Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,575,198	G→A	9.1%	intergenic (‑184/+26)	pcaP ← / ← galP‑II	porin/porin‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,575,198	0	G	A	9.1%	217.3 / 9.3	77	intergenic (‑184/+26)	pcaP/galP‑II	porin/porin‑like protein
Reads supporting (aligned to +/- strand):  ref base G (38/32);  new base A (3/4);  total (41/36)
Fisher's exact test for biased strand distribution p-value = 6.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.10e-01

GATAGTGATCAGGATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGT‑TGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCTGCCCGAC  >  NC_002947/1575054‑1575344                                                                                                                                                  |                                                                                                                                                   gATAGTGATCAGGATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGT‑TGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTa                                                                                                                                                >  8:244954/1‑149 (MQ=255)         tCAGGATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGT‑TGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTCTTCGCGGATAAACCCGCGaa                                                                                                                                        >  7:81714/1‑149 (MQ=255)           aGGATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGT‑TGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGaa                                                                                                                                        >  6:263786/1‑147 (MQ=255)            ggATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGT‑TGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTCTTCGCGGATAAACCCGCGAatag                                                                                                                                     <  8:81714/149‑1 (MQ=255)             gATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGT‑TGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAgg                                                                                                                                    <  5:263786/149‑1 (MQ=255)              aTAGCCTGCAGTCACCACCTTCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGT‑TGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTCTTCGCGGATAAACCCGCGAATAGGc                                                                                                                                   <  4:159112/149‑1 (MQ=255)                  ccTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGT‑TGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGACTATTCGCTGGTTTATCCGCGAAGAGGCCAAt                                                                                                                               >  5:345741/1‑149 (MQ=255)                   cTGCAGTCACCTCCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGTCAATTCATGGCAGGGTTTTCATCACGCTGT‑TGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTCTTCGCGGATAAACCCGCGAATAGGCCaga                                                                                                                               <  7:329875/148‑3 (MQ=38)                     gCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGT‑TGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAAtt                                                                                                                              >  2:175748/1‑147 (MQ=255)                      cAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGT‑TGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAg                                                                                                                           <  3:187237/149‑1 (MQ=255)                                       agctcagcGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGT‑TGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCg                                                                                                          <  7:244954/149‑1 (MQ=255)                                        gctcagcGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGT‑TGCCAATGATGCCTTCAAAAGGGATGGCGTCTGGCCTATTCGCGGGTTTCTCCGCGAAGAGGCCAATTGAGTCAAAGAAGATGCAGCg                                                                                                          >  8:297023/1‑148 (MQ=255)                                         ctcagcGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGT‑TGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGc                                                                                                                                   >  1:281609/1‑122 (MQ=255)                                         ctcagcGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGT‑TGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGc                                                                                                                                   <  2:281609/122‑1 (MQ=255)                                          tcagcGAATGGCGGCAGCTCGCACTTGGCAATTCATGGCAGGGTTTTCATCACGCTGT‑TGCCAATGATGCCCTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGa                                                                                                                            <  6:127575/128‑1 (MQ=255)                                            agcGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGT‑TGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATaa                                                                                                     <  2:338284/149‑1 (MQ=255)                                            agcGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGT‑TGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATa                                                                                                      >  8:347415/1‑148 (MQ=255)                                             gcGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGT‑TGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAAc                                                                                                    <  1:175748/149‑1 (MQ=255)                                              cGAATGGCGGCAGCTCGCACTGGTCAATTAATGTCAGGGTTTTCATCACGCTGT‑TGCCAATGATGCCTTCAATAGGGAGGGCGTCTTGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACt                                                                                                   <  6:345741/149‑1 (MQ=255)                                              cGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGT‑TGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAAc                                                                                                    >  8:202076/1‑148 (MQ=255)                                                    gcggcAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGT‑TGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACaa                                                                                              <  3:136924/148‑1 (MQ=255)                                                       gcAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGT‑TGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAg                                                                                                            <  5:232319/131‑1 (MQ=255)                                                       gcAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGT‑TGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAg                                                                                                            >  6:232319/1‑131 (MQ=255)                                                       gcAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGT‑TGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCaa                                                                                          <  3:188096/149‑1 (MQ=255)                                                        cAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGTGTGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTGGCGGGt                                                                                                                                                   >  1:76904/1‑92 (MQ=255)                                                        cAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGT‑TGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGt                                                                                                                                                   <  2:76904/91‑1 (MQ=255)                                                          gCTCGCACTGGTCAATTCATTGCAGGGTTTTCATCACGCTGT‑TGCCAATGATGCCTTCTAAAGGGAGGGCGTCTGGCCTATTCGCTGGTTTATGCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCg                                                                                       <  7:309749/149‑1 (MQ=255)                                                            tCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGT‑TGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCa                                                                                                                                 <  5:143972/105‑1 (MQ=255)                                                            tCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGT‑TGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCa                                                                                                                                 >  6:143972/1‑105 (MQ=255)                                                            tCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGT‑TGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGt                                                                                     >  5:360044/1‑149 (MQ=255)                                                            gcGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGT‑TGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGt                                                                                     <  7:297023/148‑1 (MQ=255)                                                               cACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGT‑TGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTtct                                                                                  <  8:49491/149‑1 (MQ=255)                                                                 cTGGGCAATTCATGGCAGGGTTTTCATCACGCTGT‑TGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCa                                                                                <  7:347415/149‑1 (MQ=255)                                                                  tGGGCAATTCATGGCAGGGTTTTCATCACGCTGT‑TGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCAt                                                                               >  6:313828/1‑149 (MQ=255)                                                                  tGGGCAATTCATGGCAGGGTTTTCATCACGCTGT‑TGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCAt                                                                               >  8:40233/1‑149 (MQ=255)                                                                       aaTTCATGGCAGGGTTTTCATCACGCTGT‑TGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAAc                                                                          >  4:138098/1‑149 (MQ=255)                                                                          tCATGGCAGGGTTTTCATCACGCTGT‑TGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAAcgcg                                                                       >  7:41044/1‑149 (MQ=255)                                                                             tGGCAGGGTTTTCATCACGCTGT‑TGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTg                                                                    <  8:23274/149‑1 (MQ=255)                                                                             tGGCAGGGTTTTCATCACGCTGT‑TGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTg                                                                    >  7:23274/1‑149 (MQ=255)                                                                               gCAGGGTTTTCATCACGCTGT‑TGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAAGTCACAATCAAGCGGTTCTCATCGACCGCGTTgtt                                                                  >  1:175577/1‑147 (MQ=255)                                                                                 aGGGTTTTCATCACGCTGT‑TGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAg                                                                                                            <  7:273543/105‑1 (MQ=255)                                                                                 aGGGTTTTCATCACGCTGT‑TGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAg                                                                                                            >  8:273543/1‑105 (MQ=255)                                                                                 aGGGTTTTCATCACGCTGT‑TGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGc                                                                                                           <  4:254337/106‑1 (MQ=255)                                                                                 aGGGTTTTCATCACGCTGT‑TGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGc                                                                                                           >  3:254337/1‑106 (MQ=255)                                                                                 aGGGTTTTCATCACGCTGT‑TGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGc                                                                >  8:297939/1‑149 (MQ=255)                                                                                             aCGCTGT‑TGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGa                                                     <  6:360044/148‑1 (MQ=255)                                                                                               gCTGT‑TGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGc                                                  <  5:313828/149‑1 (MQ=255)                                                                                                  gT‑TGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATg                                               >  5:57192/1‑149 (MQ=255)                                                                                                   t‑tGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATg                                               <  1:357015/148‑1 (MQ=255)                                                                                                      gCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTc                                            >  4:268283/1‑149 (MQ=255)                                                                                                       ccAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTc                                            >  5:67317/1‑148 (MQ=255)                                                                                                        cAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTg                                                                    <  6:373100/123‑1 (MQ=255)                                                                                                        cAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTg                                                                    >  5:373100/1‑123 (MQ=255)                                                                                                               gCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTgcgc                                   >  3:326563/1‑149 (MQ=255)                                                                                                                 cTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCa                                 >  4:239512/1‑149 (MQ=255)                                                                                                                  ttCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCAt                                >  8:329122/1‑149 (MQ=255)                                                                                                                    cAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCAt                                <  7:202076/147‑1 (MQ=255)                                                                                                                    cAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCAt                                <  3:268283/147‑1 (MQ=255)                                                                                                                          ggagggCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTgc                                     <  7:308702/136‑1 (MQ=255)                                                                                                                          ggagggCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTgc                                     >  8:308702/1‑136 (MQ=255)                                                                                                                                 gTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTcc                 >  6:275831/1‑149 (MQ=255)                                                                                                                                   ttGGCCTCTTCGCGGATAAACCCGCGAATAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCtt               >  8:208694/2‑149 (MQ=39)                                                                                                                                   cTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCtt               >  2:144201/1‑149 (MQ=255)                                                                                                                                     ggCCTCTTCGCGGATAAACCCGCGAATAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACATGTCCtt               <  3:21013/147‑1 (MQ=255)                                                                                                                                     ggCCTCTTCGCGGATAAACCCGCGAATAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCtt               >  4:21013/1‑147 (MQ=255)                                                                                                                                      gCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCtt               <  7:40233/146‑1 (MQ=255)                                                                                                                                      gCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGa             <  7:297939/148‑1 (MQ=255)                                                                                                                                      gCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGa             <  6:67317/148‑1 (MQ=255)                                                                                                                                        cTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTAAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGATGAATTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGc          >  8:181237/1‑149 (MQ=255)                                                                                                                                         tATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCt         >  6:289849/1‑149 (MQ=255)                                                                                                                                              gcgGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCt                <  8:271334/137‑1 (MQ=255)                                                                                                                                              gcgGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCt                >  7:271334/1‑137 (MQ=255)                                                                                                                                              gcgGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGa             >  6:209729/1‑140 (MQ=255)                                                                                                                                              gcgGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGa             <  5:209729/140‑1 (MQ=255)                                                                                                                                              gcgGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCTGccc     >  8:285822/1‑148 (MQ=255)                                                                                                                                              gcgGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCTGccc     >  2:232880/1‑148 (MQ=255)                                                                                                                                               cgGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCTGCCCGa   >  8:248312/1‑149 (MQ=255)                                                                                                                                                gggTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCTGCCCGAc  >  2:204625/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                   GATAGTGATCAGGATAGCCTGCAGTCACCACCATCATCAGCTCAGCGAATGGCGGCAGCTCGCACTGGGCAATTCATGGCAGGGTTTTCATCACGCTGT‑TGCCAATGATGCCTTCAAAAGGGAGGGCGTCTGGCCTATTCGCGGGTTTATCCGCGAAGAGGCCAATTGAGTCACAGAAGATTCAGCGGATAACTCACAATCAAGCGGTTCTCATCGAACGCGTTGGTGCTGAAGTCGCGACGCATGGTCGAGTTGCGCCATTTCAGCGACAGGTCCTTGAAGCTGCCCGAC  >  NC_002947/1575054‑1575344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑