Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,164,075 G→A 5.9% V128I (GTC→ATC)  yeaD → glucose‑6‑phosphate 1‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,164,0750GA 5.9% 202.0 / 2.0 68V128I (GTC→ATC) yeaDglucose‑6‑phosphate 1‑epimerase
Reads supporting (aligned to +/- strand):  ref base G (27/36);  new base A (2/2);  total (30/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.90e-01

GTGCTGGCCCTGGTATGGCCGTCATCCCAGCGAAGACCTGTGGCCGGCCCACGGTTGGGCGCGGTTGCTGGACTGGAAGCTGGTGGACAGCCGTGAGGACGAGGAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGCTGAGCCTGAGCACCGAACATCAGGACAGCGAACCCTGCCAGTTGAGCCATGCGCTGCTGGCGTACTGGCGCATCAGTGACGTGTCCGAGATAGCGCTGTCTGGTCTGG  >  NC_002947/1163927‑1164219
                                                                                                                                                    |                                                                                                                                                
gtgCTGGCCCTGGTATGGCCGTCATCCCAGCGAAGACCTGTGGCCGGCCCACGGTTGGGCGCGGTTGCTGGACTGGAAGCTGGTGGACAGCCGTGAGGACGAGGAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAgg                                                                                                                                                  <  7:331309/149‑1 (MQ=255)
   cTGGCCCTGGTATGGCCGTCATCCCAGCGAAGACCTGTGGCCGGCCCACGGTTGGGCGCGGTTGCTGGACTGGAAGCTGGTGGACAGCCGTGAGGACGAGGAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTc                                                                                                                                                <  1:83690/148‑1 (MQ=255)
   cTGGCCCTGGTATGGCCGTCATCCCAGCGAAGACCTGTGGCCGGCCCACGGTTGGGCGCGGTTGCTGGACTGGAAGCTGGTGGACAGCCGTGAGGACGAGGAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTc                                                                                                                                                <  7:217329/148‑1 (MQ=255)
            gTATGGCCGTCATCCCAGCGAAGACCTGTGGCCGGCCCACGGTTGGGCGCGGTTGCTGGACTGGAAGCTGGTGGACAGCCGTGAGGACGAGGAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATg                                                                                                                                      >  6:21995/1‑149 (MQ=255)
              aTGGCCGTCATCCCAGCGAAGACCTGTGGCCGGCCCACGGTTGGGCGCGGTTGCTGGACTGGAAGCTGGTGGGCAGCCGTGAGGACGAGGAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGcc                                                                                                                                    >  3:58796/1‑149 (MQ=255)
                  ccGTCATCCCAGCGAAGACCTGTGGCCGGCCCACGGTTGGGCGCGGTTGCTGGACTGGAAGCTGGTGGACAGCCGTGAGGACGAGGAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGc                                                                                                                                >  8:49480/1‑149 (MQ=255)
                     tCATCCCATCGAAGACCTGTGTCCGGCCCACGGTTGGGCGCGGTTGCTGGACTGGAAGCTGGTGGACAGCCGTGAGGACGAGGAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTgg                                                                                                                             <  2:116128/149‑1 (MQ=255)
                     tCATCCCAGCGAAGACCTGTGGCCGGCCCACGGTTGGGCGCGGTTGCTGGACTGGAAGCTGGTGGACAGCCGTGAGGACGAGGAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTgg                                                                                                                             <  6:158949/149‑1 (MQ=255)
                     tCATCCCAGCGAAGACCTGTGGCCGGCCCACGGTTGGGCGCGGTTGCTGGACTGGAAGCTGGTGGACAGCCGTGAGGACGAGGAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTgg                                                                                                                             <  4:58796/149‑1 (MQ=255)
                       aTCCCAGCGAAGACCTGTGGCCGGCCCACGGTTGGGCGCGGTTGCTGGACTGGAAGCTGGTGGACAGCCGTGAGGACGAGGAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTggg                                                                                                                            >  2:173811/1‑148 (MQ=255)
                        tCCCAGCGAAGACCTGTGGCCGGCCCACGGTTGGGCGCGGTTGCTGGACTGGAAGCTGGTGGACAGCCGTGAGGACGAGGAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTggg                                                                                                                            >  6:56676/1‑147 (MQ=255)
                          ccAGCGAAGACCTGTGGCCGGCCCACGGTTGGGCGCGGTTGCTGGACTGGAAGCTGGTGGACAGCCGTGAGGACGAGGAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGc                                                                                                                        >  7:17358/1‑149 (MQ=255)
                                     cTGTGGCCGGCCCACGGTTGGGCGCGGTTGCTGGACTGGAAGCTGGTGGACAGCCGTGAGGACGAGGAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGct                                                                                                             <  7:49480/149‑1 (MQ=255)
                                      tgtgGCCGGCCCACGGTTGGGCGCGGTTGCTGGACTGGAAGCTGGTGGACAGCCGTGAGGACGAGGAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGct                                                                                                             >  3:212062/1‑148 (MQ=255)
                                        tgGCGGGCGCTCGGTTGGGCGCGGTTGGTGGACTGGAAGCTGGTGGACAGCCGTGAGGACGAGGAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCATGTCGACCTGCATGCCCGGCTGGGTAGGCGCATGGAGctga                                                                                                           <  4:97953/148‑1 (MQ=255)
                                        tgGCCGGCCCACGGTTGGGCGCGGTTGCTGGACTGGAAGCTGGTGGACAGCCGTGAGGACGAGGAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGctga                                                                                                           <  3:89222/148‑1 (MQ=255)
                                        tgGCCGGCCCACGGTTGGGCGCGGTTGCTGGACTGGAAGCTGGTGGACAGCCGTGAGGACGAGGAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGctga                                                                                                           <  1:173811/148‑1 (MQ=255)
                                             ggccCACGGTTGGGCGCGGTTGCTGGACTGGAAGCTGGTGGACAGCCGTGAGGACGAGGAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGctgagcctga                                                                                                     <  3:57956/149‑1 (MQ=255)
                                                  aCGGTTGGGCGCGGTTGCTGGACTGGAAGCTGGTGGACAGCCGTGAGGACGAGGAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGctgagcc                                                                                                        <  3:214281/141‑1 (MQ=255)
                                                  aCGGTTGGGCGCGGTTGCTGGACTGGAAGCTGGTGGACAGCCGTGAGGACGAGGAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGctgagcc                                                                                                        >  4:214281/1‑141 (MQ=255)
                                                          gcgcGGTTGCTGGACTGGAAGCTGGTGGACAGCCGTGAGGACGAGGAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGCTGAGCCTGAGCACCGAACATCa                                                                                        <  5:158731/149‑1 (MQ=255)
                                                                    tggactggaAGCTGGTGGACAGCCGTGAGGACGAGGAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGCTGAGCCTGAGCACCGAACATCAg                                                                                       <  7:210351/140‑1 (MQ=255)
                                                                    tggactggaAGCTGGTGGACAGCCGTGAGGACGAGGAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGCTGAGCCTGAGCACCGAACATCAg                                                                                       >  8:210351/1‑140 (MQ=255)
                                                                    tggacagcaACCTCGTGGAAACCCGAGACCACGAGGAGCGCGTGACCCACAAATGGCCGCTGGACATGTGCCACTTCCAGCTCGACCTGCAAGCCCCGCAGGGAAGCCGCATGGAGCTGAGCCAGAGCACCCAACATCACGACAGCGa                                                                               >  4:149269/1‑148 (MQ=255)
                                                                     ggactggaAGCTGGTGGACAGCCGTGAGGACGAGGAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGCTGAGCCTGAGCACCGAACATCAGGACAGCGAAc                                                                             >  2:65682/1‑149 (MQ=255)
                                                                         tggaAGCTGGTGGACAGCCGTGAGGACGAGGAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGCTGAGCCTGAGCACCGAACATCa                                                                                        >  1:185250/1‑134 (MQ=255)
                                                                         tggaAGCTGGTGGACAGCCGTGAGGACGAGGAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGCTGAGCCTGAGCACCGAACATCa                                                                                        <  2:185250/134‑1 (MQ=255)
                                                                           gaAGCTGGTGGACAGCCGTGAGGACTAGTAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGATCGACCTGc                                                                                                                                         <  6:167095/83‑1 (MQ=255)
                                                                           gaAGCTGGTGGACAGCCGTGAGGACGAGGAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGCTGAGCCTGAGCACCGAACATCAGGa                                                                                     <  4:53971/135‑1 (MQ=255)
                                                                           gaAGCTGGTGGACAGCCGTGAGGACGAGGAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGCTGAGCCTGAGCACCGAACATCAGGa                                                                                     >  3:53971/1‑135 (MQ=255)
                                                                           gaAGCTGGTGGACAGCCGTGAGGACGAGGAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGCTGAGCCTGAGCACCGAACATCAGGACAGCGAACCCTGc                                                                        >  6:83006/1‑148 (MQ=255)
                                                                           gaAGCTGGTGGACAGCCGTGAGGACGAGGAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGCTGAGCCTGAGCACCGAACATCAGGACAGCGAACCCTGc                                                                        >  8:57413/1‑148 (MQ=255)
                                                                           caaGCTGGTGGACAGCCGTGAGGACGAGGAGCGCGTGAACCTGAAATGGCGGCTCGACCTGTGCGACTGGCAGATCGACCTGc                                                                                                                                         >  5:167095/2‑83 (MQ=255)
                                                                            aaGCTGGTGGATAGCCGTGAGGACGAGGAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGATCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGCTGAGCCTGAGCACCGAACATCAGGa                                                                                     <  1:216861/134‑1 (MQ=255)
                                                                            aaGCTGGTGGACAGCCGTGAGGACGAGGAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGATCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGCTGAGCCTGAGCACCGAACATCAGGa                                                                                     >  2:216861/1‑134 (MQ=255)
                                                                              gCTGGTGGACAGCCGTGAGGACGAGGAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGCTGAGCCTGAGCACCGAACATCAGGACAGCGAACCCTGc                                                                        <  6:226519/145‑1 (MQ=255)
                                                                              gCTGGTGGACAGCCGTGAGGACGAGGAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGCTGAGCCTGAGCACCGAACATCAGGACAGCGAACCCTGc                                                                        >  5:226519/1‑145 (MQ=255)
                                                                                 ggtggACAGCCGTGAGGACGAGGAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGCTGAGCCTGAGCACCGAACATCAGGACAGCGAACCCTGCCAGtt                                                                   >  4:339333/1‑147 (MQ=255)
                                                                                 ggtggACAGCCGTGAGGACGAGGAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGCTGAGCCTGAGCACCGAACATCAGGACAGCGAACCCTGCCAGtt                                                                   >  3:91333/1‑147 (MQ=255)
                                                                                  gtggACAGCCGTGAGGACGAGGAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGCTGAGCCTGAGCACCGAACATCAGGACAGCGAACCCTGCCAGTTGAg                                                                <  1:204415/149‑1 (MQ=255)
                                                                                     gACAGCCGTGAGGACGAGGAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGCTGAGCCTGAGCACCGAACATCAGGACAGCGAACCCTGCCAGTTGAGcc                                                              >  4:55589/1‑148 (MQ=255)
                                                                                       cAGCCGTGAGGACGAGGAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGCTGGGCCTGAGCACCGAACATCAGGACAGCGAACCCTGCCAGTTGAGCCATg                                                           <  4:6208/149‑1 (MQ=255)
                                                                                       cAGCCGTGAGGACGAGGAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGCTGAGCCTGAGCACCGAACATAAGGACAGCGAACCCTGCCAGTTGAGCCATg                                                           >  7:137097/1‑149 (MQ=255)
                                                                                            gTGAGGACGAGGAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGCTGAGCCTGAGCACCGAACATCAGGACAGCGAACCCTGCCAGTTGAGCCATGCgctg                                                      <  5:21995/149‑1 (MQ=255)
                                                                                               aGGACGAGGAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGCTGAGCCTGAGCACCGAACATCAGGACAGCGAACCCTGCCAGTTGAGCCATGCGctgctg                                                   <  4:91333/149‑1 (MQ=255)
                                                                                               aGGACGAGGAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGCTGAGCCTGAGCACCGAACATCAGGACAGCGAACCCTGCCAGTTGAGCCATGCGctgctg                                                   <  3:55589/149‑1 (MQ=255)
                                                                                                  aCGAGGAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGCTGAGCCTGAGCACCGAACATCAGGACAGCGAACCCTGCCAGTTGAGCCATGCGCTGCTGGCg                                                <  1:65682/149‑1 (MQ=255)
                                                                                                    gaggagGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGCTGAGCCTGAGCACCGAACATCAGGACAGCGAACCCTGCCAGTTGAGCCATGCGCTGCTGGCGTa                                              <  5:56676/149‑1 (MQ=255)
                                                                                                       gaggGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGctgagcctga                                                                                                     <  3:106265/91‑1 (MQ=255)
                                                                                                       gaggGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGctgagcctga                                                                                                     >  4:106265/1‑91 (MQ=255)
                                                                                                       gaggGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGCTGAGCCTGAGCACCGAACATCAGGACAGCGAACCCTGCCAGTTGAGCCATGCGCTGCTGGCGTACTg                                           >  1:191189/1‑149 (MQ=255)
                                                                                                         gggCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGCTGAGCCTGAGCACCGAACATCAGGACAGCGAACCCTGCCAGTTGAGCCATGCGCTGCTGGCGTACTGgc                                         <  5:83006/149‑1 (MQ=255)
                                                                                                           gCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGCTGAGCCTGAGCACCGAACATCAGGACAGCGAACCCTGCCAGTTGAGCCATGCGCTGCTGGCGTACTGgc                                         >  6:56331/1‑147 (MQ=255)
                                                                                                           gCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGCTGAGCCTGAGCACCGAACATCAGGACAGCGAACCCTGCCAGTTGAGCCATGCGCTGCTGGCGTACTGgc                                         <  5:56331/147‑1 (MQ=255)
                                                                                                               gACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGCTGAGCCTGAGCACCGAACATCAGGACAGCGAACCCTGCCAGTTGAGCCATGCGCTGCTGGCGTACTGGCGCAt                                     >  6:352973/1‑147 (MQ=255)
                                                                                                                 cccTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGCTGAGCCTGAGCACCGAACATCAGGACAGCg                                                                                >  3:345354/1‑102 (MQ=255)
                                                                                                                 cccTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGCTGAGCCTGAGCACCGAACATCAGGACAGCg                                                                                <  4:345354/102‑1 (MQ=255)
                                                                                                                  ccTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCGGGGTAGCCGCATGGAGCTGAGCCTGAGCACCGAACATCAGGACAGCGAACCCTGCCAGTTGAGCCATGCGCTGCTGGCGTACTGGCGCATCAGTg                                <  2:48523/149‑1 (MQ=255)
                                                                                                                      aaaTGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGCTGAGCCTGAGCACCGAACATCAGGACAGCGAACCCTGCCAGTTGAGCCATGCGCTGCTGCCGTACTGGCGCATCAGTGACgt                            <  7:57413/149‑1 (MQ=255)
                                                                                                                            cggcTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGCTGAGCCTGAGCACCGAACATCAGGACAGCGAACCCTGCCAGTTGAGCCATGCGCTGCTGGCGTACTGGCGCATCAGTGACGTGTCCga                      <  4:212062/149‑1 (MQ=255)
                                                                                                                                  gACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGCTGAGCCTGAGCACCGAACATCAGGACAGCGAACCCTGCCAGTTGAGCCATGCGCTGCTGGCGTACTGGCGCATCAGTGACGTGTCCGAGATAg                 <  5:352973/148‑1 (MQ=255)
                                                                                                                                  gACCTGTGCGACTGGCAGGCCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGCTGAGCCTGAGCACCGAACATCAGGACAGCGAACCCTGCCAGTTGAGCCATGCGCTGCTGGCGTACTGGCGCATCAGTGACGTGTCCGAGATAgc                >  8:179224/1‑149 (MQ=255)
                                                                                                                                   aCCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGCTGAGCCTGAGCACCGAACATCAGGACAGCGAACCCTGCCAGTTGAGCCATGCGCTGCTGGCGTACTGGCGCATCAGTGACGTGTCCGAGATAgcg               <  8:17358/149‑1 (MQ=255)
                                                                                                                                     cTGGGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGCTGAGCCTGAGCACCGAACATCAGGACAGCGAACCCTGCCAGTTGAGCCATGCGCTGCTGGCGTACTGGCGCATCAGTGACGTGTCCGAGATAGCGCt             <  3:149269/149‑1 (MQ=255)
                                                                                                                                          cGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGCTGAGCCTGAGCACCGAACATCAGGACAGCGAACCCTGCCAGTTGAGCCATGCGCTGCTGGCGTACTGGCGCATCAGTGACGTGTCCGAGATAGCGCTgtctg        <  4:160510/149‑1 (MQ=255)
                                                                                                                                          cGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGCTGAGCCTGAGCACCGAACATCAGGACAGCGAACCCTGCCAGTTGAGCCATGCGCTGCTGGCGTACTGGCGCATCAGTGACGTGTCCGAGATAGCGCTgtctg        >  3:160510/1‑149 (MQ=255)
                                                                                                                                          cGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGCTGAGCCTGAGCACCGAACATCAGGACAGCGAACCCTGCCAGTTGAGCCATGCGCTGCTGGCGTACTGGCGCATCAGTGACGTGTCCGAGATAGCGCTgtct         <  8:137097/148‑1 (MQ=255)
                                                                                                                                                gCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGCTGAGCCTGAGCACCGAACATCAGGACAGCGAACCCTGCCAGTTGAGCCATGCGCTGCTGGCGTACTGGCGCATCAGTGACGTGTCCGAGATAGCGCTgtctggtctg   >  8:85693/1‑148 (MQ=255)
                                                                                                                                                gCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGCTGAGCCTGAGCACCGAACATCAGGACAGCGAACCCTGCCAGTTGAGCCATGCGCTGCTGGCGTACTGGCGCATCAGTGACGTGTCCGAGATAGCGCTGtctggtctgg  <  5:362859/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
GTGCTGGCCCTGGTATGGCCGTCATCCCAGCGAAGACCTGTGGCCGGCCCACGGTTGGGCGCGGTTGCTGGACTGGAAGCTGGTGGACAGCCGTGAGGACGAGGAGGGCGTGACCCTGAAATGGCGGCTGGACCTGTGCGACTGGCAGGTCGACCTGCATGCCCGGCTGGGTAGCCGCATGGAGCTGAGCCTGAGCACCGAACATCAGGACAGCGAACCCTGCCAGTTGAGCCATGCGCTGCTGGCGTACTGGCGCATCAGTGACGTGTCCGAGATAGCGCTGTCTGGTCTGG  >  NC_002947/1163927‑1164219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑