Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 999,287 C→T 8.3% G278R (GGG→AGG)  PP_0861 ← outer membrane ferric siderophore receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947999,2870CT 8.3% 132.6 / 2.6 48G278R (GGG→AGG) PP_0861outer membrane ferric siderophore receptor
Reads supporting (aligned to +/- strand):  ref base C (17/27);  new base T (2/2);  total (19/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.36e-01

GATGGTCAGCGAGTCGGTCAGGTCGTGCTCGACGGCGAAGGTTGCGATATCCACTCGGGTCTTGCGGAAGTCGCGGCCGGTCAGGCCGTAGAAGTTGTCACTGTCGCCGCCATCATTCGGCTTGCTCGGGTGTGCCGAGGTGCGCCCGGCGCTGCCGTTGGTCGGGATGGTGTAAGGGATGCCCGAATCCGGCAGGTCGTCGCTTTCCAGGTGGTAGTAGTCAAGGTTGACGCGGGTCGGCGTGCCCAGGCCAAAGGCCAGCGACGGGGCGATACCCCAGCGGTCGTAGTTGAC  >  NC_002947/999141‑999434
                                                                                                                                                  |                                                                                                                                                   
gATGGTCAGCGAGTCGGTCAGGTCGTGCTCGACGGCGAAGGTTGCGATATCCACTCGGGTCTTGCGGAAGTCGCGGCCGGTCAGGCCGTAGAAGTTGTCACTGTCGCCGCCATCATTCGGCTTGCTCGGGTGTGCCGAGGTGCGCCCgg                                                                                                                                                   >  7:4849/1‑149 (MQ=255)
       aGCGAGTCGGTCAGGTCGTGCTCGACGGCGAAGGTTGCGATATCCACTCGGGTCTTGCGGAAGTCGCGGCCGGTCAGGCCGTAGAAGTTGTCACTGTCGCCGCCATCATTCGGCTTGCTCGGGTGTGCCGAGGTGCGCCCGGCGCTGcc                                                                                                                                            <  5:338450/149‑1 (MQ=255)
         cGAGTCGGTCAGGTCGTGCTCGACGGCGAAGGTTGCGATATCCACTCGGGTCTTGCGGAAGTCGCGGCCGGTCAGGCCGTAGAAGTTGTCACTGTCGCCGCCATCATTCGGCTTGCTCGGGTGTGCCGAGGTGCGCCCGGCGCTGCCGt                                                                                                                                          <  4:117757/149‑1 (MQ=255)
         cGAGTCGGTCAGGTCGTGCTCGACGGCGAAGGTTGCGATATCCACTCGGGTCTTGCGGAAGTCGCGGCCGGTCAGGCCGTAGAAGTTGTCACTGTCGCCGCCATCATTCGGCTTGCTCGGGTGTGCCGAGGTGCGCCCGGCGCTGCCGt                                                                                                                                          <  2:231544/149‑1 (MQ=255)
           aGTCGGTCAGGTCGTGCTCGACGGCGAAGGTTGCGATATCCACTCGGGTCTTGCGGAAGTCGCGGCCGGTCAGGCCGTAGAAGTTGTCACTGTCGCCGCCATCATTCGGCTTGCTCGGGTGTGCCGAGGTGCGCCCGGCGCTGCCGTTg                                                                                                                                        <  4:327600/149‑1 (MQ=255)
            gTCGGTCAGGTCGTGCTCGACGGCGAAGGTTGCGATATCCACTCGGGTCTTGCGGAAGTCGCGGCCGGTCAGGCCGTAGAAGTTGTCACTGTCGCCGCCATCATTCGGCTTGCTCGGGTGTGCCGAGGTGCGCCCGGCGCTGCCGTTgg                                                                                                                                       >  5:178603/1‑149 (MQ=255)
                    ggTCGTGCTCGACGGCGAAGGTTGCGATATCCACTCGGGTCTTGCGGAAGTCGCGGCCGGTCAGGCCGTAGAAGTTGTCACTGTCGCCGCCATCATTCGGCTTGCTCGGGTGTGCCGAGGTGCGCCCGGCGCTGCCGTTGGTCGGGATg                                                                                                                               <  6:178603/149‑1 (MQ=255)
                      tCGTGCTCGACGGCGAAGGTTGCGATATCCACTCGGGTCTTGCGGAAGTCGCGGCCGGTCAGGCCGTAGAAGTTGTCACTGTCGCCGCCATCATTCGGCTTGCTCGGGTGTGCCGAGGTGCGCCCGGCGCTGCCGTTGGTCGGGATGgt                                                                                                                             >  5:151653/1‑149 (MQ=255)
                         tGCTCGACGGCGAAGGTTGCGATATCCACTCGGGTCTTGCGGAAGTCGCGGCCGGTCAGGCCGTAGAAGTTGTCACTGTCGCCGCCATCATTCGGCTTGCTCGGGTGTGCCGAGGTGCGCCCGGCGCTGCCGTTGGTCGGGATGgtgt                                                                                                                           >  5:323121/1‑148 (MQ=255)
                           cTCGACGGCGAAGGTTGCGATATCCACTCGGGTCTTGCGGAAGTCGCGGCCGGTCAGGCCGTAGAAGTTGTCACTGTCGCCGCCATCATTCGGCTTGCTCGGGTGTGCCGAGGTGCGCCCGGCGCTGCCGTTGGTCGGGATGGTGTAAg                                                                                                                        <  7:118036/149‑1 (MQ=255)
                              gACGGCGAAGGTTGCGATATCCACTCGGGTCTTGCGGAAGTCGCGGCCGGTCAGGCCGTAGAAGTTGTCACTGTCGCCGCCATCATTCGGCTTGCTCGGGTGTGCCGAGGTGCGCCCGGCGCTGCCGTTGGTCGGGATGGTGTAAggg                                                                                                                      <  8:133993/148‑1 (MQ=255)
                                 ggCGAAGGTTGCGATATCCACTCGGGTCTTGCGGAAGTCGCGGCCGGTCAGGCCGTAGAAGTTGTCACTGTCGCCGCCATCATTCGGCTTGCTCGGGTGTGCCGAGGTGCGCCCGGCGCTGCCGTTGGTCGGGATGGTGTAAGGGATGc                                                                                                                  <  5:350241/149‑1 (MQ=255)
                                   cGAAGGTGTCGATATCCACTCGGGTCTTGCGGAAGTCGCTGCCGGTCAGGCCGTAGAAGTTGTCACTGTCGCCGCCATCATTCGTCTTGCTCGGGTGTGCCGAGGTGCTCCCGGCGCTGCCGTTGGTCGGGATGGTGTAAGGGATTccc                                                                                                                <  3:141990/149‑1 (MQ=255)
                                    gAAGGTTGCGATATCCACTCGGGTCTTGCGGAAGTCGCGGCCGGTCAGGCCGTAGAAGTTGTCACTGTCGCCGCCATCATTCGGCTTGCTCGGGTGTGCCGAGGTGCGCCCGGCGCTGCCGTTGGTCGGGATGGTGTAAGGGATGccc                                                                                                                >  3:350392/1‑148 (MQ=255)
                                            cGATATCCACTCGGGTCTTGCGGAAGTCGCGGCCGGTCAGGCCGTAGAAGTTGTCACTGTCGCCGCCATCATTCGGCTTGCTCGGGTGTGCCGAGGTGCGCCCGGCGCTGCCGTTGGTCGGGATGGTGTAAGGGATGCCCGAATCCgg                                                                                                        >  5:75441/1‑148 (MQ=255)
                                             gATATCCACTCGGGTCTTGCGGAAGTCGCGGCCGGTCAGGCCGTAGAAGTTGTCACTGTCGCCGCCATCATTCGGCTTGCTCGGGTGTGCCGAGGTGCGCCCGGCGCTGCCGTTGGTCGGGATGGTGTAAGGGATGCCCGAATCCGGCa                                                                                                      <  1:372240/149‑1 (MQ=255)
                                             gATATCCACTCGGGTCTTGCGGAAGTCGCGGCCGGTCAGGCCGTAGAAGTTGTCACTGTCGCCGCCATCATTCGGCTTGCTCGGGTGTGCCGAGGTGCGCCCGGCGCTGCCGTTGGTCGGGATGGTGTAAGGGATGCCCGAATCCGGCa                                                                                                      <  3:353525/149‑1 (MQ=255)
                                                  ccACTCGGGTCTTGCGGAAGTCGCGGCCGGTCAGGCCGTAGAAGTTGTCACTGTCGCCGCCATCATTCGGCTTGCTCGGGTGTGCCGAGGTGCGCCCGGCGCTGCCGTTGGTCGGGATGGTGTAAGGGATGCCCGAATCCGGCAGgtcg                                                                                                 >  2:176939/1‑149 (MQ=255)
                                                   cACTCGGGTCTTGCGGAAGTCGCGGCCGGTCAGGCCGTAGAAGTTGTCACTGTCGCCGCCATCATTCGGCTTGCTCGGGTGTGCCGAGGTGCGCCCGGCGCTGCCGTTGGACGGGATGGTGTAAGGGATGCCCGAATCCGGCAGgtcg                                                                                                 <  8:4849/148‑1 (MQ=255)
                                                      tCGGGTCTTGCGGAAGTCGCGGCCGGTCAGGCCGTAGAAGTTGTCACTGTCGCCGCCATCATTCGGCTTGCTCGGGTGTGCCGAGGTGCGCCCGGCGCTGCCGTTGGTCGGGATGGTGTAAGGGATGCCCGAATCCGGCAGGTCGTCGc                                                                                             >  1:240370/1‑149 (MQ=255)
                                                          gTCTTGCGGAAGTCGCGGCCGGTCAGGCCGTAGAAGTTGTCACTGTCGCCGCCATCATTCGGCTTGCTCGGGTGTGCCGAGGTGCGCCCGGCGCTGCCGTTGGTCGGGATGGTGTAAGGGATGCCCGAATCCGGCAGGTCGTCGCTTTc                                                                                         <  6:75441/149‑1 (MQ=255)
                                                               gCGGAAGTCGCGGCCGGTCAGGCCGTAGAAGTTGTCACTGTCGCCGCCATCATTCGGCTTGCTCGGGTGTGCCGAGGTGCGCCCGGCGCTGCCGTTGGTCGGGATGGTGTAAGGGATGCCCGAATCCGGCAGGTCGTCGCTTTCCa                                                                                       <  4:350392/146‑1 (MQ=255)
                                                               gCGGAAGTCGCGGCCGGTCAGGCCGTAGAAGTTGTCACTGTCGCCGCCATCATTCGGCTTGCTCGGGTGTGCCGAGGTGCGCCCGGCGCTGCCGTTGGTCGGGATGGTGTAAGGGATGCCCGAATCCGGCAGGTCGTCGCTTTCCAggt                                                                                    >  7:10892/1‑149 (MQ=255)
                                                                cGGAAGTCGCGGCCGGTCAGGCCGTAGAAGTTGTCACTGTCGCCGCCATCATTCGGCTTGCTCGGGTGTGCCGAGGTGCGCCCGGCGCTGCCGTTGGTCGGGATGGTGTAAGGGATGCCCGAATCCGGCAGGTCGTCGCTTTCCAggtg                                                                                   <  5:286726/149‑1 (MQ=255)
                                                                cGGAAGTCGCGGCCGGTCAGGCCGTAGAAGTTGTCACTGTCGCCGCCATCATTCGGCTTGCTCGGGTGTGCCGAGGTGCGCCCGGCGCTGCCGTTGGTCGGGATGGTGTAAGGGATGCCCGAATCCGGCAGGTCGTCGCTTTCCAggt                                                                                    <  7:50537/148‑1 (MQ=255)
                                                                   aaGTCGCGGCCGGTCAGGCCGTAGAAGTTGTCACTGTCGCCGCCATCATTCGGCTTGCTCGGGTGTGCCGAGGTGCGCCCGGCGCTGCCGTTGGTCGGGATGGTGTAAGGGATGCCCGAATCCGGCAGGTCGTCGCTTTCCAGGTGgta                                                                                <  2:4972/149‑1 (MQ=255)
                                                                   aaGTCGCGGCCGGTCAGGCCGTAGAAGTTGTCACTGTCGCCGCCATCATTCGGCTTGCTCGGGTGTGCCGAGGTGCGCCCGGCGCTGCCGTTGGTCGGGATGGTGTAAGGGATGCCCGAATCCGGCAGGTCGTCGCTTTCCAGGTGgta                                                                                <  6:151653/149‑1 (MQ=255)
                                                                           gCCGGTCAGGCCGTAGAAGTTGTCACTGTCGCCGCCATCATTCGGCTTGCTCGGGTGTGCCGAGGTGCGCCCGGCGCTGCCGTTGGTCGGGATGGTGTAAGGGATGCCCGAATCCGGCAGGTCGTCGCTTTCCAGGTGGTAGTAGTCaa                                                                        <  1:171048/149‑1 (MQ=255)
                                                                                        tAGAAGTTGTCACTGTCGCCGCCATCATTCGGCTTGCTCGGGTGTGCCGAGGTGCGCCCGGCGCCGCCGTTGGTCGGGATGGTGTAAGGGATGCCCGAATCCGGCAGGTCGTCGCTTTCCAGGTGGTAGTAGTCAAGGTTGACGCGGGt                                                           >  3:307483/1‑149 (MQ=255)
                                                                                             gTTGTCACTGTCGCCGCCATCATTCGGCTTGCTCGGGTGTGCCGAGGTGCGCCCGGCGCTGCCGTTGGTCGGGATGGTGTAAGGGATGCCCGAATCCGGCAGGTCGTCGCTTTCCAGGTGGTAGTAGTc                                                                          <  3:114897/129‑1 (MQ=255)
                                                                                             gTTGTCACTGTCGCCGCCATCATTCGGCTTGCTCGGGTGTGCCGAGGTGCGCCCGGCGCTGCCGTTGGTCGGGATGGTGTAAGGGATGCCCGAATCCGGCAGGTCGTCGCTTTCCAGGTGGTAGTAGTc                                                                          >  4:114897/1‑129 (MQ=255)
                                                                                              ttGTCACTGTCGCCGCCATCATTCGGCTTGCTCGGGTGTGCCGAGGTGCGCCCGGCGCTGCCGTTGGTCGGGATGGTGTAAGGGATGCCCGAATCCGGCAGGTCGTCGCTTTCCAGGTGGTAGTAGTCAAGGTTGACGCGGGTCGGCGt                                                     <  1:176939/149‑1 (MQ=255)
                                                                                                    cTGTCGCCGCCATCATTCGGCTTGCTCGGGTGTGCCGAGGTGCGCCCGGCGCTGCCGTTGGTCGGGATGGTGTAAGGGATGCCCGAATCCGGCAGGTCGTCGCTTTCCAGGTGGTAGTAGTCAAGGTTGACGCGGGTCGGCGTGCCCAg                                               <  8:10892/149‑1 (MQ=255)
                                                                                                       tCGCCGCCATCATTCGGCTTGCTCGGGTGTGCCGAGGTGCGCCCGGCGCTGCCGTTGGTCGGGATGGTGTAAGGGATGCCCGAATCCGGCAGGTCGTCGCTTTCCAGGTGGTAGTAGTCAAGGTTGACGCGGGTCGGCGTGCCCAGGc                                             >  2:102521/1‑148 (MQ=255)
                                                                                                              catcatTCGGCTTGCTCGGGTGTGCCGAGGTGCGCCCGGCGCTGCCGTTGGTCGGGATGGTGTAAGGGATGCCCGAATCCGGCAGGTCGTCGCTTTCCAGGTGGTAGTAGTCAAGGTTGACGCGGGTCGGCGTGCCCAGGCCAAAg                                        <  1:248970/146‑1 (MQ=255)
                                                                                                              catcatTCGGCTTGCTCGGGTGTGCCGAGGTGCGCCCGGCGCTGCCGTTGGTCGGGATGGTGTAAGGGATGCCCGAATCCGGCAGGTCGTCGATTTCCAGGTGGTAGTAGTCAAGGTTGACTCGGGTCCGAGTGCCCAGGCCAAAg                                        >  2:248970/1‑146 (MQ=255)
                                                                                                               atcatTCGGCTTGCTCGGGTGTGCCGAGGTGCGCCCGGCGCTGCCGTTGGTCGGGATGGTGTAAGGGATGCCCGAATCCGGCAGGTCGTCGCTTTCCAGGTGGTAGTAGTCAAGGTTGACGCGGGTCGGCGTGCCCAGGCCAAAGGCCa                                    <  2:240370/149‑1 (MQ=255)
                                                                                                                tcatTCGGCTTGCTCGGGTGTGCCGAGGTGCGCCCGGCGCTGCAGTTGGTCGGGATGGTGTAAGGGATGCCCGAATCCGGCAGGTCGTCGCTTTCCAGGTGGTAGTAGTCAAGGTTGACGCGGGTCGGCGTGCCCAGGCCAACGGCCAg                                   >  4:328659/1‑149 (MQ=255)
                                                                                                                tcatTCGGCTTGCTCGGGTGTGCCGAGGTGCGCCCGGCGCCGCCGTTGGTCGGGATGGTGTAAGGGATGCCCGAATCCGGCAGGTCGTCGCTTTCCAGGTGGTAGTAGTCAAGGTTGACGCGGGTCGGCGTGCCCAGGCCAAAGGCCAg                                   <  4:307483/149‑1 (MQ=255)
                                                                                                                   ttCGGCTTGCTCGGGTGTGCCGAGGTGCGCCCGGCGCTGCCGTTGGTCGGGATGGTGTAAGGGATGCCCGAATCCGGCAGGTCGTCGCTTTCCAGGTGGTAGTAGTCAAGGTTGACGCGGGTCGGCGTGCCCAGGCCAAAGGCCAGCGa                                >  4:50492/1‑149 (MQ=255)
                                                                                                                        cTTGCTCGGGTGTGCCGAGGTGCGCCCGGCGCTGCCGTTGGTCGGGATGGTGTAAGGGATGCCCGAATCCGGCAGGTCGTCGCTTTCCAGGTGGTAGTAGTCAAGGTTGACGCGGGTCGGCGTGCCCAGGCCAAAGGCCAGCGACgggg                           <  6:323121/149‑1 (MQ=255)
                                                                                                                                     gCCGAGGTGCGCCCGGCGCTGCCGTTGGTCGGGATGGTGTAAGGGATGCCCGAATCCGGCAGGTCGTCGCTTTCCAGGTGGTAGTAGTCAAGGTTGACGCGGGTCGGCGTGCCCAGGCCaaa                                         >  7:333209/1‑122 (MQ=255)
                                                                                                                                     gCCGAGGTGCGCCCGGCGCTGCCGTTGGTCGGGATGGTGTAAGGGATGCCCGAATCCGGCAGGTCGTCGCTTTCCAGGTGGTAGTAGTCAAGGTTGACGCGGGTCGGCGTGCCCAGGCCaaa                                         <  8:333209/122‑1 (MQ=255)
                                                                                                                                      ccGAGGTGCGCCCGGCGCTGCCGTTGGTCGGGATGGTGTAAGGGATGCCCGAATCCGGCAGGTCGTCGCTTTCCAGGTGGTAGTAGTCAAGGTTGACGCGGGTCGGCGTGCCCAGGCCAAAGGCCAGCGACGGGGCGACACCCCAGCgg             >  8:338678/1‑149 (MQ=255)
                                                                                                                                       cGAGGTGCGCCTGGCGCTGCCGTTGGTCGGGATGGTGTAAGGGATGCCCGAATCCGGCAGGTCGTCGCTTTCCAGGTGGTAGTAGTCAAGGTTGACGCGGGTCGGCGTGCCCAGGCCAAAGGCCAGCGACgggg                           >  3:158580/1‑134 (MQ=255)
                                                                                                                                       cGAGGTGCGCCTGGCGCTGCCGTTGGTCGGGATGGTGTAAGGGATGCCCGAATCCGGCAGGTCGTCGCTTTCCAGGTGGTAGTAGTCAAGGTTGACGCGGGTCGGCGTGCCCAGGCCAAAGGCCAGCGACgggg                           >  3:164349/1‑134 (MQ=255)
                                                                                                                                       cGAGGTGCGCCTGGCGCTGCCGTTGGTCGGGATGGTGTAAGGGATGCCCGAATCCGGCAGGTCGTCGCTTTCCAGGTGGTAGTAGTCAAGGTTGACGCGGGTCGGCGTGCCCAGGCCAAAGGCCAGCGACgggg                           <  4:164349/134‑1 (MQ=255)
                                                                                                                                       cGAGGTGCGCCTGGCGCTGCCGTTGGTCGGGATGGTGTAAGGGATGCCCGAATCCGGCAGGTCGTCGCTTTCCAGGTGGTAGTAGTCAAGGTTGACGCGGGTCGGCGTGCCCAGGCCAAAGGCCAGCGACgggg                           <  4:158580/134‑1 (MQ=255)
                                                                                                                                                 ccGGCGCTGCCGTTGGTCGGGATGGTGTAAGGGATGCCCGAATCCGGCAGGTCGTCGCTTTCCAGGTGGTAGTAGTCAAGGTTGACGCGGGTCGGCGTGCCCAGGCCAAAGGCCAGCGACGGGGCGATACCCCAGCGGTCGTAGTTGAc  <  1:102521/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GATGGTCAGCGAGTCGGTCAGGTCGTGCTCGACGGCGAAGGTTGCGATATCCACTCGGGTCTTGCGGAAGTCGCGGCCGGTCAGGCCGTAGAAGTTGTCACTGTCGCCGCCATCATTCGGCTTGCTCGGGTGTGCCGAGGTGCGCCCGGCGCTGCCGTTGGTCGGGATGGTGTAAGGGATGCCCGAATCCGGCAGGTCGTCGCTTTCCAGGTGGTAGTAGTCAAGGTTGACGCGGGTCGGCGTGCCCAGGCCAAAGGCCAGCGACGGGGCGATACCCCAGCGGTCGTAGTTGAC  >  NC_002947/999141‑999434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑