Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,284,338 T→G 7.0% G92G (GGT→GGG)  PP_1121 → OmpA family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,284,3380TG 7.0% 163.3 / 2.3 54G92G (GGT→GGG) PP_1121OmpA family protein
Reads supporting (aligned to +/- strand):  ref base T (23/27);  new base G (2/2);  total (25/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATCGAAAGCTCCAGCTGGGCTGGCTGGGGTGCGTTGCTGGGCGGTGGCCTGGCGGCGGGCTATTGCTGGGCCCATGGCGATGGCGACGAGGATGGTGACGGCGTGCCAGACAGCCGTGACAAGTGCCCTGGCACCCCGCGTGGTGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTCGAAGAAGTGGTGGTGCAGAAGGAAGAAGTCATTGTCATCCGCGATGTGCACTTCGAGTTCGATTCTGCGCGCCTGACGGCCAGTGACAAAGAGCGCCT  >  NC_002947/1284194‑1284484
                                                                                                                                                |                                                                                                                                                  
cATCGAAAGCTCCAGCTGGGCTGGCTGGGGTGCGTTGCTGGGCGGTGGCCTGGCGGCGGGCTATTGCTGGGCCCATGGCGATGGCGACGAGGATGGTGACGGCGTGCCAGACAGCCGTGACAAGTGCCCTGGCACCCCGCGTGGTGTGc                                                                                                                                                <  2:60015/149‑1 (MQ=255)
      aaGCTCCAGCTGGGCTGGCTGGGGTGCGTTGCTGGGCGGTGGCCTGGCGGCGGGCTATTGCTGGGCCCATGGCGATGGCGACGAGGATGGTGACGGCGTGCCAGACAGCCGTGACAAGTGCCCTGGCACCCCGCGTGGTGTGCAGGTc                                                                                                                                           >  6:151528/1‑148 (MQ=255)
            cAGCTGGGCTGGCTGGGGTGCGTTGCTGGGCGGTGGCCTGGCGGCGGGCTATTGCTGGGCCCATGGCGATGGCGACGAGGATGGTGACGGCGTGCCAGACAGCCGTGACAAGTGCCCTGGCACCCCGCGTGGTGTGCAGGTCGATGcc                                                                                                                                     >  2:199064/1‑148 (MQ=255)
             aGCTGGGCTGGCTGGGGTGCGTTGCTGGGCGGTGGCCTGGCGGCGGGCTATTGCTGGGCCCATGGCGATGGCGACGAGGATGGTGACGGCGTGCCAGACAGCCGTGACAAGTGCCCTGGCACCCCGCGTGGTGTGCAGGTCGATGCCaa                                                                                                                                   <  8:283413/149‑1 (MQ=255)
                       gctggGGTGCGTTGCTGGGCGGTGGCCTGGCGGCGGGCTATTGCTGGGCCCATGGCGATGGCGACGAGGATGGTGACGGCGTGCCAGACAGCCGTGACAAGTGCCCTGGCACCCCGCGTGGTGTGCAGGTCGATGCCAACGGATGccc                                                                                                                          <  1:293830/148‑1 (MQ=255)
                          ggggTGCGTTGCTGGGCGGTGGCCTGGCGGCGGGCTATTGCTGGGCCCATGGCGATGGCGACGAGGATGGTGACGGCGTGCCAGACAGCCGTGACAAGTGCCCTGGCACCCCGCGTGGTGTGCAGGTCGATGCCAACGGATGCCCGCCt                                                                                                                      >  8:212319/1‑149 (MQ=255)
                                        ggCGGTGGCCTGGCGGCGGGCTATTGCTGGGCCCATGGCGATGGCGACGAGGATGGTGACGGCGTGCCAGACAGCCGTGACAAGTGCCCTGGCACCCCGCGTGGTGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCgg                                                                                                         <  1:199064/148‑1 (MQ=255)
                                        ggCGGTGGCCTGGCGGCGGGCTATTGCTGGGCCCATGGCGATGGCGACGAGGATGGTGACGGCGTGCCAGACAGCCGTGACAAGTGCCCTGGCACCCCGCGTGGTGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCg                                                                                                          <  5:4666/147‑1 (MQ=255)
                                            gTGGCCTGGCGGCGGGCTATTGCTGGGCCCATGGCGATGGCGACGAGGATGGTGACGGCGTGCCAGACAGCCGTGACAAGTGCCCTGGCACCCCGCGTGGTGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTc                                                                                                    <  5:122357/149‑1 (MQ=255)
                                                  tGGCGGCGGGCTATTGCTGGGCCCATGGCGATGGCGACGAGGATGGTGACGGCGTGCCAGACAGCCGTGACAAGTGCCCTGGCACCCCGCGTGGTGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTCgaa                                                                                                 >  7:319852/1‑146 (MQ=255)
                                                  tGGCGGCGGGCTATTGCTGGGCCCATGGCGATGGCGACGAGGATGGTGACGGCGTGCCAGACAGCCGTGACAAGTGCCCTGGCACCCCGCGTGGTGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTCgaa                                                                                                 <  8:319852/146‑1 (MQ=255)
                                                    gcggcgGGCTATTGCTGGGCCCATGGCGATGGCGACGAGGATGGTGACGGCGTGCCAGACAGCCGTGACAAGTGCCCTGGCACCCCGCGTGGTGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTCGaagaag                                                                                             <  7:212319/148‑1 (MQ=255)
                                                         gggCTATTGCTGGGCCCATGGCGATGGCGACGAGGATGGTGACGGCGTGCCAGACAGCCGTGACAAGTGCCCTGGCACCCCGCGTGGTGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTCGAAGAAgtggtgg                                                                                       >  3:13073/1‑149 (MQ=255)
                                                          ggCTATTGCTGGGCCCATGGCGATGGCGACGAGGATGGTGACGGCGTGCCAGACAGCCGTGACAAGTGCCCTGGCACCCCGCGTGGTGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTCGAAGAAgtggtggt                                                                                      <  3:298484/149‑1 (MQ=255)
                                                                 gCTGGGCCCATGGCGATGGCGACGAGGATGGTGACGGCGTGCCAGACAGCCGTGACAAGTGCCCTGGCACCCCGCGTGGTGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTCGAAGAAGTGGTGGTGCAgaag                                                                               >  8:22071/1‑149 (MQ=255)
                                                                 gCTGGGCCCATGGCGATGGCGACGAGGATGGTGACGGCGTGCCAGACAGCCGTGACAAGTGCCCTGGCACCCCGCGTGGTGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTCGAAGAAGTGGTGGTGCAgaa                                                                                <  5:151528/148‑1 (MQ=255)
                                                                  cTGGGCCCATGGCGATGGCGACGAGGATGGTGACGGCGTGCCAGACAGCCGTGACAAGTGCCCTGGCACCCCGCGTGGTGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTCGAAGAAGTGGTGGTGCAgaag                                                                               <  7:22071/148‑1 (MQ=255)
                                                                    gggCCCATGGCGATGGCGACGAGGATGGTGACGGCGTGCCAGACAGCCGTGACAAGTGCCCTGGCACCCCGCGTGGTGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTCGAAGAAGTGGTGGTGCAgaagg                                                                              >  5:160935/1‑147 (MQ=255)
                                                                     ggCCCATGGCGATGGCGACGAGGATGGTGACGGCGTGCCAGACAGCCGTGACAAGTGCCCTGGCACCCCGCGTGGTGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTCGAAGAAGTGGTGGTGCAgaaggaa                                                                            <  6:17279/148‑1 (MQ=255)
                                                                      gCCCATGGCGATGGCGACGAGGATGGTGACGGCGTGCCAGACAGCCGTGACAAGTGCCCTGGCACCCCGCGTGGTGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTCGAAGAAGTGGTGGTGCAgaaggaag                                                                           >  3:324360/1‑148 (MQ=255)
                                                                       cccATGGCGATGGCGACGAGGATGGTGACGGCGTGCCAGACAGCCGTGACAAGTGCCCTGGCACCCCGCGTGGTGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTCGAAGAAGTGGTGGTGCAGAAGgaagaa                                                                         <  7:222880/149‑1 (MQ=255)
                                                                        ccATTGCGATGGCGACGAGGATGGTGACGGCGTGCCAGACAGCCGTGACAAGTGCCCTGGCACCCCGCGTGGTGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCg                                                                                                                >  7:241716/1‑109 (MQ=255)
                                                                        ccATTGCGATGGCGACGAGGATGGTGACGGCGTGCCAGACAGCCGTGACAAGTGCCCTGGCACCCCGCGTGGTGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCg                                                                                                                <  8:241716/109‑1 (MQ=255)
                                                                          atggcgatggcgACGAGGATGGTGACGGCGTGCCAGACAGCCGTGACAAGTGCCCTGGCACCCCGCGTGGTGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTCGATGAAGTGGTGGTGCAGAAGGAAGAAGTc                                                                      <  1:16633/149‑1 (MQ=255)
                                                                           tggcgatggcgaCGAGGATGGTGACGGCGTGCCAGACAGCCGTGACAAGTGCCCTGGCACCCCGCGTGGTGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTCGAAGAAGTGGTGGTGCAGAAGGAAGAAGTCa                                                                     >  6:28223/1‑149 (MQ=255)
                                                                           tggcgatggcgaCGAGGATGGTGACGGCGTGCCAGACAGCCGTGACAAGTGCCCTGGCACCCCGCGTGGTGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTCGAAGAAGTGGTGGTGCAGAAGGAAGAAGTCa                                                                     >  8:63944/1‑149 (MQ=255)
                                                                                atggcgaCGAGGATGGTGACGGCGTGCCAGACAGCCGTGACAAGTGCCCTGGCACCCCGCGTGGTGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTCGAAGAAGTGGTGGTGCAGAAGGAAGAAGTCATTGTc                                                                <  5:28223/149‑1 (MQ=255)
                                                                                   gcgaCGAGGATGGTGACGGCGTGCCAGACAGCCGTGACAAGTGCCCTGGCACCCCGCGTGGTGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTCGaagaag                                                                                             <  6:13799/117‑1 (MQ=255)
                                                                                   gcgaCGAGGATGGTGACGGCGTGCCAGACAGCCGTGACAAGTGCCCTGGCACCCCGCGTGGTGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTCGaagaag                                                                                             >  5:13799/1‑117 (MQ=255)
                                                                                     gacgaGGATGGTGACGGCGTGCCAGACAGCCGTGACAAGTGCCCTGGCACCCCGCGTGGTGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTCGAAGAAGTGGTGGTGCAGAAGGAAGAAGTCAt                                                                    <  1:131932/140‑1 (MQ=255)
                                                                                     gacgaGGATGGTGACGGCGTGCCAGACAGCCGTGACAAGTGCCCTGGCACCCCGCGTGGTGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTCGAAGAAGTGGTGGTGCAGAAGGAAGAAGTCAt                                                                    >  2:131932/1‑140 (MQ=255)
                                                                                     gacgaGGATGGTGACGGCGTGCCAGACAGCCGTGACAAGTGCCCTGGCACCCCGCGTGGTGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTCGAAGAAGTGGTGGTGCAGAAGGAAGAAGTCATTGTCATCcg                                                           >  5:15062/1‑149 (MQ=255)
                                                                                      acgaGGATGGTGACGGCGTGCCAGACAGCCGTGACAAGTGCCCTGGCACCCCGCGTGGTGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTCGAAGAAGTGGTGGTGCAGAAGGAAGAAGTCATTGTCATCcgc                                                          >  6:934/1‑149 (MQ=255)
                                                                                           gATGGTGACGGCGTGCCAGACAGCCGTGACAAGTGCCCTGGCACCCCGCGTGGTGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTCGAAGAAGTGGTGGTGCAGAAGGAAGAAGTCATTGTCATCCGCGAtgt                                                     >  3:297220/1‑149 (MQ=255)
                                                                                             tGGTGACGGCGTGCCAGACAGCCGTGACAAGTGCCCTGGCACCCCGCGTGGTGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTCGAAGAAGTGGTGGTGCAGAAGGAAGAAGTCATTGTCATCCGCGAtgtg                                                    >  8:294624/1‑148 (MQ=255)
                                                                                                      cGTGCCAGACAGCCGTGACAAGTGCCCTGGCACCCCGCGTGGTGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTCGAAGAAGTGGTGGTGCAGAAGGAAGAAGTCATTGTCATCCGCGATGTGCACTTCGAGt                                          <  6:230007/149‑1 (MQ=255)
                                                                                                       gTGCCAGACAGCCGTGACAAGTGCCCTGGCACCCCGCGTGGTGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTCGAAGAAGTGGTGGTGCAgaag                                                                               >  1:235707/1‑111 (MQ=255)
                                                                                                       gTGCCAGACAGCCGTGACAAGTGCCCTGGCACCCCGCGTGGTGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTCGAAGAAGTGGTGGTGCAgaag                                                                               <  2:235707/111‑1 (MQ=255)
                                                                                                          ccAGACAGCCGTGACAAGTGCCCTGGCACCCCGCGTGGTGTGCAGGTCGATGTCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTCGAAGAAGTGGTGGTGCAGAAGGAAGAAGTCATTGTCATCCGCGATGTGCACTTCGAGTTCGa                                      >  5:38793/1‑149 (MQ=255)
                                                                                                          ccAGACAGCCGTGACAAGTGCCCTGGCACCCCGCGTGGTGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTCGAAGAAGTGGTGGTGCAGAAGGAAGAAGTCATTGTCATCCGCGATGTGCACTTCGAGTTCg                                       <  4:324360/148‑1 (MQ=255)
                                                                                                           cAGACAGCCGTGACAAGTGCCCTGGCACCCCGCGTGGTGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTCGAAGAAGTGGTGGAGCAGAAGGAAGAAGTCATTGTCATCCGCGATGTGCACTTCGAGTTCGAt                                     >  5:97928/1‑149 (MQ=255)
                                                                                                                       aCAAGTGCCCTGGCACCCCGCGTGGTGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTCGAAGAAGTGGTGGTGCAGAAGGAAGAAGTCATTGTCATCCGCGATGTGCACTTCGAGTTCGATTCTGCGCGCCt                          >  4:295263/1‑148 (MQ=255)
                                                                                                                       aCAAGTGCCCTGGCACCCCGCGTGGTGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTCGAAGAAGTGGTGGTGCAGAAGGAAGAAGTCATTGTCATCCGCGATGTGCACTTCGAGTTCGATTCTGCGCGCCt                          <  4:13073/148‑1 (MQ=255)
                                                                                                                         aaGTGCCCTGGCACCCCGCGTGGTGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTCGAAGAAGTGGTGGTGCAGAAGGAAGAAGTCATTGTCATCCGCGATGTGCACTTCGAGTTCGATTCTGCGCGCCTGAc                       >  2:300017/1‑149 (MQ=255)
                                                                                                                              cccTGGCACCCCGCGTGGTGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTCGAAGAAGTGGTGGTGCAGAAGGAAGAAGTCATTGTCATCCGCGATGTGCACTTCGAGTTCGATTCTGCGCGCCTGACGGCCa                  <  7:63944/149‑1 (MQ=255)
                                                                                                                                cTGGCACCCCGCGTGGTGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTCGAAGAAGTGGTGGTGCAGAAGGAAGAAGTCATTGTCATCCGCGATGTGCACTTCGAGTTCGATTCTGCGCGCCTGACGGCCAGt                <  6:97928/149‑1 (MQ=255)
                                                                                                                                 ctgCACACCACGCGGGGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTCGAAGAAGTGGTGGTGCAGAAGGAAGAAGTCATTGTCATCCGCGATGTGCACTTCGAGTTCGATTCTGCGCGCCTGACGGCCAGt                >  6:183911/3‑148 (MQ=255)
                                                                                                                                  tgCACACCACGCGGGGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTCGAAGAAGTGGTGGTGCAGAAGGAAGAAGTCATTGTCATCCGCGATGTGCACTTCGa                                            >  6:209868/2‑119 (MQ=255)
                                                                                                                                  tgCACACCACGCGGGGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTCGAAGAAGTGGTGGTGCAGAAGGAAGAAGTCATTGTCATCCGCGATGTGCACTTCGa                                            <  5:209868/118‑1 (MQ=255)
                                                                                                                                   gCACCCCGCGTGGTGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTCGAAGAAGTGGTGGTGCAGAAGGAAGAAGTCATTGTCATCCGCGATGTGCACTTCGAGTTCGATTCTGCGCGCCTGACGGCCAGTGa              <  7:294624/148‑1 (MQ=255)
                                                                                                                                    cacaCCACGCGGGGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTCGAAGAAGTGGTGGTGCAGAAGGAAGAAGTCATTGTCATCCGCGATGTGCACTTCGAGTTCGATTCTGCGCGCCTGACGGCCAGTGAc             <  2:227807/148‑1 (MQ=255)
                                                                                                                                      ccccGCGTGGTGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTCGAAGAAGTGGTGGTGCAGAAGGAAGAAGTCATTGTCATCCGCGATGTGCACTTCGAGTTCGATTCTGCGCGCCTGACGGCCAGTGACaaa          <  5:934/149‑1 (MQ=255)
                                                                                                                                         cgcgTGGTGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTCGAAGAAGTGGTGGTGCAGAAGGAAGAAGTCATTGTCATCCGCGATGTGCACTTCGAGTTCGATTCTGCGCGCCTGACGGCCAGTGACAAagag       >  2:141573/1‑149 (MQ=255)
                                                                                                                                         cgcgTGGTGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTCGAAGAAGTGGTGGTGCAGAAGGAAGAAGTCATTGTCATCCGCGATGTGCACTTCGAGTTCGATTCTGCGCGCCTGACGGCCAGTGACAAagag       <  6:15062/149‑1 (MQ=255)
                                                                                                                                              ggtgTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTCGAAGAAGTGGTGGTGCAGAAGGAAGAAGTCATTGTCATCCGCGATGTGCACTTCGAGTTCGATTCTGCGCGCCTGACGGCCAGTGACAAAGAGCGCCt  <  3:295263/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
CATCGAAAGCTCCAGCTGGGCTGGCTGGGGTGCGTTGCTGGGCGGTGGCCTGGCGGCGGGCTATTGCTGGGCCCATGGCGATGGCGACGAGGATGGTGACGGCGTGCCAGACAGCCGTGACAAGTGCCCTGGCACCCCGCGTGGTGTGCAGGTCGATGCCAACGGATGCCCGCCTGAGCCGGTTGCGGTGGTCGAAGAAGTGGTGGTGCAGAAGGAAGAAGTCATTGTCATCCGCGATGTGCACTTCGAGTTCGATTCTGCGCGCCTGACGGCCAGTGACAAAGAGCGCCT  >  NC_002947/1284194‑1284484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑