Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,495,650	G→A	7.2%	A27T (GCC→ACC)	mdeA →	methionine gamma‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,495,650	0	G	A	7.2%	207.8 / 3.1	69	A27T (GCC→ACC)	mdeA	methionine gamma‑lyase
Reads supporting (aligned to +/- strand):  ref base G (32/32);  new base A (3/2);  total (35/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCTATTTTGGAAAAAATCGGGCAGCTCGGTGGCATAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTGGCCTTGCTGGAGCAGCGCATGGCT  >  NC_002947/1495504‑1495796                                                                                                                                                    |                                                                                                                                                    ggCTATTTTGGAAAAAATCGGGCAGCTCGGTGGCATAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGcc                                                                                                                                                   <  3:225440/149‑1 (MQ=255)     aTTTTGGAAAAAATCGGGCAGCTCGGTGGCATAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTgg                                                                                                                                               >  2:234698/1‑149 (MQ=255)      ttttTGTAAAAATCGGGCAGCTCGGTGGCTTAAGCTTATAGCAAACCACAAGTGGCTGTTGCCATGCGCGACTCCCACCACAACACAGGTTT‑TTCCACGGGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGt                                                                                                                                              <  5:70801/149‑1 (MQ=255)        ttGGAAAAAATCGGGCAGCTCGGTGGCATAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTTCTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGt                                                                                                                                              <  1:101942/148‑1 (MQ=255)         tGGAAAAAATCGGGCAGCTCGGTGGCATAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCACACCACAACACCGGTTT‑TTCCACGCGGGACATTCAACATGTCTATGACCCGCTTTACCATGGTGGCGACCTGGTGc                                                                                                                                            >  6:340338/1‑148 (MQ=255)          ggAAAAAATCGGGCAGCTCGGTGGCATAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCa                                                                                                                                          >  4:234406/1‑149 (MQ=255)            aaaaaaTCGGGCAGCTCGGTGGCATAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCACCAGGGCGCCAc                                                                                                                                         >  1:364102/1‑148 (MQ=255)                aaTCGGGCAGCTCGGTGGCATAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGgtg                                                                                                                                    >  3:159694/1‑149 (MQ=255)                aaTCGGGCAGCTCGGTGGCATAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCACCAGGGCGCCAccatgg                                                                                                                                    <  2:364102/149‑5 (MQ=255)                          cTCGGTGGCATAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACg‑g                                                                                                                         <  3:234406/149‑1 (MQ=255)                              gTGGCATAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATTGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTAc                                                                                                                     <  8:303994/149‑1 (MQ=255)                                         ttATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTa                                                                                                                      >  1:104165/1‑137 (MQ=255)                                         ttATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTa                                                                                                                      <  2:104165/137‑1 (MQ=255)                                           ataGCAAACCACAAGAGGCTGTTGCCATGCCCGACTCCCACCACAACACCGGTAT‑TTCCACGCGGGCCATTCATCATGGCTATCACCCGCTTTCCCATCGTGGCACCAGGGCGCCAccaa                                                                                                                                      >  5:74683/1‑118 (MQ=255)                                             aGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCAc                                                                                                      >  3:338976/1‑149 (MQ=255)                                               cAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCACCAGGGCGCCAccat                                                                                                                                      >  2:275006/1‑114 (MQ=255)                                               cAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCACCAGGGCGCCAccat                                                                                                                                      <  1:275006/116‑3 (MQ=255)                                                aaaCCAAAAGAGGCTGTTGCCATGCGCTACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGTCTATGACCCGCTTTCACATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGt                                                                                                   <  5:340338/149‑1 (MQ=255)                                                 aaCCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCAc                                                                                                                                         >  5:375383/1‑111 (MQ=255)                                                 aaCCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCAc                                                                                                                                         <  6:375383/111‑1 (MQ=255)                                                 aaCCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTc                                                                                                  <  7:231655/149‑1 (MQ=255)                                                    cacaAGAGGCTGTTGCCATGCGCGACTCCCACCACACCACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAg                                                                                               >  5:69188/1‑149 (MQ=255)                                                      caAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTa                                                                                             >  3:78383/1‑149 (MQ=255)                                                      caAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTa                                                                                             >  2:278373/1‑149 (MQ=255)                                                        agagGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACg                                                                                           <  4:159694/149‑1 (MQ=255)                                                         gagGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACgg                                                                                          >  1:203372/1‑149 (MQ=255)                                                         gagGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACgg                                                                                          >  8:284785/1‑149 (MQ=255)                                                          agGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGt                                                                                         <  1:234698/149‑1 (MQ=255)                                                             cTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGtgct                                                                                      >  6:243094/1‑149 (MQ=255)                                                                         gcgACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGcc                                                                          <  2:203372/149‑1 (MQ=255)                                                                             cTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGCGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCggg                                                                       <  1:278373/149‑1 (MQ=255)                                                                                ccaccaCAACACCGGTTT‑TTCCACGCGGTCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGACACCGGTGTACCAGACG‑GCTACCTATTCCTTCACCACTGTCGAGTACGGTGCTGCCTGCTTCGCCggg                                                                       <  7:161935/145‑1 (MQ=255)                                                                                ccaccaCAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATg                                                                                                                <  2:93422/104‑1 (MQ=255)                                                                                ccaccaCAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATg                                                                                                                >  1:93422/1‑104 (MQ=255)                                                                                ccaccaCAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGggag                                                                    >  5:245616/1‑148 (MQ=255)                                                                                ccaccaCAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGggag                                                                    >  6:335135/1‑148 (MQ=255)                                                                                ccaccaCAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGgga                                                                     <  7:284785/147‑1 (MQ=255)                                                                                ccaccaCAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACACGCTTTCACATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCAAATGTCGAGTACGGTGCTGCATTCATCGACGGggata                                                                   >  8:161935/1‑147 (MQ=255)                                                                                 caccacAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGggag                                                                    >  5:70206/1‑147 (MQ=255)                                                                                    cacaACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGcc                                                               >  2:239846/1‑149 (MQ=255)                                                                                     acaacaCCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCt                                                              <  1:239846/149‑1 (MQ=255)                                                                                         cacCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGc                                                          <  4:78383/149‑1 (MQ=255)                                                                                          acCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCa                                                         >  7:103308/1‑149 (MQ=255)                                                                                            cGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGt                                                                                         >  1:47590/1‑115 (MQ=255)                                                                                            cGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGt                                                                                         <  2:47590/115‑1 (MQ=255)                                                                                              gTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGggagga                                                                  >  5:144045/1‑136 (MQ=255)                                                                                              gTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGggagga                                                                  <  6:144045/136‑1 (MQ=255)                                                                                                   ttCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTAc                                                  <  8:1250/148‑1 (MQ=255)                                                                                                   ttCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTAc                                                  <  1:286613/148‑1 (MQ=255)                                                                                                    tCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACa                                                 <  6:69188/148‑1 (MQ=255)                                                                                                      cACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGcc                                              >  6:176014/1‑149 (MQ=255)                                                                                                      cACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCACCGGggagga                                                                  <  5:184619/129‑1 (MQ=255)                                                                                                      cACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCACCGGggagga                                                                  >  6:184619/1‑129 (MQ=255)                                                                                                       aCGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCg                                             >  7:161766/1‑149 (MQ=255)                                                                                                               cATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTcc                                      >  3:211256/1‑148 (MQ=255)                                                                                                               cATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTcc                                      >  2:43542/1‑148 (MQ=255)                                                                                                                 ttCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAAc                                   <  1:80765/149‑1 (MQ=255)                                                                                                                    atcatGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAAccc                                 >  1:118531/1‑148 (MQ=255)                                                                                                                         ggCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGtgctgc                                                                                    <  3:35987/92‑1 (MQ=255)                                                                                                                         ggCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGtgctgc                                                                                    >  4:35987/1‑92 (MQ=255)                                                                                                                          gCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTg                          <  4:211256/149‑1 (MQ=255)                                                                                                                                  ccGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTGGCCTTGCt                  >  7:271941/1‑149 (MQ=255)                                                                                                                                    gCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTGGCCTTGCTgg                <  5:243094/149‑1 (MQ=255)                                                                                                                                          ccATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTGGCCTTGCTGGagcagc          <  8:103308/149‑1 (MQ=255)                                                                                                                                          ccATGGTGGCGCCCTGGTGACACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTGGCCTTGCTGGagcagc          >  1:141510/1‑149 (MQ=255)                                                                                                                                           cATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTGGCCTTGCTGGAGCAgcg         >  5:286993/1‑149 (MQ=255)                                                                                                                                               gtggCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTGGCCTTGCTGGAGCAGCGCAt      <  5:335135/148‑1 (MQ=255)                                                                                                                                               gtggCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTGGCCTTGCTGGAGCAGCGCAt      <  8:161766/148‑1 (MQ=255)                                                                                                                                                tggCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTGGCCTTGCTGGAGCAGCGCATgg    <  5:176014/149‑1 (MQ=255)                                                                                                                                                  gcgcCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTGGCCTTGCTGGAGCAGCGCATGGCt  >  2:207987/1‑149 (MQ=255)                                                                                                                                                    gcCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTGGCCTTGCt                  <  7:39886/131‑1 (MQ=255)                                                                                                                                                    gcCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTGGCCTTGCt                  >  8:39886/1‑131 (MQ=255)                                                                                                                                                    |                                                                                                                                                    GGCTATTTTGGAAAAAATCGGGCAGCTCGGTGGCATAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTGGCCTTGCTGGAGCAGCGCATGGCT  >  NC_002947/1495504‑1495796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑