Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,390,996	G→C	6.3%	G268A (GGC→GCC)	aspS →	aspartate‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,390,996	0	G	C	6.3%	199.8 / 4.2	63	G268A (GGC→GCC)	aspS	aspartate‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (32/27);  new base C (2/2);  total (34/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.85e-01

CCGTGACGAAGACCTGCGTGCCGACCGTCAGCCGGAATTCACCCAGATCGACATCGAGACCAGTTTCCTCGA‑TGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAGGTGTTCGCCGGCCCGGCCAACGATCCG  >  NC_002947/1390850‑1391144                                                                                                                                                    |                                                                                                                                                     ccGTGACGAAGACCTGCGTGCCGACCGTCAGCCGGAATTCACCCAGATCGACATCGAGACCAGTTTCCTCGA‑TGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCgg                                                                                                                                                      <  7:164442/147‑1 (MQ=255)         aaGACCTGCGTGCCGACCGTCAGCCGGAATTCACCCAGATCGACATCGAGACCAGTTTCCTCGA‑TGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGCCGAATTccag                                                                                                                                            <  3:77185/149‑3 (MQ=255)              cTGCGTGCCGACCGTCAGCCGGAATTCACCCAGATCGACATCGAGACCAGTTTCCTCGA‑TGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACAt                                                                                                                                       >  4:265971/1‑149 (MQ=255)              cTGCGTGCCGACCGTCAGCCGGAATTCACCCAGATCGACATCGAGACCAGTTTCCTCGA‑TGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGCCGAATTccagatcga                                                                                                                                       <  3:131090/149‑8 (MQ=255)                        aCCGTCAGCCGGAATTCACCCAGATCGACATCGAGACCAGTTTCCTCGA‑TGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGaa                                                                                                                             <  7:52600/149‑1 (MQ=255)                         ccGTCAGCCGGAATTCACCCAGATCGACATCGAGACCAGTTTCCTCGA‑TGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAag                                                                                                                            <  7:41196/149‑1 (MQ=255)                            tCAGCCGGAATTCACCCAGATCGACATCGAGACCAGTTTCCTCGA‑TGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAgg                                                                                                                         <  2:161773/149‑1 (MQ=255)                               gCCGGAATTCACCCAGATCGACATCGAGACCAGTTTCCTCGA‑TGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCa                                                                                                                      >  6:364287/1‑149 (MQ=255)                                 cGGAATTCACCCAGATCGACATCGAGACCAGTTTCCTCGA‑TGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACATTCGAAGAGGCCATg                                                                                                                    >  4:210139/1‑149 (MQ=255)                                         aCCCAGATCGACATCGAGACCAGTTTCCTCGA‑TGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTa                                                                                                            >  1:94031/1‑149 (MQ=255)                                           ccAGATCGACATCGAGACCAGTTTCCTCGAGTGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTAc                                                                                                           >  1:221688/1‑149 (MQ=255)                                            cAGATCGACATCGAGACCAGTTTCCTCGA‑TGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTAc                                                                                                           >  8:247209/1‑147 (MQ=255)                                            cAGATCGACATCGAGACCAGTTTCCTCGA‑TGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTAc                                                                                                           <  7:247209/147‑1 (MQ=255)                                             aGATCGACATCGAGACCAGTTTCCTCGA‑TGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGc                                                                                                        >  5:164668/1‑149 (MQ=255)                                                  gACATCGAGACCAGTTTCCTCGA‑TGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGa                                                                                                   <  2:94031/149‑1 (MQ=255)                                                  gACATCGAGACCAGTTTCCTCGA‑TGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGa                                                                                                   <  3:265971/149‑1 (MQ=255)                                                              aGTTTCCTCGA‑TGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCt                                                                                       <  8:55507/149‑1 (MQ=255)                                                               gTTTCCTCGA‑TGAAAGCGAGATCATTGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTg                                                                                      >  6:258376/1‑149 (MQ=255)                                                                  tCCTCGA‑TGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCg                                                                                    >  1:314727/1‑148 (MQ=255)                                                                    cTCGT‑TGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTAt                                                                                 >  2:121128/1‑149 (MQ=255)                                                                     tCGA‑TGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTAtt                                                                                >  3:90261/1‑149 (MQ=255)                                                                       gA‑TGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTcc                                                                              >  1:152746/1‑149 (MQ=255)                                                                        a‑TGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCg                                                                             >  7:85118/1‑149 (MQ=255)                                                                          tGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGc                                                                            <  5:364287/149‑1 (MQ=255)                                                                           gAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCt                                                                           >  6:341649/1‑149 (MQ=255)                                                                             aaGCGAGATCATTGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTg                                                                          <  5:258376/148‑1 (MQ=255)                                                                              aGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTgg                                                                         >  3:162302/1‑148 (MQ=255)                                                                                         gggCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACg                                                             >  2:200384/1‑149 (MQ=255)                                                                                                aCCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAGATCCCGCACATGAACTTCGAAGAGGCCATGCGACGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGa                                                      >  1:102707/1‑149 (MQ=255)                                                                                                aCCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGa                                                      <  1:200384/149‑1 (MQ=255)                                                                                                  cGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGAc                                                     >  3:118125/1‑148 (MQ=255)                                                                                                    aaaGCATGATCCGCAAGCTGTTCATGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGCCAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCgcgcag                                                  >  6:333926/1‑144 (MQ=255)                                                                                                         aTGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGaa                                             <  3:210139/149‑1 (MQ=255)                                                                                                          tGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAg                                            >  8:285964/1‑149 (MQ=255)                                                                                                             tcagCAAGCTGTTCAAGTAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGAt                                         <  2:102707/146‑1 (MQ=255)                                                                                                                 cAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTc                                      >  8:124590/1‑148 (MQ=255)                                                                                                                  aaGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGa                                    <  5:341649/149‑1 (MQ=255)                                                                                                                        ttCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGc                                                         >  4:229151/1‑122 (MQ=255)                                                                                                                        ttCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGc                                                         <  3:229151/122‑1 (MQ=255)                                                                                                                        ttCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCaa                              <  6:164668/149‑1 (MQ=255)                                                                                                                          cAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGAc                                                     >  8:159695/1‑124 (MQ=255)                                                                                                                          cAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGAc                                                     <  7:237798/124‑1 (MQ=255)                                                                                                                          cAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGAc                                                     <  7:159695/124‑1 (MQ=255)                                                                                                                          cAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGAc                                                     >  8:237798/1‑124 (MQ=255)                                                                                                                          cAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAg                             <  4:90261/148‑1 (MQ=255)                                                                                                                          cAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAg                             <  7:285964/148‑1 (MQ=255)                                                                                                                          cAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAg                             <  2:221688/148‑1 (MQ=255)                                                                                                                            aGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAg                             >  1:210413/1‑146 (MQ=255)                                                                                                                            aGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAg                             <  2:260364/146‑1 (MQ=255)                                                                                                                            aGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAg                             <  2:210413/146‑1 (MQ=255)                                                                                                                            aGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAg                             >  1:260364/1‑146 (MQ=255)                                                                                                                                   gCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAg                                            <  4:289215/124‑1 (MQ=255)                                                                                                                                   gCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAg                                            >  3:289215/1‑124 (MQ=255)                                                                                                                                    cTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAGGTGTTCGccgg                  <  7:124590/149‑1 (MQ=255)                                                                                                                                       gATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAGGTGTTCGccggccc               >  2:289061/1‑149 (MQ=255)                                                                                                                                       gATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAGGTGTTCGccggcc                <  5:244741/148‑1 (MQ=255)                                                                                                                                          cgggAATTCGCCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAGGTGTTCGccggcccggc            >  3:347631/3‑149 (MQ=255)                                                                                                                                          cTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAGGTGTTCGccggcccggc            >  7:32290/1‑149 (MQ=255)                                                                                                                                           gggAATTCGCCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAGGTGTTCGccggcccggc            >  5:367061/2‑148 (MQ=255)                                                                                                                                            ggAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAGGTGTTCGCcggcccggcc           >  7:208537/1‑148 (MQ=255)                                                                                                                                             gAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAGGTGTTCGccggcccg              <  8:85118/144‑1 (MQ=255)                                                                                                                                             gAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAGGTGTTCGCCGGCCCGGCCaa         >  2:49974/1‑149 (MQ=255)                                                                                                                                                  cGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAg                             >  8:182059/1‑124 (MQ=255)                                                                                                                                                  cGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAg                             <  7:182059/124‑1 (MQ=255)                                                                                                                                                    gCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAGGTGTTCGCCGGCCCGGCCAACGATCCg  <  1:121128/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                     CCGTGACGAAGACCTGCGTGCCGACCGTCAGCCGGAATTCACCCAGATCGACATCGAGACCAGTTTCCTCGA‑TGAAAGCGAGATCATGGGCCTGACCGAAAGCATGATCCGCAAGCTGTTCAAGGAAGTGCTCGATCTGGAATTCGGCGAATTCCCGCACATGACCTTCGAAGAGGCCATGCGCCGCTACGGCTCCGACAAGCCAGACCTGCGTATTCCGCTGGAACTGGTCGACGTTGCCGACCAGCTGAAGGATGTCGACTTCAAGGTGTTCGCCGGCCCGGCCAACGATCCG  >  NC_002947/1390850‑1391144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑