Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,361,924 A→G 5.2% Y105C (TAT→TGT)  phoQ → sensor protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,361,9240AG 5.2% 231.4 / 2.0 76Y105C (TAT→TGT) phoQsensor protein
Reads supporting (aligned to +/- strand):  ref base A (37/35);  new base G (2/2);  total (39/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.31e-01

AGCATGGCCGGTTGCAGATGCCGACACTGCTGCCGGACGAGCGCTACAACCTGCCCTACACAGGCCTGCTCGGCTATATCTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCACCAGTCAGATGGCGAAGAGTTCTTCGTATACGACGTCGAGATAAAGCTGCTGGGCGGGCAGAGCGCCGCCTACAGCATTGTCGCTCTACAGCCCGTGAGC  >  NC_002947/1361783‑1362069
                                                                                                                                                  |                                                                                                                                                 
aGCATGGCCGGTTGCAGATGCCGACACTGCTGCCGGACGAGCGCTACAACCTGCCCTACACAGGCCTGCTCGGCTATATCTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCAGACCGGCACATCAACTATCAGCcg                                                                                                                                            >  6:250748/1‑149 (MQ=255)
     ggCCGGTTGCAGATGCCGACACTGCTGCCGGACGAGCGCTACAACCTGCCCTACACAGGCCTGCTCGGCTATATCTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTa                                                                                                                                       >  6:88330/1‑149 (MQ=255)
          gTTGCAGATGCCGACACTGCTGCCGGACGAGCGCTACAACCTGCCCTACACAGGCCTGCTCGGCTATATCTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACg                                                                                                                                  >  2:284796/1‑149 (MQ=255)
              cAGATGCCGACACTGCTGCCGGACGAGCGCTACAACCTGCCCTACACAGGCCTGCTCGGCTATATCTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGgc                                                                                                                               <  7:138532/148‑1 (MQ=255)
              cAGATGCCGACACTGCTGCCGGACGAGCGCTACAACCTGCCCTACACAGGCCTGCTCGGCTATATCTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGgc                                                                                                                               <  2:194122/148‑1 (MQ=255)
               aGATGCCGACACTGCTGCCGGACGAGCGCTACAACCTGCCCTACACAGGCCTGCTCGGCTATATCTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGgcgc                                                                                                                             <  5:173450/149‑1 (MQ=255)
                gATGCCGACACTGCTGCCGGACGAGCGCTACAACCTGCCCTACACAGGCCTGCTCGGCTATATCTTTGACCGCGAT‑GG‑‑CAAACCAGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGgcgc                                                                                                                             <  1:111418/149‑1 (MQ=255)
                    ccGACACTGCTGCCGGACGAGCGCTACAACCTGCCCTACACAGGCCTGCTCGGCTATATCTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCa                                                                                                                         <  5:280450/148‑1 (MQ=255)
                     cGACACTGCTGCCGGACGAGCGCTACAACCTGCCCTACACAGGCCTGCTCGGCTATATCTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAAc                                                                                                                       <  6:167277/149‑1 (MQ=255)
                      gACACTGCTGCCGGACGAGCGCTACAACCTGCCCTACACAGGCCTGCTCGGCTATATCTTTGACCGCGATCGGTAGAAA‑CTGGTCTGGCAAGTAGCGTGCCACCGCCGACCGGCACATCCACTATCAGCCGCGCTATGACGGGCGCgg                                                                                                                           >  1:115278/1‑148 (MQ=255)
                       acacTGCTGCCGGACGAGCGCTACAACCTGCCCTACACAGGCCTGCTCGGCTATATCTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGa                                                                                                                     <  5:88330/149‑1 (MQ=255)
                         acTGCTGCCGGACGAGCGCTACAACCTGCCCTACACAGGCCTGCTCGGCTATATCTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGTCGCGGCAACGAGt                                                                                                                   >  3:246849/1‑149 (MQ=255)
                           tgctgcCGGACGAGCGCTACAACCTGCCCTACACAGGCCTGCTCGGCTATATCTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCt                                                                                                                                        >  2:289284/1‑126 (MQ=255)
                           tgctgcCGGACGAGCGCTACAACCTGCCCTACACAGGCCTGCTCGGCTATATCTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCt                                                                                                                                        <  1:289284/126‑1 (MQ=255)
                           tgctgcCGGACGAGCGCTACAACCTGCCCTACACAGGCCTGCTCGGCTATATCTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTc                                                                                                                 >  3:370166/1‑149 (MQ=255)
                                    aCGAGCGCTACAACCTGCCCTACACAGGCCTGCTCGGCTATATCTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTc                                                                                                                 >  8:19859/1‑140 (MQ=255)
                                    aCGAGCGCTACAACCTGCCCTACACAGGCCTGCTCGGCTATATCTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTc                                                                                                                 <  7:19859/140‑1 (MQ=255)
                                      gAGCGCTACAACCTGCCCTACACAGGCCTGCTCGGCTATATCTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGt                                                                                                                   >  8:162479/1‑136 (MQ=255)
                                      gAGCGCTACAACCTGCCCTACACAGGCCTGCTCGGCTATATCTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGt                                                                                                                   <  7:162479/136‑1 (MQ=255)
                                      gAGCGCTACAACCTGCCCTACACAGGCCTGCTCGGCTATATCTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCa                                                                                                      >  3:23444/1‑149 (MQ=255)
                                       aGCGCTACAACCTGCCCTACACAGGCCTGCTCGGCTATATCTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCAc                                                                                                     <  6:26803/149‑1 (MQ=255)
                                           cTACAACCTGCCCTACACAGGCCTGCTCGGCTATATCTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCACCAGt                                                                                                 >  4:153032/1‑149 (MQ=255)
                                            tACAACCTGCCCTACACAGGCCTGCTCGGCTATATCTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCACCAGt                                                                                                 >  8:164758/1‑148 (MQ=255)
                                            tACAACCTGCCCTACACAGGCCTGCTCGGCTATATCTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCACCAGt                                                                                                 >  4:176962/1‑148 (MQ=255)
                                            tACAACCTGCCCTACACAGGCCTGCTCGGCTATATCTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCACCAGt                                                                                                 >  7:44297/1‑148 (MQ=255)
                                            tACAACCTGCCCTACACAGGCCTGCTCGGCTATATCTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAAAGAGTTCGACCGCATTCACCAGt                                                                                                 >  2:184323/1‑148 (MQ=255)
                                              cAACCTGCCCTACACAGGCCTGCTCGGCTATATCTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGAc                                                                                                                                   >  5:222452/1‑112 (MQ=255)
                                              cAACCTGCCCTACACAGGCCTGCTCGGCTATATCTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGAc                                                                                                                                   <  6:222452/112‑1 (MQ=255)
                                                 ccTGCCCTACACAGGCCTGCTCGGCTATATCTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAg                                                                                                                                               <  1:42011/97‑1 (MQ=255)
                                                 ccTGCCCTACACAGGCCTGCTCGGCTATATCTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAg                                                                                                                                               >  2:42011/1‑97 (MQ=255)
                                                   tGCCCTACACAGGCCTGCTCGGCTATATCTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCACCAGTCAGATGGc                                                                                         >  7:267192/1‑149 (MQ=255)
                                                   tGCCCTACACAGGCCTGCTCGGCTATATCTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCACCAGTCAGATGGc                                                                                         >  4:285246/1‑149 (MQ=255)
                                                     cccTACACAGGCCTGCTCGGCTATATCTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCACCAGTCAGATGGCg                                                                                        >  2:173761/1‑148 (MQ=255)
                                                     cccTACACAGGCCTGCTCGGCTATATCTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCACCAGTCAGATGGCg                                                                                        <  3:176962/148‑1 (MQ=255)
                                                        tACACAGGCCTGCTCGGCTATATCTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCACCAGTCAGATGGCGaa                                                                                      >  5:81232/1‑147 (MQ=255)
                                                         acacAGGCCTGCTCGGCTATATCTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCACCAGTCAGATGGCGAagag                                                                                   >  6:290391/1‑149 (MQ=255)
                                                           acaGGCCTGCTCGGCTATATCTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCACCAGTCAGATGGCGAAGAGtt                                                                                 <  8:44297/149‑1 (MQ=255)
                                                             aGGCCTGCTCGGCTATATCTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCACCAGTCa                                                                                               >  4:165080/1‑133 (MQ=255)
                                                             aGGCCTGCTCGGCTATATCTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCACCAGTCa                                                                                               <  3:165080/133‑1 (MQ=255)
                                                             aGGCCTGCTCGGCTATATCTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCACCAGTCAGATGGCGAAGAGttct                                                                               >  6:290530/1‑149 (MQ=255)
                                                               gCCTGCTCGGCTATATCTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCACCAGTCAGATGGCGAAGAGttcttc                                                                             <  4:370166/149‑1 (MQ=255)
                                                                 cTGCTCGGCTATATCTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCACCAGTCAGATGGCGAAGAGTTCTTCGt                                                                           <  2:115278/149‑1 (MQ=255)
                                                                        gCTATATCTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATg                                                                                                                                     >  8:158533/1‑84 (MQ=255)
                                                                        gCTATATCTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATg                                                                                                                                     <  7:158533/84‑1 (MQ=255)
                                                                        gCTATATCTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCACCAGTCAGATGGCGAAGAGTTCTTCGTATacgac                                                                    <  6:81232/149‑1 (MQ=255)
                                                                              tCTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCACCAGTCAGATGGCGAAGAGTTCTTCGTATACGACGTc                                                                 >  5:6509/1‑146 (MQ=255)
                                                                               cTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCACCAGTCAGATGGCGAAGAGTTCTTCGTATACGACGTCgaga                                                             <  3:255628/149‑1 (MQ=255)
                                                                                tttGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTGTCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCACCAGTCAGATGGCGAAGAGttcttc                                                                             >  1:69188/1‑132 (MQ=255)
                                                                                tttGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTGTCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCACCAGTCAGATGGCGAAGAGttcttc                                                                             <  2:74611/132‑1 (MQ=255)
                                                                                tttGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTGTCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCACCAGTCAGATGGCGAAGAGttcttc                                                                             <  2:69188/132‑1 (MQ=255)
                                                                                tttGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTGTCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCACCAGTCAGATGGCGAAGAGttcttc                                                                             >  1:74611/1‑132 (MQ=255)
                                                                                  tGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCACCAGTCAGATGGCGAAGAGTTCTTCGTATACGACGTCga                                                               <  5:123439/144‑1 (MQ=255)
                                                                                  tGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCACCAGTCAGATGGCGAAGAGTTCTTCGTATACGACGTCga                                                               >  6:123439/1‑144 (MQ=255)
                                                                                    aCCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCACCAGTCAGATGGCGAAGAGTTCTTCGTATACGACGTCGAGATAAAg                                                        <  4:23444/149‑1 (MQ=255)
                                                                                     ccGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCACCAGTCAGATGGCGAAGAGTTCTTCGTATACGACGTCGAGATAAAgc                                                       <  5:250748/149‑1 (MQ=255)
                                                                                          aT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCACCAGTCAGATGGCGAAGAGTTCTTCGTATACGACGTCGAGATAAAGctgctg                                                  <  1:284796/149‑1 (MQ=255)
                                                                                           t‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCACCAGTCAGATGGCGAAGAGTTCTTCGTATACGACGTCGAGATAAAGCTGCTgg                                                 <  5:19407/149‑1 (MQ=255)
                                                                                              g‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCACCAGTCAGATGGCGAAGAGTTCTTCGTATACGACGTCGAGATAAAGCTGCTgggc                                               >  1:109781/1‑149 (MQ=255)
                                                                                                    a‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCACCAGTCAGATGGCGAAGAGTTCTTCGTATACGACGTCGAGATAAAGCTGCTgggcgggc                                           <  3:285246/149‑1 (MQ=255)
                                                                                                         gTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCAt                                                                                                         <  8:98459/83‑1 (MQ=255)
                                                                                                         gTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCAt                                                                                                         >  7:98459/1‑83 (MQ=255)
                                                                                                          tCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCACCAGTCAGATGGCGAAGAGTTCTTCGTATACGACGTCGAGATAAAGCTGCTGGGCGGGCAGAgc                                      >  2:301673/1‑149 (MQ=255)
                                                                                                           cTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGCCCGCATTCACCAGTCAGATGGCGAAGAGTTCTTCGTATACGACGTCGAGATAAAGCTGCTGGGCGGGCAGAgcg                                     >  3:326217/1‑149 (MQ=255)
                                                                                                            tGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCACCAGTCAGATGGCGAAGAGTTCTTCGTATACGACGTCGAGATAAAGCTGCTGGGCGGGCAGAgcgc                                    >  8:148177/1‑149 (MQ=255)
                                                                                                              gC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCACCAGTCAGATGGCGAAGAGTTCTTCGTATACGACGTCGAGATAAAGCTGCTGGGCGGGCAGAGcgccg                                  >  5:27816/1‑149 (MQ=255)
                                                                                                              gC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCACCAGTCAGATGGCGAAGAGTTCTTCGTATACGACGTCGAGATAAAGCTGCTGGGCGGGCAGAGcgccg                                  >  2:61930/1‑149 (MQ=255)
                                                                                                                 aGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGTCGCGGCAACGAGTTCGACCGCATTCACCAGTCAGATGGCGAAGAGTTCTTCGTATACGACGTCGAGATAAAGCTGCTGGGCGGGCAGAGCgccgcc                                <  4:246849/149‑1 (MQ=255)
                                                                                                                   tCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCACCAGTCAGATGGCGAAGAGTTCTTCGTATACGACGTCGAGATAAAGCTGCTGGGCGGGCAGAGCGCCGCCTa                              >  8:221296/1‑149 (MQ=255)
                                                                                                                   tCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCACCAGTCAGATGGCGAAGAGTTCTTCGTATACGACGTCGAGATAAAGCTGCTGGGCGGGCAGAGCGCCGCCTa                              >  3:20607/1‑149 (MQ=255)
                                                                                                                     gcgTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCACCAGTCAGATGGCGAAGAGTTCTTCGTATACGACGTCGAGATAAAGCTGCTGGGCGGGCAGAGCGCCGCCTAc                             <  3:153032/148‑1 (MQ=255)
                                                                                                                          ccACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCACCAGTCAGATGGCGAAGAGTTCTTCGTATACGACGTCGAGATAAAGCTGCTGGGCGGGCAGAGCGCCGCCTACAGCAt                        <  5:290530/148‑1 (MQ=255)
                                                                                                                            aCCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCACCAGTCAGATGGCGAAGAGTTCTTCGTATACGACGTCGAGATAAAGCTGCTGGGCGGGCAGAGCGCCGCCTACAGCATTGt                     <  7:164758/149‑1 (MQ=255)
                                                                                                                              cgccgACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTACGACCGCATTCACCAGTCAGATGGCGAAGAGTTCTTCGTATACGACGTCGAGATAAAGCTGCTGGGCGGGCAGAGCGCCGCCTACAGCATTGTCg                   <  1:301673/149‑1 (MQ=255)
                                                                                                                                 cgACCGGCACATCAACTATCAGACGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCACCAGTCAGATGGCGAAGAGTTCTTCGTATACGACGTCGAGATAAAGCTGCTGGGCGGGCAGAGCGCCGCCTACAGCATTGTCGct                 >  8:116122/1‑148 (MQ=255)
                                                                                                                                           aTCAACTATCAGCCGCTCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCACCAGTCAGATGGCGAAGAGTTCTTCGTATACGACGTCGAGATAAAGCTGCTGGGCGGGCAGAGCGCCGCCTACAGCATTGTCGCTCTACAGCCCGt      <  1:184323/149‑1 (MQ=255)
                                                                                                                                               aCTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCACCAGTCAGATGGCGAAGAGTTCTTCGTATACGACGTCGAGATAAAGCTGCTGGGCGGGCAGAGCGCCGCCTACAGCATTGTCGCTCTACAGCCCGTGAGc  <  7:148177/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
AGCATGGCCGGTTGCAGATGCCGACACTGCTGCCGGACGAGCGCTACAACCTGCCCTACACAGGCCTGCTCGGCTATATCTTTGACCGCGAT‑GG‑‑CAAA‑CTGGTCTGGC‑AGTCGCGTGCCACCGCCGACCGGCACATCAACTATCAGCCGCGCTATGACGGGCGCGGCAACGAGTTCGACCGCATTCACCAGTCAGATGGCGAAGAGTTCTTCGTATACGACGTCGAGATAAAGCTGCTGGGCGGGCAGAGCGCCGCCTACAGCATTGTCGCTCTACAGCCCGTGAGC  >  NC_002947/1361783‑1362069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑