Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,273,533 A→C 7.7% H14Q (CAT→CAG)  PP_1114 ← SEC‑C domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,273,5330AC 7.7% 234.8 / 6.6 75H14Q (CAT→CAG) PP_1114SEC‑C domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (27/42);  new base C (3/3);  total (30/45)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGATTATCTCACCACTGCGCGAGATGTGCAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCAGGGA  >  NC_002947/1273387‑1273680
                                                                                                                                                   |                                                                                                                                                    
tGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATg                                                                                                                                                    >  1:20532/1‑149 (MQ=255)
  gCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGTGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATgg                                                                                                                                                   <  2:200112/148‑1 (MQ=255)
  gCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATgg                                                                                                                                                   <  6:179110/148‑1 (MQ=255)
    aCTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTTCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTg                                                                                                                                                <  5:118414/149‑1 (MQ=255)
    aCTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTg                                                                                                                                                >  4:302670/1‑149 (MQ=255)
         ttGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGt                                                                                                                                           >  2:90390/1‑148 (MQ=255)
            tACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAg                                                                                                                                        <  1:364116/148‑1 (MQ=255)
            tACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAg                                                                                                                                        <  4:78416/148‑1 (MQ=255)
            tACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAg                                                                                                                                        >  2:364116/1‑148 (MQ=255)
             aCTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAggg                                                                                                                                      >  6:40538/1‑149 (MQ=255)
             aCTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAggg                                                                                                                                      >  5:377205/1‑149 (MQ=255)
              cttcttCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAGGGc                                                                                                                                     <  5:189699/149‑1 (MQ=255)
              cttcttCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGGGGGGGCTGATCATGGCCATGGTTGC‑AGTCAgg                                                                                                                                       <  1:191918/148‑1 (MQ=255)
               ttcttcTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAGGGc                                                                                                                                     >  2:272975/1‑148 (MQ=255)
                 gttgtcGCTACCGCTCGGGCAGGGGTCGTTACGGACAACGTCCTTCAGGGCGTTGAGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGACATGGTTGC‑AGTCAGGGc                                                                                                                                     <  3:58676/142‑1 (MQ=255)
                  ttctCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAGGGCCAt                                                                                                                                  >  2:234579/1‑148 (MQ=255)
                    ctcGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAGGGCCAt                                                                                                                                  <  8:297809/146‑1 (MQ=255)
                       gCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCCTGACTGC‑AACCATGGCCATGatc                                                                                                                              <  5:380750/147‑3 (MQ=255)
                       gCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTg                                                                                                                                                <  7:201389/130‑1 (MQ=255)
                       gCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTg                                                                                                                                                >  8:201389/1‑130 (MQ=255)
                       gCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAGGGCCATGAAc                                                                                                                              <  4:156713/147‑1 (MQ=255)
                        cTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAGGGCCATGAACGTg                                                                                                                           <  3:28814/149‑1 (MQ=255)
                           ccGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAGGGCCATGAACGTgggg                                                                                                                        <  7:220083/149‑1 (MQ=255)
                           ccGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAGGGCCATGAACGTggg                                                                                                                         <  7:5205/148‑1 (MQ=255)
                             gcacgggcacggGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAGGGcc                                                                                                                                    <  2:171596/135‑1 (MQ=255)
                             gcacgggcacggGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAGGGcc                                                                                                                                    >  1:171596/1‑135 (MQ=255)
                               acgggcacgggTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAGGGCCATGAACGTGGGgttgt                                                                                                                    <  6:377205/149‑1 (MQ=255)
                                  ggcacgggTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGTTCATGGCCATGGTTGC‑AGTCAGGGCCATGAACGTGGGGTTGTTgg                                                                                                                 <  6:321381/149‑1 (MQ=255)
                                  ggcacgggTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAGGGCCATGAACGTGGGGTTGTTgg                                                                                                                 <  6:128371/149‑1 (MQ=255)
                                       gggTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAGGGCCATGAACGTGGGGTTGTTGGGACAt                                                                                                            >  7:39097/1‑149 (MQ=255)
                                        ggTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAGGGCCATGAACGTGGGGTTGTTGGGACAtt                                                                                                           <  6:294905/149‑1 (MQ=255)
                                          tCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGc                                                                                                         <  6:333570/149‑1 (MQ=255)
                                            gTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCa                                                                                                        <  3:118168/148‑1 (MQ=255)
                                            gTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAg                                                                                                       <  1:90390/149‑1 (MQ=255)
                                               aCGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGGGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAAGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGt                                                                                                    >  6:253005/1‑149 (MQ=255)
                                                  gCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGACCCTGACTGC‑AACCATGGCCATGat                                                                                                                               >  8:64955/1‑118 (MQ=255)
                                                  gCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGACCCTGACTGC‑AACCATGGCCATGat                                                                                                                               <  7:64955/119‑2 (MQ=255)
                                                  gCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCAGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAGGGCCATGAACGTGGGGTTGTTGGGACGTTGCAGGGTCAc                                                                                                 >  7:130737/1‑149 (MQ=255)
                                                   ccAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACt                                                                                                <  1:234579/149‑1 (MQ=255)
                                                    cAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTc                                                                                               <  5:21658/149‑1 (MQ=255)
                                                     aaCGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAgg                                                                                                                                       <  5:147706/108‑1 (MQ=255)
                                                     aaCGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAgg                                                                                                                                       >  6:147706/1‑108 (MQ=255)
                                                     aaCGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTcc                                                                                              >  8:167602/1‑149 (MQ=255)
                                                        gaccTTGAGGTCGTTGCGTATCGGCGCCTGGTGGCCGTGGTTGCAGTGCGGGGCTTTTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAGGGCCATGAACGTGTGGTTGTTGGGACATTGCAGGGTCACTCCGGt                                                                                           <  7:333009/147‑1 (MQ=255)
                                                        gTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGt                                                                                           <  1:272975/149‑1 (MQ=255)
                                                                  gCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAAtcgt                                                                                 <  5:40538/149‑1 (MQ=255)
                                                                        cGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGa                                                                           <  2:20532/149‑1 (MQ=255)
                                                                         gTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTTGTGG‑TGATCATGGCCATGGTTGC‑AGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGAt                                                                          >  7:238103/1‑149 (MQ=255)
                                                                                    tggtggCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCgg                                                                              >  2:44879/1‑134 (MQ=255)
                                                                                    tggtggCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCgg                                                                              <  1:44879/134‑1 (MQ=255)
                                                                                      gtggCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAGGGCCATGAACGTGTGGTTGTTGGGACATTGCAGGGTCCCTACGGTATGTAAGCGTCGGGGATTATCTCACCACTg                                                             >  6:214459/1‑149 (MQ=255)
                                                                                         gCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGAtt                                                                         <  4:126866/134‑1 (MQ=255)
                                                                                         gCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGAtt                                                                         >  3:126866/1‑134 (MQ=255)
                                                                                             tGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTTGTGG‑TGATCATGGCCATGGTTGC‑AGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGATTATCTCACCACTGCGCgaga                                                      <  8:238103/149‑1 (MQ=255)
                                                                                               gTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCgg                                                                              >  2:284108/1‑123 (MQ=255)
                                                                                               gTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCgg                                                                              <  1:284108/123‑1 (MQ=255)
                                                                                                   cAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAgg                                                                                                      <  8:328296/95‑1 (MQ=255)
                                                                                                   cAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAgg                                                                                                      >  7:328296/1‑95 (MQ=255)
                                                                                                       gCGGGCCGTGTACATGGCCGGGGGCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGATTATCTCACCACTGCGCGAGATGTGCAGGTc                                            <  5:214459/149‑1 (MQ=255)
                                                                                                         gggCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGATTATCTCACCACTGCGCGAGATGTGCAGGTccc                                          <  3:302670/149‑1 (MQ=255)
                                                                                                                 tACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGATTATCTCACCACTGCGTGAGATGTGCAGGTCCCgatggat                                   <  7:167602/148‑1 (MQ=255)
                                                                                                                 tACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGATTATCTCACCACTGCGCGAGATGTGCAGGTCCCgatggatg                                  <  8:39097/149‑1 (MQ=255)
                                                                                                                    aTGGCCGGTGGCGTTGTGG‑TGATCATGGCCATGTTTGC‑AGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGGCACTCCGGTATGTAATCGTCGGGGATTATCTCACCACTGCGCGAGATGTGCAGGTCCCGATGGATGAg                                <  7:59526/148‑1 (MQ=255)
                                                                                                                           tggtcgtggtGG‑TGATCATGGCCATGGTTGC‑AGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGATTATCTCACCACTGCGCGAGATGTGCAGGTCCCGATGGATGAGCAACCCgg                        <  7:229683/149‑1 (MQ=255)
                                                                                                                               cgtggtGG‑TGATCATGGCCATGGTTGC‑AGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGATTATCTCACCACTGCGCGAGATGTGCAGGTCCCgatggatg                                  <  8:293176/135‑1 (MQ=255)
                                                                                                                               cgtggtGG‑TGATCATGGCCATGGTTGC‑AGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGATTATCTCACCACTGCGCGAGATGTGCAGGTCCCgatggatg                                  >  7:293176/1‑135 (MQ=255)
                                                                                                                                gtggtgg‑tgATCATGGCCATGGTTGCGAGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGATTATCTCACCACTGCGCGAGATGTGCAGGTCCCGATGGATGAGCAACCCGGTCGc                    >  1:5425/1‑149 (MQ=255)
                                                                                                                                      cacgttCATGGCCCTGACTGC‑AACCATGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATATAATCGTCGGGGATTATCTCACCACTGCGCGAGATGTGCAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTc              >  5:174912/6‑149 (MQ=38)
                                                                                                                                         gttCATGGCCCTGACTGC‑AACCATGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGATTATCTCACCACTGCGCGAGATGTGCAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGcc           <  5:81699/147‑1 (MQ=255)
                                                                                                                                         gttCATGGCCCTGACTGC‑AACCATGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGATTATCTCACCACTGCGCGAGATGTGCAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGcc           >  6:81699/3‑149 (MQ=255)
                                                                                                                                         gATCATGGCCATGGTTGC‑AGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGATTATCTCACCACTGCGCGAGATGTGCAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGcc           >  7:155664/1‑149 (MQ=255)
                                                                                                                                             aTGGCCATGGTTGC‑AGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGATTATCACACCACTGCGCGAGATGTGCAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGCCCtc        >  3:206924/1‑147 (MQ=255)
                                                                                                                                              tGGCCATGGTTGC‑AGTCAAGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGATTATCTCACCACTGCGCGAGGTGTGCAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCa      <  5:253005/149‑1 (MQ=255)
                                                                                                                                                gCCATGGTTGC‑AGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGATTATCTCACCACTGCGCGAGATGTGCAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCAgg    >  4:202949/1‑149 (MQ=255)
                                                                                                                                                gCCATGGTTGC‑AGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGATTATCTCACCACTGCGCGAGATGTGCAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCAgg    >  8:97456/1‑149 (MQ=255)
                                                                                                                                                   aTGGTTGC‑AGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGATTATCTCACCACTGCGCGAGATGTGCAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCAgg    >  6:256543/1‑146 (MQ=255)
                                                                                                                                                   aTGGTTGC‑AGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGATTATCTCACCACTGCGCGAGATGTGCAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCAGGGa  <  5:256543/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TGGCACTTCTTGTACTTCTTCTCGCTACCGCACGGGCACGGGTCGTTACGGCCAACGTCCTTCAGGGCGTTGCGTATCGGCTCCTGGTGGCCGTGGTTGCAGTGCGGGCCGTGTACATGGCCGTGGTCGTGGTGG‑TGATCATGGCCATGGTTGC‑AGTCAGGGCCATGAACGTGGGGTTGTTGGGACATTGCAGGGTCACTCCGGTATGTAATCGTCGGGGATTATCTCACCACTGCGCGAGATGTGCAGGTCCCGATGGATGAGCAACCCGGTCGCCAGTTCGCCCTGCAGGGA  >  NC_002947/1273387‑1273680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑