Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,275,084 G→C 11.4% intergenic (‑281/+31) PP_5462 ← / ← PP_5463 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,275,0840GC11.4% 112.1 / 8.1 44intergenic (‑281/+31)PP_5462/PP_5463hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (22/16);  new base C (3/2);  total (25/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.09e-01

CGAATGGGCATTTTGGTCTATCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGTTGTATCCAGCCTGT  >  NC_002947/1274942‑1275229
                                                                                                                                              |                                                                                                                                                 
cgtaTGTGCATTTTGGTCTATCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATgggc                                                                                                                                             <  6:202167/146‑1 (MQ=255)
    tGGGCATTTTGGTCTATCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATgggcgggc                                                                                                                                         <  1:16427/149‑1 (MQ=255)
         aTTTTGGTCTATCCGGGGGAGAGTGTATGTCGAAGCCTGCTTCCCGTTTACGTCGGCTCAAATAAGTTGCGGGAGAGCGGTAATGATTGGGGTGATGGTTGTAAATGTTAAAGGAGAATGAGCAAAGCCCGCCCATCGGCGGGCtttgc                                                                                                                                    <  8:141680/149‑4 (MQ=255)
         aTTTTGGTCTATCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCCATCGGCGGGCtttgc                                                                                                                                    <  3:222618/149‑4 (MQ=255)
                tCTATCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGGGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAgggggggg                                                                                                                             <  4:303387/149‑1 (MQ=255)
                    gccGGCGGACCCTGAATGGCGAAGCCTGCTTCCCGGTGACGTCGGCTCCAATCAGGCGCGTGAGCGCGGTACTGATGGGGGTGCTGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAgggggcgcca                                                                                                                           <  6:120622/146‑6 (MQ=255)
                       ggcggAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTACTGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAgg                                                                                                                      <  5:209989/149‑1 (MQ=255)
                        gctgagcGTGAATGGCGAAGGCTGCTCCCGGGGTACGGGCGCTCCAAGTTGGTCCGGGCTAGCCGTAATGATGGGGGTGATGGGGGTACCGGTGAAAGGAGAATGAGCAAACCCCGCCGAGGGGCGGGCTTCAGGGGGGGGCAGAAggg                                                                                                                     <  2:57518/146‑1 (MQ=255)
                          ggAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAggg                                                                                                                                  >  8:335733/1‑134 (MQ=255)
                          ggAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAggg                                                                                                                                  <  7:335733/134‑1 (MQ=255)
                                   aTGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAATCACGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGtacta                                                                                                          >  7:295076/1‑149 (MQ=255)
                                   aTGTCGAAGCCTGCTTCCCGGTTACGTCGGCTACAATAAGGTGCGGGACCTCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAAAGACCAAACACCGCCGATGGGCGGGCTTCTGGGGGGGGCAGCAGGGCTATCGtacta                                                                                                          >  5:58595/1‑149 (MQ=255)
                                             gtGCTTCCCGGTTACTTCTGCTACATTAATTTGCGGGAGAGGGGTAATGATGTGTGTGAGGTGGGTATCGCTGAAATGTCTAAGAGCTAAGCCCGCCTTTGGGCGGGCTTCAGGTGGGGGCAGAAGGGCTATCGTACTACGGTCGCTtc                                                                                                <  4:90832/148‑1 (MQ=255)
                                             cTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGGGGGCTTCAGGGGGGGGCAGAAGGGGGATCGTACTACGGTCGGTtc                                                                                                >  3:90832/1‑149 (MQ=255)
                                                                     aaTAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAgg                                                                                 >  7:162695/1‑140 (MQ=255)
                                                                     aaTAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCa                                                                        >  2:166161/1‑149 (MQ=255)
                                                                      aTAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCAc                                                                       >  5:190703/1‑149 (MQ=255)
                                                                          ggTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTa                                                                               >  1:299827/1‑137 (MQ=255)
                                                                          ggTGCGGGAGAGCGGTAATGATGCGGCGGACGGTGGTAAAGGGGACCGGAGAATGACCAACCCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTa                                                                               <  2:299827/137‑1 (MQ=255)
                                                                                                  gggTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCAGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCa                                           >  8:76153/1‑149 (MQ=255)
                                                                                                       aTGGTGGTAAAGGTGAAAGGACAAGGACCAAACCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTc                                      <  6:170182/149‑1 (MQ=255)
                                                                                                         ggtggtAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAg                                    >  6:139468/1‑149 (MQ=255)
                                                                                                             gtAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGATGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGa                                >  1:189083/1‑149 (MQ=255)
                                                                                                              tAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGAc                               >  5:300669/1‑149 (MQ=255)
                                                                                                               aaaGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACt                              >  8:75059/1‑149 (MQ=255)
                                                                                                                     gAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCt                          <  1:270850/147‑1 (MQ=255)
                                                                                                                     gAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCt                          >  2:270850/1‑147 (MQ=255)
                                                                                                                      aaaGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTg                       <  6:300669/149‑1 (MQ=255)
                                                                                                                      aaaGGAGAATGAGAAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAACGGCTATCGAACTACGGTCGCTTCTCGTCCAAAGAATGGTAGGAATCACCCGCCCGCACTAAACATCACTAATAGAACTTTCAGGTGACTGCCtttt                       >  2:218257/1‑148 (MQ=255)
                                                                                                                       aaGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTg                       >  6:50535/1‑148 (MQ=255)
                                                                                                                         ggAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACt                              >  1:161797/1‑139 (MQ=255)
                                                                                                                         ggAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACt                              <  2:161797/139‑1 (MQ=255)
                                                                                                                           agaATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATaa                  <  1:218257/149‑1 (MQ=255)
                                                                                                                              ccccctgaaGCCCGCCCATCGGCGGGCTTTAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTTAGGTGACTGCCTTTGGATAAGtt               >  2:138537/8‑149 (MQ=255)
                                                                                                                               tGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCCCCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGTTg              >  4:262527/1‑149 (MQ=255)
                                                                                                                                 agcaccccccGCCGTTGCGCGGGCCTCAGGGGGGCGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGTTGt             <  8:295076/141‑1 (MQ=255)
                                                                                                                                  gCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCttt                        >  5:80528/1‑136 (MQ=255)
                                                                                                                                  gCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCttt                        <  6:80528/136‑1 (MQ=255)
                                                                                                                                  gCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGTTGTAt           >  2:50290/1‑149 (MQ=255)
                                                                                                                                  ctgaaGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGTTGTAt           >  5:253288/4‑149 (MQ=255)
                                                                                                                                  ctgaaGCCCGCCCATCGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCCCCGAAAGGTAGGAAGCCCCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGTTGTAt           >  5:281449/4‑149 (MQ=255)
                                                                                                                                  ctgaaGCCCGCCCATCGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGTTGTAt           >  2:117194/4‑149 (MQ=255)
                                                                                                                                      aGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGTTGTATCCAg       <  5:50535/149‑1 (MQ=255)
                                                                                                                                      aGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTGCTCGTCCACCGAAAGGTAGGAAGCCCCCGCCCGCACTACTCATCAGGAATAGCACATTCAGGTGACTGCCTTTGGATAAGATGTATCCAg       >  3:14815/1‑149 (MQ=255)
                                                                                                                                          cgccgATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGTTGTATCCAGCCTg   <  5:139468/149‑1 (MQ=255)
                                                                                                                                           gcccATCGGCGGGCTTTAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTTAGGTGACTGCCTTTGGATAAGTTGTATCCAGCCTGt  <  1:138537/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                 
CGAATGGGCATTTTGGTCTATCCGGCGGAGAGTGAATGTCGAAGCCTGCTTCCCGGTTACGTCGGCTCCAATAAGGTGCGGGAGAGCGGTAATGATGGGGGTGATGGTGGTAAAGGTGAAAGGAGAATGAGCAAAGCCCGCCGATGGGCGGGCTTCAGGGGGGGGCAGAAGGGCTATCGTACTACGGTCGCTTCTCGTCCACCGAAAGGTAGGAAGCACCCGCCCGCACTCCTCATCAGGAATCGCACTTTCAGGTGACTGCCTTTGGATAAGTTGTATCCAGCCTGT  >  NC_002947/1274942‑1275229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑