Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,780,086	T→A	26.8%	intergenic (+45/+61)	PP_1587 → / ← dapC	NhaP‑type Na+(K+)/H+ antiporter/N‑succinyl‑L,L‑diaminopimelate aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,780,086	0	T	A	26.8%	19.1 / 5.9	15	intergenic (+45/+61)	PP_1587/dapC	NhaP‑type Na+(K+)/H+ antiporter/N‑succinyl‑L,L‑diaminopimelate aminotransferase
Reads supporting (aligned to +/- strand):  ref base T (4/7);  new base A (2/2);  total (6/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-01

TACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTA‑TCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATGCG  >  NC_002947/1779943‑1780224                                                                                                                                                 |                                                                                                                                           tACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTA‑TCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTActgcgct                                                                                                                                       <  8:97944/149‑1 (MQ=255)    aaCCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTA‑TCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTActgcgctgcg                                                                                                                                    <  2:1335/149‑1 (MQ=255)            tCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTA‑TCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACtgcgctgcgc                                                                                                                                   <  5:88651/142‑1 (MQ=255)            tCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTA‑TCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACtgcgctgcgc                                                                                                                                   >  6:88651/1‑142 (MQ=255)              gTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTACTCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAAt                                                                                                                            <  1:101393/148‑1 (MQ=255)                     ccTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTA‑TCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGt                                                                                                                   <  7:190246/149‑1 (MQ=255)                             ggtaTGAGGTGGTTCGGCAAGTACTGGGTGAGCTGGATATGTGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTA‑TCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACAGCGCTGATTTCAAGCGCAGCGCAGTACCTGTgg                                                                                                           <  7:175873/146‑1 (MQ=21)                             gggATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTA‑TCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTgg                                                                                                           <  7:284984/149‑1 (MQ=255)                               gATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTA‑TCAAGGCCCTTCGCGGGCACGCCCGCTCCCACAGGTACAGCGCTGATTTCAAGCGCAGCGCAGTACCTGTGGGa                                                                                                         <  5:308984/149‑1 (MQ=21)                                     gtggggCGGCTAGTAGTGTGTGAGGTGCATATGAGCTAGGCCATCCTGGGTTCTGTGAAGTAGACCCTA‑TCCAGGCACTTCGCGCGCGGGCCCGCTCCCACTCGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGc                                                                                                   <  6:95648/149‑1 (MQ=255)                                                                     gagtgagGCGAACCTGGGTTCAGTCAAGTAGACCCTA‑TCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAgcg                                                                                                                       >  8:119422/1‑97 (MQ=255)                                                                     gagtgagGCGAACCTGGGTTCAGTCAAGTAGACCCTA‑TCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAgcg                                                                                                                       <  7:119422/97‑1 (MQ=255)                                                                       gtgagGCGAACCTGGGTTCAGTCAAGTAGACCCTA‑TCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTAc                                                                                                                 <  1:361195/101‑1 (MQ=255)                                                                       gtgagGCGAACCTGGGTTCAGTCAAGTAGACCCTA‑TCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTAc                                                                                                                 >  2:361195/1‑101 (MQ=255)                                                                                                                                  cGCTCCCACAGGTACAGCGCTGATTTCAAGCGCAGCGCAGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGc        >  2:55396/1‑148 (MQ=21)                                                                                                                                      cccACAGGTACAGCGCTGATTTCAAGCGCAGCGCAGTACCTGTGGGAGCGGGCGCGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATgc   >  3:108960/1‑149 (MQ=21)                                                                                                                                       ccACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATgcg  >  8:143354/1‑149 (MQ=255)                                                                                                                                        cacaGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATgc                                                   >  6:63036/1‑99 (MQ=255)                                                                                                                                        cacaGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATgc                                                   <  5:63036/99‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                           TACAACCTGCATCGTCAGCACCTGGTGGGGGATGAGGTGGTGCGGCAAGTACTGGGTGAGCTGGATATGAGTGAGGCGAACCTGGGTTCAGTCAAGTAGACCCTA‑TCAAGGCCCTTCGCGGGCGCGCCCGCTCCCACAGGTACTGCGCTGCGCTTGAAATCAGCGCTGTACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGTTGATCAGCGGTTTTGCAGGAAGGCGCGAATGCGCTCCGCCGCTTCGATGCATTCGGCCAGCGGTGCAACCAGTGCCATGCG  >  NC_002947/1779943‑1780224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑