Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,412,629	G→A	6.1%	G105S (GGC→AGC)	PP_1234 →	permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,412,629	0	G	A	6.1%	196.9 / 2.5	65	G105S (GGC→AGC)	PP_1234	permease
Reads supporting (aligned to +/- strand):  ref base G (30/31);  new base A (2/2);  total (32/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

TCAACGCCCTGGAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGTGTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGTATTGCACGCCATTGAGTCGGTGCGCGGTGAAATTGGCAAGTTTGGTCAATGGGCGCTGACCTTCTCGC  >  NC_002947/1412483‑1412773                                                                                                                                                    |                                                                                                                                                 tCAACGCCCTGGAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGTGTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCggg                                                                                                                                                 >  1:270402/1‑148 (MQ=255) tCAACGCCCTGGAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGTGTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCggg                                                                                                                                                 >  6:98343/1‑148 (MQ=255) tCAACGCCCTGGAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGTGTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGc                                                                                                                                                <  5:79221/149‑1 (MQ=255)      gCCCTGGAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGTGTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCt                                                                                                                                           >  6:243907/1‑149 (MQ=255)        ccTGGAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGTGTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTc                                                                                                                                          <  5:106662/148‑1 (MQ=255)        ccTGGAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGTGTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTc                                                                                                                                          >  6:106662/1‑148 (MQ=255)        ccTGGAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGTGTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCg                                                                                                                                         <  6:195571/149‑1 (MQ=255)          tGGAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGTGTTCGCCTTGTTAATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCgg                                                                                                                                        >  1:257245/1‑148 (MQ=255)            gAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGTGTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGc                                                                                                                                       >  2:190345/1‑147 (MQ=255)              gcgcgtgcgcgtACCTACACGGCTGGCGGTGATGCTGGTGTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGCATGCCCGGCA‑GCTCgttgaac                                                                                                                                   <  4:369885/149‑7 (MQ=255)                gcgtgcgcgtACCTACACGGCTGGCGGTGATGCTGGTGTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTgg                                                                                                                                 <  5:243907/149‑1 (MQ=255)                  gtgcgcgtACCTACACGGCTGGCGGTGATGCTGGTGTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCAGGCATGCTCGGCAAGTgg                                                                                                                                 >  1:51803/1‑147 (MQ=255)                         tACCTACACGGCTGGCGGTGATGCTGGTGTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCgttg                                                                                                                        <  5:98343/149‑1 (MQ=255)                          aCCTACACGGCTGGCGGTGATGCTGGTGTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCgttgt                                                                                                                       >  3:323898/1‑149 (MQ=255)                           ccTACACGGCTGGAGGTGATGCTGGTGTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCgttgt                                                                                                                       <  1:137411/148‑1 (MQ=255)                            cTACACGGCTGGCGGTGATGCTGGTGTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGttgttg                                                                                                                     <  8:25549/149‑1 (MQ=255)                               cacGGCTGGCGGTGATGCTGGTGTTCGCCTTGTTCATGGGCGCACTGGCGGTATTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTtgctg                                                                                                                  >  2:35641/1‑149 (MQ=255)                                 cGGCTGGCGGTGATGCTGGTGTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTtgctgct                                                                                                                <  1:190345/149‑1 (MQ=255)                                  ggCTGGCGGTGATGCTGGTGTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTtgctgctg                                                                                                               <  7:215354/149‑1 (MQ=255)                                       gCGGTGATGCTGGTGTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGcc                                                                                                          <  4:191987/149‑1 (MQ=255)                                                        cGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAg                                                                                                      >  3:56048/1‑136 (MQ=255)                                                        cGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAg                                                                                                      <  4:56048/136‑1 (MQ=255)                                                                 cATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCg                                                                                 <  2:257245/148‑1 (MQ=255)                                                                   tGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCAGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGAc                                                                              <  2:51803/149‑1 (MQ=255)                                                                              gCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGTAtt                                                                   >  7:111046/1‑149 (MQ=255)                                                                                  tgtTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGTATTGCAc                                                               <  4:323898/149‑1 (MQ=255)                                                                                      cATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGTATTGCACGCCa                                                           >  4:286015/1‑149 (MQ=255)                                                                                      cATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGTATTGCACGCCa                                                           >  4:315119/1‑149 (MQ=255)                                                                                       aTGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGTATTGCACGCCAt                                                          >  7:260380/1‑149 (MQ=255)                                                                                        tgctggtgctggTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGTATTGCACGCCAtt                                                         <  1:35641/149‑1 (MQ=255)                                                                                             gtgctggtACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGTATTGCACGCCATTGAGt                                                     <  8:111046/148‑1 (MQ=255)                                                                                                 tggtACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGTTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGTATTGCACGCCATTGAGTCGGTg                                                >  7:53868/1‑149 (MQ=255)                                                                                                 tggtACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGTATTGCACGCCATTGAGTCGGTg                                                >  6:320094/1‑149 (MQ=255)                                                                                                   gtACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTa                                                                                                 >  5:337743/1‑98 (MQ=255)                                                                                                   gtACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTa                                                                                                 <  6:337743/98‑1 (MQ=255)                                                                                                   gtACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACCAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGAAGGTATTGCACGCCATTGAGTCGCTgcg                                              >  2:351157/1‑149 (MQ=255)                                                                                                    tACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGTATTGCACGCCATTGAGTCGGTgcgc                                             <  3:315119/149‑1 (MQ=255)                                                                                                      ccGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑AGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGTATTGCACGCCATTGAGTCGGTGcgcg                                            <  5:320094/148‑1 (MQ=255)                                                                                                       cGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGa                                                                               <  8:147500/112‑1 (MQ=255)                                                                                                       cGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGa                                                                               >  7:147500/1‑112 (MQ=255)                                                                                                       cGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGTATTg                                                                  >  5:292881/1‑125 (MQ=255)                                                                                                       cGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGTATTg                                                                  <  6:292881/125‑1 (MQ=255)                                                                                                       cGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGAATTGCACGCCAtt                                                         >  5:315969/1‑134 (MQ=255)                                                                                                       cGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGAATTGCACGCCAtt                                                         <  6:315969/134‑1 (MQ=255)                                                                                                             tgtgGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTg                                                                                       >  5:305639/1‑98 (MQ=255)                                                                                                             tgtgGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTg                                                                                       <  6:305639/98‑1 (MQ=255)                                                                                                                   aCCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGTATTGCACGCCATTGAGTCGGTGCGCGGTGAAATTGGCAAg                              >  4:100647/1‑149 (MQ=255)                                                                                                                           aTCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGAc                                                                              <  4:76492/93‑1 (MQ=255)                                                                                                                           aTCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGAc                                                                              >  3:76492/1‑93 (MQ=255)                                                                                                                             cACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGTATTGCACGCCATTGAGTCGGTGCGCGGTGAAATTGGCAAGTTTGGTCAAt                    >  1:60979/1‑149 (MQ=255)                                                                                                                             cACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGTATTGCACGCCATTGAGTCGGTGCGCGGTGAAATTGGCAAGTTTGGTCAAt                    >  3:259485/1‑149 (MQ=255)                                                                                                                               cGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGTATTGCACGCCATTGAGTCGGTGCGCGGTGAAATTGGCAAGTTTGGTCAATgg                  <  2:270402/149‑1 (MQ=255)                                                                                                                                gCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGTATTGCACGCCAtt                                                         >  5:148789/1‑109 (MQ=255)                                                                                                                                gCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGTATTGCACGCCAtt                                                         <  6:148789/109‑1 (MQ=255)                                                                                                                                  tGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGTATTGCACGCCATTGAGTCGGTGCGCGGTGAAATTGGCAAGTTTGGTCAATGGgcg               >  1:191010/1‑149 (MQ=255)                                                                                                                                   gTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGTATTGCACGCCATTGAGTCGGTGCGCGGTGAAATTGGCAAGTTTGGTCAATGGgcgc              >  5:135450/1‑149 (MQ=255)                                                                                                                                      cAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGTATTGCACGCCATTGAGTCGGTGCGCGGTGAAATTGGCAAGTTTGGTCAATGGGCGCTg            <  2:60979/148‑1 (MQ=255)                                                                                                                                       aaCGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGTATTGCACGCCATTGAGTCGGTGCGCGGTGAAATTGGCa                                >  1:333247/1‑127 (MQ=255)                                                                                                                                       aaCGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGTATTGCACGCCATTGAGTCGGTGCGCGGTGAAATTGGCa                                <  2:333247/127‑1 (MQ=255)                                                                                                                                         cGAGC‑TGCCGGTCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGTATTGCACGCCATTGAGTCGGTGCGCGGTGAAATTGGCAAGTTTGGTCAATGGgcgc              <  1:29616/143‑1 (MQ=255)                                                                                                                                         cGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGTATTGCACGCCATTGAGTCGGTGCGCGGTGAAATTGGCAAGTTTGGTCAATGGgcgc              >  2:29616/1‑143 (MQ=255)                                                                                                                                             cttGCCGAGCATGCCCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGTATTGCACGCCATTGAGTCGGTGCGCGGTGAAATTGGCAAGTTTGGTCAATGGGCGCTg            >  5:128268/3‑142 (MQ=255)                                                                                                                                             cttGCCGAGCATGCCCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGTATTCCACGCCATTGAGTCGGTGCGCGGTGAAATTGGCAAGTTTGGTCAATGGGCGCTg            <  6:128268/140‑1 (MQ=255)                                                                                                                                             c‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGTATTGCACGCCATTGAGTCGGTGCGCGGTGAAATTGGCAAGTTTGGTCAATGGGCGCTGACCTtctc    <  4:259485/149‑1 (MQ=255)                                                                                                                                              ttGCCGAGCATGCCCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGTATTGCACGCCATTGAGTCGGTGCGCGGTGAAATTGGCAAGTTTGGTCAATGGgc                <  4:176548/136‑1 (MQ=255)                                                                                                                                              ttGCCGAGCATGCCCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGTATTGCACGCCATTGAGTCGGTGCGCGGTGAAATTGGCAAGTTTGGTCAATGGgc                >  3:176548/2‑137 (MQ=255)                                                                                                                                                gCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGTATTGCACGCCATTGAGTCGGTGCGCGGTGAAATTGGCAAGTTTGGTCAATGGGCGCTGACCTTCTCGc  >  7:238022/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                 TCAACGCCCTGGAGCGCGTGCGCGTACCTACACGGCTGGCGGTGATGCTGGTGTTCGCCTTGTTCATGGGCGCACTGGCGGTGTTCATGCTGGTGCTGGTACCGCTGCTGTGGCACCAGCTGATCACGCTGTTCAACGAGC‑TGCCGGGCATGCTCGGCAAGTGGCAGTCGTTGTTGCTGCTGCTGCCGGAGCGCTATCCGCATCTGGTGTCGGACGAGCAGGTATTGCACGCCATTGAGTCGGTGCGCGGTGAAATTGGCAAGTTTGGTCAATGGGCGCTGACCTTCTCGC  >  NC_002947/1412483‑1412773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑