Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,754,565	G→A	8.4%	E89E (GAG→GAA)	PP_1556 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,754,565	0	G	A	8.4%	203.0 / 7.0	72	E89E (GAG→GAA)	PP_1556	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (32/33);  new base A (3/3);  total (36/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.88e-01

CTATCAGGACGGCTACTTCGATGCGGATGCGCCAGGTCTATGGATCGTTACTGCCAAGGGCCTGATGCGTAACGCCGAGGGCGGCAAGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCATGAGGAAAAGCCACGGCCCATCCTTCAAGAAG‑GCCGTGATCGAGTTGGATGTATGCCCTTTGTGCCGTGGGAGAGCGGTCACTCAGGGCCTGT  >  NC_002947/1754418‑1754709                                                                                                                                                    |                                                                                                                                                  cTATCAGGACGGCTACTTCGATGCGGATGCGCCAGGTCTATGGATCGTTACTGCCAAGGGCCTGATGCGTAACGCCGAGGGCGGCAAGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAg                                                                                                                                                   <  1:366582/148‑1 (MQ=255)           ggCTACTTCGATGCGGATGCGCCAGGTCTATGGATCGTTACTGCCAAGGGCCTGATGCGTAACGCCGAGGGCGGCAAGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCt                                                                                                                                        >  3:350396/1‑149 (MQ=255)            gCTACTTCGATGCGGATGCGCCAGGTCTATGGATCGTTACTGCCAAGGGCCTGATGCGTAACGCCGAGGGCGGCAAGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTc                                                                                                                                       <  5:209275/149‑1 (MQ=255)            gCTACTTCGATGCGGATGCGCCAGGTCTATGGATCGTTACTGCCAAGGGCCTGATGCGTAACGCCGAGGGCGGCAAGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTc                                                                                                                                       <  7:145235/149‑1 (MQ=255)                cTTCGATGCGGATGCGCCAGGTCTATGGATCGTTACTGCCAAGGGCCTGATGCGTAACGCCGAGGGCGGCAAGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACg                                                                                                                                   >  4:296914/1‑149 (MQ=255)                cTTCGATGCGGATGCGCCAGGTCTATGGATCGTTACTGCCAAGGGCCTGATGCGTAACGCCGAGGGCGGCAAGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACg                                                                                                                                   >  2:39360/1‑149 (MQ=255)                 ttCGATGCGGATGCGCCAGGTCTATGGATCGTTACTGCCAAGGGCCTGATGCGTAACGCCGAGGGCGGCAAGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGt                                                                                                                                  >  6:300763/1‑149 (MQ=255)                    gatgcggatgcgCCAGGTCTATGGATCGTTACTGCCAAGGGCCTGATGCGTAACGCCGAGGGCGGCAAGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTc                                                                                                                                 >  5:366378/1‑147 (MQ=255)                        cggatgcgCCAGGTCTATGGATCGTTACTGCCAAGGGCCTGATGCGTAACGCCGAGGGCGGCAAGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCgagga                                                                                                                            <  8:331894/148‑1 (MQ=255)                            tgcgCCAGGTCTATGGATCGTTACTGCCAAGGGCCTGATGCGTAACGCCGAGGGCGGCAAGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGAttt                                                                                                                       <  7:133723/149‑1 (MQ=255)                              cgcCAGGTCTATGGATCGTTACTGCCAAGGGCCTGATGCGTAACGCCGAGGGCGGCAAGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTg                                                                                                                      <  6:99176/148‑1 (MQ=255)                               gcCAGGTCTATGGATCGTTACTGCCAAGGGCCTGATGCGTAACGCCGAGGGCGGCAAGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAACGCTCGACGTCGAGGAGATTTGt                                                                                                                     >  5:262422/1‑148 (MQ=255)                               gcCAGGTCTATGGATCGTTACTGCCAAGGGCCTGATGCGTAACGCCGAGGGCGGCAAGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGt                                                                                                                     >  5:39636/1‑148 (MQ=255)                               gcCAGGTCTATGGATCGTTACTGCCAAGGGCCTGATGCGTAACGCCGAGGGCGGCAAGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGt                                                                                                                     >  4:71876/1‑148 (MQ=255)                               gcCAGGTCTATGGATCGTTACTGCCAAGGGCCTGATGCGTAACGCCGAGGGCGGCAAGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTc                                                                                                                    <  1:39360/149‑1 (MQ=255)                                 cAGGTCTATGGATCGTTACTGCCAAGGGCCTGATGCGTAACGCCGAGGGCGGCAAGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCAc                                                                                                                  <  4:350396/149‑1 (MQ=255)                                         tGGATCGTTACTGCCAAGGGCCTGATGCGTAACGCCGAGGGCGGCAAGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTcc                                                                                                           <  3:296914/148‑1 (MQ=255)                                           gATCGTTACTGCCAAGGGCCTGATGCGTAACGCCGAGGGCGGCAAGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAg                                                                                                         <  5:300763/148‑1 (MQ=255)                                            aTCGTTACTGCCAAGGGCCTGATGCGTAACGCCGAGGGCGGCAAGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAggc                                                                                                       >  2:190650/1‑148 (MQ=255)                                            aTCGTTACTGCCAAGGGCCTGATGCGTAACGCCGAGGGCGGCAAGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGt                                                                                                       >  7:14807/1‑149 (MQ=255)                                                    tGCCAAGGGCCTGATGCGTAACGCCGAGGGCGGCAAGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTc                                                                                                            >  3:203512/1‑136 (MQ=255)                                                    tGCCAAGGGCCTGATGCGTAACGCCGAGGGCGGCAAGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTc                                                                                                            <  4:203512/136‑1 (MQ=255)                                                    tGCCAAGGGCCTGATGCGTAACGCCGAGGGCGGCAAGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCAt                                                                                               >  5:259607/1‑149 (MQ=255)                                                    tGCCAAGGGCCTGATGCGTAACGCCGAGGGCGGCAAGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAAGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCAt                                                                                               >  4:265514/1‑149 (MQ=255)                                                    tGCCAAGGGCCTGATGCGTAACGCCGAGGGCGGCAAGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAAGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCAt                                                                                               >  8:110287/1‑149 (MQ=255)                                                    tGCCAAGGGCCTGATGCGTAACGCCGAGGGCGGCAAGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAAGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCAt                                                                                               >  4:260611/1‑149 (MQ=255)                                                     gCCAAGGGCCTGATGCGTAACGCCGAGGGCGGCAAGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCATg                                                                                              >  5:334534/1‑149 (MQ=255)                                                     gCCAAGGGCCTGATGCGTAACGCCGAGGGCGGCAAGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCATg                                                                                              >  5:312164/1‑149 (MQ=255)                                                     gCCAAGGGCCTGATGCGTAACGCCGAGGGCGGCAAGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCATg                                                                                              >  5:307137/1‑149 (MQ=255)                                                       cAAGGGCCTGATGCGTAACGCCGAGGGCGGCAAGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCATGAg                                                                                            <  6:307137/149‑1 (MQ=255)                                                       cAAGGGCCTGATGCGTAACGCCGAGGGCGGCAAGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCATGAg                                                                                            <  6:312164/149‑1 (MQ=255)                                                       cAAGGGCCTGATGCGTAACGCCGAGGGCGGCAAGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCATGAg                                                                                            <  8:14807/149‑1 (MQ=255)                                                        aaGGGCCTGATGCGTAACGCCGAGGGCGGCAAGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCATGa                                                                                             >  6:221087/1‑147 (MQ=255)                                                        aaGGGCCTGATGCGTAACGCCGAGGGCGGCAAGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCATGa                                                                                             <  5:221087/147‑1 (MQ=255)                                                        aaGGGCCTGATGCGTAACGCCGAGGGCGGCAAGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCATGAg                                                                                            >  5:17450/1‑148 (MQ=255)                                                               tGATGCGTAACGCCGAGGGCGGCAAGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCATGAGGAAAAGc                                                                                     >  2:209724/1‑148 (MQ=255)                                                                gATGCGTAACGCCGAGGGCGGCAAGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCATGAGGAAAAGCCa                                                                                   >  1:293785/1‑149 (MQ=255)                                                                     gTAACGCCGAGGGCGGCAAGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCATGAGGAAAAGCCACGGcc                                                                              <  6:343905/149‑1 (MQ=255)                                                                     gTAACGCCGAGGGCGGCAAGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCATGAGGAAAAGCCACGGcc                                                                              >  5:343905/1‑149 (MQ=255)                                                                      tAACGCCGAGTGCTGCAAGGTGGTTAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCATGAGGAAAAGCCACGGccc                                                                             <  6:259607/149‑1 (MQ=255)                                                                         cGCCGAGGGCGGCAAGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCATGAGGAAAAGCCACGGCCCATc                                                                          <  7:15135/149‑1 (MQ=255)                                                                          gCCGAGGGCGGCAAGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCATGAGGAAAAGCCACGGCCCATcc                                                                         >  8:57947/1‑149 (MQ=255)                                                                             gAGGGCGGCAAGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCATGAGGAAAAGCCACGGCCCATCCtt                                                                       >  5:276481/1‑148 (MQ=255)                                                                                   ggcAAGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCATGAGGAAAAGCCACAGCCCATCCTTCaagaag                                                                <  6:366378/149‑1 (MQ=255)                                                                                      aaGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAAGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCATGAGGAAAAGCCACGGCCCATCCTTCAAGAAG‑Gcc                                                            <  7:110287/149‑1 (MQ=255)                                                                                      aaGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAAGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCATGAGGAAAAGCCACGGCCCATCCTTCAAGAAG‑Gcc                                                            <  3:260611/149‑1 (MQ=255)                                                                                      aaGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAAGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCATGAGGAAAAGCCACGGCCCATCCTTCAAGAAG‑Gcc                                                            <  3:265514/149‑1 (MQ=255)                                                                                         gtggtgAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCATGAGGAAAAGCCACGGCCCATCCTTCAAGAAG‑GCCGt                                                          >  6:178504/1‑148 (MQ=255)                                                                                            gtgAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCATGAGGAAAAGCCACGGCCCATCCTTCAAGAAG‑GCCGTGATc                                                      <  6:39636/149‑1 (MQ=255)                                                                                                   aCTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCATGAGGAAAAGCCACGGCCCATCCTTCAAGAAG‑GCCGTGATCGAGTTg                                                >  1:249050/1‑148 (MQ=255)                                                                                                   aCTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCATGAGGAAAAGCCACGGCCCATCCTTCAAGAAG‑GCCGTGATCGAGTTg                                                >  8:320772/1‑148 (MQ=255)                                                                                                       ggCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCATGAGGAAAAGCCACGGCCCATCCTTCAAGAAG‑GCCGTGATCGAGTTGGAt                                             <  5:178504/147‑1 (MQ=255)                                                                                                        gcaggCTGTGATCCGCAACATCTGCTCCCACTGCGTGGCGACGACGCGCCAAAGCTCCACGTCGAGCAGATTTGTCACCAGTTCCACGTGCCGGCATGAGGAAAAGCCACGGCCCATACTTCAAGAAT‑GCACTGATCGAGTTGGATGTa                                          >  4:92530/4‑149 (MQ=255)                                                                                                                tgATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCATGAGGAAAAGCCACGGCCCATCCTTCAAGAAGAGCCGTGATCGAGTTGGATGTATGCCCtt                                   <  1:209724/149‑1 (MQ=255)                                                                                                                      gCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCATGAGGAAAAGCCACGGCCCATCCTTCaag                                                                   >  1:19104/1‑111 (MQ=255)                                                                                                                      gCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCATGAGGAAAAGCCACGGCCCATCCTTCaag                                                                   <  2:19104/111‑1 (MQ=255)                                                                                                                      gCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCATGAGGAAAAGCCACGGCCCATCCTTCAAGAAG‑GCCGTGATCGAGtt                                                 <  8:273030/128‑1 (MQ=255)                                                                                                                      gCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCATGAGGAAAAGCCACGGCCCATCCTTCAAGAAG‑GCCGTGATCGAGtt                                                 >  7:273030/1‑128 (MQ=255)                                                                                                                         aCATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCATGAGGAAAAGCCACGGCCCATCCTTCAAGAAG‑GCCGTGATCGAGTTGGATGTATGCCCTTTGTGCCGTgg                         <  2:249050/149‑1 (MQ=255)                                                                                                                           aTCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCATGAGGAAAAGCCACGGCCCATCCTTCAAGAAG‑GCCGTGATCGAGTTGGATGTATGCCCTTTGTGCCGTgg                         >  2:66348/1‑147 (MQ=255)                                                                                                                           aTCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCATGAGGAAAAGCCACGGCCCATCCTTCAAGAAG‑GCCGTGATCGAGTTGGATGTATGCCCTTTGTGCCGTGGga                       <  7:320772/149‑1 (MQ=255)                                                                                                                             cTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCATGAGGAAAAGCCACGGCCCATCCTTCAAGAAG‑GCCGTGATCGAGTTGGATGTATGCCCTTTGTGCCGTGGga                       >  6:192116/1‑147 (MQ=255)                                                                                                                              tGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCATGAGGAAAAGCCACGGCCCATCCTTCAAGAAG‑GCCGTGATCGAGTTGGATGTATGCCCTTtgt                                <  1:102553/137‑1 (MQ=255)                                                                                                                              tGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCATGAGGAAAAGCCACGGCCCATCCTTCAAGAAG‑GCCGTGATCGAGTTGGATGTATGCCCTTtgt                                >  2:102553/1‑137 (MQ=255)                                                                                                                                ctcccACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCATGAGGAAAAGCCACGGCCCATCCTTCAAGAAG‑GCCGTGATCGAGTTGGATGTATGCCCTTTGTGCCGTGGgaga                     <  3:92530/144‑1 (MQ=255)                                                                                                                                cgcCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCATGAGGAAAAGCCACGGCCCATCCTTCAAGAAG‑GTCGTGATCGAGTTGGATGTATGCCCTTTGTGCCGTGGGagag                    <  4:272788/147‑1 (MQ=255)                                                                                                                                 gcCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCg                                                                                                   <  7:285777/68‑1 (MQ=255)                                                                                                                                 gcCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCg                                                                                                   >  8:285777/1‑68 (MQ=255)                                                                                                                                  cccACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCATGAGGAAAAGCCACGGCCCATCCTTCAAGAAG‑GCCGTGATCGAGTTGGATGTATGCCCTTTGTGCCGTGGGAGAGCGGt                <  6:334534/149‑1 (MQ=255)                                                                                                                                  cccACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCATGAGGAAAAGCCACGGCCCATCCTTCAAGAAG‑GCCGTGATCGAGTTGGATGTATGCCCTTTGTGCCGTGGGAGAGCGGt                <  3:71876/149‑1 (MQ=255)                                                                                                                                               gaggaGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCATGAGGAAAAGCCACGGCCCATCCTTCAAGAAG‑GCCGTGATCGAGTTGGATGTATGCCCTTTGTGCCGTGGGAGAGCGGTCACTCAgg        <  1:313377/141‑1 (MQ=255)                                                                                                                                               gacgaGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCATGAGGAAAAGCCACGGCCCATCCTTCAAGAAG‑GCCGTGATCGAGTTGGATGTATGCCCTTTGTGCCGTGGGAGAGCGGTCACTCAgg        >  2:313377/1‑144 (MQ=255)                                                                                                                                                acgaGGCTCCAAAGCTCGACGGCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCATGAGGAAAAGCCACGGCCCATCCTTCAAGAAG‑GCCGTGATCGAGTTGGATGTATGCCCTTTGTGCCGTGGGAGAGCGGTCACTCAGGGCCTGt  <  6:262422/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                  CTATCAGGACGGCTACTTCGATGCGGATGCGCCAGGTCTATGGATCGTTACTGCCAAGGGCCTGATGCGTAACGCCGAGGGCGGCAAGGTGGTGAGCAACTTGGCCGGCTGTGATCCGCAACATCTGCGCCCACTGCGTGGCGACGAGGCGCCAAAGCTCGACGTCGAGGAGATTTGTCACCTGTTCCAGGTGCCGGCATGAGGAAAAGCCACGGCCCATCCTTCAAGAAG‑GCCGTGATCGAGTTGGATGTATGCCCTTTGTGCCGTGGGAGAGCGGTCACTCAGGGCCTGT  >  NC_002947/1754418‑1754709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑