Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,635,754 T→A 8.0% intergenic (+100/‑70) era → / → recO GTPase Era/DNA repair protein RecO

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,635,7540TA 8.0% 136.1 / 2.2 50intergenic (+100/‑70)era/recOGTPase Era/DNA repair protein RecO
Reads supporting (aligned to +/- strand):  ref base T (24/22);  new base A (2/2);  total (26/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

CGACGAGCGCGCCCTGCGTTCGCTGGGCTACGGCGACCTGTAAGCCGCGCGCTGGTCCCTGTAGGGGCGGCCTTGTGTCGCGATAGGGCTACGCAGCAGCCCCGGCGATTTATGCTGCGAAGCTAAAAATCTGGGGCCGCTTTGCGGCCCTTTTCGCGACACAAGGCCGCTCCCACAAGGGCCAGTGCAACCCTCGGCGCGAGTGATTTTTAATGGAACAACCTGTCGGTCAGCCAGCCTACGTACTGCACAGCCGTGCCTACAAGGAAACCAGCGCGCTGGTGGACTT  >  NC_002947/1635612‑1635900
                                                                                                                                              |                                                                                                                                                  
cgacgtGCGCGCCCTGCGTTCGCTGGGCTACGGTGACCTGTAAGCGGCGCGCTGGTCCCTGTAGGGGCGGCCTTGTGTCGCGATAGGGCTACGCAGCAGCCCCGGCGATTTATGCTGCGAAGCTAAAAATCTGTGGCCGCTTTGCGGcc                                                                                                                                              <  8:304156/149‑1 (MQ=255)
 gacgaGCGCGCCCTGCGTTCGCTGGGCTACGGCGACCTGTAAGCCGCGCGCTGGTCCCTGTAGGGGCGGCCGTGTGTGGCGATAGGGCTACGCAGCAGCCCCGGCGCTTTATGGTGCGAAGCTAAAAATCTGGGGGCGCGTTGCGGccc                                                                                                                                             >  1:347384/1‑149 (MQ=255)
    gaGCGCGCCCTGCGTTCGCTGGGCTACGGCGACCTGTAAGCCGCGCGCTGGTCCCTGTAGGGGCGGCCTTGTGTCGCGATAGGGCTACGCAGCAGCCCCGGCGATTTATGCTGCGAAGCTAAAAATCTGGGGCCGCAAAGCGGccccag                                                                                                                                          <  5:294217/149‑4 (MQ=255)
      gcgcgcCCTGCGTTCGCTGGGCTACGGCGACCTGTAAGCCGCGCGCTGGTCCCTGTAGGGGCGGCCTTGTGTCGCGATAGGGCTACGCAGCAGCCCCGGCGATTTATGCTGCGAAGCTAAAAATCTGGGGCCGCTTTGCGGCCCTTTTc                                                                                                                                        <  1:66932/149‑1 (MQ=255)
      cgtagcCCAGCGAACGCAGGGCTACGGCGACCTGTAAGCCGCGCGCTGGTCCCTGTAGGGGCGGCCTTTTGTCGCGATAGGGCTACGCAGCAGCCCCGGCGATTTATTCTGCGAATCTAAAAATATTGGGCCGCGTTTCGGCCCttt                                                                                                                                          >  4:343114/5‑147 (MQ=255)
       cgcgcCCTGCGTTCGCTGGGCTACGGCGACCTGTAAGCCGCGCGCTGGTCCCTGTAGGGGCGGCCTTGTGTCGCGATAGGGCTACGCAGCAGCCCCGGCGATTTATGCTGCGAAGCTAAAAATCTGGGGCCGCTTTGCGGCCCTTTTcg                                                                                                                                       >  8:283520/1‑149 (MQ=255)
          gcCCTGCGTTCGCTGGGCTACGGCGACCTGTAAGCCGCGCGCTGGTCCCTGTAGGGGCGGCCTTGTGTCGCGATAGGGCTACGCAGCAGCCCCGGCGATTTATGCTGCGAAGCTAAAAATCTGGGGCCGCTTTGCGGCCCTTTTCGCGa                                                                                                                                    >  7:219997/1‑149 (MQ=255)
                  ttCGCTGGGCTACGGCGACCTGTAAGCCGCGCGCTGGTCCCTGTAGGGGCGGCCTTGTGTGGCGATAGGGCTAAGCAGCAGCCCCGGCGATTTTTGGTGCGAAGCTAAAAATATGGGGCCGCTTTTGGGCCCTTTTCGCGCCACAAGGc                                                                                                                            >  8:33991/1‑149 (MQ=255)
                      cTGGGCTACGGCGACCTGTAAGCCGCGCGCTGGTCCCTGTAGGGGCGGCCTTGTGTCGCGATAGGGCTACGCAGCAGCCCCGGCGATTTATGCTGCGAAGCTAAAAATCTGGGGCCGCTTTGCGGCCCTTTTCGCGACACAACGCCGCt                                                                                                                        >  2:5081/1‑149 (MQ=255)
                           cTACGGCGACCTGTACGCCGCGCGCTGGTCCCTGTAGGGGCGGCCTTGTGTCGCGATAGGGCTACGCAGCAGCCCCGGCGATTTATGCTGCGAAGCTAAAAATCTGGGGCCGCTTTGCGGCCCTTTTCGCGACACAAGGCCGCTCCcac                                                                                                                   <  2:310683/149‑1 (MQ=255)
                              cGGCGACCTGTAAGCCGCGCGCTGGTCCCTGTAGGGGCGGCCTTGTGTCGCGATAGGGCTACGCAGCAGCCCCGGCGATTTATGCTGCGAAGCTAAAAATCTGGGGCCGCTTTGCGGCCCTTTTCGCGACACAAGGCCGCTCCCACAAg                                                                                                                <  7:33991/149‑1 (MQ=255)
                                     cTGTAAGCCGCGCGCTGGTCCCTGTAGGGGCGGCCTTGTGTCGCGATAGGGCTACGCAGCAGCCCCGGCGATTTATGCTGCGAAGCTAAAAATCTGGGGCCGCTTTGCGGCCCTTTTCGCGACACAAGGCCGCTCCCACAAGGGCCAGt                                                                                                         >  4:42061/1‑149 (MQ=255)
                                     cTGTAAGCCGCGCGCGGGTCCCTGTAGGGGGGGCCTGGTGTCGCTATAGGGCTCCGCAGCCGCCCCGGCTATTTATCCTGCGAAGATAAAAATCTGGGGCCGCTTTGCGGCCCTTTTCGCGACACAAGGCCGCTCCCACAAGGGCCAGt                                                                                                         <  2:347384/149‑1 (MQ=37)
                                       gTAAGCCGCGCGCTGGTCCCTGTAGGGGCGGCCTTGTGTCGCGATAGGGCTACGCAGCAGCCCCGGCGATTTATGCTGCGAAGCTAAAAATCTGGGGCCGCTTTGCGGCCCTTTTCGCGACACAAGGCCGCTCCCACAAGGGCCAGTg                                                                                                        >  6:184405/1‑148 (MQ=255)
                                          aGCCGCGCGCTGGTCCCTGTAGGGGCGGCCTTGTGTCGCGATAGGGCTACGCAGCAGCCCCGGCGATTTATGCTGCGAAGCTAAAAATCTGGGGCCGCTTTGCGGCCCTTTTCGCGACACAAGGCCGCTCCCACAAGGGCCAGTGCaa                                                                                                     <  8:70696/148‑1 (MQ=255)
                                            ccGCGCGCTGGTCCCTGTAGGGGCGGCCTTGTGTCGCGATAGGGCTACGCAGCAGCCCCGGCGATTTATGCTGCGAAGCTAAAAATCTGGGGCCGCTTTGCGGCCCTTTTCGCGACACAAGGCCGCTCCCACAAGGGCCAGTGCAAccc                                                                                                  >  1:83804/1‑149 (MQ=255)
                                                      gTCCCTGTAGGGGCGGCCTTGTGTCGCGATAGGGCTACGCAGCAGCCCCGGCGATTTATGCTGCGAAGCTAAAAATCTGGGGCCGCTTTGCGGCCCTTTTCGCGACACAAGGCCGCTCCCACAAGGGCCAGt                                                                                                         <  5:53769/132‑1 (MQ=255)
                                                      gTCCCTGTAGGGGCGGCCTTGTGTCGCGATAGGGCTACGCAGCAGCCCCGGCGATTTATGCTGCGAAGCTAAAAATCTGGGGCCGCTTTGCGGCCCTTTTCGCGACACAAGGCCGCTCCCACAAGGGCCAGTGCAACCCTCGGCGCGa                                                                                         <  7:283520/148‑1 (MQ=255)
                                                          cTGTAGGGGCGGCCTTGTGTGGCGCTAGGGCTACGCAGCAGCCCCGGCGATTTATGCTGCGCAGCTAAAAATCTGGGGCCGCTTTGCCGCCCTTTTCGCGACACCAGGCCGCTCCCCCAAGGGCCAGTGCAACCCTCGGCGCGAGTGAt                                                                                    >  3:116888/1‑149 (MQ=255)
                                                                 gggggcTTTGTGTCGCTATCGGGATACGCAGCCGCCCCGGCTTTTTATGCTGCTAAGCTAAAAATCGGGGGCGGTTTTGCGGCCTTTTTCGCCAAAAATGGCCGCTCCCACAAGGGCCAGTGCAACCCTCGGCGCGAGTGATTTTTAAt                                                                             <  8:120666/146‑1 (MQ=255)
                                                                  gcggcCTTGTGTCGCGATAGGGCTACGCAGCAGCCCCGGCGATTTATGCTGCGAAGCTAAAAATCTGGGGCCGCTTTGCGGCCCTTTTCGCGACACAAGGCCGCTCCCACAAGGGCCAGTGCAACCCTCGGCGCGAGTGATTTTTAATg                                                                            <  3:42061/149‑1 (MQ=255)
                                                                  gcggcCTTGTGTCGCGATAGGGCTACGCAGCAGCCCCGGCGATTTATGCTGCGAAGCTAAAAATCTGGGGCCGCTTTGCGGCCCTTTTCGCGACACAAGGCCGCTCCCACAAGGGCCAGTGCAACCCTCGGCGCGAGTGATTTTTAATg                                                                            >  7:262196/1‑149 (MQ=255)
                                                                    ggcCTTGTGTCGCGATAGGGCTACGCAGCAGCCCCGGCGATTTATGCTGCGAAGCTAAAAATCTGGGGCCGCTTTGCGGCCCTTTTCGCGACACAAGGCCGCTCCCACAAGGGCCAGTGCAACCCTCGGCGCGAGTGATTTTTAATGGa                                                                          <  8:272760/149‑1 (MQ=255)
                                                                      ccTTGTGTCGCGATAGGGCTACGCAGCAGCCCCGGCGATTTATGCTGCGAAGCTAAAAATCTGGGGCCGCTTTGCGGCCCTTTTCGCGACACAAGGCCGATCCCACAAGGGCCAGTGCAACCCTCGGCGCGAGTGATTTTTAATGGaa                                                                         >  6:303214/1‑148 (MQ=255)
                                                                        ttGTGTCGCGATAGGGCTACGCAGCAGCCCCGGCGATTTATGCTGCGAAGCTAAAAATCTGGGGCCGCTTTGCGGCCCTTTTCGCGACACAAGGCCGCTCCCACAAGGGCCAGTGCAACCCTCGGCGCGAGTGATTTTTAATGGaacaa                                                                      >  6:13593/1‑149 (MQ=255)
                                                                          gtgtCGCGATAGGGCTACGCAGCAGCCCCGGCGATTTATGCTGCGAAGCTAAAAATCTGGGGCCGCTTTGCGGCCCTTTTCGCGACACAAGGCCGCTCCCACAAGGGCCAGTGCAACCCTCGGCGCGAGTGATTTTTAATGGaacaac                                                                     <  3:343114/148‑1 (MQ=255)
                                                                          gtgtCGCGATAGGGCTACGCAGCAGCCCCGGCGATTTATGCTGCGAAGCTAAAAATCTGGGGCCGCTTTGCGGCCCTTTTCGCGACACAAGGCCGCTCCCACAAGGGCCAGTGCAACCCTCGGCGCGAGTGATTTTTAATGGaacaac                                                                     <  5:184405/148‑1 (MQ=255)
                                                                           tgtCGCGATAGGGCTACGCAGCAGCCCCGGCGATTTATGCTGCGAAGCTAAAAATCTGGGGCCGCTTTGCGGCCCTTTTCGCGACACAAGGCCGCTCCCACAAGGGCCAGTGCAACCCTCGGCGCGAGTGATTTTTAATGGAACAACCt                                                                   >  7:29437/1‑149 (MQ=255)
                                                                           tgtCGCGATAGGGCTACGCAGCAGACCCGGCGATTTATGATGCGAAGCTAAAAATCTGGGGCCGCTTTGCGGACCTTTTCGCGACACAAGGCCGCTCCCACAAGGGCCAGTGCAACCCTACGCGCGAGTGATTTTTAATGGAACAAACt                                                                   >  4:85529/1‑149 (MQ=38)
                                                                                 gATAGGGCTACGCAGCAGCCCCGGCGATTTATGCTGCGAAGCTAAAAATCTGGGGCCGCTTTGCGGCCCTTTTCGCGACACAAGGCCGCTCCCACAAGGGCCAGTGCAACCCTCGGCGCGAGTGATTTTTAATg                                                                            >  8:216017/1‑134 (MQ=255)
                                                                                 gATAGGGCTACGCAGCAGCCCCGGCGATTTATGCTGCGAAGCTAAAAATCTGGGGCCGCTTTGCGGCCCTTTTCGCGACACAAGGCCGCTCCCACAAGGGCCAGTGCAACCCTCGGCGCGAGTGATTTTTAATg                                                                            <  7:216017/134‑1 (MQ=255)
                                                                                  aTAGGGCTACGCAGCAGCCCCGGCGATTTATGCTGCGAAGCTAAAAATCTGGGGCCGCTTTGCGGCCCTTTTCGCGACACAAGGCCGCTCCCACAAGGGCCAGTGCAACCCTCGGCGCGAGTGATTTTTAATGGAACAACCTGTCGGTc                                                            <  8:219997/149‑1 (MQ=255)
                                                                                            gcagcaGCCCCGGCGATTTATGCTGAGAAGCTAAAAATCTGGGGCCGCTTTGAGGCCCTTTTCGCGACACAAGGCCGCTCCCACAAGGGACAGTGCAACCCTCGGCGCGACTGATTATTAATGTAACAACCTGTCGGTCAGCCAGCCTa                                                  >  4:38917/1‑149 (MQ=38)
                                                                                                     gcGGCGATTTATGCTGCGAAGCTAAAAATCTGGGGCCGCTTTGCGGCCCTTTTCGCGACACAAGGCCGCTCCCACAAGGGCCAGTGCAACCCTCGGCGCGAGTGATTTTTAATGGAACAACCTGTCGGTCAGCCAGCCTACGTACTGca                                         >  2:362225/2‑149 (MQ=255)
                                                                                                     gcGGCGATTTATGCTGCGAAGCTAAAAATCTGGGGCCGCTTTGCGGCCCTTTTCGCGACACAAGGCCGCTCCCACAAGGGCCAGTGCAACCCTCGGCGCGAGTGATTTTTAATGGAACAACCTGTCGGTCAGCCAGCCTACGTACTGc                                          >  2:356558/2‑148 (MQ=255)
                                                                                                        gCGATTTATGCTGCGAAGCTAAAAATCTGGGGCCGCTTTGCGGCCCTTTTCGCGACACAAGGCCGCTCCCACAAGGGCCAGTGCAACCCTCGCCGCGAGTGATTTTTAATCGAACAACCTGTCGCTCAGCCAGCCTACGTACTGCACAg                                      >  4:184632/1‑149 (MQ=255)
                                                                                                        gCGATTTATGCTGCGAAGATAAAAATGTGGGGCGGCTTTGCGGCCGTTTTCGCGACACAAGGCCGCTCCCACAAGGGCCAGTGCAACCCTCGGCGCGAGTGATTTTTAATGGAACAACCTGTCGGTCAGCCAGCCTACGTACTGCACAg                                      <  3:14638/149‑1 (MQ=255)
                                                                                                         ataTTTATGCTGCGAAGCTAAAAATCTGGGGCCGCTTTGCGGCCCTTTTCGCGACACAAGGCCGCTCCCACAATGGCCAGTGCAACCCTCGGCGCGAGTGATTTTTAATGGAACAACCTGTCGGTCAGCCAGCCTACGTACTGCACAGc                                     <  3:38917/147‑1 (MQ=255)
                                                                                                            tttATGCTGCGAAGCTAAAAATCTGGGGCCGCTTTGCGGCCCTTTTCGCGACACAAGGCCGCTCCCACAAGGGCCAGTGCAACCCTCGGCGCGAGTGATTTTTAATGGAACAACCTGTCGGTCAGCCAGCCTACGTACTGCACAGCCGt                                  >  5:188795/1‑149 (MQ=255)
                                                                                                                             aaaaTCTGGGGCCGCTTAGCGGCCCTTTTCGCGACACAAGGCCGCTCCCACAAGGGCCAGTGCAACCCTCGGCGCGAGTGATTTTTAATGGAACAACCTGTCGGTCAGCCAGCCTACGTACTGCACAGCCGTGCCTACAAGGAAACCAg                 <  3:85529/149‑1 (MQ=255)
                                                                                                                              aaaTCTGGGGCCGCTTTGCGGCCCTTTTCGCGACACAAGGCCGCTCCCACAAGGGCCAGTGCAACCCTCGGCGCGAGTGATTTTTAATGGAACAACCTGTCGGTCAGCCAGCCTACGTACTGCACAGCCGTGCCTACAAGGAAACCAgc                <  1:362225/149‑1 (MQ=255)
                                                                                                                              aaaTCTGGGGCCGCTTTGCGGCCCTTTTCGCGACACAAGGCCGCTCCCACAAGGGCCAGTGCAACCCTCGGCGCGAGTGATTTTTAATGGAACAACCTGTCGGTCAGCCAGCCTACGTACTGCACAGCCGTGCCTACAAGGAAACCAgc                <  1:356558/149‑1 (MQ=255)
                                                                                                                               aaTCTGGGGCCGCTTTGCGGCCCTTTTCGCGACACAAGGCCGCTCCCACAAGGGCCAGTGCAACCCTCGGCGCGAGTGATTTTTAATGGAACAACCTGTCGGTCAGCCAGCCTACGTACTGCACAGCCGTGCCTACAAGGAAACCAgcg               >  6:367274/1‑149 (MQ=255)
                                                                                                                                gaaaagggCCGCAAAGCGGCCCTTTTCGCGACACAAGGTCGCTCCCACAAGGGCCAGTGCAACCCTCGGCGCGAGTGATTTTTAATGGAACAACCTGTCGGTCAGCCAGCCTACGTACTGCACAGCCGTGCCTACAAGGAAACCAgcgc              >  4:282246/6‑149 (MQ=255)
                                                                                                                                  aaagggCCGCAAAGCGGCCCTTTTCGCGACACAAGGCCGCTCCCACAAGGGCCAGTGCAACCCTCGGCGCGAGTGATTTTTAATGGAACAACCTGTCGGTCAGCCAGCCTACGTACTGCACAGCCGTGCCTACAAGGAAACCAgcgcgc            >  7:286770/4‑149 (MQ=255)
                                                                                                                                     gggCCGCTTTGCGGCCCTTTTCGCGACACAAGGCCGCTCCCACAAGGGCCAGTGCAACCCTCGGCGCGAGTGATTTTTAATGGAACAACCTGTCGGTCAGCCAGCCTACGTACTGCACAGCCGTGCCTACAAGGAAACCAGCGCGCtg          <  7:10260/148‑1 (MQ=255)
                                                                                                                                       gCCGCTTTGCGGCCCTTTTCGCGACACAAGGCCGCTCCCACAAGGGCCAGTGCAACCCTCGGCGCGAGTGATTTTTAATGGAACAACCTGTCGGTCAGCCAGCCTACGTACTGCACAGCCGTGCCTACAAGGAAACCAGCGCGCtg          <  2:164843/146‑1 (MQ=255)
                                                                                                                                       gCCGCTTTGCGGCCCTTTTCGCGACACAAGGCCGCTCCCACAAGGGCCAGTGCAACCCTCGGCGCGAGTGATTTTTAATGGAACAACCTGTCGGTCAGCCAGCCTACGTACTGCACAGCCGTGCCTACAAGGAAACCAGCGCGCtg          >  1:164843/1‑146 (MQ=255)
                                                                                                                                         cGCTTTGCGGCCCTTTTCGCGACACAAGGCCGCTCCCACAAGGGCCAGTGCAACCCTCGGCGCGAGTGATTTTTAATGGaacaac                                                                     >  6:195567/1‑85 (MQ=255)
                                                                                                                                         cGCTTTGCGGCCCTTTTCGCGACACAAGGCCGCTCCCACAAGGGCCAGTGCAACCCTCGGCGCGAGTGATTTTTAATGGaacaac                                                                     <  5:195567/85‑1 (MQ=255)
                                                                                                                                              tGCGGCCGTTTTCGCGACACAAGGCCGCTCCCACAAGGGCCAGTGCAACCCTCGGCGCGTGTGTTTTTTAATGGAACAAGCTGTCGGTCAGCCAGCCTACGTAGTGCACAGCCGTGCCTACAAGGAAACCAGCGCGCTGGTGGACtt  <  3:184632/147‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
CGACGAGCGCGCCCTGCGTTCGCTGGGCTACGGCGACCTGTAAGCCGCGCGCTGGTCCCTGTAGGGGCGGCCTTGTGTCGCGATAGGGCTACGCAGCAGCCCCGGCGATTTATGCTGCGAAGCTAAAAATCTGGGGCCGCTTTGCGGCCCTTTTCGCGACACAAGGCCGCTCCCACAAGGGCCAGTGCAACCCTCGGCGCGAGTGATTTTTAATGGAACAACCTGTCGGTCAGCCAGCCTACGTACTGCACAGCCGTGCCTACAAGGAAACCAGCGCGCTGGTGGACTT  >  NC_002947/1635612‑1635900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑