Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,696,393 C→G 8.2% S142S (TCC→TCG) ‡ PP_1492 → two‑component system sensor histidine kinase/response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,696,3930CG 8.2% 143.3 / 5.1 49S142S (TCC→TCG) ‡PP_1492two‑component system sensor histidine kinase/response regulator
Reads supporting (aligned to +/- strand):  ref base C (23/22);  new base G (2/2);  total (25/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.96e-01

TTGCAAGGTACCGACCTGCTGATGCGTATTGCCACACCCGGTGATGCCGGGGCGCAGGCGACCTTGCCGGTGTTTCTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGCAAGCGCGCTGGCGAGGGTGCCGAGCGGGTACTGCGTGTCACCGCCGACCG  >  NC_002947/1696250‑1696539
                                                                                                                                               |                                                                                                                                                   
ttGCAAGGTACCGACCTGCTGATGCGTATTGCCACACCCGGTGATGCCGGGGCGCAGGCGACCTTGCCGGTGTTTCTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCGTgc                                                                                                                                                 <  8:77695/148‑1 (MQ=255)
       gTACCGACCTGCTGATGCGTATTGCCACACCCGGTGATGCCGGGGCGCAGGCGACCTTGCCGGTGTTTCTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCAGCGGCGGCACGGATGCCGCCGGTg                                                                                                                                         >  4:296978/1‑149 (MQ=255)
          ccGACCTGCTGATGCGTATTGCCACACCCGGTGATGCCGGGGCGCAGGCGACCTTGCCGGTGTTTCTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCAGCGGCGGCACGGATGCCGCCGGTGGCa                                                                                                                                      <  3:296978/149‑1 (MQ=255)
             aCCTGCTGATGCGTATTGCCACACCCGGTGATGCCGGGGCGCAGGCGACCTTGCCGGTGTTTCTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAAcc                                                                                                                                   >  8:176544/1‑149 (MQ=255)
                  ctgATGCGTATTGCCACACCCGGTGATGCCGGGGCGCAGGCGACCTTGCCGGTGTTTCTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTgc                                                                                                                               <  1:190690/148‑1 (MQ=255)
                    gATGCGTATTGCCACACCCGGTGATGCCGGGGCGCAGGCGACCTTGCCGGTGTTTCTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTgccgc                                                                                                                            >  5:341923/1‑149 (MQ=255)
                      tGCGTATTGCCACACCCGGTGATGCCGGGGCGCAGGCGACCTTGCCGGTGTTTCTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCt                                                                                                                          <  7:290391/149‑1 (MQ=255)
                       gCGTATTGCCACACCCTGTGATGCCGGGGCGCAGGCGACCTTGCCGGTGTTTCTTGCGCAACTGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCGTGTCGTGCCACCGGCGGCATGCGTGCCGCCGCTGGCAACCCTGCCGCCt                                                                                                                          <  7:269577/148‑1 (MQ=255)
                                cacaCCCGGTGATGCCGGGGCGCAGGCGACCTTGCCGGTGTTTCTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATgg                                                                                                                >  3:262967/1‑149 (MQ=255)
                                 acacCCGGTGATGCCGGGGCGCAGGCGACCTTGCCGGTGTTTCTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCCGCATCCGTGCCGCCCCAGGCAACCCTGCCGCCTGCCCCCATgc                                                                                                                >  2:81962/1‑147 (MQ=255)
                                   acCCGGTGATGCCGGGGCGCAGGCGACCTTGCCGGTGTTTCTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCACGGATGCCGCCGGTg                                                                                                                                         >  4:337950/1‑121 (MQ=255)
                                   acCCGGTGATGCCGGGGCGCAGGCGACCTTGCCGGTGTTTCTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCACGGATGCCGCCGGTg                                                                                                                                         <  3:337950/121‑1 (MQ=255)
                                    cccGGTGATGCCGGGGCGCAGGCGACCTTGCCGGTGTTTCTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAAt                                                                                                            <  4:262967/149‑1 (MQ=255)
                                      cGGGGATGCCGGGGCGCAGGAGACCTTGCCGGTGTTTCTTGCGCAAATGGCCAGCATGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGc                                                                                                          <  8:149041/149‑1 (MQ=255)
                                       ggTGATGCCGGGGCGCAGGCGACCTTGCCGGTGTTTCTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCCTCCGTGCCGCCGCTGGCAACCCTGCCGCCGGCCCCCATGGTAATGcc                                                                                                         >  4:45175/1‑149 (MQ=255)
                                                  ggCGCAGGCGACCTTGCCGGTGTTTCTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAaccac                                                                                              >  1:330454/1‑149 (MQ=255)
                                                  ggCGCAGGCGACCTTGCCGGTGTTTCTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAaccac                                                                                              <  3:340564/149‑1 (MQ=255)
                                                        ggCGACCTTGCCGGTGTTTCTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCGTGCCGCCCCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAAc                                                                                        >  8:4624/1‑149 (MQ=255)
                                                         gCGACCTTGCCGGTGTTTCTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAAc                                                                                        >  1:290876/1‑148 (MQ=255)
                                                            aCCTTGCCGGTGTTTCTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCaga                                                                                    <  2:290876/149‑1 (MQ=255)
                                                              cTTGCCGGTGTTTCTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGc                                                                                  >  6:71217/1‑149 (MQ=255)
                                                                 gCCGGTGTTTCTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCGTTCCGCCGCTGGGCACCCTGCCGCCTGCCCCCAAGGTAATGACAGCCGAACCACCGGGACCTGGGCCCg                                                                               >  2:211179/1‑149 (MQ=255)
                                                                     gtgtTTCTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCCTGCCGCCGCTGGCAACCCTGCCCCCTGCCCCCATGGTAATGCCACCCGAACCACCGGCACCAGAGCCCG‑CAcc                                                                          >  2:21334/1‑149 (MQ=255)
                                                                           cTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCt                                                                    >  3:153324/1‑149 (MQ=255)
                                                                                 cAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCGTGCCCCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCCAACCACCGGAACCAGAGCCCG‑AACCCGCCCTgcggcg                                                              >  6:177363/1‑149 (MQ=255)
                                                                                  aaaTGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCCCCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGCACCCCCGGAACCAGAGCCCG‑GACCCGCCCTGCGgcgc                                                             >  2:274591/1‑149 (MQ=255)
                                                                                     tGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAg                                                          <  2:330454/149‑1 (MQ=255)
                                                                                      ggCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCTCCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAgg                                                         >  6:110451/1‑149 (MQ=255)
                                                                                             cTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCCGCGGCATCCGTGCCGCCCCAGGCAACCCAGCCCCCGGCCCCCAAGGTAATTCCAGCCGCACCACCGGAAACAGACCCCC‑ACCCCCCCCAGCGGCGCCAGTCAGGCaa                                                  >  6:41566/1‑149 (MQ=255)
                                                                                                cTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCGCAACCCGCCCTGCGGCGCAAGGCTGGCaa                                                  <  1:21334/147‑1 (MQ=255)
                                                                                                          gcgcCTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGCAAGCGCGCTGGCGAg                                     <  7:176544/149‑1 (MQ=255)
                                                                                                             ccTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGCAAGCGCGCTGGCGAGGGt                                  >  8:117392/1‑149 (MQ=255)
                                                                                                                   ccATGCCTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGCAAGCGCGCTGGCGAGGGTGcc                               <  6:341923/146‑1 (MQ=255)
                                                                                                                    cATGCCTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGCAAGCGCGCTGGCGAGGGTGCCGAGc                           <  5:177363/149‑1 (MQ=255)
                                                                                                                     aTGCCTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGCAAGCGCGCTGGCGAGGGTGCCGAGCg                          <  7:4624/149‑1 (MQ=255)
                                                                                                                        ccTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGCAAGCGCGCTGGCGAGGGTGCCGAGCggg                        <  4:153324/148‑1 (MQ=255)
                                                                                                                        ccTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGCAAGCGCGCTGGCGAGGGTGCCGAGCggg                        <  3:74468/148‑1 (MQ=255)
                                                                                                                            tCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGCAAGCGCGCTGGCGAGGGTGCCGAGCGGGtctgc                   <  7:117392/149‑5 (MQ=255)
                                                                                                                            tCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGCAAGCGCGCTGGCGAGGGTGCCGAGCGGGTACTg                   >  5:175644/1‑149 (MQ=255)
                                                                                                                             cGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGCAAGCGCGCTGGCGAGGGTGCCGAGCGGGTACTGc                  <  6:235056/149‑1 (MQ=255)
                                                                                                                              gTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGCAAGCGCGCTGGCGAGGGTGCCGAGCGGGTACTGc                  <  6:175644/148‑1 (MQ=255)
                                                                                                                              gTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGCAAGCGCGCTGGCGAGGGTGCCGAGCGGGTACTGc                  <  6:5792/148‑1 (MQ=255)
                                                                                                                               tGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGCAAGCGCGCTGGCGAGGGTGCCGAGCGGGTACTGCgt                <  5:71217/149‑1 (MQ=255)
                                                                                                                               tGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGCAAGCGCGCTGGCGAGGGTGCCGAGCGGGTACTGCgt                >  3:309228/1‑149 (MQ=255)
                                                                                                                               tGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGCAAGCGCGCTGGCGAGGGTGCCGAGCGGGTACTGCgt                >  3:314515/1‑149 (MQ=255)
                                                                                                                                  cACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGACCCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGCAAGCGCGCTGGCGAGGGTGCCGAGCGTGTACTGCgtgt              >  5:350560/1‑148 (MQ=255)
                                                                                                                                  cACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGCAAGCGCGCTGGCGAgg                                    >  1:208197/1‑126 (MQ=255)
                                                                                                                                  cACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGCAAGCGCGCTGGCGAgg                                    <  2:208197/126‑1 (MQ=255)
                                                                                                                                             tCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGCAAGCGCGCTGGCGAGGGTGCCGAGCGGGTACTGCGTGTCACCGCCGACCg  >  5:57179/1‑149 (MQ=255)
                                                                                                                                               cGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGc                                                    <  1:225248/97‑1 (MQ=255)
                                                                                                                                               cGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGc                                                    >  2:225248/1‑97 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
TTGCAAGGTACCGACCTGCTGATGCGTATTGCCACACCCGGTGATGCCGGGGCGCAGGCGACCTTGCCGGTGTTTCTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGCAAGCGCGCTGGCGAGGGTGCCGAGCGGGTACTGCGTGTCACCGCCGACCG  >  NC_002947/1696250‑1696539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑