Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,641,275 G→C 5.5% T139S (ACC→AGC) ‡ cmoA ← tRNA 5‑methoxyuridine(34)/uridine‑5‑oxyacetic acid(34) metyltransferase CmoA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,641,2750GC 5.5% 226.5 / 4.4 73T139S (ACC→AGC) ‡cmoAtRNA 5‑methoxyuridine(34)/uridine‑5‑oxyacetic acid(34) metyltransferase CmoA
Reads supporting (aligned to +/- strand):  ref base G (29/40);  new base C (2/2);  total (31/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.32e-01

TCCAGGTGCAGTTCATTGAGCAGGTCCTGCTCTTGTTCATCGGCGAAGCGCAGCTTCTCCGACAGAATCAGCGCGCCGCCGGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAGGCGGGCTCGAAGGGCAGGGCAAGGATATCGGCTTCCAGTACTTGCACTGGCAGCAACTCCTGGAACATCGAATCCTGCGCAGTGAGGTACTGGCGGCAGCGCTCGACCATGGCGGCGGAGTTAT  >  NC_002947/1641128‑1641422
                                                                                                                                                   |                                                                                                                                                   
tCCAGGTGCAGTTCATTGAGCAGGTCCTGCTCTTGTTCATCGGCGAAGCGCAGCTTCTCCGACAGAATCAGCGCGCCGCCGGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGt                                                                                                                                                    <  2:188988/149‑1 (MQ=255)
  cAGGTGCAGTTCATTGAGCAGGTCCTGCTCTTGTTCATCGGCGAAGCGCAGCTTCTCCGACAGAATCAGCGCGCCGCCGGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGa                                                                                                                                                  <  5:50388/149‑1 (MQ=255)
   aGGTGCAGTTCATTGAGCAGGTCCTGCTCTTGTTCATCGGCGAAGCGCAGCTTCTCCGACAGAATCAGCGCGCCGCCGGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTg                                                                                                                                                   >  4:1236/1‑147 (MQ=255)
    ggTGCAGTTCATTGAGCAGGTCCTGCTCTTGTTCATCGGCGAAGCGCAGCTTCTCCGACAGAATCAGCGCGCCGCCGGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAg                                                                                                                                                <  3:1236/149‑1 (MQ=255)
     gTGCAGTTCATTGAGCAGGTCCTGCTCTTGTTCATCGGCGAAGCGCAGCTTCTCCGACAGAATCAGCGCGCCGCCGGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGt                                                                                                                                               <  4:75202/149‑1 (MQ=255)
     gTGCAGTTCATTGAGCAGGTCCTGCTCTTGTTCATCGGCGAAGCGCAGCTTCTCCGACAGAATCAGCGCGCCGCCGGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGt                                                                                                                                               <  8:309492/149‑1 (MQ=255)
      tGCAGTTCATTGAGCAGGTCCTGCTCTTGTTCATCGGCGAAGCGCAGCTTCTCCGACAGAATCAGCGCGCCGCCGGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGtt                                                                                                                                              >  2:128105/1‑149 (MQ=255)
       gCAGTTCATTGAGCAGGTCCTGCTCTTGTTCATCGGCGAAGCGCAGCTTCTCCGACAGAATCAGCGCGCCGCCGGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGtt                                                                                                                                              >  2:343314/1‑148 (MQ=255)
        cAGTTCATTGAGCAGGTCCTGCTCTTGTTCATCGGCGAAGCGCAGCTTCTCCGACAGAATCAGCGCGCCGCCGGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCa                                                                                                                                            >  1:93817/1‑149 (MQ=255)
           ttCATTGAGCAGGTCCTGCTCTTGTTCATCGGCGAAGCGCCGCTTCTCCGACAGAATCAGCGCGCCGCCGGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGGGAAGTTCATCg                                                                                                                                         <  1:253962/149‑1 (MQ=255)
           ttCATTGAGCAGGTCCTGCTCTTGTTCATCGGCGAAGCGCAGCTTCTCCGACAGAATCAGCGCGCCGCCGGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCg                                                                                                                                         >  3:311428/1‑149 (MQ=255)
              aTTGAGCAGGTCCTGCTCTTGTTCATCGGCGAAGCGCAGCTTCTCCGACAGAATCAGCGCGCCGCCGGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCa                                                                                                                                      >  3:19169/1‑149 (MQ=255)
              aTTGAGCAGGTCCTGCTCTTGTTCATCGGCGAAGCGCAGCTTCTCCGACAGAATCAGCGCGCCGCCGGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCa                                                                                                                                      >  2:102715/1‑149 (MQ=255)
               ttGAGCAGGTCCTGCTCTTGTTCATCGGCGAAGCGCAGCTTCTCCGACAGAATCAGCGCGCCGCCGGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCAc                                                                                                                                     >  1:160882/1‑149 (MQ=255)
                  aGCAGGTCCTGCTCTTGTTCATCGGCGAAGCGCAGCTTCTCCGACAGAATCAGCGCGCCGCCGGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTa                                                                                                                                   <  5:164682/148‑1 (MQ=255)
                       gTCCTGCTCTTGTTCATCGGCGAAGCGCAGCTTCTCCGACAGAATCAGCGCGCCGCCGGGCACCATCGCCTGGCGGATTCGCCCGACCAGTTCTAGGCGCTGGTCAGGGGCCATGAACTGCAGGGTGAAGTTCATCGCCACTACCGacg                                                                                                                             >  2:312915/1‑147 (MQ=255)
                             ctctTGTTCATCGGCGAAGCGCAGCTTCTCCGACAGAATCAGCGCGCCGCCGGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAGGCGGGCt                                                                                                                       >  5:94643/1‑149 (MQ=255)
                              tctTGTTCATCGGCGAAGCGCAGCTTCTCCGACAGAATCAGCGCGCCGCCGGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAg                                                                                                                              <  5:191178/141‑1 (MQ=255)
                              tctTGTTCATCGGCGAAGCGCAGCTTCTCCGACAGAATCAGCGCGCCGCCGGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAg                                                                                                                              >  6:191178/1‑141 (MQ=255)
                              tctTGTTCATCGGCGAAGCGCAGCTTCTCCGACAGAATCAGCGCGCCGCCGGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAGGCGGGCTc                                                                                                                      >  2:239866/1‑149 (MQ=255)
                                   ttCATCGGCGAAGCGCAGCTTCTCCGACAGAATCAGCGCGCCGCCGGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAGGCGGGCTCGAagg                                                                                                                 <  1:102715/149‑1 (MQ=255)
                                       tCGGCGAAGCGCAGCTTCTCCGACAGAATCAGCGCGCCGCCGGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTa                                                                                                                                   <  5:134027/127‑1 (MQ=255)
                                       tCGGCGAAGCGCAGCTTCTCCGACAGAATCAGCGCGCCGCCGGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTa                                                                                                                                   >  6:134027/1‑127 (MQ=255)
                                       tCGGCGAAGCGCAGCTTCTCCGACAGAATCAGCGCGCCGCCGGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAGGCGGGCTCGAagggcag                                                                                                             <  5:164317/149‑1 (MQ=255)
                                         ggCGATGCGCAGCTTCTCCGACAGAATCCGCGCGCGGGGGGGCAGCAGCGCCTGGGGTATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAAGTGATGGGTGAAGTTCATCGCCACTACCGAGGCGGGCTCGAagggcagg                                                                                                            <  2:160882/148‑1 (MQ=255)
                                         ggCGAAGCGCAGCTTCTCCGACAGAATCAGCGCGCCGCCGGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAGGCGGGCTCGAagggcaggg                                                                                                           >  6:15969/1‑149 (MQ=255)
                                          gCGAAGCGCAGCTTCTCCGACAGAATCAGCGCGCCGCCGGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAGGCGGGCTCGAagggcagggc                                                                                                          <  1:239866/149‑1 (MQ=255)
                                            gAAGCGCAGCTTCTCCGACAGAATCAGCGCGCCGCCGGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAGGCGGGCTCGAAGGGCAGGGCaa                                                                                                        <  8:10665/149‑1 (MQ=255)
                                               gcgcAGCTTCTCCGACAGAATCAGCGCGCCGCCGGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAGGCGGGCTCGAAGGGCAGGGCAAGGa                                                                                                     <  1:128105/149‑1 (MQ=255)
                                                   aGCTTCTCCGACAGAATCAGCGCGCCGCCGGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAGGCGGGCTCGAAGGGCAGGGCAAGGATATc                                                                                                 <  1:82470/149‑1 (MQ=255)
                                                   aGCTTCTCCGACAGAATCAGCGCGCCGCCGGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAGGCGGGCTCGAAGGGCAGGGCAAGGATATc                                                                                                 >  1:352834/1‑149 (MQ=255)
                                                   aGCTTCTCCGACAGAATCAGCGCGCCGCCGGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAGGCGGGCTCGAAGGGCAGGGCAAGGATATc                                                                                                 <  4:36204/149‑1 (MQ=255)
                                                     cTTCTCCGACAGAATCAGCGCGCCGCCGGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAGGCGGGCTCGAAGGGCAGGGCAAGGATATCgg                                                                                               >  8:220118/1‑149 (MQ=255)
                                                        ctcCGACAGAATCAGCGCGCCGCCGGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAGGCGGGCTCGAAGGGCAGGGCAAGGATATCGGCtt                                                                                            >  1:45301/1‑149 (MQ=255)
                                                           ggcttcaaTCAGCGCGCCGCCGGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAGGCGGGCTCGAAGGGCAGGGCAAGGATATCGGCTTCCa                                                                                         <  1:190162/143‑1 (MQ=255)
                                                           cGACAGAATCAGCGCGCCGCCGGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAGGCGGGCTCGAAGGGCAGGGCAAGGATATCGGCTTCCa                                                                                         <  2:93817/149‑1 (MQ=255)
                                                           cGACAGAATCAGCGCGCCGCCGGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAGGCGGGCTCGAAGGGCAGGGCAAGGATATCGGCTTCCa                                                                                         <  1:251724/149‑1 (MQ=255)
                                                            gACAGAATCAGCGCGCCGCCGGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTTCACCCTGCAGTTCATCGc                                                                                                                                        <  8:92164/101‑1 (MQ=38)
                                                            gACAGAATCAGCGCGCCGCCGGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTTCACCCTGCAGTTCATCGc                                                                                                                                        >  7:92164/1‑101 (MQ=38)
                                                            gACAGAATCAGCGCGCCGCCGGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAGGCGGGCTCGAAGGGCAGGGCAAGGATATCGGCTTCCAg                                                                                        >  3:153887/1‑149 (MQ=255)
                                                            gACAGAATCAGCGCGCCGCCGGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAGGCGGACTCGAAGGGCAGGGCAAGGATATCGGCTTcccg                                                                                        >  3:5823/1‑147 (MQ=255)
                                                              cAGAATCAGCGCGCCGCCGGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAGGCGGGc                                                                                                                        <  1:324909/115‑1 (MQ=255)
                                                              cAGAATCAGCGCGCCGCCGGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAGGCGGGc                                                                                                                        >  2:324909/1‑115 (MQ=255)
                                                                 aaTCAGCGCGCCGCCGGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAGGCGGGCTCGAAGGGCAGGGCAAGGATATCGGCTTCCAGTACtt                                                                                   <  4:311428/149‑1 (MQ=255)
                                                                   tCAGCGCGCCGCCGGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAGGCGGGCTCGAAGGGCAGGGCAAGGATATCGGCTTCCAGTACTTg                                                                                  >  1:54674/1‑148 (MQ=255)
                                                                        gcgcCGCCGGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAGGCGGGCTCGAAGGGCAGGGCAAGGATATCGGCTTCCAGTACTTGCACTgg                                                                            <  7:220118/149‑1 (MQ=255)
                                                                            cgccgGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAGGCGGGCTCGAAGGGCAGGGCAAGGATATCGGCTTCCAGTACTTGCACTGgcag                                                                         >  7:77974/1‑148 (MQ=255)
                                                                                   cagcagCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAGGCGGGCTCGAAGGGCAGGGCAAGGATATCGGCTTCCAGTACTTGCACTGGCAGCAACTCCt                                                                 <  2:45301/149‑1 (MQ=255)
                                                                                    agcagcGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAGGCGGGCTCGAAGGGCAGGGCAAGGATATCGGCTTCCAGTACTTGCACTGGCAGCAACTCCTg                                                                <  6:94643/149‑1 (MQ=255)
                                                                                     gcagcGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAGGCGGGCTCGAAGGGCAGGGCAAGGATATCGGCTTCCAGTACTTGCACTGGCAGCAACTCCTgg                                                               <  2:54674/149‑1 (MQ=255)
                                                                                           ccTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAGGCGGGCTCGAAGGGCAGGGCAAGGATATCGGCTTCCAGTACTTGCACTGGCAGCAACTCCTGGAACATc                                                         <  4:19169/149‑1 (MQ=255)
                                                                                                cGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAGGCGGGCTCGAAGGGCAGGGCAAGGATATCGGCTTCCAGTACTTGCACTGGCAGCAACTCCTGGAACATCGAATc                                                    <  5:15969/149‑1 (MQ=255)
                                                                                                  gATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAGGCGGGCTCGAAGGGCAGGGCAAGGATATCGGCTTCCAGTACTTGCACTGGCAGCAACTCCTGGAACATCGAATCCt                                                  <  1:343314/149‑1 (MQ=255)
                                                                                                       gCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAGGCggg                                                                                                                         >  3:236482/1‑73 (MQ=255)
                                                                                                       gCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAGGCggg                                                                                                                         <  4:236482/73‑1 (MQ=255)
                                                                                                       gCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAGGCGGGCTCGAAGGGCAGGGCAAGGATATCGGCTTCCAGTACTTGCACTGGCAGCAACTCCTGGAACATCGAATCCTgcg                                               >  7:69983/1‑147 (MQ=255)
                                                                                                       gCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAGGCGGGCTCGAAGGGCAGGGCAAGGATATCGGCTTCCAGTACTTGCACTGGCAGCAACTCCTGGAACATCGAATCCTgcg                                               >  2:248069/1‑147 (MQ=255)
                                                                                                            aGCTGTTCTCGGCGCTGGTAAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAGGCGGGCTCGAAGGGCAGGGCAAGGATATCGGCTTCCAGTACTTGCACTGGCAGCAACTCCTGGAACATCGAATCCTGCGCAGTGAg                                        <  6:210620/149‑1 (MQ=255)
                                                                                                             gCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAGGCGGGCTCGAAGGGCAGGGCAAGGATATCGGCTTCCAGTACTTGCACTGGCAGCAACTCCTGGAACATCGAATCCTGCGCAGTGAgg                                       >  1:353609/1‑149 (MQ=255)
                                                                                                                        cgcTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAGGCGGGCTCGAagggcagg                                                                                                            <  3:264902/69‑1 (MQ=255)
                                                                                                                        cgcTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAGGCGGGCTCGAagggcagg                                                                                                            >  4:264902/1‑69 (MQ=255)
                                                                                                                              tCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAGGCGGGCTCGAAGGGCAGGGCAAGGATATCGGCTTCCAGTACTTGCACTGGCAGCAACTCCTGGAACATCGAATCCTGCGCAGTGAGGTACTGGCGGCAGCGCTc                      >  3:261674/1‑149 (MQ=255)
                                                                                                                                aGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAGGCGGGCTCGAAGGGCAGGGCAAGGATATCGGCTTCCAGTACTTGCACTGGCAGCAACTCCTGGAACATCGAATCCTGCGCAGTGAGGTACTGGCGGCAGCGCTCg                     <  2:24382/148‑1 (MQ=255)
                                                                                                                                aGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAGGCGGGCTCGAAGGGCAGGGCAAGGATATCGGCTTCCAGTACTTGCACTGGCAGCAACTCCTGGAACATCGAATCCTGCGCAGTGAGGTACTGGCGGCAGCGCTCGa                    <  2:128109/149‑1 (MQ=255)
                                                                                                                                aGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAGGCGGGCTCGAAGGGCAGGGCAAGGATATCGGCTTCCAGTACTTGCACTGGCAGCAACTCCTGGAACATCGAATCCTGCGCAGTGAGGTACTGGCGGCAGCGCTCGa                    <  4:153887/149‑1 (MQ=255)
                                                                                                                                   ggCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAGGCGGGCTCGAAGGGCAGGGCAAGGATATCGGCTTCCAGTACTTGCACTGGCAGCAACTCCTGGAACATCGAATCCTGCGCAGTGAGGTACTggcg                               <  4:264571/135‑1 (MQ=255)
                                                                                                                                   ggCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAGGCGGGCTCGAAGGGCAGGGCAAGGATATCGGCTTCCAGTACTTGCACTGGCAGCAACTCCTGGAACATCGAATCCTGCGCAGTGAGGTACTggcg                               >  3:264571/1‑135 (MQ=255)
                                                                                                                                      gATGAACTTCACCCTGCAGTTCATCGCCCCTACCGAGGCGGGCTCGAAGGGCAGGGCAAGGATATCGGCTTCCAGTACTTGCACTGGCAGCAACTCCTGGAACATCGAATCCTgc                                                >  4:291607/1‑115 (MQ=38)
                                                                                                                                      gATGAACTTCACCCTGCAGTTCATCGCCCCTACCGAGGCGGGCTCGAAGGGCAGGGCAAGGATATCGGCTTCCAGTACTTGCACTGGCAGCAACTCCTGGAACATCGAATCCTgc                                                <  3:291607/115‑1 (MQ=38)
                                                                                                                                          aaCTGCAGGGTGAAGTTCATCGCCACTACCGAGGCGGGCTCGAAGGGCAGGGCAAGGATATCGGCTTCCAGTACTTGCACTGGCAGCAACTCCTGGAACATCGAATCCTGCGCAGTGAGGTACTGGCGGCAGCGCTCGACCATggcgg           <  8:77974/148‑1 (MQ=255)
                                                                                                                                               cAGGGTGAAGTTCATCGCCACTACCGAGGCGGGCTCGAAGGGCAGGGCAAGGATATCGGCTTCCAGTACTTGCACTGGCAGCAACTCCTGGAACATCGAATCCTGCGCAGTGAGGTACTGGCGGCAGCGCTCGACCATGGCGGCGGa       <  8:69983/147‑1 (MQ=255)
                                                                                                                                               cAGGGTGAAGTTCATCGCCACTACCGAGGCGGGCTCGAAGGGCAGGGCAAGGATATCGGCTTCCAGTACTTGCACTGGCAGCAACTCCTGGAACATCGAATCCTGCGCAGTGAGGTACTGGCGGCAGCGCTCGACCATGGCGGCGGAGt     <  4:261674/149‑1 (MQ=255)
                                                                                                                                                aGGGTGAAGTTCATCGCCACTACCGAGGCGGGCTCGAAGGGCAGGGCAAGGATATCGGCTTCCAGTACTTGCACTGGCAGCAACTCCTGGAACATCGAATCCTGCGCAGTGAGGTACTGGCGGCAGCGCTCGACCATGGCGGCGGAGtt    <  1:326516/149‑1 (MQ=255)
                                                                                                                                                   gTGAAGTTCATCGCCACTACCGAGGCGGGCTCGAAGGGCAGGGCAAGGATATCGGCTTCCAGTACTTGCACTGGCAGCAACTCCTGGAACATCGAATCCTGCGCAGTGAGGTACTGGCGGCAGCGCTCGACCATGGCGGCGGAGTTAt  >  7:265419/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TCCAGGTGCAGTTCATTGAGCAGGTCCTGCTCTTGTTCATCGGCGAAGCGCAGCTTCTCCGACAGAATCAGCGCGCCGCCGGGCAGCAGCGCCTGGCGGATTCGCCCGAGCAGTTCTAGGCGCTGGTCAGGGGCGATGAACTGCAGGGTGAAGTTCATCGCCACTACCGAGGCGGGCTCGAAGGGCAGGGCAAGGATATCGGCTTCCAGTACTTGCACTGGCAGCAACTCCTGGAACATCGAATCCTGCGCAGTGAGGTACTGGCGGCAGCGCTCGACCATGGCGGCGGAGTTAT  >  NC_002947/1641128‑1641422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑