Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 152,157 A→T 8.7% intergenic (‑51/+43) PP_0143 ← / ← PP_0144 membrane protein/insulinase family metalloprotease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947152,1570AT 8.7% 121.3 / 3.5 46intergenic (‑51/+43)PP_0143/PP_0144membrane protein/insulinase family metalloprotease
Reads supporting (aligned to +/- strand):  ref base A (20/21);  new base T (2/2);  total (23/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.81e-01

CGGAGAGGAAACCGCCGAGCGCAATGATGCCCGGGTAGCGCTTGAGTAGTTCGATGATGCGCTGGACGATATGGGGGATTTGGGGCA‑TTGGGGGCTACGACAGAAAAGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGCACCACTGGGCCGTTCAGGTCCATGCCGCCGCCAGCGCAACAGCCCCGCCGCCAGGCCCAGCCCCACGAGCAAGGCCATCAACCCGTAG  >  NC_002947/152019‑152305
                                                                                                                                           |                                                                                                                                                    
cGGAGAGGAAACCGCCGAGCGCAATGATGCCCGGGTAGCGCTTGAGTAGTTCGATGATGCGCTGGACGATATGGGGGATTTGGGGCA‑TTGGGGGCTACGACAGAAAAGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGa                                                                                                                                             >  5:223854/1‑148 (MQ=255)
 ggAGAGGAAACCGCCGAGCGCAATGATGCCCGGGTAGCGCTTGAGTAGTTCGATGATGCGCTGGACGATATGGGGGATTTGGGGCA‑TTGGGGGCTACGACAGAAAAGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGa                                                                                                                                             >  2:265478/1‑147 (MQ=255)
       gAAACCGCCGAGCGCAATGATGCCCGGGTAGCGCTTGAGTAGTTCGATGATGCGCTGGACGATATGGGGGATTTGGGGCA‑TTGGGGGCTACGACAGAAAAGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCg                                                                                                                                     >  7:295777/1‑149 (MQ=255)
       gAAACCGCCGAGCGCAATGATGCCCGGGTAGCGCGTGAGTACGTCGATGATGCGCTGGACGATATGGGGGAGTTGGGGCA‑TTGGGCCCCACGACAGAAAAGTGAGGGGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCg                                                                                                                                     <  5:89733/149‑1 (MQ=255)
             gccgAGCGCAATGCTGCCCGGGTCGCGCTTGAGTAGTTCGTTGATGCGCTGGACGACAGGGGCGCTTTGGGTCC‑TTGCGCGCTACGACAGAAAAGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTg                                                                                                                               <  4:122646/149‑1 (MQ=255)
                     cAATGATGCCCGGGTAGCGCTTGAGTAGTTCGATGATGCGCTGGACGATATGGGGGATTTGGGGCA‑TTGGGGGCTACGACAGAAAAGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAgg                                                                                                                       <  1:169828/149‑1 (MQ=255)
                      aaTGATGCCCGGGTAGCGCTTGAGTAGTTCGATGATGCGCTGGGCGATATGGGGGATTTGGGGCA‑TTGGGGGCTACGACAGAAAAGTGAGGTGGCTGTGCCAGCCTCTTCGCCGGCCACCCCGCGAAAAGGTCGGTACTTGCAAAAgg                                                                                                                       >  6:64711/1‑148 (MQ=255)
                      aaTGATGCCCGGGTAGCGCTTGAGTAGTTCGATGATGCGCTGGACGATATGGGGGATTTGGGGCA‑TTGGGGGCTACGACAGAAAAGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGa                                                                                                                      >  3:79899/1‑149 (MQ=255)
                        tgatgCCCGGGTTGCGCTTGAGTTGTTCGATGTTGCGCTGGACGATATGGGGGATTTGGTGCA‑ATGGGGGCTACGACAGAAAAGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGa                                                                                                                      <  8:319302/147‑1 (MQ=39)
                             cccGGGTTGGGCTGGCGTAGTTCGATGTTGCGCTGGGCGATATGGGGGATTTGGGGCC‑TTGGGGGCTACGACAGAAAAGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAAGGTCGGTACTGGCAAAAGGATAccgc                                                                                                                <  5:64711/148‑1 (MQ=255)
                                            agtagtTCGATGATGCGCTGGACGATATGGGGGATTTGGGGCA‑TTGGGGGCTACGACAGAAAAGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGcc                                                                                                 <  1:265478/148‑1 (MQ=255)
                                             gtcgggcGAGGATGCGCTGGACGAGATGGGGGA‑TTGGGGCACTTGGGGGCTACGACAGAAAAGTGAGGGGGCTGTGCCAGGCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCgt                                                                                               <  8:295777/143‑1 (MQ=255)
                                                        atgCGCTGGACGATAGGGGGGATGTGGGGCA‑GTGGGGGCTACGACAGAAAAGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGcaccac                                                                                     <  6:223854/148‑1 (MQ=255)
                                                                gACGATATGGGGGATTTGGGGCA‑TTGGGGGCTACGACAGAAAAGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGCACCACTGGGCCGtt                                                                            >  7:288695/1‑149 (MQ=255)
                                                                     tatGGGGGATTTGGGGCA‑TTGGGGGCTACGACAGAAAAGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGCACCACTGGGCCGTTCAgg                                                                        <  4:271073/148‑1 (MQ=255)
                                                                     tatGGGGGATTTGGGGCA‑TTGGGGGCTACGACAGAAAAGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGCACCACTGGGCCGTTCAGGt                                                                       >  2:281286/1‑149 (MQ=255)
                                                                      atGGGGGATTTGGGGCA‑TTGGGGGCTACGACAGAAAAGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGCACCACTGGGCCGTTCAGGt                                                                       <  4:79899/148‑1 (MQ=255)
                                                                        gggggATTTGGGGCA‑TTGGGGGCTACGACAGATAAGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGCACCACTGGGCCGTTCAGGTCCa                                                                    >  5:93381/1‑149 (MQ=255)
                                                                          gggATTTGGGGCA‑TTGGGGGCTACGACAGAAAAGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGCACCACTGGGCCGTTCAGGTCCATg                                                                  >  7:149753/1‑149 (MQ=255)
                                                                              tttGGGGCA‑TTGGGGGCTACGACAGAAAAGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGCACCACTGGGCCGTTCAGGTCCATgccgc                                                              >  5:182600/1‑149 (MQ=255)
                                                                                                    aCAGAAAAGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGCACCACTGGGCCGTTCAGGTCCATGCCGCCGCCAGCGCAACAGCCccgcc                                         >  7:228076/1‑149 (MQ=255)
                                                                                                      aGAAAAGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGCACCACTGGGCCGTTCAGGTCCATGCCGCCGCCAGCGCAACAGCCCcgccgc                                       <  1:281286/149‑1 (MQ=255)
                                                                                                        aaaaGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGTCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGCACCACTGGGCCGTTCAGGTCCATGCCGCCGCCAg                                                        <  1:116071/130‑1 (MQ=255)
                                                                                                        aaaaGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGCACCACTGGGCCGTTCAGGTCCATGCCGCCGCCAg                                                        >  2:116071/1‑130 (MQ=255)
                                                                                                             tGAGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGCACCACTGGGcc                                                                               <  4:290246/102‑1 (MQ=255)
                                                                                                             tGAGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGCACCACTGGGcc                                                                               >  3:290246/1‑102 (MQ=255)
                                                                                                               aGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGCACCACTGGGCCGTTCAGGTCCATGCCGCCGCCAGCGCAACAGCCCCGCCGCCAGGCCCa                               <  8:149753/148‑1 (MQ=255)
                                                                                                                 gTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATGCCGCCGATTTACCGCTGCCGTGTAGCACCCCTGGGCCGTTCAGGTCCATGCCGCCGCCAGCGCAACAGCCCCGCCGCCAGGCCCAGcc                            >  5:295683/1‑149 (MQ=255)
                                                                                                                  tGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGCACCACTGGGCCGTTCAGGTCCATGCCGCCGCCAGCGCAACAGCCCCGCCGCCAGGCCCAGccc                           <  6:93381/149‑1 (MQ=255)
                                                                                                                      tgtgCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGCACCACTgg                                                                                  >  8:155686/1‑90 (MQ=255)
                                                                                                                      tgtgCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGCACCACTgg                                                                                  <  7:155686/90‑1 (MQ=255)
                                                                                                                       gtgCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGCACCACTGGGCCGTTCAGGTCCATGCCGCCGCCAGCGCAACAGCCCCGCCGCCAGGCCCAGCCCCAc                        >  3:222259/1‑147 (MQ=255)
                                                                                                                          ccAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGCACCACTGGGCCGTTCAGGTCCATGCCGCCGCCAGCGCAACAGCCCCGCCGCCAGGCCCAGCCCCACGAGCa                   <  4:222259/149‑1 (MQ=255)
                                                                                                                           cAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGCACCACTGGGCCGTTCAGGTCCATGCCGCCGCCAGCGCAACAGCCCCGCCGCCAGGCCCAGCCCCACGAGc                    >  4:75603/1‑147 (MQ=255)
                                                                                                                             gCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGCACCACTGGGCCGTTCAGGTCCATGCCGCCGCCAGCGCAACAGCCCCGCCGCCAGGCCCAGCCCCACGAGCAAgg                >  6:173843/1‑149 (MQ=255)
                                                                                                                               cctttCGCGGGCTTGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGCACCACTGGGCCGTTCAGGTCCATGCCGCCGCCAGCGCAACAGCCCCGCCGCCAGGCCCAGCCCCACGa                      <  7:173757/138‑1 (MQ=255)
                                                                                                                               cctttCGCGGGCTTGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGCACCACTGGGCCGTTCAGGTCCATGCCGCCGCCAGCGCAACAGCCCCGCCGCCAGGCCCAGCCCCACGa                      >  8:173757/4‑141 (MQ=255)
                                                                                                                                 tttCGCGGGCTTGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGCACCACTGGGCCGTTCAGGTCCATGCCGCCGCCAGCGCAACAGCCCCGCCGCCAGGCCCAGCCCCACGAGCAAGGCCa             >  8:12466/2‑148 (MQ=255)
                                                                                                                                   tCGCGGGCTTGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGCACCACTGGGCCGTTCAGGTCCATGCCGCCGCCAGCGCAACAGCCCCGCCGCCAGGCCCAGCCCCACGAGCAAGGCCa             <  7:12466/146‑1 (MQ=255)
                                                                                                                                    cgcgGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGCACCACTGGGCCGTTCAGGTCCATGCCGCCGCCAGCGCAACAGCCCCGCCGCCAGGCCCAGCCCCACGAGCAAGGCCAt            <  1:195569/146‑1 (MQ=255)
                                                                                                                                     gcgGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGCACCACTGGGCCGTTCAGGTCCATGCCGCCGCCAGCGCAACAGCCCCGCCGCCAGGCCCAGCCCCACGAGc                    >  4:196484/1‑137 (MQ=255)
                                                                                                                                     gcgGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGCACCACTGGGCCGTTCAGGTCCATGCCGCCGCCAGCGCAACAGCCCCGCCGCCAGGCCCAGCCCCACGAGc                    <  3:196484/137‑1 (MQ=255)
                                                                                                                                     gcgGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGCACCACTGGGCCGTTCAGGTCCATGCCGCCGCCAGCGCAACAGCCCCGCCGCCAGGCCCAGCCCCACGAGCAAGGCCa             <  8:302985/144‑1 (MQ=255)
                                                                                                                                     gcgGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGCACCACTGGGCCGTTCAGGTCCATGCCGCCGCCAGCGCAACAGCCCCGCCGCCAGGCCCAGCCCCACGAGCAAGGCCa             >  7:302985/1‑144 (MQ=255)
                                                                                                                                       gggCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGCACCACTGGGCCGTTCAGGTCCATGCCGCCGCCAGCGCAACAGCCCCGCCGCCAGGCCCAGCCCCACGAGCAAGGCCATCAAccc      >  3:236336/1‑149 (MQ=255)
                                                                                                                                          cAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGCACCACTGGGCCGTTCAGGTCCATGCCGCCGCCAGCGCAACAGCCCCGCCGCCAGGCCCAGCCCCACGAGCAAGGCCATCAACCCGTa   <  6:182600/149‑1 (MQ=255)
                                                                                                                                           aaGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGCACCACTGGGCCGTTCAGGTCCATGCCGCCGCCAGCGCAACAGCCCCGCCGCCAGGCCCAGCCCCACGAGCAAGGCCATCAACCCg     >  8:283710/1‑146 (MQ=255)
                                                                                                                                           aaGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGCACCACTGGGCCGTTCAGGTCCATGCCGCCGCCAGCGCAACAGCCCCGCCGCCAGGCCCAGCCCCACGAGCAAGGCCATCAACCCGTag  <  3:75603/149‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                                    
CGGAGAGGAAACCGCCGAGCGCAATGATGCCCGGGTAGCGCTTGAGTAGTTCGATGATGCGCTGGACGATATGGGGGATTTGGGGCA‑TTGGGGGCTACGACAGAAAAGTGAGGTGGCTGTGCCAGCCTCTTCGCGGGCAAGCCCGCGAAAGGGTCGGTACTGGCAAAAGGATACCGCCGATTTACCGCTGCCGTGTAGCACCACTGGGCCGTTCAGGTCCATGCCGCCGCCAGCGCAACAGCCCCGCCGCCAGGCCCAGCCCCACGAGCAAGGCCATCAACCCGTAG  >  NC_002947/152019‑152305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑