Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,352,197 G→C 5.3% intergenic (‑413/+29) nrdB ← / ← PP_1178 ribonucleoside‑diphosphate reductase subunit beta/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,352,1970GC 5.3% 242.7 / 3.4 75intergenic (‑413/+29)nrdB/PP_1178ribonucleoside‑diphosphate reductase subunit beta/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (36/35);  new base C (2/2);  total (38/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.81e-01

CGGTTTGGCGTGCGTCCGTTCACGAAGCGGGTGGCTTCTTCTACGGGGGGAGAGTGTAGCGCCAACCTATATGTTGTGCCAGTTTAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGT  >  NC_002947/1352049‑1352343
                                                                                                                                                    |                                                                                                                                                  
cGGTTTGGCGTGCGTCCGTTCACGAAGCGGGTGGCTTCTTCTACGGGGGGAGAGTGTAGCGCCAACCTATATGTTGTGCCAGTTTAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTg                                                                                                                                                    >  5:197018/1‑149 (MQ=255)
cGGTTTGGCGTGCGTCCGTTCACGAAGCGGGTGGCTTCTTCTACGGGGGGAGAGTGTAGCGCCAACCTATATGTTGTGCCAGTTTAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTg                                                                                                                                                    >  2:17318/1‑149 (MQ=255)
 ggTTTGGCGTGCGTCCGTTCACGAAGCGGGTGGCTTCTTCTACGGGGGGAGAGTGTAGCGCCAACCTATATGTTGTGCCAGTTTAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTgg                                                                                                                                                   <  4:268525/149‑1 (MQ=255)
         gtgcgtCCGTTGACGAAGCGGGTGGCTTCTTCTACGGGGGGAGAGTGTAGCGCCAACCTATATGTTGTGCCAGTTTAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCg                                                                                                                                           <  2:229449/149‑1 (MQ=255)
               ccGTTCACGAAGCGGGTGGCTTCTTCTACGGGGGGAGAGTGTAGCGCCAACCTATATGTTGTGCCAGTTTAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGcc                                                                                                                                            >  6:294190/1‑142 (MQ=255)
               ccGTTCACGAAGCGGGTGGCTTCTTCTACGGGGGGAGAGTGTAGCGCCAACCTATATGTTGTGCCAGTTTAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGcc                                                                                                                                            <  5:294190/142‑1 (MQ=255)
                 gTTCACGAAGCGGGTGGCTTCTTCTACGGGGGGAGAGTGTAGCGCCAACCTATATTTTGTGCCAGTTTAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAg                                                                                                                                   <  7:276221/149‑1 (MQ=255)
                     aCGAAGCGGGTGGCTTCTTCTACGGTGGGAGAGTGTAGCGCCAACCTATATGTTGTGCCAGTTTAAATTTGAAGCCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCATCGCCGGCAAGCCCACGGTGAGgcc                                                                                                                                <  7:316465/148‑1 (MQ=255)
                             ggTGGCTTCTTCTACGGGGGGAGAGTGTAGCGCCAACCTATATGTTGTGCCAGTTTAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCcaccac                                                                                                                       <  1:17318/149‑1 (MQ=255)
                                  cttcttCTACGGGGGGAGAGTGTAGCGCCAACCTATATGTTGTGCCAGTTTAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCATCGCCGGCAAGCCCACGGTGAGgcc                                                                                                                                >  1:23318/1‑135 (MQ=255)
                                  cttcttCTACGGGGGGAGAGTGTAGCGCCAACCTATATGTTGTGCCAGTTTAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCATCGCCGGCAAGCCCACGGTGAGgcc                                                                                                                                <  2:23318/135‑1 (MQ=255)
                                   ttcttcTACGGGGGGAGAGTGTAGCGCCAACCTATATGTTGTGCCAGTTTAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTa                                                                                                                 >  7:255262/1‑149 (MQ=255)
                                        ctACGGGGGGAGAGTGTAGCGCCAACCTATATGTTGTGCCAGTTTAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACggtg                                                                                                            >  8:4007/1‑149 (MQ=255)
                                          aCGGGGGGAGAGTGTAGCGCCAACCTATATGTTGTGCCAGTTTAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACggtggg                                                                                                          >  7:270347/1‑149 (MQ=255)
                                           cGGGGGGAGAGTGTAGCGCCAACCTATATGTTGTGCCAGTTTAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACggtgg                                                                                                           <  4:180519/147‑1 (MQ=255)
                                                  agagTTTAGCGCCAACCTATATGTTGTGCCAGTTTAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGc                                                                                                  <  3:198505/149‑1 (MQ=255)
                                                             ccAACCTATATGTTGTGCCAGTTTAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAAt                                                                                       <  7:344339/149‑1 (MQ=255)
                                                                   tataTGTTGTGCCAGTTTAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATgtgtgt                                                                                 >  6:27985/1‑149 (MQ=255)
                                                                        gttgtGCCAGTTTAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCTTTTCGACAATGTGTGTCTTgg                                                                            >  6:42570/1‑149 (MQ=255)
                                                                         ttgtGCCAGTTTAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTggg                                                                           >  5:335276/1‑149 (MQ=255)
                                                                             gCCAGTTTAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACggtgg                                                                                                           <  6:10429/113‑1 (MQ=255)
                                                                               cAGTTTAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGACGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCtt                                                                     >  5:65389/1‑149 (MQ=255)
                                                                                aGTTTAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTg                                                                    >  2:320413/1‑149 (MQ=255)
                                                                                 gTTTAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGc                                                                   >  8:207418/1‑149 (MQ=255)
                                                                                  tttAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCg                                                                  >  5:357640/1‑149 (MQ=255)
                                                                                  tttAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCg                                                                  >  7:325758/1‑149 (MQ=255)
                                                                                   ttAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGt                                                                 >  2:310845/1‑149 (MQ=255)
                                                                                        tttGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGgcca                                                                                                                               <  1:295319/82‑1 (MQ=255)
                                                                                        tttGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGgcca                                                                                                                               >  2:295319/1‑82 (MQ=255)
                                                                                             aGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCa                                                       >  3:19501/1‑149 (MQ=255)
                                                                                             aGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCa                                                       >  6:356921/1‑149 (MQ=255)
                                                                                             aGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCa                                                       <  5:27985/149‑1 (MQ=255)
                                                                                                cacaTCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCg                                                    >  4:338240/1‑149 (MQ=255)
                                                                                                 acaTCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCAcc                                                     >  3:244123/1‑147 (MQ=255)
                                                                                                  catcatATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAg                                                  <  3:338240/149‑1 (MQ=255)
                                                                                                  catcatATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAg                                                  >  5:368528/1‑149 (MQ=255)
                                                                                                  catcatATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAg                                                  <  6:357640/149‑1 (MQ=255)
                                                                                                  catcatATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAg                                                  <  6:197018/149‑1 (MQ=255)
                                                                                                    tcatATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGt                                                >  7:247133/1‑149 (MQ=255)
                                                                                                     catATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGACTGTTCCACCGAGGt                                                >  8:262404/1‑148 (MQ=255)
                                                                                                      atatGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGt                                                           <  6:309036/136‑1 (MQ=255)
                                                                                                      atatGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGt                                                           >  5:309036/1‑136 (MQ=255)
                                                                                                      atatGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTg                                               >  2:344384/1‑148 (MQ=255)
                                                                                                       tatGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCt                                                             >  1:281317/1‑133 (MQ=255)
                                                                                                       tatGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCt                                                             <  2:281317/133‑1 (MQ=255)
                                                                                                         tGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGaaa                                            <  5:42570/148‑1 (MQ=255)
                                                                                                         tGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAAc                                           <  7:151713/149‑1 (MQ=255)
                                                                                                          ggTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACg                                          >  2:127155/1‑149 (MQ=255)
                                                                                                              tttCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGcc                                       <  8:325758/148‑1 (MQ=255)
                                                                                                              tttCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGcc                                       <  6:65389/148‑1 (MQ=255)
                                                                                                              tttCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCg                                      >  2:8685/1‑149 (MQ=255)
                                                                                                                     aGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCt                                >  6:149351/1‑148 (MQ=255)
                                                                                                                     aGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCt                                <  8:247133/148‑1 (MQ=255)
                                                                                                                     aGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCt                                <  2:63462/148‑1 (MQ=255)
                                                                                                                     aGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCt                                <  5:149351/148‑1 (MQ=255)
                                                                                                                      ggCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCTGTTTCTg                               <  6:335276/148‑1 (MQ=255)
                                                                                                                         ttGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTc                                                         <  3:154779/119‑1 (MQ=255)
                                                                                                                         ttGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTc                                                         >  4:154779/1‑119 (MQ=255)
                                                                                                                         ttGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGa                                                   >  8:87470/1‑125 (MQ=255)
                                                                                                                         ttGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGa                                                   <  7:87470/125‑1 (MQ=255)
                                                                                                                         ttGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGtt                           >  1:75800/1‑149 (MQ=255)
                                                                                                                         ttGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGtt                           >  5:46779/1‑149 (MQ=255)
                                                                                                                         ttGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGtt                           >  3:343798/1‑149 (MQ=255)
                                                                                                                            tgtTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGc                         <  1:344384/148‑1 (MQ=255)
                                                                                                                              ttGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGa                       <  6:46779/148‑1 (MQ=255)
                                                                                                                              ttGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGAc                      >  2:205626/1‑149 (MQ=255)
                                                                                                                              ttGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGAc                      <  6:368528/149‑1 (MQ=255)
                                                                                                                              ttGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGAc                      <  8:270347/149‑1 (MQ=255)
                                                                                                                                   ggAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATc                 <  7:207418/149‑1 (MQ=255)
                                                                                                                                       tCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGc             <  1:320413/149‑1 (MQ=255)
                                                                                                                                       tCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGc             <  8:255262/149‑1 (MQ=255)
                                                                                                                                       ctggCCTCATCGCCGGCAAGCCCACGGTGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGc             >  3:59066/3‑149 (MQ=255)
                                                                                                                                        cGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCg            <  1:310845/149‑1 (MQ=255)
                                                                                                                                         ggCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCga           <  1:8685/149‑1 (MQ=255)
                                                                                                                                          gCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGc             >  7:150210/1‑146 (MQ=255)
                                                                                                                                            cTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGAt        >  4:138826/1‑149 (MQ=255)
                                                                                                                                             tCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATg       >  3:15276/1‑149 (MQ=255)
                                                                                                                                                  gTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGt  <  4:343798/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CGGTTTGGCGTGCGTCCGTTCACGAAGCGGGTGGCTTCTTCTACGGGGGGAGAGTGTAGCGCCAACCTATATGTTGTGCCAGTTTAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGT  >  NC_002947/1352049‑1352343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑