Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,244,369 C→G 14.1% G39A (GGT→GCT) ‡ rnt ← ribonuclease T

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,244,3690CG14.1% 169.7 / 18.8 64G39A (GGT→GCT) ‡rntribonuclease T
Reads supporting (aligned to +/- strand):  ref base C (28/27);  new base G (6/3);  total (34/30)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.22e-01

TCAAGTGCGGCCGGCTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCAGCGCAGT  >  NC_002947/1244222‑1244514
                                                                                                                                                   |                                                                                                                                                 
tCAAGTGCGGCCGGCTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAaccacc                                                                                                                                                  >  2:203812/1‑149 (MQ=255)
  aaGTGCGGCCGGCTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCgg                                                                                                                                                >  5:146353/1‑149 (MQ=255)
          ccggcTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCGGTGGTTTCAACa                                                                                                                                        <  1:66952/149‑1 (MQ=255)
          ccggcTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACa                                                                                                                                        <  6:146353/149‑1 (MQ=255)
             gcTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATcc                                                                                                                                     <  5:218812/149‑1 (MQ=255)
               tCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATccac                                                                                                                                   >  6:367101/1‑149 (MQ=255)
                    gTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGa                                                                                                                              >  7:101824/1‑149 (MQ=255)
                             cTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGa                                                                                                                      >  7:269892/1‑148 (MQ=255)
                                 aaCGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGcc                                                                                                                 >  2:84423/1‑149 (MQ=255)
                                   cGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTACAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCcgc                                                                                                               <  8:269892/149‑1 (MQ=255)
                                         cACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGaa                                                                                                           <  1:203812/147‑1 (MQ=255)
                                         cACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGaa                                                                                                           <  6:235029/147‑1 (MQ=255)
                                         cACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGaa                                                                                                           <  4:177397/147‑1 (MQ=255)
                                          aCTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAgcg                                                                                                        >  7:14947/1‑149 (MQ=255)
                                          aCTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAgcg                                                                                                        >  3:162928/1‑149 (MQ=255)
                                                    aGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATc                                                                                              <  8:101824/149‑1 (MQ=255)
                                                     gTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGCCCGGCAGATAGCCGCGGAAGCGCTCGGCCATCg                                                                                             >  8:172603/1‑149 (MQ=255)
                                                         gTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTg                                                                                         >  7:269211/1‑149 (MQ=255)
                                                             gTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATc                                                                                              <  1:82881/140‑1 (MQ=255)
                                                             gTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGAAATGCATCGGTAGAGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATc                                                                                              >  2:82881/1‑140 (MQ=255)
                                                               tCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAg                                                                                    >  8:148722/1‑148 (MQ=255)
                                                                 ggggAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGAc                                                                                 <  3:151191/149‑1 (MQ=255)
                                                                  gggAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGAcc                                                                                >  5:197718/1‑149 (MQ=255)
                                                                          gAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCtgctgc                                                                        >  5:130307/1‑149 (MQ=255)
                                                                               cTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTgg                                                                   <  7:148722/149‑1 (MQ=255)
                                                                               cTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTgg                                                                   <  5:367101/149‑1 (MQ=255)
                                                                                tttCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAgg                                                                                   <  8:156738/132‑1 (MQ=255)
                                                                                tttCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTggg                                                                  >  7:19568/1‑149 (MQ=255)
                                                                                  tctcGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGgt                                                                 <  6:197718/148‑1 (MQ=255)
                                                                                  tctcGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGgt                                                                 <  1:84423/148‑1 (MQ=255)
                                                                                      gTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCTGGTGGCGAGGACCGCTGCTGCCTTGGGTGTc                                                              >  3:332895/1‑147 (MQ=255)
                                                                                      gTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTcc                                                             <  8:269211/148‑1 (MQ=255)
                                                                                        ccATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAgg                                                          >  7:117958/1‑149 (MQ=255)
                                                                                         cATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGgt                                                         >  3:367030/1‑149 (MQ=255)
                                                                                          aTGCCAATGGTGACGGCGGCAATTTCCAGGAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGgtc                                                        <  8:215718/149‑1 (MQ=255)
                                                                                          aTGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCGGgtc                                                        >  6:75198/1‑149 (MQ=255)
                                                                                          aTGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGgtc                                                        <  8:14947/149‑1 (MQ=255)
                                                                                          aTGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGgtc                                                        <  4:162928/149‑1 (MQ=255)
                                                                                               aaTGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCGGGTCGTCtt                                                   <  5:75198/149‑1 (MQ=255)
                                                                                                             cAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCt                                                    >  5:341694/1‑134 (MQ=255)
                                                                                                             cAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCt                                                    <  6:341694/134‑1 (MQ=255)
                                                                                                                tttCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCg                                                                               >  6:53272/1‑104 (MQ=255)
                                                                                                                tttCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCg                                                                               <  5:53272/104‑1 (MQ=255)
                                                                                                                 ttCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCa                                 >  4:169216/1‑149 (MQ=255)
                                                                                                                   ccAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCCTAAAGGTCTTCGCACAcg                               >  4:86629/1‑149 (MQ=255)
                                                                                                                         aTGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGtttt                         <  4:332895/149‑1 (MQ=255)
                                                                                                                            cATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTtct                      >  1:252490/1‑149 (MQ=255)
                                                                                                                             aTCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAgg                                                          >  5:373983/1‑112 (MQ=255)
                                                                                                                             aTCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAgg                                                          <  6:373983/112‑1 (MQ=255)
                                                                                                                                ggTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCca                   <  2:252490/148‑1 (MQ=255)
                                                                                                                                 gTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCcag                  >  2:190749/1‑148 (MQ=255)
                                                                                                                                    gcgcTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTgg                                                                   <  1:155453/96‑1 (MQ=255)
                                                                                                                                    gcgcTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTgg                                                                   >  2:155453/1‑96 (MQ=255)
                                                                                                                                    gcgcTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAgg              <  1:25409/149‑1 (MQ=255)
                                                                                                                                     ggatGTTGAAACCGGTGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGgc             >  8:89559/4‑149 (MQ=255)
                                                                                                                                        tGTTGAAACCGGTGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTtct                      <  6:286026/137‑1 (MQ=255)
                                                                                                                                        tGTTGAAACCGGTGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTtct                      >  5:286026/1‑137 (MQ=255)
                                                                                                                                         gTTGAAACCGGTGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGgcgc           >  8:106569/1‑147 (MQ=255)
                                                                                                                                          ttGAAACCGGTGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCAg        >  5:373782/1‑149 (MQ=255)
                                                                                                                                          ttGAAACCGGTGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCAg        >  4:317750/1‑149 (MQ=255)
                                                                                                                                           tGAAACCGGTGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGgt                                                         <  6:325059/99‑1 (MQ=255)
                                                                                                                                           tGAAACCGGTGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGgt                                                         >  5:325059/1‑99 (MQ=255)
                                                                                                                                            gAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGtt                           <  6:277560/128‑1 (MQ=255)
                                                                                                                                            gAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGtt                           >  5:277560/1‑128 (MQ=255)
                                                                                                                                            gAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCAgcg      >  5:3650/1‑149 (MQ=255)
                                                                                                                                                ccaccGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCAGCGCAGt  >  4:293800/1‑149 (MQ=255)
                                                                                                                                                  accGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCca                   >  2:274652/1‑130 (MQ=255)
                                                                                                                                                  accGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCca                   <  1:274652/130‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TCAAGTGCGGCCGGCTCGATGTTGGCGCCCTCGAACGGCTCCACTCGGTGGAAGTAGGTATGTTCGGGGAACAGGAAACCTTTCTCGTCCATGCCAATGGTGACGGCGGCAATTTCCAGCAATGCATCGGTAGCGCTGTTGAAACCACCGGTTTCAACATCCACCACGACCGGCAGATAGCCGCGGAAGCGCTCGGCCATCGGGTGGCGAGGACCGCTGCTGCCTTGGGTGTCCAGGTCGTCTTCGTAAAGGTCTTCGCTCACGCGTTTTTCTCCAGCAGGCGCCAGCGCAGT  >  NC_002947/1244222‑1244514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑