Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,358,572 T→C 5.9% A187A (GCA→GCG)  PP_1183 ← siderophore biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,358,5720TC 5.9% 201.5 / 2.3 67A187A (GCA→GCG) PP_1183siderophore biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base T (29/34);  new base C (2/2);  total (31/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

GGTCAGACGCTGACCAGGCTCAGCAGGTGGCCGTCCTGCAGGCAGAACTGGCCTTGCAGCTCTGCGCCTTGCTGCCACTGCGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGAGTCAGTGGGTACAGGGTCTTGAACAAGCTTTCCTTCAGCGAAAAGGTCAAGGTAACGGTCAGGCCGAGCTGACGGCGGTCCAGGCGCTCCAGCTCTGCCGGGATAAGGATTTCACC  >  NC_002947/1358429‑1358715
                                                                                                                                               |                                                                                                                                               
ggTCAGACGCTGACCAGGCTCAGCAGGTGGCCGTCCTGCAGGCAGAACTGGCCTTGCAGCTCTGCGCCTTGCTGCCACTGCGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCgtg                                                                                                                                            <  3:196065/149‑1 (MQ=255)
      aCGCTGACCAGGCTCAGCAGGTGGCCGTCCTGCAGGCAGAACTGGCCTTGCAGCTCTGCGCCTTGCTGCCACTGCGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCTTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGaa                                                                                                                                      <  1:60938/149‑1 (MQ=255)
       cGCTGACCAGGCTCAGCAGGTGGCCGTCCTGCAGGCAGAACTGGCCTTGCAGCTCTGCGCCTTGCTGCCACTGCGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAg                                                                                                                                     <  7:339977/149‑1 (MQ=255)
            aCCAGGCTCAGCAGGTGGCCGTCCTGCAGGCAGAACTGGCCTTGCAGCTCTGCGCCTTGCTGCCACTGCGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGTAg                                                                                                                                  >  2:168737/1‑147 (MQ=255)
            aCCAGGCTCAGCACGTGGCCGTCCTGCAGGCAGAACTGGCCTTGCAGCTCTGCGCCTTGCTGCCACTGCGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGACGAGCACATCTGCGTGATCGAAGTAg                                                                                                                                  >  8:166150/1‑147 (MQ=255)
             ccAGGCTCAGCAGGTGGCCGTCCTGCAGGCAGAACTGGCCTTGCAGCTCTGCGCCTTGCTGCCACTGCGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGTAGAAg                                                                                                                               <  6:186003/149‑1 (MQ=255)
                 gCTCAGCAGGTGGCCGTCCTGCAGGGAGAACTGGCCTTGCAGCTCTGCGCCTTGCTGCCACTGCGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGtt                                                                                                                           <  6:5642/149‑1 (MQ=255)
                 gCTCAGCAGGTGGCCGTCCTGCAGGCAGAACTGGCCTTGCAGCTCTGCGCCTTGCTGCCACTGCGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGGACTTCTGCGTGTTCGAAGTAGAAGCGtt                                                                                                                           <  6:10982/149‑1 (MQ=255)
                 gCTCAGCAGGTGGCCGTCCTGCAGGCAGAACTGGCCTTGCAGCTCTGCGCCTTGCTGCCACTGCGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCCGCGTGTTCGAAGTAGAAGCGtt                                                                                                                           >  3:367512/1‑149 (MQ=255)
                 gCTCAGCAGGTGGCCGTCCTGCAGGCAGAACTGGCCTTGCAGCTCTGCGCCTTGCTGCCACTGCGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCCGCGTGTTCGAAGTAGAAGCGtt                                                                                                                           >  3:361805/1‑149 (MQ=255)
                  cTCAGCAGGTGGCCGTCCTGCAGGCAGAACTGGCCTTGCAGCTCTGCGCCTTGCTGCCACTGCGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTg                                                                                                                          <  1:6306/149‑1 (MQ=255)
                   tCAGCAGGTGGCCGTCCTGCAGGCAGAACTGGCCTTGCAGCTCTGCGCCTTGCTGCCACTGCGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTgg                                                                                                                         >  7:1121/1‑149 (MQ=255)
                        agGTGGCCGTCCTGCAGGCAGAACTGGCCTTGCAGCTCTGCGCCTTGCTGCCACTGCGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGAGt                                                                                                                    <  1:168737/149‑1 (MQ=255)
                          gTGGCCGTCCTGCAGGCAGAACTGGCCTTGCAGCTCTGCGCCTTGCTGCCACTGCGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACAAGTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGAGt                                                                                                                    >  8:266952/1‑147 (MQ=255)
                             gCCGTCCTGCAGGCAGAACTGGCCTTGCAGCTCTGCGCCTTGCTGCCACTGCGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGAGTCAGt                                                                                                                <  8:311594/148‑1 (MQ=255)
                             gCCGTCCTGCAGGCAGAACTGGCCTTGCAGCTCTGCGCCTTGCTGCCACTGCGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGAGTCAGTg                                                                                                               <  3:174055/149‑1 (MQ=255)
                             gCCGTCCTGCAGGCAGAACTGGCCTTGCAGCTCTGCGCCTTGCTGCCACTGCGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCCGCGTGTTCGAAGTAGAAGCGTTGGCGAGTCAGTg                                                                                                               <  4:367512/149‑1 (MQ=255)
                             gCCGTCCTGCAGGCAGAACTGGCCTTGCAGCTCTGCGCCTTGCTGCCACTGCGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCCGCGTGTTCGAAGTAGAAGCGTTGGCGAGTCAGTg                                                                                                               <  4:361805/149‑1 (MQ=255)
                                gTCCTGCAGGCAGAACTGGCCTTGCAGCTCTGCGCCTTGCTGCCACTGCGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGAGTCAGTggg                                                                                                             >  6:64888/1‑148 (MQ=255)
                                   cTGCAGGCAGAACTGGCCTTGCAGCTCTGCGCCTTGCTGCCACTGCGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGGGTCAGTGGGTa                                                                                                           >  4:269261/1‑147 (MQ=255)
                                         gcagAACTGGCCTTGCAGCTCTGCGCCTTGGTGCCACTGCGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGTAGaa                                                                                                                                >  1:31303/1‑120 (MQ=255)
                                         gcagAACTGGCCTTGCAGCTCTGCGCCTTGCTGCCACTGCGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGTAGaa                                                                                                                                <  2:31303/120‑1 (MQ=255)
                                              aCTGGCCTTGCAGCTCTGCGCCTTGCTGCCACTGCGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGAGTCAGTGGGTACAGGGTCTTGaa                                                                                               >  1:109732/1‑148 (MQ=255)
                                                 gcaaggccAGTTCTGCGCCTTGCTGCCACTGCGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGAGTCAGTGGGTACAGGGTCTTGAACAAg                                                                                           >  3:219349/8‑149 (MQ=255)
                                                  gCCTTGCAGCTCTGCGCCTTGCTGCCACTGCGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGAGTCAGTGGGTACAGGGTCTTGAACAAg                                                                                           <  2:109732/148‑1 (MQ=255)
                                                  gCCTTGCAGCTCTGCGCCTTGCTGCCACTGCGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGAGTCAGTGGGTACAGGGTCTTGAACAAGc                                                                                          <  8:1121/149‑1 (MQ=255)
                                                    cTTGCAGCTCTGCGCCTTGCTGCCACTGCGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGAGTCAGTGGGTACAGGGTCTTGAACAAGCtt                                                                                        >  1:218941/1‑149 (MQ=255)
                                                      tGCAGCTCTGCGCCTTGCTGCCACTGCGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGAGTCAGTGGGTACAGGGTCTTGAACAAGCTTTc                                                                                      >  2:85126/1‑149 (MQ=255)
                                                        cAGCTCTGCGCCTTGCTGCCACTGCGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGAGTCAGTGGGTACAGGGTCTTGAACAAGCTTTcc                                                                                     <  5:242460/148‑1 (MQ=255)
                                                         aGCTCTGCGCCTTGCTGCCACTGCGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGAGTCAGTGGGTACAGGGTCTTGAACAAGCtt                                                                                        <  4:153398/144‑1 (MQ=255)
                                                         aGCTCTGCGCCTTGCTGCCACTGCGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGAGTCAGTGGGTACAGGGTCTTGAACAAGCtt                                                                                        >  3:153398/1‑144 (MQ=255)
                                                         aGCTCTGCGCCTTGCTGCCACTGCGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGAGTCAGTGGGTACAGGGTCTTGAACAAGCTTTCCtt                                                                                   <  7:272131/149‑1 (MQ=255)
                                                         aGCTCTGCGCCTTGCTGCCACTGCGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGAGTCAGTGGGTACAGGGTCTTGAACAAGCTTTCCtt                                                                                   <  5:64888/149‑1 (MQ=255)
                                                            tctGCGCCTTGCTGCCACTGCGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGAGTCAGTGGGTACAGGGTCTTGAACAAGCTTTCCTTCAg                                                                                >  6:8067/1‑149 (MQ=255)
                                                               gcgcCTTGCTGCCACTGCGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGAGTCAGTGGGTACAGGGTCTTGAACAAGCTTTcc                                                                                     <  4:335687/141‑1 (MQ=255)
                                                               gcgcCTTGCTGCCACTGCGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGAGTCAGTGGGTACAGGGTCTTGAACAAGCTTTcc                                                                                     >  3:335687/1‑141 (MQ=255)
                                                                   cTTGCTGCCACTGCGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGAGTCAGTGGGTACAGGGTCTTGAACAAGCTTTCCTTCAGCGAAAAg                                                                         >  7:100928/1‑149 (MQ=255)
                                                                       ctgcCACTGCGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGAGTCAGTGGGTACAGGGTCTTGAACAAGCTTTCCTTCa                                                                                 >  4:286957/1‑137 (MQ=255)
                                                                       ctgcCACTGCGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGAGTCAGTGGGTACAGGGTCTTGAACAAGCTTTCCTTCa                                                                                 <  3:286957/137‑1 (MQ=255)
                                                                          ccACTGCGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGAGTCAGTGGGTACAGGGTCTTGAACAAGCTTTCCTTCAGCGAAAAGGTCaa                                                                    <  7:61194/147‑1 (MQ=255)
                                                                          ccACTGCGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGAGTCAGTGGGTACAGGGTCTTGAACAAGCTTTCCTTCAGCGAAAAGGTCaa                                                                    <  8:100928/147‑1 (MQ=255)
                                                                          ccACTGCGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGAGTCAGTGGGTACAGGGTCTTGAACAAGCTTTCCTTCAGCGAAAAGGTCAAgg                                                                  <  5:8067/149‑1 (MQ=255)
                                                                           cACTGCGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGAGTCAGTGGGTACAGGGTCTTGAACAAGCTTTCCTTCAGCGAAAAg                                                                         <  2:3952/141‑1 (MQ=255)
                                                                           cACTGCGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGAGTCAGTGGGTACAGGGTCTTGAACAAGCTTTCCTTCAGCGAAAAg                                                                         >  1:3952/1‑141 (MQ=255)
                                                                                cGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGGGTCAGTGGGTACAGGGTCTTGAACAAGCTTTCCTTCAGCGAAAAGGTCAAGGTAAc                                                              <  3:269261/147‑1 (MQ=255)
                                                                                cGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGAGTCAGTGGGTACAGGGTCTTGAACAAGCTTTCCTTCAGCGAAAAGGTCAAGGTAACg                                                             <  4:219349/148‑1 (MQ=255)
                                                                                 ggTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGttg                                                                                                                                  <  7:301044/78‑3 (MQ=255)
                                                                                 ggTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGtgg                                                                                                                                  >  8:301044/1‑76 (MQ=255)
                                                                                     aCAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGAGTCAGTGGGTACAGGGTCTTGAACAAGCTTTCCTTCAGCGAAAAGGTCaa                                                                    <  8:210485/136‑1 (MQ=255)
                                                                                     aCAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGAGTCAGTGGGTACAGGGTCTTGAACAAGCTTTCCTTCAGCGAAAAGGTCaa                                                                    >  7:210485/1‑136 (MQ=255)
                                                                                                 gCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGTGCACTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGAGTCAGTGGGTACAGGGTCTTGAACAAGCTTTCCTTCAGCGAAAAGGTCAAGGTAACGGTCAGGCCGAGCTGAcgg                                           <  7:166150/149‑1 (MQ=255)
                                                                                                 gCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTg                                                                                                                          <  8:336536/70‑1 (MQ=255)
                                                                                                 gCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTg                                                                                                                          >  7:336536/1‑70 (MQ=255)
                                                                                                     gcgcAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGAGTCAGTGGGTACAGGGTCTTGAACAAGCTTTCCTTCAGCGAAAAGGTCAAGGTAACGGTCAGGCCGAGCTGACGGCGGt                                       >  1:216616/1‑149 (MQ=255)
                                                                                                       gcAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGAGTCAGTGGGTACAGGGTCTTGAACAAGCTTTCCTTCAGCGAAAAGGTCAAGGTAACg                                                             <  7:31508/125‑1 (MQ=255)
                                                                                                       gcAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGAGTCAGTGGGTACAGGGTCTTGAACAAGCTTTCCTTCAGCGAAAAGGTCAAGGTAACg                                                             >  8:31508/1‑125 (MQ=255)
                                                                                                             gcgcCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGAGTCAGTGGGTACAGGGTCTTGAACAAGCTTTCCTTCAGCGAAAAGGTCAAGGTAACGGTCAGGCCGAGCTGACGGCGGTCCAGgcgc                               >  2:238080/1‑149 (MQ=255)
                                                                                                                                ccAGTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGAGTCAGTGGGTACAGGGTCTTGAACAAGCTTTCCTTCAGCGAAAAGGTCAAGGTAACGGTCAGGCCGAGCTGACGGCGGTCCAGGCGCTCCAGCTCTGCCGGGAt              >  6:41324/1‑147 (MQ=255)
                                                                                                                                 cAGTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGAGTCAGTGGGTACAGGGTCTTGAACAAGCTTTCCTTCAGCGAAAAGGTCAAGGTAACGGTCAGGCCGAGCTGACGGCGGTCCAGGCGCTCCAGCTCTGCCGGGATAAg           <  2:216616/149‑1 (MQ=255)
                                                                                                                                 cAGTCGAGCACTTCTGCGAGTTCGAAGTAGAAGCGTTGGCGAGTCAGTGGGTACAGGGTCTTGAACAACCTTTCCTTCAGCGAAAAGCTCAAGGTAACGCTCAGGCCGAGCTGACGGCGGTCCAGGCGCTCCAGCTCTCACGGGATAAg           >  7:194686/1‑149 (MQ=255)
                                                                                                                                   gTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGAGTCAGTGGGTACAGGGTCTTGAACAAGCTTTCCTTCAGCGAAAAGGTc                                                                      >  7:120967/1‑88 (MQ=255)
                                                                                                                                   gTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGAGTCAGTGGGTACAGGGTCTTGAACAAGCTTTCCTTCAGCGAAAAGGTc                                                                      <  8:120967/88‑1 (MQ=255)
                                                                                                                                   gTCGAGCACTTCTGCGTGTTAGAAGTAGAAGCGTTGGCGAGTCAGTGGGTACAGGGTCTTGAACAAGCTTTCCTTCAGCGAAAAGGTCAAGGTAACGGTCAGGCCGAGCTGACGGCGGTCCAGGCGCTCCAGCTCTGCCGGGATAAGGa         >  5:281229/1‑149 (MQ=255)
                                                                                                                                      gAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGAGTCAGTGGGTACAGGGTCTTGAACAAGCTTTCATTCAGCGAAAAGGTCAAGGTAACGGTAAGCCCGAGCTGACGGCGGTCCAGGCGATCCAGATCTGCCGGGATAAGGAtt       >  3:131356/1‑148 (MQ=255)
                                                                                                                                        gCACTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGAGTCAGTGGGTACAGGGTCTTGAACAAGCTTTCCTTCAGCGAAAAGGTCAAGGTAACGGTCAGGCCGAGCTGACGGCGGTCCAGGCGCTCCAGCTCTGCCGGGATAAGGATTTCa    <  5:41324/149‑1 (MQ=255)
                                                                                                                                         cACTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGAGTCAGTGGGTACAGGGTCTTGAACAAGCTTTCCTTCAGCGAAAAGGTCAAGGTAACGGTCAGGCCGAGCTGACGGCGGTCCAGGCGCTCCAGCTCTGCCGGGATAAGGATTTCa    <  7:266952/148‑1 (MQ=255)
                                                                                                                                           cTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGAGTCAGTGGGTACAGGGTCTTGAACAAGCTTTCCTTCAGCGAAAAGGTCAAGGTAACGGTCAGGCCGAGCTGACGGCGGTCCAGGCGCTCCAGCTCTGCCGGGATAAGGATTTCAcc  >  4:226250/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                               
GGTCAGACGCTGACCAGGCTCAGCAGGTGGCCGTCCTGCAGGCAGAACTGGCCTTGCAGCTCTGCGCCTTGCTGCCACTGCGGTGACAGGTCGGTGAGCAGGCGCAAGCGCGCCAGCCCTTCCGCGGACCAGTCGAGCACTTCTGCGTGTTCGAAGTAGAAGCGTTGGCGAGTCAGTGGGTACAGGGTCTTGAACAAGCTTTCCTTCAGCGAAAAGGTCAAGGTAACGGTCAGGCCGAGCTGACGGCGGTCCAGGCGCTCCAGCTCTGCCGGGATAAGGATTTCACC  >  NC_002947/1358429‑1358715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑