Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,025,784 T→G 5.8% D395A (GAC→GCC)  dppA‑II ← dipeptide ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,025,7840TG 5.8% 257.0 / 5.1 81D395A (GAC→GCC) dppA‑IIdipeptide ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (42/34);  new base G (2/3);  total (44/37)
Fisher's exact test for biased strand distribution p-value = 6.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

AGGGTACCCAGCCAGTTGTCCGGGTCGCCGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTCCGGCGCGTACGGGGCGTCCTT  >  NC_002947/1025640‑1025932
                                                                                                                                                |                                                                                                                                                    
agGGTACCCAGCCAGTTGTCCGGGTCGCCGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGc                                                                                                                                                  <  8:202165/149‑1 (MQ=255)
  ggTACCCAGCCAGTTGTCCGGGTCGCCGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCt                                                                                                                                                >  5:299898/1‑149 (MQ=255)
     aCCCAGCCAGTTGTCCGGGTCGCCGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTg                                                                                                                                               >  3:120734/1‑147 (MQ=255)
      cccAGCCAGTTGTCCGGGTCGCCGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGa                                                                                                                                             <  4:120734/148‑1 (MQ=255)
      cccAGCCAGTTGTCCGGGTCGCCGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGa                                                                                                                                             <  2:65066/148‑1 (MQ=255)
          gccagTTGTCCGGGTCGCCGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGGCGGACTGGAGCaag                                                                                                                                        <  2:42863/149‑3 (MQ=255)
           ccagTTGTCCGGGTCGCCGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGGCGGACTGGAGCaagc                                                                                                                                       <  5:160592/149‑4 (MQ=255)
                   cctggTCGCCGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCAt                                                                                                                                <  8:197148/145‑1 (MQ=255)
                            cGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCATGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCa                                                                                                                      >  1:303355/1‑149 (MQ=255)
                                   aCCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGt                                                                                                               >  6:8312/1‑149 (MQ=255)
                                   aCCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGt                                                                                                               >  7:345557/1‑149 (MQ=255)
                                   aCCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGt                                                                                                               >  8:17195/1‑149 (MQ=255)
                                         ccAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGcc                                                                                                                                  <  7:129420/124‑1 (MQ=255)
                                         ccAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGcc                                                                                                                                  >  8:129420/1‑124 (MQ=255)
                                          cAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACgg                                                                                                        <  5:146005/149‑1 (MQ=255)
                                           aGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCg                                                                                                                                     <  6:256049/119‑1 (MQ=255)
                                           aGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCg                                                                                                                                     >  5:256049/1‑119 (MQ=255)
                                            gCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGc                                                                                                      >  2:254677/1‑149 (MQ=255)
                                                 tCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTg                                                                                                  >  2:322464/1‑148 (MQ=255)
                                                     ggCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAg                                                                                                                             >  5:218714/1‑117 (MQ=255)
                                                     ggCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAg                                                                                                                             <  6:218714/117‑1 (MQ=255)
                                                     ggCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTGCGGCCATCAGCGTGGCATGGGGGTTGTACGGGCGTTGCACCg                                                                                             >  4:311030/1‑149 (MQ=255)
                                                       cGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGc                                                                                           <  1:322464/149‑1 (MQ=255)
                                                              gTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGCCCATCAGCTTGGCATTGGGGTTGTACGGCCGTTGCACCGGCATGGcc                                                                                     >  4:248470/1‑148 (MQ=255)
                                                                 cTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCcac                                                                                  <  7:17195/148‑1 (MQ=255)
                                                                   cACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAgg                                                                               >  6:164844/1‑149 (MQ=255)
                                                                    aCCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAggg                                                                              >  6:251314/1‑149 (MQ=255)
                                                                    aCCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAggg                                                                              >  7:259754/1‑149 (MQ=255)
                                                                    aCCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAggg                                                                              >  8:88881/1‑149 (MQ=255)
                                                                      cGTTTTTCATGCGCTTGAGGTATTCGGCCCATTCTTAGCTGACGATGCGCACGTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTg                                                                            <  3:273233/149‑1 (MQ=255)
                                                                              aTGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGTGTTGCACCGGCATGGCCCACAGGGTGATCTCGGt                                                                    <  8:198442/149‑1 (MQ=255)
                                                                                  gcTTGAGGTATTCCCCCCTTTCGTAGCTGCCGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGAATTGGGGTTGTAGGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCa                                                                <  3:311030/149‑1 (MQ=255)
                                                                                    ttGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAg                                                                                >  8:243940/1‑131 (MQ=255)
                                                                                    ttGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAg                                                                                <  7:243940/131‑1 (MQ=255)
                                                                                      gAGGCATTCGCCCCATTCGTACCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGGCCGCCTGGAGCATTTCGGCCAGCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAgg                                                              <  8:141095/147‑1 (MQ=255)
                                                                                        ggTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAg                                                               <  5:226251/144‑1 (MQ=255)
                                                                                        ggTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAg                                                               >  6:226251/1‑144 (MQ=255)
                                                                                         gTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGa                                                         <  5:251314/149‑1 (MQ=255)
                                                                                         gTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGa                                                         >  3:22566/1‑149 (MQ=255)
                                                                                           aTTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGaca                                                       >  2:300228/1‑149 (MQ=255)
                                                                                               gCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACAccc                                                    <  2:303355/148‑1 (MQ=255)
                                                                                               gCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCg                                                   >  5:61701/1‑149 (MQ=255)
                                                                                                ccccATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGc                                                  >  3:315105/1‑149 (MQ=255)
                                                                                                  ccATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTAc                                                                                                          <  4:354428/91‑1 (MQ=255)
                                                                                                  ccATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTAc                                                                                                          >  3:354428/1‑91 (MQ=255)
                                                                                                  ccATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCct                                                >  1:119130/1‑149 (MQ=255)
                                                                                                  ccATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCct                                                >  5:218587/1‑149 (MQ=255)
                                                                                                  ccATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCct                                                >  4:54108/1‑149 (MQ=255)
                                                                                                  ccATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCct                                                <  7:88881/149‑1 (MQ=255)
                                                                                                   cATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGc                                                                                                 >  7:91080/1‑99 (MQ=255)
                                                                                                   cATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGc                                                                                                 <  8:91080/99‑1 (MQ=255)
                                                                                                   cATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCctg                                               >  3:202572/1‑149 (MQ=255)
                                                                                                    aTTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCctgc                                              <  6:218587/149‑1 (MQ=255)
                                                                                                         tAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCGATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGttt                                                           <  7:322834/131‑1 (MQ=255)
                                                                                                         tAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGttt                                                           >  8:322834/1‑131 (MQ=255)
                                                                                                          aGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGc                                                  <  5:222096/139‑1 (MQ=255)
                                                                                                          aGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGc                                                  >  6:222096/1‑139 (MQ=255)
                                                                                                           gCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCa                                       <  6:299898/149‑1 (MQ=255)
                                                                                                                     cgcACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGc                             <  8:345557/149‑1 (MQ=255)
                                                                                                                     cgcACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAACTGTCTGGc                             <  6:61701/149‑1 (MQ=255)
                                                                                                                      gcACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGcc                            >  2:1664/1‑149 (MQ=255)
                                                                                                                        aCCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACAccc                                                    >  8:348802/1‑123 (MQ=255)
                                                                                                                        aCCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACAccc                                                    <  7:348802/123‑1 (MQ=255)
                                                                                                                          cTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCtttt                        >  6:189067/1‑149 (MQ=255)
                                                                                                                          cTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCttt                         >  1:257900/1‑148 (MQ=255)
                                                                                                                          cTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCttt                         <  2:257900/148‑1 (MQ=255)
                                                                                                                              aaTCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTCCg                     <  8:259754/148‑1 (MQ=255)
                                                                                                                              aaTCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTCCg                     <  4:315105/148‑1 (MQ=255)
                                                                                                                                tCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACgg                                                                                                        <  5:269237/63‑1 (MQ=255)
                                                                                                                                tCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACgg                                                                                                        >  6:269237/1‑63 (MQ=255)
                                                                                                                                tCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTCCGgcg                  >  4:88360/1‑149 (MQ=255)
                                                                                                                                tCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTCCGgcg                  >  8:274598/1‑149 (MQ=255)
                                                                                                                                  cGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTCCgg                    <  3:316746/145‑1 (MQ=255)
                                                                                                                                  cGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTCCgg                    >  4:316746/1‑145 (MQ=255)
                                                                                                                                   gAGCTTGCTCCAGTCCGCCTGGAGCATTTGGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTCCGGcgcg                >  1:26821/1‑148 (MQ=255)
                                                                                                                                   cgaaatGCTCCAGGCGGACTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTCCGGcgcg                >  3:300364/6‑148 (MQ=255)
                                                                                                                                     aaatGCTCCAGGCGGACTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTCCGGCGCGTAc             >  6:327975/4‑149 (MQ=255)
                                                                                                                                         gCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTCCGGCGCGTACgggg         >  3:131295/1‑149 (MQ=255)
                                                                                                                                         gCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTCCGGCGCGTACgggg         >  7:316379/1‑149 (MQ=255)
                                                                                                                                         gCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTCCGGCGCGTACggg          <  5:164844/148‑1 (MQ=255)
                                                                                                                                          cTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTCCGGCGCGTACGGGGc        <  1:254677/149‑1 (MQ=255)
                                                                                                                                                tCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTCCGGCGCGTACGGGGCGTCCtt  <  4:131295/149‑1 (MQ=255)
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AGGGTACCCAGCCAGTTGTCCGGGTCGCCGTTGTCACCGGTCCAGCCGATCAGGGCGATGTCGTGCTCACCGTTTTTCATGCGCTTGAGGTATTCGCCCCATTCGTAGCTGACGATGCGCACCTTGAATCCGAGCTTGCTCCAGTCCGCCTGGAGCATTTCGGCCATCAGCTTGGCATTGGGGTTGTACGGGCGTTGCACCGGCATGGCCCACAGGGTGATCTCGGTGCCAGGTTTGACACCCGCCTGCTGGATCAGCTGTCTGGCCTTTTCCGGCGCGTACGGGGCGTCCTT  >  NC_002947/1025640‑1025932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑