Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,352,210 A→G 5.2% intergenic (‑426/+16) nrdB ← / ← PP_1178 ribonucleoside‑diphosphate reductase subunit beta/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,352,2100AG 5.2% 233.2 / 2.2 76intergenic (‑426/+16)nrdB/PP_1178ribonucleoside‑diphosphate reductase subunit beta/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (37/35);  new base G (2/2);  total (39/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.90e-01

GTTCACGAAGCGGGTGGCTTCTTCTACGGGGGGAGAGTGTAGCGCCAACCTATATGTTGTGCCAGTTTAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGC  >  NC_002947/1352066‑1352354
                                                                                                                                                |                                                                                                                                                
gTTCACGAAGCGGGTGGCTTCTTCTACGGGGGGAGAGTGTAGCGCCAACCTATATTTTGTGCCAGTTTAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAg                                                                                                                                              <  7:276221/149‑1 (MQ=255)
    aCGAAGCGGGTGGCTTCTTCTACGGTGGGAGAGTGTAGCGCCAACCTATATGTTGTGCCAGTTTAAATTTGAAGCCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCATCGCCGGCAAGCCCACGGTGAGgcc                                                                                                                                           <  7:316465/148‑1 (MQ=255)
            ggTGGCTTCTTCTACGGGGGGAGAGTGTAGCGCCAACCTATATGTTGTGCCAGTTTAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCcaccac                                                                                                                                  <  1:17318/149‑1 (MQ=255)
                 cttcttCTACGGGGGGAGAGTGTAGCGCCAACCTATATGTTGTGCCAGTTTAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCATCGCCGGCAAGCCCACGGTGAGgcc                                                                                                                                           >  1:23318/1‑135 (MQ=255)
                 cttcttCTACGGGGGGAGAGTGTAGCGCCAACCTATATGTTGTGCCAGTTTAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCATCGCCGGCAAGCCCACGGTGAGgcc                                                                                                                                           <  2:23318/135‑1 (MQ=255)
                  ttcttcTACGGGGGGAGAGTGTAGCGCCAACCTATATGTTGTGCCAGTTTAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTa                                                                                                                            >  7:255262/1‑149 (MQ=255)
                       ctACGGGGGGAGAGTGTAGCGCCAACCTATATGTTGTGCCAGTTTAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACggtg                                                                                                                       >  8:4007/1‑149 (MQ=255)
                         aCGGGGGGAGAGTGTAGCGCCAACCTATATGTTGTGCCAGTTTAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACggtggg                                                                                                                     >  7:270347/1‑149 (MQ=255)
                          cGGGGGGAGAGTGTAGCGCCAACCTATATGTTGTGCCAGTTTAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACggtgg                                                                                                                      <  4:180519/147‑1 (MQ=255)
                                 agagTTTAGCGCCAACCTATATGTTGTGCCAGTTTAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGc                                                                                                             <  3:198505/149‑1 (MQ=255)
                                            ccAACCTATATGTTGTGCCAGTTTAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAAt                                                                                                  <  7:344339/149‑1 (MQ=255)
                                                  tataTGTTGTGCCAGTTTAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATgtgtgt                                                                                            >  6:27985/1‑149 (MQ=255)
                                                       gttgtGCCAGTTTAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCTTTTCGACAATGTGTGTCTTgg                                                                                       >  6:42570/1‑149 (MQ=255)
                                                        ttgtGCCAGTTTAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTggg                                                                                      >  5:335276/1‑149 (MQ=255)
                                                            gCCAGTTTAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACggtgg                                                                                                                      <  6:10429/113‑1 (MQ=255)
                                                              cAGTTTAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGACGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCtt                                                                                >  5:65389/1‑149 (MQ=255)
                                                               aGTTTAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTg                                                                               >  2:320413/1‑149 (MQ=255)
                                                                gTTTAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGc                                                                              >  8:207418/1‑149 (MQ=255)
                                                                 tttAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCg                                                                             >  7:325758/1‑149 (MQ=255)
                                                                 tttAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCg                                                                             >  5:357640/1‑149 (MQ=255)
                                                                  ttAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGt                                                                            >  2:310845/1‑149 (MQ=255)
                                                                       tttGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGgcca                                                                                                                                          >  2:295319/1‑82 (MQ=255)
                                                                       tttGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGgcca                                                                                                                                          <  1:295319/82‑1 (MQ=255)
                                                                            aGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCa                                                                  >  6:356921/1‑149 (MQ=255)
                                                                            aGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCa                                                                  >  3:19501/1‑149 (MQ=255)
                                                                            aGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCa                                                                  <  5:27985/149‑1 (MQ=255)
                                                                               cacaTCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCg                                                               >  4:338240/1‑149 (MQ=255)
                                                                                acaTCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCAcc                                                                >  3:244123/1‑147 (MQ=255)
                                                                                 catcatATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAg                                                             <  6:357640/149‑1 (MQ=255)
                                                                                 catcatATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAg                                                             <  6:197018/149‑1 (MQ=255)
                                                                                 catcatATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAg                                                             >  5:368528/1‑149 (MQ=255)
                                                                                 catcatATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAg                                                             <  3:338240/149‑1 (MQ=255)
                                                                                   tcatATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGt                                                           >  7:247133/1‑149 (MQ=255)
                                                                                    catATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGACTGTTCCACCGAGGt                                                           >  8:262404/1‑148 (MQ=255)
                                                                                     atatGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGt                                                                      <  6:309036/136‑1 (MQ=255)
                                                                                     atatGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGt                                                                      >  5:309036/1‑136 (MQ=255)
                                                                                     atatGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTg                                                          >  2:344384/1‑148 (MQ=255)
                                                                                      tatGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCt                                                                        >  1:281317/1‑133 (MQ=255)
                                                                                      tatGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCt                                                                        <  2:281317/133‑1 (MQ=255)
                                                                                        tGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGaaa                                                       <  5:42570/148‑1 (MQ=255)
                                                                                        tGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAAc                                                      <  7:151713/149‑1 (MQ=255)
                                                                                         ggTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACg                                                     >  2:127155/1‑149 (MQ=255)
                                                                                             tttCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGcc                                                  <  8:325758/148‑1 (MQ=255)
                                                                                             tttCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGcc                                                  <  6:65389/148‑1 (MQ=255)
                                                                                             tttCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCg                                                 >  2:8685/1‑149 (MQ=255)
                                                                                                    aGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCt                                           <  2:63462/148‑1 (MQ=255)
                                                                                                    aGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCt                                           >  6:149351/1‑148 (MQ=255)
                                                                                                    aGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCt                                           <  8:247133/148‑1 (MQ=255)
                                                                                                    aGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCt                                           <  5:149351/148‑1 (MQ=255)
                                                                                                     ggCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCTGTTTCTg                                          <  6:335276/148‑1 (MQ=255)
                                                                                                        ttGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTc                                                                    <  3:154779/119‑1 (MQ=255)
                                                                                                        ttGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTc                                                                    >  4:154779/1‑119 (MQ=255)
                                                                                                        ttGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGa                                                              >  8:87470/1‑125 (MQ=255)
                                                                                                        ttGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGa                                                              <  7:87470/125‑1 (MQ=255)
                                                                                                        ttGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGtt                                      >  3:343798/1‑149 (MQ=255)
                                                                                                        ttGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGtt                                      >  5:46779/1‑149 (MQ=255)
                                                                                                        ttGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGtt                                      >  1:75800/1‑149 (MQ=255)
                                                                                                           tgtTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGc                                    <  1:344384/148‑1 (MQ=255)
                                                                                                             ttGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGa                                  <  6:46779/148‑1 (MQ=255)
                                                                                                             ttGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGAc                                 >  2:205626/1‑149 (MQ=255)
                                                                                                             ttGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGAc                                 <  6:368528/149‑1 (MQ=255)
                                                                                                             ttGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGAc                                 <  8:270347/149‑1 (MQ=255)
                                                                                                                  ggAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATc                            <  7:207418/149‑1 (MQ=255)
                                                                                                                      tCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGc                        <  1:320413/149‑1 (MQ=255)
                                                                                                                      tCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGc                        <  8:255262/149‑1 (MQ=255)
                                                                                                                      ctggCCTCATCGCCGGCAAGCCCACGGTGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGc                        >  3:59066/3‑149 (MQ=255)
                                                                                                                       cGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCg                       <  1:310845/149‑1 (MQ=255)
                                                                                                                        ggCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCga                      <  1:8685/149‑1 (MQ=255)
                                                                                                                         gCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGc                        >  7:150210/1‑146 (MQ=255)
                                                                                                                           cTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGAt                   >  4:138826/1‑149 (MQ=255)
                                                                                                                            tCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATg                  >  3:15276/1‑149 (MQ=255)
                                                                                                                                 gTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGt             <  4:343798/149‑1 (MQ=255)
                                                                                                                                    ggCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGtttt          <  4:244123/149‑1 (MQ=255)
                                                                                                                                         gCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCcg     >  7:159522/1‑149 (MQ=255)
                                                                                                                                          ccGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCcgc    <  7:4007/149‑1 (MQ=255)
                                                                                                                                            ggCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCgcgc  >  2:116574/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                
GTTCACGAAGCGGGTGGCTTCTTCTACGGGGGGAGAGTGTAGCGCCAACCTATATGTTGTGCCAGTTTAAATTTGAAGGCACATCATATGGTGTTTCCTGAGGCTTGTGTTGGCGGAGTCGGCCTCACCGTGGGCTTGCCGGCGATGAGGCCAGCCACCACTAGCTACGGTGGGTGTACAGCGTTTCGACAATGTGTGTCTTGGGGTGCTTGCGTGCCTGTTCCACCGAGGTGAAACGGCCGGTTTCTGCGTTGCGACCAATCTTGCGAGATGCCAGTTTTTTCCGCGC  >  NC_002947/1352066‑1352354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑