Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,632,772 A→T 5.5% Y509F (TAT→TTT)  lepA → elongation factor 4

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,632,7720AT 5.5% 223.1 / 2.9 72Y509F (TAT→TTT) lepAelongation factor 4
Reads supporting (aligned to +/- strand):  ref base A (34/34);  new base T (2/2);  total (36/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

CGCCTCAAATCCACCAGCCGCGGCTATGCTTCGCTGGATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGCCAAGTGCTACGG  >  NC_002947/1632624‑1632919
                                                                                                                                                    |                                                                                                                                                   
cGCCTCAAATCCACCAGCCGCGGCTATGCTTCGCTGGATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCta                                                                                                                                                     >  6:116789/1‑149 (MQ=255)
  ccTCAAATCCACCAGCCGCGGCTATGCTTCGCTGGATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGGCGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCtat                                                                                                                                                    >  1:246702/1‑148 (MQ=255)
  ccTCAAATCCACCAGCCGCGGCTATGCTTCGCTGGATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCtata                                                                                                                                                   <  1:159533/149‑1 (MQ=255)
      aaaTCCACCAGCCGCGGCTATGCTTCGCTGGATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTTATCGTGCACCGCGACAACGCGGCCta                                                                                                                                                     >  6:380707/1‑143 (MQ=255)
      aaaTCCACCAGCCGCGGCTATGCTTCGCTGGATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATaaa                                                                                                                                                 <  2:75365/147‑1 (MQ=255)
      aaaTCCACCAGCCGCGGCTATGCTTCGCTGGATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCATGCACCGCGACAACGCGGCCTATAAAgg                                                                                                                                               >  1:25706/1‑149 (MQ=255)
      aaaTCCACCAGCCGCGGCTATGCTTCGCTGGATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGAGACAAGGTCGATGCCCTGGCATTTATCGTGCACCGCGACAACGCGGCCta                                                                                                                                                     <  5:380707/143‑1 (MQ=255)
       aaTCCACCAGCCGCGGCTATGCTTCGCTGGATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGc                                                                                                                                              <  5:116789/149‑1 (MQ=255)
        aTCCACCAGCCGCGGCTATGCTTCGCTGGATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGcc                                                                                                                                             >  6:173232/1‑149 (MQ=255)
         tCCACCAGCCGCGGCTATGCTTCGCTGGATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCg                                                                                                                                            >  7:342637/1‑149 (MQ=255)
           caccaGCCGCGGCTATGCTTCGCTGGATTATCATTTCGATCGCTACCAGTCGGTCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTTTATAGGCCgcg                                                                                                                                          <  3:320835/149‑3 (MQ=255)
           caccaGCCGCGGCTATGCTTCGCTGGATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGt                                                                                                                                           <  4:165913/148‑1 (MQ=255)
            cccaGCCGCGGCTATGCTTCGCTGGATTATCCTTTCGCTCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGCTCAACGGCCACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTTTATAGGCCgcgt                                                                                                                                         <  3:70222/148‑4 (MQ=255)
               aGCCGCGGCTATGCTTCGCTGGATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGtt                                                                                                                                      <  5:173232/149‑1 (MQ=255)
               aGCCGCGGCTATGCTTCGCTGGATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGtt                                                                                                                                      <  3:118813/149‑1 (MQ=255)
                gCCGCGGCTATGCTTCGCTGGATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCAGTGCGtt                                                                                                                                      >  6:261242/1‑148 (MQ=255)
                   gcgGCTATGCTTCGCTGGATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGAcc                                                                                                                                  >  2:286911/1‑149 (MQ=255)
                             ttCGCTGGATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAGGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATg                                                                                                                         >  7:89978/1‑148 (MQ=255)
                               cGCTGGATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGaa                                                                                                                       <  1:286911/148‑1 (MQ=255)
                               cGCTGGATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGaa                                                                                                                       <  7:342620/148‑1 (MQ=255)
                                   ggATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAAc                                                                                                                  >  4:288740/1‑149 (MQ=255)
                                       tATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATg                                                                                                                         <  1:155923/138‑1 (MQ=255)
                                       tATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATg                                                                                                                         >  2:155923/1‑138 (MQ=255)
                                       tATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGAt                                                                                                              >  5:349686/1‑149 (MQ=255)
                                            tttCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAg                                                                                                                                                >  3:346952/1‑110 (MQ=255)
                                            tttCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAg                                                                                                                                                <  4:346952/110‑1 (MQ=255)
                                                  tCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAg                                                                                                    <  8:209048/148‑1 (MQ=255)
                                                       aCCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTc                                                                                              <  6:17530/149‑1 (MQ=255)
                                                       aCCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATATTc                                                                                              >  1:121859/1‑149 (MQ=255)
                                                        ccAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCg                                                                                             >  7:133344/1‑149 (MQ=255)
                                                         cAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCATGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGa                                                                                            <  2:25706/149‑1 (MQ=255)
                                                           gTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAg                                                                                                                      <  2:241023/121‑1 (MQ=255)
                                                           gTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAg                                                                                                                      >  1:241023/1‑121 (MQ=255)
                                                              ggCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGTAGGAACTGATCCCTCGGCAGATGTTCGACGTAg                                                                                       <  8:116155/149‑1 (MQ=255)
                                                              ggCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCAGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAg                                                                                       <  5:261242/149‑1 (MQ=255)
                                                               gCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAg                                                                                       <  8:86536/148‑1 (MQ=255)
                                                               gCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAg                                                                                       >  7:86536/1‑148 (MQ=255)
                                                                        gTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAgg                                                                              >  1:17351/1‑148 (MQ=255)
                                                                             gCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCa                                                                        >  1:51014/1‑149 (MQ=255)
                                                                               tGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGAAGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCAtt                                                                      >  3:76933/1‑149 (MQ=255)
                                                                                 gACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTg                                                                     <  2:51014/148‑1 (MQ=255)
                                                                                  aCGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGAAGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTggc                                                                   <  4:76933/149‑1 (MQ=255)
                                                                                     tGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTggcggc                                                                <  6:349686/149‑1 (MQ=255)
                                                                                       cTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTggcggc                                                                >  1:173182/1‑147 (MQ=255)
                                                                                          aTCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCCGCCAATGGCTGcctg                                                             <  7:321113/147‑3 (MQ=255)
                                                                                              aCGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCAtt                                                       <  3:288740/149‑1 (MQ=255)
                                                                                                  cGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTgcgc                                                   <  8:89978/149‑1 (MQ=255)
                                                                                                        ggTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACaa                                             >  3:139157/1‑149 (MQ=255)
                                                                                                        ggTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACaa                                             >  5:80660/1‑149 (MQ=255)
                                                                                                          tCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAAcc                                           <  2:173182/149‑1 (MQ=255)
                                                                                                             aTGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTc                                        <  4:249395/149‑1 (MQ=255)
                                                                                                             aTGCCCTGGCATTGATCGTACACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGt                                         >  4:225455/1‑148 (MQ=255)
                                                                                                                 ccTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAgg                                    >  5:135641/1‑149 (MQ=255)
                                                                                                                 ccTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAgg                                    >  1:74820/1‑149 (MQ=255)
                                                                                                                 ccTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAgg                                    >  3:354942/1‑149 (MQ=255)
                                                                                                                   tGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGt                                         <  7:7379/142‑1 (MQ=255)
                                                                                                                   tGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGt                                         >  8:7379/1‑142 (MQ=255)
                                                                                                                     gCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTg                                                      <  4:73359/127‑1 (MQ=255)
                                                                                                                     gCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTg                                                      >  3:73359/1‑127 (MQ=255)
                                                                                                                     gCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGgcgc                                 <  4:354942/148‑1 (MQ=255)
                                                                                                                     gCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCt                                <  8:133344/149‑1 (MQ=255)
                                                                                                                       aTTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCt                                >  8:309838/1‑147 (MQ=255)
                                                                                                                       aTTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCAATGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCt                                >  5:317782/1‑147 (MQ=255)
                                                                                                                            tCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACTTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCTGACAACCGTCAAGGCGCTCagaaag                         <  2:246702/149‑1 (MQ=255)
                                                                                                                               tGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAAc                      <  6:317782/149‑1 (MQ=255)
                                                                                                                                   ccGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTa                   <  2:196306/148‑1 (MQ=255)
                                                                                                                                   ccGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTa                   >  1:196306/1‑148 (MQ=255)
                                                                                                                                    cgcgACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACt                 <  8:342637/149‑1 (MQ=255)
                                                                                                                                         cAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGcc             >  5:372118/1‑148 (MQ=255)
                                                                                                                                          aaCGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGcc             >  2:224317/1‑147 (MQ=255)
                                                                                                                                          aaCGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGcc             >  3:77968/1‑147 (MQ=255)
                                                                                                                                            cacgGCCTTTATAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGCCAAGt         >  8:230874/3‑149 (MQ=255)
                                                                                                                                             gcgGCCTATTAAGGTCGTGCGTTGACCGAGAAGATGAAGGTACTGATCCCTCGGCAGATGTTCGACGTAGCGATCGAGGCATCCATTGTCGGAGAGATCATTGCGCTGACAACCTTCAAGGCGCTCATAAAGAACGTACTGGCCAAGTg        <  4:139157/149‑1 (MQ=255)
                                                                                                                                             gcgGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGCCAAGTg        >  6:205312/1‑149 (MQ=255)
                                                                                                                                              cgGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGCCAAGTGc       <  2:74820/149‑1 (MQ=255)
                                                                                                                                                 ccTTTATAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGCCAAGTGCTAc    >  2:115173/1‑149 (MQ=255)
                                                                                                                                                  cTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGCCAAGTGCTAcg   >  2:229194/1‑149 (MQ=255)
                                                                                                                                                   tataAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGCCAAGTGCTAcgg  >  2:4550/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CGCCTCAAATCCACCAGCCGCGGCTATGCTTCGCTGGATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGCCAAGTGCTACGG  >  NC_002947/1632624‑1632919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑