Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 170,277 A→T 8.7% T227S (ACC→TCC)  PP_0159 → family III CoA‑transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947170,2770AT 8.7% 124.6 / 3.8 46T227S (ACC→TCC) PP_0159family III CoA‑transferase
Reads supporting (aligned to +/- strand):  ref base A (21/21);  new base T (2/2);  total (23/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01

CCGATATTCTGACCGGGTTGTATTCCGCGGTCGCCATGCTGGCTGCCCTCGCCCATCGGGATCAGACAGGGGTCGGCCAGCATGTCGACATGGCATTGCTTGATGTGCAGGTGGCCTGTCTGGCGAACCAGGCGATGAACTACCTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCAGCCG  >  NC_002947/170131‑170423
                                                                                                                                                  |                                                                                                                                                  
ccGATATTCTGACCGGGTTGTATTCCGCGGTCGCCATGCTGGCTGCCCTCGCCCATCGGGATCAGACAGGGGTCGGCCAGCATGTCGACATGGCATTGCTTGATGTGCAGGTGGCCTGTCTGGCGAACCAGGCGATGAACTACCTGAc                                                                                                                                                   >  6:354898/1‑148 (MQ=255)
      ttCTGACCGGGTTGTATTCCGCGGTCGCCATGCTGGCTGCCCTCGCCCATCGGGATCAGACAGGGGTCGGCCAGCATGTCGACATGGCATTGCTTGATGTGCAGGTGGCCTGTCTGGCGAACCAGGCGATGAACTACCTGACCACGGGc                                                                                                                                            >  5:256136/1‑149 (MQ=255)
                      ttCCGCGGTCGCCATGCTGGCTGCCCTCGCCCATCGGGATCAGACAGGGGTCGGCCAGCATGTCGACATGGCATTGCTTGATGTGCAGGTGGCCTGTCTGGCGAACCAGGCGATGAACTACCTGACCACGGGCACCCCTCCCCGTCGGc                                                                                                                            >  4:343155/1‑149 (MQ=255)
                       tCCGCGGTCGCCATGCTGGCTGCCCTCGCCCATCGGGATCAGACAGGGGTCGGCCAGCATGTCGACATGGCATTGCTTGATGTGCAGGTGGCCTGTCTGGCGAACCAGGCGATGAACTACCTGACCACGGGCACCCCTCCCCGTCGGCt                                                                                                                           >  1:275429/1‑149 (MQ=255)
                                tccATGCTGGCTGCCCTCGCCCATCGGGATCAGACAGGGGTCGGCCAGCATGTCGACATGGCATTGCTTGATGTGCAGGTGGCCTGTATGGCGAACCAGGCGATGAACTACCTGTCCACGGGCACCCCTCCCAGTCGGCTGGGTAATgc                                                                                                                  >  7:110649/2‑149 (MQ=255)
                                gCCATGCTGGCTGCCCTCGCCCATCGGGATCAGACAGGGGTCGGCCAGCATGTCGACATGGCATTGCTTGATGTGCAGGTGGCCTGTCTGGCGAACCAGGCGATGAACTACCTGTCCACGGGCACCCCTCCCCGTCGGCTGGGTAATgc                                                                                                                  >  3:155391/1‑149 (MQ=255)
                                gCCATGCTGGCTGCCCTCGCCCATCGGGATCAGACAGGGGTCGGCCAGCATGTCGACATGGCATTGCTTGATGTGCAGGTGGCCTGTCTGGCGAACCAGGCGATGAACTACCTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATgc                                                                                                                  <  3:237824/149‑1 (MQ=255)
                                 ccATGCTGGCTGCCCTCGCCCATCGGGATCAGACAGGGGTCGGCCAGCATGTCGACATGGCATTGCTTGATGTGCAGGTGGCCTGTCTGGCGAACCAGGCGATGAACTACCTGACCACGGGCACCCCTCCCCGTCGGCt                                                                                                                           <  5:144385/139‑1 (MQ=255)
                                 ccATGCTGGCTGCCCTCGCCCATCGGGATCAGACAGGGGTCGGCCAGCATGTCGACATGGCATTGCTTGATGTGCAGGTGGCCTGTCTGGCGAACCAGGCGATGAACTACCTGACCACGGGCACCCCTCCCCGTCGGCt                                                                                                                           >  6:144385/1‑139 (MQ=255)
                                 ccATGCTGGCTGCCCTCGCCCATCGGGATCAGACAGGGGTCGGCCAGCATGTCGACATGGCATTGCTTGATGTGCAGGTGGCCTGTCTGGCGAACCAGGCGATGAACTACCTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATgcg                                                                                                                 >  6:309337/1‑149 (MQ=255)
                                   aTGCTGGCTGCCCTCGCCCATCGGGATCAGACAGGGGTCGGCCAGCATGTCGACATGGCATTGCTTGATGTGCAGGTGGCATGTCTGGCGAACCAGGCGAGGAACTACCTGAACACGGGCACCCATACCCGTCGGCTGGGTAATGCGCa                                                                                                               >  6:151327/1‑149 (MQ=255)
                                      ctggctgCCCTCGCCCATCGGTATCCGACAGTGGTCGGCCAGCATGTCGACATGGCATTGCTTGATGTGCAGGTGGCCTGTCTGGCGAACCAGGCGTTGAACTACCTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATGCGCATcc                                                                                                            <  2:332653/149‑1 (MQ=255)
                                         gctgCCCTCGCCCATCGGGATCTGACAGGGGTCGGCCAGCATGTCGACATGGCATTGCTTGATGTGCAGGTGGCCTGTCTGGCGAACCAGGCGATGAACTACCTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATGCGCATCCt                                                                                                           <  4:158138/147‑1 (MQ=255)
                                          ctgCCCTCGCCCATCGGGATCAGACAGGGGTCGGCCAGCATGTCGACATGGCATTGCTTGATGTGCAGGTGGCCTGTCTGGCGAACCAGGCGATGAACTACCTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATGCGCATCCTAat                                                                                                        <  5:354898/149‑1 (MQ=255)
                                                      aTCGGGATCAGACAGGGGTCGGCCAGCATGTCGACATGGCATTGCTTGATGTGCAGGTGGCCTGTCTGGCGAACCAGGCGATGAACTACCTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTAc                                                                                            <  6:256136/149‑1 (MQ=255)
                                                       tCGGGATCAGACAGGGGTCGGCCAGCATGTCGACATGGCATTGCTTGATGTGCAGGTGGCCTGTCTGGCGAACCAGGCGATGAACTACCTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTAc                                                                                            >  3:272212/1‑148 (MQ=255)
                                                           gATCAGACAGGGGTCGGCCAGCATGTCGACATGGCATTGCTTGATGTGCAGGTGGCCTTTCTGGCGAACCAGGCGATGAACTACCTGACCACggg                                                                                                                                             <  4:269005/95‑1 (MQ=255)
                                                           gATCAGACAGGGGTCGGCCAGCATGTCGACATGGCATTGCTTGATGTGCAGGTGGCCTGTCTGGCGAACCAGGCGATGAACTACCTGACCACggg                                                                                                                                             >  3:269005/1‑95 (MQ=255)
                                                               aGACAGGGGTCGGCCAGCATGTCGACATGGCATTGCTTGATGTGCAGGTGGCCTGTCTGGCGAACCAGGCGATGAACTACCTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTACCAGGACttt                                                                                   <  4:272212/149‑1 (MQ=255)
                                                                      ggTCGGCCAGCATGTCGACATGGCATTGCTTGATGTGCAGGTGGCCTGTCTGGCGAACCAGGCGATGAACTACCTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGACgg                                                                            <  8:40353/149‑1 (MQ=255)
                                                                          ggCCAGCATGTCGACATGGCATTGCTTGATGTGCAGGTGGCCTGTCTGGCGAACCAGGCGATGAACTACCTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGACGGCAg                                                                         <  3:343155/148‑1 (MQ=255)
                                                                                cATGTCGACATGGCATTGCTTGATGTGCAGGTGGCCTGTCTGGCGAACCAGGCGATGAACTACCTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGACGTCAGATGGCGa                                                                  <  5:88060/149‑1 (MQ=255)
                                                                                      gACATGGCATTGCTTGATGTGCAGGTGGCCTGTCTGGCGAACCAGGCGATGAACTACCTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGACGGCAGATGGCGATTTCAt                                                            <  1:180949/149‑1 (MQ=255)
                                                                                            gCATTGCTTGATGTGCAGGTGGCCTGTCTGGCGAACCAGGCGATGAACTACCTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTa                                                       <  5:309337/148‑1 (MQ=255)
                                                                                            gCATTGCTTGATGTGCAGGTGGCCTGTCTGGCGAACCAGGCGATGAACTACCTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTAc                                                      >  5:324227/1‑149 (MQ=255)
                                                                                             cATTGCTTGATGTGCAGGTGGCCTGTCTGGCGAACCAGGCGATGAACTACCTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTAcc                                                     >  7:285337/1‑149 (MQ=255)
                                                                                                 gCTTGATGTGCAGGTGGCCTGTCTGGCGAACCAGGCGATGAACTACCTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTc                                                  <  3:338426/148‑1 (MQ=255)
                                                                                                  cTTGATGTGCAGGTGGCCTGTCTGGCGAACCAGGCGATGAACTACCTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCg                                                 <  6:324227/148‑1 (MQ=255)
                                                                                                   ttGATGTGCAGGTGGCCTGTCTGGCGAACCAGGCGATGAACTACCTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATg                                                                                                                   >  2:123520/1‑81 (MQ=255)
                                                                                                       tgtgCAGGTGGCCTGTCTGGCGAACCAGGCGATGAACTACCTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAAt                                            >  4:123732/1‑148 (MQ=255)
                                                                                                            aGGTGGCCTGTCTGGCGAACCAGGCGATGAACTACCTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGc                                      <  4:332422/149‑1 (MQ=255)
                                                                                                                   cTGTCTGGCGAACCAGGCGATGAACTACCTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTcc                               >  8:304768/1‑149 (MQ=255)
                                                                                                                      tCTGGCGAACCAGGCGATGATCTACCTGTCCACGGGCACCCCTCCGAGTCGGCTTGGTAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGACGGCAGATTGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCg                              <  8:110649/147‑1 (MQ=255)
                                                                                                                      tCTGGCGAACCAGGCGATGAACTACCTGTCCACGGGCACCCCTCCCCGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCg                              <  4:155391/147‑1 (MQ=255)
                                                                                                                        tGGCGAACCAGGCGATGAACTACCTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTACCa                                                                                          >  3:103589/1‑85 (MQ=255)
                                                                                                                        tGGCGAACCAGGCGATGAACTACCTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTACCa                                                                                          <  4:103589/85‑1 (MQ=255)
                                                                                                                          gCGAACCAGGCGATGAACTACCTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGtt                        <  8:114135/149‑1 (MQ=255)
                                                                                                                          gCGAACCAGGAGATGAACTACCTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTACAAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGta                        >  2:21692/1‑148 (MQ=255)
                                                                                                                              aCCATGAGATGAACTACCTGAGCTCGGGCAACCCTCCCCGTAGGCTGGGTAATGCGCATCATAATATAGTCCCTTACCAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCt                    <  7:136237/149‑1 (MQ=255)
                                                                                                                                 aGGCGATGAACTACCTGACCACGGGTACCCCTCCCCGTCGGCTGGGGAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGCCGGCAGATGGCGAGTGCATTCTTACCGTCGGTAATGACACCCAGTTCCGCAAGTTCGCTGAg                 >  2:205938/1‑149 (MQ=255)
                                                                                                                                 aGGCGATGAACTACCTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAg                 <  8:285337/149‑1 (MQ=255)
                                                                                                                                     gATGAACTACCTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTgg             >  5:92472/1‑149 (MQ=255)
                                                                                                                                            tACCTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCggg        >  2:42252/1‑147 (MQ=255)
                                                                                                                                              ccTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCa      <  6:92472/147‑1 (MQ=255)
                                                                                                                                              ccTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCAGc    >  4:139375/1‑149 (MQ=255)
                                                                                                                                                 gACCACGGGCACCCCTCCCCGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCTCTGAGGTGTCAGGGCAGCCg  >  8:120641/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CCGATATTCTGACCGGGTTGTATTCCGCGGTCGCCATGCTGGCTGCCCTCGCCCATCGGGATCAGACAGGGGTCGGCCAGCATGTCGACATGGCATTGCTTGATGTGCAGGTGGCCTGTCTGGCGAACCAGGCGATGAACTACCTGACCACGGGCACCCCTCCCCGTCGGCTGGGTAATGCGCATCCTAATATAGTCCCTTACCAGGACTTTCCGACGGCAGATGGCGATTTCATTCTTACCGTCGGTAATGACAGCCAGTTCCGCAAGTTCGCTGAGGTGGCCGGGCAGCCG  >  NC_002947/170131‑170423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑