Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,232,737	A→G	7.7%	intergenic (‑25/+192)	glpD ← / ← glpR	aerobic glycerol‑3‑phosphate dehydrogenase/DNA‑binding transcriptional repressor GlpR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,232,737	0	A	G	7.7%	147.7 / 2.7	52	intergenic (‑25/+192)	glpD/glpR	aerobic glycerol‑3‑phosphate dehydrogenase/DNA‑binding transcriptional repressor GlpR
Reads supporting (aligned to +/- strand):  ref base A (23/25);  new base G (2/2);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTTCAAGCCGCGACCAGCGGCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAGGGGGTACGGCTTGGGTGAGGGGGCGC  >  NC_002947/1232590‑1232881                                                                                                                                                    |                                                                                                                                                 cTTCAAGCCGCGACCAGCGGCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACATCGaa                                                                                                                                                 <  8:276655/149‑1 (MQ=255)    cAAGCCGCGACCAGCGGCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACATCGAAt                                                                                                                                                <  2:77313/147‑1 (MQ=255)         cgcgACCAGCGGCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACa                                                                                                                                         >  3:90966/1‑149 (MQ=255)             aCCAGCGGCGTCGGCAGCGATGCCCCAGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGTGCTGGGACGAATCTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGa                                                                                                                                     <  5:108284/149‑1 (MQ=255)              ccAGCGGCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGAt                                                                                                                                    <  7:201053/149‑1 (MQ=255)                 gcggcgTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGtt                                                                                                                                 <  8:194814/149‑1 (MQ=255)                  cggcgTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTc                                                                                                                                <  4:267138/149‑1 (MQ=255)                    gcgTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGt                                                                                                                              <  1:288299/149‑1 (MQ=255)                    gcgTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGt                                                                                                                              <  4:90966/149‑1 (MQ=255)                     cgTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGtt                                                                                                                             <  4:82496/149‑1 (MQ=255)                       tCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTg                                                                                                                           <  7:43182/149‑1 (MQ=255)                                    ccACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAgc                                                                                                              >  2:123660/1‑149 (MQ=255)                                     cACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAatat                                                                                                                  >  6:64042/1‑144 (MQ=255)                                     cACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAatat                                                                                                                  <  5:64042/144‑1 (MQ=255)                                                      cgccgATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTAGCGAAAATATTAGCGCAACAGAACGACGGTct                                                                                            >  1:104085/1‑148 (MQ=255)                                                                      gTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCtata                                                                            <  5:115415/149‑1 (MQ=255)                                                                      gTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCtata                                                                            >  3:200421/1‑149 (MQ=255)                                                                      gTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCta                                                                              <  4:200421/147‑1 (MQ=255)                                                                              aGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAAc                                                                    <  1:120606/149‑1 (MQ=255)                                                                                   gCTGTTGGCGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATc                                                               <  5:44069/149‑1 (MQ=255)                                                                                      gTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGa                                                                 >  8:121893/1‑144 (MQ=255)                                                                                      gTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGa                                                                 <  7:121893/144‑1 (MQ=255)                                                                                           gggCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGAc                                                        >  6:73592/1‑148 (MQ=255)                                                                                                  gACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGt                                                >  5:37893/1‑149 (MQ=255)                                                                                                            gctggGACACGGGCAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCtata                                                                            >  1:317023/1‑111 (MQ=255)                                                                                                            gctggGACACGGGCAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCtata                                                                            <  2:317023/111‑1 (MQ=255)                                                                                                              tggGACACGGGCAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACg                                     >  1:169137/1‑148 (MQ=255)                                                                                                                ggACACGGGCAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGt                                                <  2:7547/135‑1 (MQ=255)                                                                                                                ggACACGGGCAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGt                                                >  1:7547/1‑135 (MQ=255)                                                                                                                 gACACGGGCAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGaaaa                                 >  2:262084/1‑149 (MQ=255)                                                                                                                    acGGGCAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAAtt                               >  8:326884/1‑148 (MQ=255)                                                                                                                    acGGGCAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATACAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTc                              <  1:262084/149‑1 (MQ=255)                                                                                                                      gggCAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAg                            >  2:224970/1‑149 (MQ=255)                                                                                                                       ggCAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAgg                           <  1:224970/149‑1 (MQ=255)                                                                                                                       ggCAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAgg                           <  7:326884/149‑1 (MQ=255)                                                                                                                       ggCAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAAGGAAGTGAACATTTACGAAAATTCAgg                           >  6:109533/1‑149 (MQ=255)                                                                                                                        gCAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACTAAAATTCAgcg                          >  2:91480/1‑147 (MQ=255)                                                                                                                         cAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAgggg                         <  6:37893/149‑1 (MQ=255)                                                                                                                               ctcctGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGaaaaaa                                                                     >  6:207194/1‑99 (MQ=255)                                                                                                                               ctcctGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGaaaaaa                                                                     >  6:212192/1‑99 (MQ=255)                                                                                                                               ctcctGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAGGGGGTACgg                   >  3:368525/1‑149 (MQ=255)                                                                                                                                tcctGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAGGGGGTACGGc                  >  3:29063/1‑149 (MQ=255)                                                                                                                                     aGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAGGGGGTACGGCTTg               <  4:29063/147‑1 (MQ=255)                                                                                                                                        atGTTCACATTCGATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTg                                                                  >  4:173786/2‑93 (MQ=255)                                                                                                                                        atGTTCACATTCGATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTg                                                                  <  3:173786/92‑1 (MQ=255)                                                                                                                                         tGTTCACATTCGATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAGGGGGTACGGCTTg               <  1:64586/143‑1 (MQ=255)                                                                                                                                         tGTTCACATTCGATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAGGGGGTACGGCTTg               >  2:64586/1‑143 (MQ=255)                                                                                                                                         tGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAGGGGGTACGGCTTGGGTGAg         <  2:169137/149‑1 (MQ=255)                                                                                                                                              acaTCGAATGTGAACATTGATGTTCGTTTTCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAGGGGGTACGGCTTGGGTGAGGGGgc    <  5:73592/149‑1 (MQ=255)                                                                                                                                              acaTCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGa                                                         >  1:747/1‑96 (MQ=255)                                                                                                                                              acaTCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGa                                                         <  2:747/96‑1 (MQ=255)                                                                                                                                                aTCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAGGGGGTACGGCTTGGGTGAGGGGgcgc  >  3:311498/1‑149 (MQ=255)                                                                                                                                                aTCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAGGGGGTACGGCTTGGGTGAGGGGgcgc  >  3:190974/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                 CTTCAAGCCGCGACCAGCGGCGTCGGCAGCGATGCCCACGCCATTGATGCCACCGCCGATTACGGCAAGGTCATAGCAGTTGGCTGTTGGGGGCTGGGACGAAACTGGCTGGGACACGGGCAAGGCCTCCTGAGGCTGTTCACATCGAATGTGAACATTGATGTTCGTTTGCGAAAATATTAGCGCAACAGAACGCCGGTCGCCAGTCAGTCTCTATAGAAAAAACTGATCAAATGACAATGAAGTGAACATTTACGAAAATTCAGGGGGTACGGCTTGGGTGAGGGGGCGC  >  NC_002947/1232590‑1232881

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑