Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,495,654	T→G	6.9%	L28R (CTG→CGG) ‡	mdeA →	methionine gamma‑lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,495,654	0	T	G	6.9%	226.5 / 6.2	72	L28R (CTG→CGG) ‡	mdeA	methionine gamma‑lyase
Reads supporting (aligned to +/- strand):  ref base T (34/33);  new base G (3/2);  total (37/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

ATTTTGGAAAAAATCGGGCAGCTCGGTGGCATAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTGGCCTTGCTGGAGCAGCGCATGGCTTCGC  >  NC_002947/1495508‑1495800                                                                                                                                                    |                                                                                                                                                    aTTTTGGAAAAAATCGGGCAGCTCGGTGGCATAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTgg                                                                                                                                                   >  2:234698/1‑149 (MQ=255)  ttttTGTAAAAATCGGGCAGCTCGGTGGCTTAAGCTTATAGCAAACCACAAGTGGCTGTTGCCATGCGCGACTCCCACCACAACACAGGTTT‑TTCCACGGGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGt                                                                                                                                                  <  5:70801/149‑1 (MQ=255)    ttGGAAAAAATCGGGCAGCTCGGTGGCATAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTTCTTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGt                                                                                                                                                  <  1:101942/148‑1 (MQ=255)     tGGAAAAAATCGGGCAGCTCGGTGGCATAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCACACCACAACACCGGTTT‑TTCCACGCGGGACATTCAACATGTCTATGACCCGCTTTACCATGGTGGCGACCTGGTGc                                                                                                                                                >  6:340338/1‑148 (MQ=255)      ggAAAAAATCGGGCAGCTCGGTGGCATAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCa                                                                                                                                              >  4:234406/1‑149 (MQ=255)        aaaaaaTCGGGCAGCTCGGTGGCATAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCACCAGGGCGCCAc                                                                                                                                             >  1:364102/1‑148 (MQ=255)            aaTCGGGCAGCTCGGTGGCATAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGgtg                                                                                                                                        >  3:159694/1‑149 (MQ=255)            aaTCGGGCAGCTCGGTGGCATAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCACCAGGGCGCCAccatgg                                                                                                                                        <  2:364102/149‑5 (MQ=255)                      cTCGGTGGCATAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACg‑g                                                                                                                             <  3:234406/149‑1 (MQ=255)                          gTGGCATAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATTGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTAc                                                                                                                         <  8:303994/149‑1 (MQ=255)                                     ttATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTa                                                                                                                          >  1:104165/1‑137 (MQ=255)                                     ttATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTa                                                                                                                          <  2:104165/137‑1 (MQ=255)                                       ataGCAAACCACAAGAGGCTGTTGCCATGCCCGACTCCCACCACAACACCGGTAT‑TTCCACGCGGGCCATTCATCATGGCTATCACCCGCTTTCCCATCGTGGCACCAGGGCGCCAccaa                                                                                                                                          >  5:74683/1‑118 (MQ=255)                                         aGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCAc                                                                                                          >  3:338976/1‑149 (MQ=255)                                           cAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCACCAGGGCGCCAccat                                                                                                                                          <  1:275006/116‑3 (MQ=255)                                           cAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCACCAGGGCGCCAccat                                                                                                                                          >  2:275006/1‑114 (MQ=255)                                            aaaCCAAAAGAGGCTGTTGCCATGCGCTACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGTCTATGACCCGCTTTCACATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGt                                                                                                       <  5:340338/149‑1 (MQ=255)                                             aaCCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCAc                                                                                                                                             >  5:375383/1‑111 (MQ=255)                                             aaCCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCAc                                                                                                                                             <  6:375383/111‑1 (MQ=255)                                             aaCCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTc                                                                                                      <  7:231655/149‑1 (MQ=255)                                                cacaAGAGGCTGTTGCCATGCGCGACTCCCACCACACCACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAg                                                                                                   >  5:69188/1‑149 (MQ=255)                                                  caAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTa                                                                                                 >  3:78383/1‑149 (MQ=255)                                                  caAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTa                                                                                                 >  2:278373/1‑149 (MQ=255)                                                    agagGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACg                                                                                               <  4:159694/149‑1 (MQ=255)                                                     gagGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACgg                                                                                              >  8:284785/1‑149 (MQ=255)                                                     gagGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACgg                                                                                              >  1:203372/1‑149 (MQ=255)                                                      agGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGt                                                                                             <  1:234698/149‑1 (MQ=255)                                                         cTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGtgct                                                                                          >  6:243094/1‑149 (MQ=255)                                                                     gcgACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGcc                                                                              <  2:203372/149‑1 (MQ=255)                                                                         cTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACGCGCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCggg                                                                           <  1:278373/149‑1 (MQ=255)                                                                            ccaccaCAACACCGGTTT‑TTCCACGCGGTCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGACACCGGTGTACCAGACG‑GCTACCTATTCCTTCACCACTGTCGAGTACGGTGCTGCCTGCTTCGCCggg                                                                           <  7:161935/145‑1 (MQ=255)                                                                            ccaccaCAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATg                                                                                                                    <  2:93422/104‑1 (MQ=255)                                                                            ccaccaCAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATg                                                                                                                    >  1:93422/1‑104 (MQ=255)                                                                            ccaccaCAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGggag                                                                        >  5:245616/1‑148 (MQ=255)                                                                            ccaccaCAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGggag                                                                        >  6:335135/1‑148 (MQ=255)                                                                            ccaccaCAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGgga                                                                         <  7:284785/147‑1 (MQ=255)                                                                            ccaccaCAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACACGCTTTCACATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCAAATGTCGAGTACGGTGCTGCATTCATCGACGGggata                                                                       >  8:161935/1‑147 (MQ=255)                                                                             caccacAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGggag                                                                        >  5:70206/1‑147 (MQ=255)                                                                                cacaACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGcc                                                                   >  2:239846/1‑149 (MQ=255)                                                                                 acaacaCCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCt                                                                  <  1:239846/149‑1 (MQ=255)                                                                                     cacCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGc                                                              <  4:78383/149‑1 (MQ=255)                                                                                      acCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCa                                                             >  7:103308/1‑149 (MQ=255)                                                                                        cGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGt                                                                                             >  1:47590/1‑115 (MQ=255)                                                                                        cGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGt                                                                                             <  2:47590/115‑1 (MQ=255)                                                                                          gTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGggagga                                                                      >  5:144045/1‑136 (MQ=255)                                                                                          gTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGggagga                                                                      <  6:144045/136‑1 (MQ=255)                                                                                               ttCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTAc                                                      <  8:1250/148‑1 (MQ=255)                                                                                               ttCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTAc                                                      <  1:286613/148‑1 (MQ=255)                                                                                                tCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACa                                                     <  6:69188/148‑1 (MQ=255)                                                                                                  cACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGcc                                                  >  6:176014/1‑149 (MQ=255)                                                                                                  cACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCACCGGggagga                                                                      >  6:184619/1‑129 (MQ=255)                                                                                                  cACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCACCGGggagga                                                                      <  5:184619/129‑1 (MQ=255)                                                                                                   aCGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCg                                                 >  7:161766/1‑149 (MQ=255)                                                                                                           cATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTcc                                          >  3:211256/1‑148 (MQ=255)                                                                                                           cATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTcc                                          >  2:43542/1‑148 (MQ=255)                                                                                                             ttCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAAc                                       <  1:80765/149‑1 (MQ=255)                                                                                                                atcatGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAAccc                                     >  1:118531/1‑148 (MQ=255)                                                                                                                     ggCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGtgctgc                                                                                        <  3:35987/92‑1 (MQ=255)                                                                                                                     ggCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGtgctgc                                                                                        >  4:35987/1‑92 (MQ=255)                                                                                                                      gCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTg                              <  4:211256/149‑1 (MQ=255)                                                                                                                              ccGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTGGCCTTGCt                      >  7:271941/1‑149 (MQ=255)                                                                                                                                gCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTGGCCTTGCTgg                    <  5:243094/149‑1 (MQ=255)                                                                                                                                      ccATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTGGCCTTGCTGGagcagc              <  8:103308/149‑1 (MQ=255)                                                                                                                                      ccATGGTGGCGCCCTGGTGACACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTGGCCTTGCTGGagcagc              >  1:141510/1‑149 (MQ=255)                                                                                                                                       cATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTGGCCTTGCTGGAGCAgcg             >  5:286993/1‑149 (MQ=255)                                                                                                                                           gtggCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTGGCCTTGCTGGAGCAGCGCAt          <  8:161766/148‑1 (MQ=255)                                                                                                                                           gtggCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTGGCCTTGCTGGAGCAGCGCAt          <  5:335135/148‑1 (MQ=255)                                                                                                                                            tggCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTGGCCTTGCTGGAGCAGCGCATgg        <  5:176014/149‑1 (MQ=255)                                                                                                                                              gcgcCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTGGCCTTGCTGGAGCAGCGCATGGCt      >  2:207987/1‑149 (MQ=255)                                                                                                                                                gcCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTGGCCTTGCt                      <  7:39886/131‑1 (MQ=255)                                                                                                                                                gcCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTGGCCTTGCt                      >  8:39886/1‑131 (MQ=255)                                                                                                                                                  ccTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGGGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTGGCCTTGCTGGAGCAGCGCATGGCTTCGc  <  4:338976/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    ATTTTGGAAAAAATCGGGCAGCTCGGTGGCATAAGCTTATAGCAAACCACAAGAGGCTGTTGCCATGCGCGACTCCCACCACAACACCGGTTT‑TTCCACGCGGGCCATTCATCATGGCTATGACCCGCTTTCCCATGGTGGCGCCCTGGTGCCACCGGTGTACCAGACG‑GCTACCTATGCCTTCCCCACTGTCGAGTACGGTGCTGCCTGCTTCGCCGGGGAGGAGCCTGGGCATTTCTACAGCCGCATTTCCAACCCCACGCTGGCCTTGCTGGAGCAGCGCATGGCTTCGC  >  NC_002947/1495508‑1495800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑