC‑GGGGCTGCTTCGCAGcc < 2:353908/149‑1 (MQ=255) ggggTTTCGGCCTACACGCTGGATCTCGTGCCCAACAGCGTGGTGAAGGACCAGAACTTGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCC‑GGGGCTGCTTCGCAGcc < 2:359480/149‑1 (MQ=255) tCGTCCTACACGCTGGAGATCGTGCCCAACAGCGAGGTCAAGGACCAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCC‑GGGGCTGCGAAGCAGCCCCacac > 4:302053/1‑149 (MQ=255) ccacGCTGGAGATCGTGCCCAACAGCGAGGTCAAGGACCAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGCTCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCC‑GGGGCTGCGAAGCAGCCCCACACTC‑ACgg > 7:241842/2‑148 (MQ=255) gCCCAACAGCGAGGTCAAGGACCAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCC‑GGGGCTGCGAAGCAGCCCCACACTC‑ACGGGATGCAGAACACTTCGc > 2:88803/1‑149 (MQ=255) cGAGGTCAAGGACCAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCC‑GGGGCTGCTTCGCAGccc < 1:40914/112‑1 (MQ=255) cGAGGTCAAGGACCAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCC‑GGGGCTGCTTCGCAGccc > 2:40914/1‑112 (MQ=255) cGAGGTCAAGGACCAGAACATGGTCGGCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAACAACGATCGATAAGCATCAGGGCCC‑GGGGCGGCGAAGCAGCCCCACACTC‑ACGGGATGCAGAACACTTCGCTAGGCTCGt < 8:182686/149‑1 (MQ=255) tCAAGGACCAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCC‑GGGGCTGCGAAGCAGCCCCACACAA‑ACGGGATGAAGAAAACTTAGCGAGGCTCGTTaac > 8:235062/1‑148 (MQ=255) ccAGAACATGGTGGTCGGGACCTGCGTTGGGGGGACGAAGAAGATCATCTATCAGAAGAAGGATCTATAAGCATCAGGGCCC‑GGGGCTGCGAAGCAGCCCCACACTC‑ACGGGa < 2:69014/113‑1 (MQ=255) ccAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCC‑GGGGCTGCGAAGCAGCCCCACACTC‑ACGGGa > 1:69014/1‑113 (MQ=255) ccAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCC‑GGGGCTGCGAAGCAGCCCCACACTC‑ACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCt > 1:92580/1‑149 (MQ=255) cAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCC‑GGGGCTGCGaa < 8:232359/92‑1 (MQ=255) cAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCC‑GGGGCTGCGaa > 7:232359/1‑92 (MQ=255) gAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCC‑GGGGCTGCGAAGCAGCCCCACACTC‑ACGGGATGCAGAACACTTc > 5:216515/1‑123 (MQ=255) caaCATGGGCGTCGGGACCTGCTATGGCGGGACTAATAAGATAATATATCATAATAACTATCTATAAGCATCAGGGCCC‑GGGGCTGCTAAGCAGCCCCACACTC‑ACGGGATGCAGAACACTTc < 6:216515/122‑1 (MQ=255) ggTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCC‑GGGGCTGCGAAGCAGCCCCACACTC‑ACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTgg > 1:229610/1‑149 (MQ=255) ggcgGGACCGGCTAGGGCGGGACGAAGATCATAATCTATCAGAAGAACGATCGAAACGCATCTGGGCCG‑GGGGCTGCTAAGCAGCCCCACACTC‑ACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTc < 8:241842/147‑1 (MQ=255) tcgGGACCTGCGTTGGCGGGACGAAGAAGATCATATATCAGAAGAACTATCTATAAGCATCAGGGCCC‑GGGGCTGCTAAGCAGCCCCACACTC‑ACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTg < 2:289996/137‑1 (MQ=255) tcgGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCC‑GGGGCTGCGAAGCAGCCCCACACTC‑ACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTg > 1:289996/1‑137 (MQ=255) tcgGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCC‑GGGGCTGCGAAGCAGCCCCACACTC‑ACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCa < 3:341345/149‑1 (MQ=255) gggACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCC‑GGGGCTGCGAAGCAGCCCCACACTC‑ACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCat < 3:302053/148‑1 (MQ=255) gtaCCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAACAACGATCGATAAGCAGCAGGGCCC‑GGGGCTGCGAAGCAGCCCCACACTC‑ACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCatat < 5:298548/147‑1 (MQ=255) ccTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCC‑GGGGCTGCGAAGCAGCCCCACACTC‑ACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATagc < 5:170982/149‑1 (MQ=255) tGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCC‑GGGGCTTCGAAGCACCCCCACACAC‑ACGGGATTCAGAACACATAGCTAGGCTCGTTGAACACCAACATGCGGTTGGCGTCAaatat > 3:12928/1‑145 (MQ=255) tGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCC‑GGGGCTGCGAAGGGGCCCCCAACTC‑ACGGGATGCAGAGCGCTTGGCTAGGGTGGTTCGCCTCCACCTTTCGGTTGGGGTCGTATaggtg > 8:355934/1‑146 (MQ=255) ctttGGCGGTACTAATAATATAATCTATAATAATAACGATCTATATGCATCAGGGCCC‑GGGGGTGCTAAGCAGCCCCACACTC‑ACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATagca < 4:12928/143‑1 (MQ=255) cGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCC‑GGGGCTGCGAAGCAGCCCCACACTCAACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATagca < 1:110530/147‑1 (MQ=255) gCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCC‑GGGGCTGCGAAGCAGCCCCACACTC‑ACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTg < 2:229610/148‑1 (MQ=255) gaagaaGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCC‑GGGGCTGCGAAGCAGCCCCACACTC‑ACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCa > 2:51475/1‑149 (MQ=255) gaagaaGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCC‑GGGGCTGCGAAGCAGCCCCACACTC‑ACGGGATGCAGAACACTTCGCGAGGCGAGTTCACCACCACCTTGCGGTTGGCGTCATAAAGCAGCACCTGCGGccac > 2:120162/1‑145 (MQ=255) agaagaTCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCC‑GGGGCTGCGAAGCAGCCCCACACTC‑ACGGGATGCAGAACACATAGCGAGGCTCGTTCACCCCCAACATGCGGTTGGCGTCATATAGCCGCACCAGCGGCTCCaa > 4:290176/1‑147 (MQ=255) gaagaTCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCC‑GGGGCTGCGAAGCAGCCCCACACTC‑ACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCa < 1:37957/149‑1 (MQ=255) tcataTATCAGAAGAACGATCGATAAGCATCAGGGCCC‑GGGGCTGCGAAGCAGCCCCACACTC‑ACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGgc < 3:290176/149‑1 (MQ=255) aTCAGAAGAACGATCGATAAGCATCAGGGCCC‑GGGGCTGCGAAGCAGCCCCACACTC‑ACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCcgc > 7:211848/1‑149 (MQ=255) tCAGAAGAACGATCGATAAGCATCAGGGCCC‑GGGGCTGCGAAGCAGCCCCACACTC‑ACGGGATGCAGAACACATCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCcgcg > 2:9686/1‑149 (MQ=255) gaagaaCGATCGATAAGCATCAGGGCCC‑GGGGCTGCGAAGCAGCCCCACACTC‑ACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCtt > 7:176337/1‑149 (MQ=255) gaagaaCGATCGATAAGCATCAGGGCCC‑GGGGCAGCGAAGCAGCCCCACACTC‑ACGGGATGCAGAACACATCGCGAGGGTCGTTCACCACCACCTTGCCGTTGGCCTCATATAGCAGCACCAGCGGCACCATCAACCGCGCCCGCGCt > 8:18482/1‑148 (MQ=255) ctatctatAAGCATCCGGCCCG‑GGGGCTGCTAAGCAGCCCCACACTC‑ACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGc < 2:130007/147‑1 (MQ=255) cgatcgatAAGCATCAGGGCCC‑GGGGCTGCGAAGCAGCCCCACACTC‑ACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGc < 1:51475/149‑1 (MQ=255) gggCCC‑GGGGCTGCGAAGCAGCCCCCCCCGC‑ACCGGGTGCAGAACACATAGGTAGGGGCGTTGACCACCACCATGCGGTTGGGGTCATATATCAGCCCCTGCGGCTCCAACACCCGCGCCCGCGCGTACCGGCGATAGCCCGGGCCGGc > 4:345297/1‑149 (MQ=255) ggCCCGGGGGCGGCTAAGCCGCCCCACACTC‑ACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGCGATAGCGCTCGCCGGc < 1:165339/149‑1 (MQ=255) ggCCC‑GGGGCTGCGAAGCAGCCCCACACTC‑ACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGCGATAGCGCTCGCCg < 5:108300/146‑1 (MQ=255) ggCCC‑GGGGCTGCGAAGCAGCCCCACACTC‑ACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGCGATAGCGCTCGCCg > 6:108300/1‑146 (MQ=255) ggggCTGCTTCGCAGCCCCACACTC‑ACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAgg > 7:352655/1‑126 (MQ=255) ggggCTGCTTCGCAGCCCCACACTC‑ACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAgg < 8:352655/126‑1 (MQ=255) gcggCGACGCAGCCCCACACTC‑ACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGCGATAGCGCTCGCCGGCCTTGAAGtt < 3:188456/146‑1 (MQ=255) tGCGAAGCAGCCCCACACTC‑ACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGCGATAGCGCTCGCCGGCCTTGAAGTTGt > 2:70845/1‑149 (MQ=255) | GCCCAAGGGGTTTCGTCCTACACGCTGGAGATCGTGCCCAACAGCGAGGTCAAGGACCAGAACATGGTCGTCGGGACCTGCGATGGCGGGACGAAGAAGATCATCTATCAGAAGAACGATCGATAAGCATCAGGGCCC‑GGGGCTGCGAAGCAGCCCCACACTC‑ACGGGATGCAGAACACTTCGCTAGGCTCGTTCACCACCACCTTGCGGTTGGCGTCATATAGCAGCACCTGCGGCTCCATCACCGGCGCCCGCGCTTCCAGGCGATAGCGCTCGCCGGCCTTGAAGTTGT > NC_002947/97446‑97737 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |