Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,241,531	C→T	7.1%	intergenic (+23/‑69)	PP_1080 → / → grxD	molybdopterin‑binding oxidoreductase/monothiol glutaredoxin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,241,531	0	C	T	7.1%	244.2 / 6.4	84	intergenic (+23/‑69)	PP_1080/grxD	molybdopterin‑binding oxidoreductase/monothiol glutaredoxin
Reads supporting (aligned to +/- strand):  ref base C (38/40);  new base T (3/3);  total (41/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.80e-01

CCTGCAGATCGCCCTGGCGCAACCCGGCGTGAGTGCCAATGACCTGACCGATGAGCGCTTTCGGGACCAGGTTTCCGGAAACGCTGCGC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCTACATGGATATCATCGAAACGATCAAAGAGCAGATTGCCAACAACACCATTCTGCTTTACATGAAAGGCTCGCCGAATGCC  >  NC_002947/1241385‑1241677                                                                                                                                                     |                                                                                                                                                   ccTGCAGATCGCCCTGGCGCAACCCGGCGTGAGTGCCAATGACCTGACCGATGAGCGCTTTCGGGACCAGGTTTCCGGAAACGCTGCGC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑Ggcgc                                                                                                                                                  >  4:319951/1‑149 (MQ=255)   tGCAGATCGCCCTGGCGCAACCCGGCGTGAGTGCCAATGACCTGACCGATGAGCGCTTTCGGGACCAGGTTTCCGGAAACGCTGCGC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTc                                                                                                                                                <  7:343073/149‑1 (MQ=255)   tGCAGATCGCCCTGGCGCAACCCGGCGTGAGTGCCAATGACCTGACCGATGAGCGCTTTCGGGACCAGGTTTCCGGAAACGCTGCGC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTc                                                                                                                                                >  8:343073/1‑149 (MQ=255)      aGATCGCCCTGGCGCAACCCGGCGTGAGTGCCAATGACCTGACCGATGAGCGCTTTCGGGACCAGGTTTCCGGAAACGCTGCGC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCGC‑CGTGCTCGGc                                                                                                                                             <  3:341621/149‑1 (MQ=255)        aTCGCCCTGGCGCAACCCGGCGTGAGTGCCAATGACCTGACCGATGAGCGCTTTCGGGACCAGGTTTCCGGAAACGCTGCGC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCtt                                                                                                                                           >  1:204140/1‑149 (MQ=255)           gCCCTTGCGCAACCCGGCGTGAGTGCCAATGACCTGACCGATGAGCGCTTTCGGGACCAGGTTTCCGGAAACGCTGCGC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTc                                                                                                                                         <  7:305909/148‑1 (MQ=255)           gCCCTGGCGCAACCCGGCGTGAGTGCCAATGACCTGACCGATGAGCGCTTTCGGGACCAGGTTTCCGGAAACGCTGCGC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCGC‑CGTGCTCGGcct                                                                                                                                           >  8:69982/1‑144 (MQ=255)           gCCCTGGCGCAACCCGGCGTGAGTGCCAATGACCTGACCGATGAGCGCTTTCGGGACCAGGTTTCCGGAAACGCTGCGC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCGC‑CGTGCTCGGcct                                                                                                                                           <  7:69982/146‑3 (MQ=255)           gCCCTGGCGCAACCCGGCGTGAGTGCCAATGACCTGACCGATGAGCGCTTTCGGGACCAGGTTTCCGGAAACGCTGCGC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTc                                                                                                                                         <  2:204140/148‑1 (MQ=255)            cccTGGCGCAACCCGGCGTGAGTGCCAATGACCTGACCGATGAGCGCTTTCGGGACCAGGTTTCCGGAAACGCTGCGC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTcc                                                                                                                                        >  6:161651/1‑148 (MQ=255)             ccTGGCGCAACCCGGCGTGAGTGCCAATGACCTGACCGATGAGCGCTTTCGGGACCAGGTTTCCGGAAACGCTGCGC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGa                                                                                                                                      >  1:199682/1‑149 (MQ=255)              cTGGCGCAACCCGGCGTGAGTGCCAATGACCTGACCGATGAGCGCTTTCGGGACCAGGTTTCCGGAAACGCTGCGC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGAt                                                                                                                                     <  5:84384/149‑1 (MQ=255)                 gcgcAACCCGGCGTGAGTGCCAATGACCTGACCGATGAGCGCTTTCGGGACCAGGTTTCCGGAAACGCTGCGC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATa                                                                                                                                    <  2:199682/147‑1 (MQ=255)                 gcgcAACCCGGCGTGAGTGCCAATGACCTGACCGATGAGCGCTTTCGGGACCAGGTTTCCGGAAACGCTGCGC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATAc                                                                                                                                   >  2:59870/1‑148 (MQ=255)                     aaCCCGGCGTGAGTGCCAATGACCTGACCGATGAGCGCTTTCGGGACCAGGTTTCCGGAAACGCTGCGC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGc                                                                                                                              >  8:135768/1‑149 (MQ=255)                                    ccAATGACCTGACCGATGAGCGCTTTCGGGACCAGGTTTCCGGAAACGCTGCGC‑TCAATGGCGTACCCTGGCAGGTGGAGGCGGATTGAGTAACGGAAAGGCCTCGCAC‑GGCGAACGGCTTGCCGATCAAATCCTAAACCGTCCcgacg                                                                                                               >  5:241558/1‑149 (MQ=255)                                    ccAATGACCTGACCGATGAGCGCTTTCGGGACCAGGTTTCCGGAAACGCTGCGC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCcgac                                                                                                                <  1:122391/148‑1 (MQ=255)                                         gacctgaccGATGAGCGCTTTCGGGACCAGGTTTCCGGAAACGCTGCGC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACtgt                                                                                                          >  5:369843/1‑149 (MQ=255)                                           cctgaccGATGAGCGCTTTCGGGACCAGGTTTCCGGAAACGCTGCGC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTgtgt                                                                                                        <  3:48683/149‑1 (MQ=255)                                            ctgaccGATGAGCGCTTTCGGGACCAGGTTTCCGGAAACGCTGCGC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTc                                                                                                       <  2:240038/149‑1 (MQ=255)                                            ctgaccGATGAGCGCTTTCGGGACCAGGTTTCCGGAAACGCTGCGC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTc                                                                                                       >  1:88436/1‑149 (MQ=255)                                             tgaccGATGAGCGCTTTCGGGACCAGGTTTCCGGAAACGCTGCGCGACAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCTAGCAC‑GCCGCTCGGCTTTCCGATACAATGAGACACCGTGCCGACGACTGAGTc                                                                                                       >  1:76950/1‑149 (MQ=255)                                             tgaccGATGAGCGCTTTCGGGACCAGGTTTCCGGAAACGCTGCGC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCg                                                                                                      >  8:334982/1‑149 (MQ=255)                                              gacaGATGAGCGCTTTCGGGACCAGGTTTCCGGAAACGCTGCGC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCg                                                                                                      >  6:275217/1‑148 (MQ=255)                                                cgtatcaGCGCTTTGGGTACCAGTTTTCCGTAACCGCTGATC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGa                                                                                                    <  1:123492/142‑1 (MQ=255)                                                   aTGAGCGCTTTCGGGACCAGGTTTCCGGAAACGCTGCGC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGt                                                                                                <  7:135768/149‑1 (MQ=255)                                                      aGCGCTTTCGGGACCAGGTTTCCGGAAACGCTGCGC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCa                                                                                             <  3:319951/149‑1 (MQ=255)                                                       gcgcTTTCGGGACCAGGTTTCCGGAAACGCTGCGC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTcc                                                                                                                                        >  3:162291/1‑105 (MQ=255)                                                       gcgcTTTCGGGACCAGGTTTCCGGAAACGCTGCGC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTcc                                                                                                                                        <  4:162291/105‑1 (MQ=255)                                                       gcgcTTTCGGGACCAGGTTTCCGGAAACGCTGCGC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAg                                                                                            >  3:43430/1‑149 (MQ=255)                                                       gcgcTTTCGGGACCAGGTTTCCGGAAACGCTGCGC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAg                                                                                            >  6:301727/1‑149 (MQ=255)                                                            ttCGGGACCAGGTTTCCGGAAACGCTGCGC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAg                                                                                       >  3:240925/1‑149 (MQ=255)                                                             tCGGGACCAGGTTTCCGGAAACGCTGCGC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAg                                                                                       <  6:369843/148‑1 (MQ=255)                                                               gggACCAGGTTTCCGGAAACGCTGCGC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAg                                                                                                 >  8:329044/1‑136 (MQ=255)                                                               gggACCAGGTTTCCGGAAACGCTGCGC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAg                                                                                                 <  7:329044/136‑1 (MQ=255)                                                               gggACCAGGTTTCCGGAAACGCTGCGC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGt                                                                                     <  5:161651/148‑1 (MQ=255)                                                                    cAGGTTTCCGGAAACGCTGCGC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCt                                                                                  <  5:77131/146‑1 (MQ=255)                                                                    cAGGTTTCCGGAAACGCTGCGC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCt                                                                                  >  6:77131/1‑146 (MQ=255)                                                                    cAGGTTTCCGGAAACGCTGCGC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCTACa                                                                               >  5:113916/1‑149 (MQ=255)                                                                    cAGGTTTCCGGAAACGCTGCGC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCTACa                                                                               >  1:8348/1‑149 (MQ=255)                                                                     ctggttCCGGAAACGCGGCGC‑CCAATTGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCTACAt                                                                              <  6:241558/145‑1 (MQ=255)                                                                     aGGTTTCCGGAAACGCTGCGC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCTACAt                                                                              <  7:334982/149‑1 (MQ=255)                                                                       gTTTCCGGAAACGCTGCGC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCTACATg                                                                             >  4:28528/1‑148 (MQ=255)                                                                            cGGAAACGCTGCGC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCTACATgg                                                                            <  1:322516/144‑1 (MQ=255)                                                                            cGGAAACGCTGCGC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCTACATgg                                                                            >  2:322516/1‑144 (MQ=255)                                                                             ggAATCGCTGCGC‑TGAATGGCGTACCCGTGGAGGTCGAGGCGGCTTGAGGTACGGCAAGGCCGAGGAC‑GTCTATCGTCTTTCCGATAGAATGGGACACGGTGCGGACGACTGTGTCGGATAGTTCAGCCGATGTGCTACATGGATatca                                                                      <  2:76950/149‑1 (MQ=255)                                                                              gAAACGCTGCGC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCTACATGGat                                                                          <  6:334362/144‑1 (MQ=255)                                                                              gAAACGCTGCGC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCTACATGGat                                                                          >  5:334362/1‑144 (MQ=255)                                                                              gAAACGCTGCGC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCTACATGGATatcat                                                                     <  6:15160/149‑1 (MQ=255)                                                                                             aaTGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCTACATGGATATCATCGAAACGATCa                                                          >  2:196464/1‑146 (MQ=255)                                                                                               tGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCTACATGGATATCATCGAAACGATCAAagag                                                     <  3:123427/149‑1 (MQ=255)                                                                                                 gCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGGGCTACATGGATATCATCGAAACGATCAAAGAGCa                                                   <  5:301727/149‑1 (MQ=255)                                                                                                     aCCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCTACATGGATATCATCAAAACGATCAAAGAGCAGAtt                                               >  4:241593/1‑149 (MQ=255)                                                                                                      cccGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCTACATGGATATCATCGAAACGATCAAAGAGCAGAtt                                               <  5:268003/148‑1 (MQ=255)                                                                                                      cccGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCTACATGGATATCATCGAAACGATCAAAGAGCAGAtt                                               <  3:28528/148‑1 (MQ=255)                                                                                                                tCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCTACATGGATATCATCGAAACGATCAAAGAGCAGATTGCCAACAacac                                    >  1:336992/1‑149 (MQ=255)                                                                                                                tCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCTACATGGATATCATCGAAACGATCAAAGAGCAGATTGCCAACAacac                                    <  5:275217/149‑1 (MQ=255)                                                                                                                 cGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCTACATGGATATCATCGAAACGATCAAAGAGCAGATTGCCAACAacac                                    >  5:33637/1‑148 (MQ=255)                                                                                                                 cGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCTACATGGATATCATCGAAACGATCAAAGAGCAGATTGCCAACAacac                                    >  8:143540/1‑148 (MQ=255)                                                                                                                  gAGGCCGCTTGAGGAACGGCAAGGCCGAGCACTGGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCTACATGGATATCATCGAAACGATCAAAGAGCAGATTGCCAACAacac                                    >  1:232255/1‑148 (MQ=255)                                                                                                                     gCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCTATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCTACATGGATATCATCGAAACGATCAAAGAGCAGATTGCCAACAACACCATTc                               <  3:34636/149‑1 (MQ=255)                                                                                                                       cGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCTACATGGATATCATCGAAACGATCAAAGAGCAGATTGCCAACAACACCATTCTg                             >  3:158598/1‑149 (MQ=255)                                                                                                                         cTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCTACATGGATATCATCGAAACGATCAAAGAGCAGATTGCCAACAACACCATTCTGCt                           <  4:43430/149‑1 (MQ=255)                                                                                                                              ggAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCTACATGGATATCATCGAAACGATCAAAGAGCAGATTGCCAACAACACCATTCTGCTTTACa                      <  3:220742/149‑1 (MQ=255)                                                                                                                              ggAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCTACATGGATATCATCGAAACGATCAAAGAGCAGATTGCCAACAACACCATTCTGCTTTACa                      >  4:220742/1‑149 (MQ=255)                                                                                                                              ggAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACGGTGTCGGATAGTTCAGCCGAGGTGCTACATGGATATCATCGAAACGATCAAAGAGCAGATTGCCAACAACACCATTCTGCTTTACa                      <  2:88436/149‑1 (MQ=255)                                                                                                                               gAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCTACATGGATATCATCGAAACGATCAAAGAGCAGATTGCCAacaaca                                     <  2:187894/133‑1 (MQ=255)                                                                                                                               gAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCTACATGGATATCATCGAAACGATCAAAGAGCAGATTGCCAacaaca                                     >  1:187894/1‑133 (MQ=255)                                                                                                                               gAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCTACATGGATATCATCGAAACGATCAAAGAGCAGATTGCCAACAACACCATTCTGCTTTACAt                     >  6:293763/1‑149 (MQ=255)                                                                                                                                   ggCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCTACATGGATATCATCGAAACGATCAAAGAGCAGATTGCCAACAACACCATTCTGCTTTACATGaaa                 <  6:113916/149‑1 (MQ=255)                                                                                                                                   ggCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCTACATGGATATCATCGAAACGATCAAAGAGCAGATTGCCAACAACACCATTCTGCTTTACATGaaa                 <  2:336992/149‑1 (MQ=255)                                                                                                                                   ggCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCTACATGGATATCATCGAAACGATCAAAGAGCAGATTGCCAACAACACCATTCTGCTTTACATGaaa                 <  5:293763/149‑1 (MQ=255)                                                                                                                                    ggaaagCCGAGCGC‑CGTGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCTACATGGATATCATCGAAACGATCAAAGAGCAGATTGCCAACAACACCATTCTGCTTTACATGAAAg                >  3:300700/6‑149 (MQ=255)                                                                                                                                    gCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCTACATGGATATCATCGAAACGATCAAAGAGCAGATTGCCAACAACACCATTCTGCTTTACATGAAAg                >  6:241269/1‑149 (MQ=255)                                                                                                                                    gCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCTACATGGATATCATCGAAACGATCAAAGAGCAGATTGCCAACAACACCATTCTGCTTTACATGAAAg                >  6:311279/1‑149 (MQ=255)                                                                                                                                     gaaagCCGAGCGC‑CGTGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCTACATGGATatcat                                                                     <  7:279449/91‑1 (MQ=255)                                                                                                                                     gaaagCCGAGCGC‑CGTGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCTACATGGATatcat                                                                     >  8:279449/5‑95 (MQ=255)                                                                                                                                        ggCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCTACATGGat                                                                          <  6:155969/87‑1 (MQ=255)                                                                                                                                        ggCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCTACATGGat                                                                          >  5:155969/1‑87 (MQ=255)                                                                                                                                          ccGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCTACATGGATATCATCGAAACGATCAAAGAGCAGATTGCCAACAACACCATTCTGCTTTACATGAAAGGCTCGc          <  5:311279/149‑1 (MQ=255)                                                                                                                                          ccGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCTACATGGATATCATCGAAACGATCAAAGAGCAGATTGCCAACAACACCATTCTGCTTTACATGAAAGGCTCGc          <  4:240925/149‑1 (MQ=255)                                                                                                                                          ccGAGCAC‑GGCGCTCGGCTTTCCGATAAAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCTACATGGATATCATCGAAACGATCAAAGAGCAGATTGCCAACAACACCATTCTGCTTTACATGAAAGGCTCGc          <  2:295819/149‑1 (MQ=255)                                                                                                                                            gAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCTACATGGATATCATCGAAACGATCAAAGAGCAGATTGCCAACAACACCATTCTGCTTTACATGAAAGGCTCGcc         >  7:111901/1‑148 (MQ=255)                                                                                                                                               cAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCTACATGGATATCATCGAAACGATCAAAGAGCAGATTGCCAACAACACCATTCTGCTTTACATGAAAGGCTCGGCGAAt     <  1:59870/149‑1 (MQ=255)                                                                                                                                                   ggCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCTACATGGATATCATCGAAACGATCAAAGAGCAGATTGCCAACAACACCATTCTGCTTTACATGAAAGGCTCGCCGAATGcc  >  2:262281/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                   CCTGCAGATCGCCCTGGCGCAACCCGGCGTGAGTGCCAATGACCTGACCGATGAGCGCTTTCGGGACCAGGTTTCCGGAAACGCTGCGC‑TCAATGGCGTACCCGTGCAGGTCGAGGCCGCTTGAGGAACGGCAAGGCCGAGCAC‑GGCGCTCGGCTTTCCGATACAATGCGACACCGTGCCGACGACTGTGTCGGATAGTTCAGCCGAGGTGCTACATGGATATCATCGAAACGATCAAAGAGCAGATTGCCAACAACACCATTCTGCTTTACATGAAAGGCTCGCCGAATGCC  >  NC_002947/1241385‑1241677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑