Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,632,775 A→T 5.5% K510I (AAA→ATA)  lepA → elongation factor 4

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,632,7750AT 5.5% 230.0 / 2.7 73K510I (AAA→ATA) lepAelongation factor 4
Reads supporting (aligned to +/- strand):  ref base A (36/33);  new base T (2/2);  total (38/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.30e-01

AAATCCACCAGCCGCGGCTATGCTTCGCTGGATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGCCAAGTGCTACGGCGG  >  NC_002947/1632630‑1632922
                                                                                                                                                 |                                                                                                                                                   
aaaTCCACCAGCCGCGGCTATGCTTCGCTGGATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATaaa                                                                                                                                                    <  2:75365/147‑1 (MQ=255)
aaaTCCACCAGCCGCGGCTATGCTTCGCTGGATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCATGCACCGCGACAACGCGGCCTATAAAgg                                                                                                                                                  >  1:25706/1‑149 (MQ=255)
 aaTCCACCAGCCGCGGCTATGCTTCGCTGGATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGc                                                                                                                                                 <  5:116789/149‑1 (MQ=255)
  aTCCACCAGCCGCGGCTATGCTTCGCTGGATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGcc                                                                                                                                                >  6:173232/1‑149 (MQ=255)
   tCCACCAGCCGCGGCTATGCTTCGCTGGATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCg                                                                                                                                               >  7:342637/1‑149 (MQ=255)
     caccaGCCGCGGCTATGCTTCGCTGGATTATCATTTCGATCGCTACCAGTCGGTCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTTTATAGGCCgcg                                                                                                                                             <  3:320835/149‑3 (MQ=255)
     caccaGCCGCGGCTATGCTTCGCTGGATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGt                                                                                                                                              <  4:165913/148‑1 (MQ=255)
      cccaGCCGCGGCTATGCTTCGCTGGATTATCCTTTCGCTCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGCTCAACGGCCACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTTTATAGGCCgcgt                                                                                                                                            <  3:70222/148‑4 (MQ=255)
         aGCCGCGGCTATGCTTCGCTGGATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGtt                                                                                                                                         <  3:118813/149‑1 (MQ=255)
         aGCCGCGGCTATGCTTCGCTGGATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGtt                                                                                                                                         <  5:173232/149‑1 (MQ=255)
          gCCGCGGCTATGCTTCGCTGGATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCAGTGCGtt                                                                                                                                         >  6:261242/1‑148 (MQ=255)
             gcgGCTATGCTTCGCTGGATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGAcc                                                                                                                                     >  2:286911/1‑149 (MQ=255)
                       ttCGCTGGATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAGGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATg                                                                                                                            >  7:89978/1‑148 (MQ=255)
                         cGCTGGATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGaa                                                                                                                          <  1:286911/148‑1 (MQ=255)
                         cGCTGGATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGaa                                                                                                                          <  7:342620/148‑1 (MQ=255)
                             ggATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAAc                                                                                                                     >  4:288740/1‑149 (MQ=255)
                                 tATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATg                                                                                                                            <  1:155923/138‑1 (MQ=255)
                                 tATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATg                                                                                                                            >  2:155923/1‑138 (MQ=255)
                                 tATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGAt                                                                                                                 >  5:349686/1‑149 (MQ=255)
                                      tttCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAg                                                                                                                                                   <  4:346952/110‑1 (MQ=255)
                                      tttCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAg                                                                                                                                                   >  3:346952/1‑110 (MQ=255)
                                            tCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAg                                                                                                       <  8:209048/148‑1 (MQ=255)
                                                 aCCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTc                                                                                                 <  6:17530/149‑1 (MQ=255)
                                                 aCCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATATTc                                                                                                 >  1:121859/1‑149 (MQ=255)
                                                  ccAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCg                                                                                                >  7:133344/1‑149 (MQ=255)
                                                   cAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCATGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGa                                                                                               <  2:25706/149‑1 (MQ=255)
                                                     gTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAg                                                                                                                         >  1:241023/1‑121 (MQ=255)
                                                     gTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAg                                                                                                                         <  2:241023/121‑1 (MQ=255)
                                                        ggCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGTAGGAACTGATCCCTCGGCAGATGTTCGACGTAg                                                                                          <  8:116155/149‑1 (MQ=255)
                                                        ggCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCAGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAg                                                                                          <  5:261242/149‑1 (MQ=255)
                                                         gCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAg                                                                                          >  7:86536/1‑148 (MQ=255)
                                                         gCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAg                                                                                          <  8:86536/148‑1 (MQ=255)
                                                                  gTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAgg                                                                                 >  1:17351/1‑148 (MQ=255)
                                                                       gCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCa                                                                           >  1:51014/1‑149 (MQ=255)
                                                                         tGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGAAGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCAtt                                                                         >  3:76933/1‑149 (MQ=255)
                                                                           gACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTg                                                                        <  2:51014/148‑1 (MQ=255)
                                                                            aCGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGAAGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTggc                                                                      <  4:76933/149‑1 (MQ=255)
                                                                               tGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTggcggc                                                                   <  6:349686/149‑1 (MQ=255)
                                                                                 cTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTggcggc                                                                   >  1:173182/1‑147 (MQ=255)
                                                                                    aTCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCCGCCAATGGCTGcctg                                                                <  7:321113/147‑3 (MQ=255)
                                                                                        aCGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCAtt                                                          <  3:288740/149‑1 (MQ=255)
                                                                                            cGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTgcgc                                                      <  8:89978/149‑1 (MQ=255)
                                                                                                  ggTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACaa                                                >  3:139157/1‑149 (MQ=255)
                                                                                                  ggTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACaa                                                >  5:80660/1‑149 (MQ=255)
                                                                                                    tCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAAcc                                              <  2:173182/149‑1 (MQ=255)
                                                                                                       aTGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTc                                           <  4:249395/149‑1 (MQ=255)
                                                                                                       aTGCCCTGGCATTGATCGTACACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGt                                            >  4:225455/1‑148 (MQ=255)
                                                                                                           ccTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAgg                                       >  1:74820/1‑149 (MQ=255)
                                                                                                           ccTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAgg                                       >  5:135641/1‑149 (MQ=255)
                                                                                                           ccTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAgg                                       >  3:354942/1‑149 (MQ=255)
                                                                                                             tGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGt                                            <  7:7379/142‑1 (MQ=255)
                                                                                                             tGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGt                                            >  8:7379/1‑142 (MQ=255)
                                                                                                               gCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTg                                                         <  4:73359/127‑1 (MQ=255)
                                                                                                               gCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTg                                                         >  3:73359/1‑127 (MQ=255)
                                                                                                               gCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGgcgc                                    <  4:354942/148‑1 (MQ=255)
                                                                                                               gCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCt                                   <  8:133344/149‑1 (MQ=255)
                                                                                                                 aTTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCt                                   >  8:309838/1‑147 (MQ=255)
                                                                                                                 aTTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCAATGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCt                                   >  5:317782/1‑147 (MQ=255)
                                                                                                                      tCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACTTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCTGACAACCGTCAAGGCGCTCagaaag                            <  2:246702/149‑1 (MQ=255)
                                                                                                                         tGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAAc                         <  6:317782/149‑1 (MQ=255)
                                                                                                                             ccGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTa                      >  1:196306/1‑148 (MQ=255)
                                                                                                                             ccGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTa                      <  2:196306/148‑1 (MQ=255)
                                                                                                                              cgcgACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACt                    <  8:342637/149‑1 (MQ=255)
                                                                                                                                   cAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGcc                >  5:372118/1‑148 (MQ=255)
                                                                                                                                    aaCGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGcc                >  2:224317/1‑147 (MQ=255)
                                                                                                                                    aaCGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGcc                >  3:77968/1‑147 (MQ=255)
                                                                                                                                      cacgGCCTTTATAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGCCAAGt            >  8:230874/3‑149 (MQ=255)
                                                                                                                                       gcgGCCTATTAAGGTCGTGCGTTGACCGAGAAGATGAAGGTACTGATCCCTCGGCAGATGTTCGACGTAGCGATCGAGGCATCCATTGTCGGAGAGATCATTGCGCTGACAACCTTCAAGGCGCTCATAAAGAACGTACTGGCCAAGTg           <  4:139157/149‑1 (MQ=255)
                                                                                                                                       gcgGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGCCAAGTg           >  6:205312/1‑149 (MQ=255)
                                                                                                                                        cgGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGCCAAGTGc          <  2:74820/149‑1 (MQ=255)
                                                                                                                                           ccTTTATAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGCCAAGTGCTAc       >  2:115173/1‑149 (MQ=255)
                                                                                                                                            cTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGCCAAGTGCTAcg      >  2:229194/1‑149 (MQ=255)
                                                                                                                                             tataAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGCCAAGTGCTAcgg     >  2:4550/1‑149 (MQ=255)
                                                                                                                                               taAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGCCAAGTGCTAcggcg   >  4:59337/1‑149 (MQ=255)
                                                                                                                                                aaaGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGCCAAGTGCTAcggcgg  >  6:77734/1‑149 (MQ=255)
                                                                                                                                                aaaGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGCCAAGTGCTAcggcgg  >  6:72513/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
AAATCCACCAGCCGCGGCTATGCTTCGCTGGATTATCATTTCGATCGCTACCAGTCGGCCAACCTGGTCAAGCTGGACGTGCTGATCAACGGCGACAAGGTCGATGCCCTGGCATTGATCGTGCACCGCGACAACGCGGCCTATAAAGGCCGTGCGTTGACCGAGAAGATGAAGGAACTGATCCCTCGGCAGATGTTCGACGTAGCGATCCAGGCAGCCATTGGCGGCCAGATCATTGCGCGGACAACCGTCAAGGCGCTCAGAAAGAACGTACTGGCCAAGTGCTACGGCGG  >  NC_002947/1632630‑1632922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑