Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,696,403 C→G 7.7% L146V (CTG→GTG)  PP_1492 → two‑component system sensor histidine kinase/response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,696,4030CG 7.7% 159.0 / 5.2 52L146V (CTG→GTG) PP_1492two‑component system sensor histidine kinase/response regulator
Reads supporting (aligned to +/- strand):  ref base C (25/23);  new base G (2/2);  total (27/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01

GTACCGACCTGCTGATGCGTATTGCCACACCCGGTGATGCCGGGGCGCAGGCGACCTTGCCGGTGTTTCTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGCAAGCGCGCTGGC‑GAGGGTGCCGAGCGGGTACTGCGTGTCACCGCCGACCGCCTCAATA  >  NC_002947/1696257‑1696547
                                                                                                                                                  |                                                                                                                                                  
gTACCGACCTGCTGATGCGTATTGCCACACCCGGTGATGCCGGGGCGCAGGCGACCTTGCCGGTGTTTCTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCAGCGGCGGCACGGATGCCGCCGGTg                                                                                                                                                  >  4:296978/1‑149 (MQ=255)
   ccGACCTGCTGATGCGTATTGCCACACCCGGTGATGCCGGGGCGCAGGCGACCTTGCCGGTGTTTCTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCAGCGGCGGCACGGATGCCGCCGGTGGCa                                                                                                                                               <  3:296978/149‑1 (MQ=255)
      aCCTGCTGATGCGTATTGCCACACCCGGTGATGCCGGGGCGCAGGCGACCTTGCCGGTGTTTCTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAAcc                                                                                                                                            >  8:176544/1‑149 (MQ=255)
           ctgATGCGTATTGCCACACCCGGTGATGCCGGGGCGCAGGCGACCTTGCCGGTGTTTCTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTgc                                                                                                                                        <  1:190690/148‑1 (MQ=255)
             gATGCGTATTGCCACACCCGGTGATGCCGGGGCGCAGGCGACCTTGCCGGTGTTTCTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTgccgc                                                                                                                                     >  5:341923/1‑149 (MQ=255)
               tGCGTATTGCCACACCCGGTGATGCCGGGGCGCAGGCGACCTTGCCGGTGTTTCTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCt                                                                                                                                   <  7:290391/149‑1 (MQ=255)
                gCGTATTGCCACACCCTGTGATGCCGGGGCGCAGGCGACCTTGCCGGTGTTTCTTGCGCAACTGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCGTGTCGTGCCACCGGCGGCATGCGTGCCGCCGCTGGCAACCCTGCCGCCt                                                                                                                                   <  7:269577/148‑1 (MQ=255)
                         cacaCCCGGTGATGCCGGGGCGCAGGCGACCTTGCCGGTGTTTCTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATgg                                                                                                                         >  3:262967/1‑149 (MQ=255)
                          acacCCGGTGATGCCGGGGCGCAGGCGACCTTGCCGGTGTTTCTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCCGCATCCGTGCCGCCCCAGGCAACCCTGCCGCCTGCCCCCATgc                                                                                                                         >  2:81962/1‑147 (MQ=255)
                            acCCGGTGATGCCGGGGCGCAGGCGACCTTGCCGGTGTTTCTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCACGGATGCCGCCGGTg                                                                                                                                                  >  4:337950/1‑121 (MQ=255)
                            acCCGGTGATGCCGGGGCGCAGGCGACCTTGCCGGTGTTTCTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCACGGATGCCGCCGGTg                                                                                                                                                  <  3:337950/121‑1 (MQ=255)
                             cccGGTGATGCCGGGGCGCAGGCGACCTTGCCGGTGTTTCTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAAt                                                                                                                     <  4:262967/149‑1 (MQ=255)
                               cGGGGATGCCGGGGCGCAGGAGACCTTGCCGGTGTTTCTTGCGCAAATGGCCAGCATGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGc                                                                                                                   <  8:149041/149‑1 (MQ=255)
                                ggTGATGCCGGGGCGCAGGCGACCTTGCCGGTGTTTCTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCCTCCGTGCCGCCGCTGGCAACCCTGCCGCCGGCCCCCATGGTAATGcc                                                                                                                  >  4:45175/1‑149 (MQ=255)
                                           ggCGCAGGCGACCTTGCCGGTGTTTCTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAaccac                                                                                                       >  1:330454/1‑149 (MQ=255)
                                           ggCGCAGGCGACCTTGCCGGTGTTTCTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAaccac                                                                                                       <  3:340564/149‑1 (MQ=255)
                                                 ggCGACCTTGCCGGTGTTTCTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCGTGCCGCCCCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAAc                                                                                                 >  8:4624/1‑149 (MQ=255)
                                                  gCGACCTTGCCGGTGTTTCTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAAc                                                                                                 >  1:290876/1‑148 (MQ=255)
                                                     aCCTTGCCGGTGTTTCTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCaga                                                                                             <  2:290876/149‑1 (MQ=255)
                                                       cTTGCCGGTGTTTCTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGc                                                                                           >  6:71217/1‑149 (MQ=255)
                                                          gCCGGTGTTTCTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCGTTCCGCCGCTGGGCACCCTGCCGCCTGCCCCCAAGGTAATGACAGCCGAACCACCGGGACCTGGGCCCg                                                                                        >  2:211179/1‑149 (MQ=255)
                                                              gtgtTTCTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCCTGCCGCCGCTGGCAACCCTGCCCCCTGCCCCCATGGTAATGCCACCCGAACCACCGGCACCAGAGCCCG‑CAcc                                                                                   >  2:21334/1‑149 (MQ=255)
                                                                    cTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCt                                                                             >  3:153324/1‑149 (MQ=255)
                                                                          cAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCGTGCCCCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCCAACCACCGGAACCAGAGCCCG‑AACCCGCCCTgcggcg                                                                       >  6:177363/1‑149 (MQ=255)
                                                                           aaaTGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCCCCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGCACCCCCGGAACCAGAGCCCG‑GACCCGCCCTGCGgcgc                                                                      >  2:274591/1‑149 (MQ=255)
                                                                              tGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAg                                                                   <  2:330454/149‑1 (MQ=255)
                                                                               ggCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCTCCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAgg                                                                  >  6:110451/1‑149 (MQ=255)
                                                                                      cTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCCGCGGCATCCGTGCCGCCCCAGGCAACCCAGCCCCCGGCCCCCAAGGTAATTCCAGCCGCACCACCGGAAACAGACCCCC‑ACCCCCCCCAGCGGCGCCAGTCAGGCaa                                                           >  6:41566/1‑149 (MQ=255)
                                                                                         cTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCGCAACCCGCCCTGCGGCGCAAGGCTGGCaa                                                           <  1:21334/147‑1 (MQ=255)
                                                                                                   gcgcCTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGCAAGCGCGCTGGC‑GAg                                             <  7:176544/149‑1 (MQ=255)
                                                                                                      ccTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGCAAGCGCGCTGGC‑GAGGGt                                          >  8:117392/1‑149 (MQ=255)
                                                                                                            ccATGCCTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGCAAGCGCGCTGGC‑GAGGGTGcc                                       <  6:341923/146‑1 (MQ=255)
                                                                                                             cATGCCTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGCAAGCGCGCTGGC‑GAGGGTGCCGAGc                                   <  5:177363/149‑1 (MQ=255)
                                                                                                              aTGCCTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGCAAGCGCGCTGGC‑GAGGGTGCCGAGCg                                  <  7:4624/149‑1 (MQ=255)
                                                                                                                 ccTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGCAAGCGCGCTGGC‑GAGGGTGCCGAGCggg                                <  4:153324/148‑1 (MQ=255)
                                                                                                                 ccTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGCAAGCGCGCTGGC‑GAGGGTGCCGAGCggg                                <  3:74468/148‑1 (MQ=255)
                                                                                                                     tCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGCAAGCGCGCTGGC‑GAGGGTGCCGAGCGGGtctgc                           <  7:117392/149‑5 (MQ=255)
                                                                                                                     tCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGCAAGCGCGCTGGC‑GAGGGTGCCGAGCGGGTACTg                           >  5:175644/1‑149 (MQ=255)
                                                                                                                      cGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGCAAGCGCGCTGGC‑GAGGGTGCCGAGCGGGTACTGc                          <  6:235056/149‑1 (MQ=255)
                                                                                                                       gTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGCAAGCGCGCTGGC‑GAGGGTGCCGAGCGGGTACTGc                          <  6:175644/148‑1 (MQ=255)
                                                                                                                       gTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGCAAGCGCGCTGGC‑GAGGGTGCCGAGCGGGTACTGc                          <  6:5792/148‑1 (MQ=255)
                                                                                                                        tGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGCAAGCGCGCTGGC‑GAGGGTGCCGAGCGGGTACTGCgt                        >  3:309228/1‑149 (MQ=255)
                                                                                                                        tGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGCAAGCGCGCTGGC‑GAGGGTGCCGAGCGGGTACTGCgt                        <  5:71217/149‑1 (MQ=255)
                                                                                                                        tGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGCAAGCGCGCTGGC‑GAGGGTGCCGAGCGGGTACTGCgt                        >  3:314515/1‑149 (MQ=255)
                                                                                                                           cACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGACCCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGCAAGCGCGCTGGC‑GAGGGTGCCGAGCGTGTACTGCgtgt                      >  5:350560/1‑148 (MQ=255)
                                                                                                                           cACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGCAAGCGCGCTGGC‑GAgg                                            <  2:208197/126‑1 (MQ=255)
                                                                                                                           cACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGCAAGCGCGCTGGC‑GAgg                                            >  1:208197/1‑126 (MQ=255)
                                                                                                                                      tCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGCAAGCGCGCTGGC‑GAGGGTGCCGAGCGGGTACTGCGTGTCACCGCCGACCg          >  5:57179/1‑149 (MQ=255)
                                                                                                                                        cGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGc                                                             >  2:225248/1‑97 (MQ=255)
                                                                                                                                        cGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGc                                                             <  1:225248/97‑1 (MQ=255)
                                                                                                                                         gTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGCAAGCGCGCTGGC‑GAGGGTGCCGAGCGGGTACTGCGTGTCACCGCCGACCGCCt       <  6:57179/149‑1 (MQ=255)
                                                                                                                                            ccgccgCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGCAAGCGCGCTGGC‑GAGGGTGCCGAGCGGGTACTGCGTGTCACCGCCGACCGCCTCa     >  4:138141/1‑148 (MQ=255)
                                                                                                                                               ccgcTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGCAAGCGCGCTGGCTGAGGGTGCCGAGCGGGTACTGCGTGTCACCGCCGACCGCCTCAATa  <  1:81962/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GTACCGACCTGCTGATGCGTATTGCCACACCCGGTGATGCCGGGGCGCAGGCGACCTTGCCGGTGTTTCTTGCGCAAATGGCCAGCCTGCTCGACCCGGGCGCCTCTGCCATGCCTGTCGTGCCACCGGCGGCATCCGTGCCGCCGCTGGCAACCCTGCCGCCTGCCCCCATGGTAATGCCAGCCGAACCACCGGAACCAGAGCCCG‑AACCCGCCCTGCGGCGCAAGGCTGGCAAGCGCGCTGGC‑GAGGGTGCCGAGCGGGTACTGCGTGTCACCGCCGACCGCCTCAATA  >  NC_002947/1696257‑1696547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑