Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,787,066	C→G	8.1%	intergenic (+33/‑149)	tsf → / → pyrH	elongation factor Ts/uridylate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,787,066	0	C	G	8.1%	194.1 / 6.7	62	intergenic (+33/‑149)	tsf/pyrH	elongation factor Ts/uridylate kinase
Reads supporting (aligned to +/- strand):  ref base C (28/29);  new base G (3/2);  total (31/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.93e-01

AGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGT‑TGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGTTTATACGACTCCCTCTTCGCTGGCACCGAAGCGGTGCCACGCTACAGTTAGCAGGCTGCAAACAGCCCGCACGAATTTTCTAAAAGTACGCCGCAGGAGA  >  NC_002947/1786919‑1787206                                                                                                                                                     |                                                                                                                                             aGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGT‑TGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGccc                                                                                                                                             >  2:329957/1‑149 (MQ=255)   ccGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGT‑TGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGc                                                                                                                                           <  1:329957/149‑1 (MQ=255)          aGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGT‑TGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCAcgcg                                                                                                                                    >  4:19968/1‑149 (MQ=255)          aGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGT‑TGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCAcgcg                                                                                                                                    >  3:268906/1‑149 (MQ=255)             aaTCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGT‑TGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCAcgcgcg                                                                                                                                  >  3:169453/1‑148 (MQ=255)              aTCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGT‑TGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCa                                                                                                                                <  7:185671/149‑1 (MQ=255)                      cTTCACCTACTTCAACGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGT‑TGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCtt                                                                                                                         <  7:308555/148‑1 (MQ=255)                      cTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGT‑TGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCGCGCGTGAGCGGGCAGCctc                                                                                                                           >  4:355454/1‑146 (MQ=255)                      cTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGT‑TGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCGCGCGTGAGCGGGCAGCctc                                                                                                                           <  3:355454/146‑1 (MQ=255)                      cTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGT‑TGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCttt                                                                                                                        <  4:268906/149‑1 (MQ=255)                      cTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGT‑TGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCttt                                                                                                                        <  2:65645/149‑1 (MQ=255)                      cTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGT‑TGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCttt                                                                                                                        <  8:108929/149‑1 (MQ=255)                           ccTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGT‑TGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCctct                                                                                                                          <  8:160102/142‑1 (MQ=255)                           ccTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGT‑TGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCctct                                                                                                                          >  7:160102/1‑142 (MQ=255)                            cTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGTATGCCGATCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCa                                                                                                                    >  1:106652/1‑148 (MQ=255)                            cTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGT‑TGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCaaa                                                                                                                  <  3:340949/149‑1 (MQ=255)                               cTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGT‑TGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACg                                                                                                               >  7:318268/1‑149 (MQ=255)                                ttCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGT‑TGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACgg                                                                                                              >  8:187548/1‑149 (MQ=255)                                ttCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGT‑TGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACgg                                                                                                              >  4:172751/1‑149 (MQ=255)                                         ggCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGT‑TGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGtt                                                                                                     >  7:281924/1‑149 (MQ=255)                                          gCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGT‑TGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGttt                                                                                                    >  5:296201/1‑149 (MQ=255)                                           cGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGT‑TGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGTTta                                                                                                   <  3:19968/149‑1 (MQ=255)                                            gAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGT‑TGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGTTtat                                                                                                  <  1:339020/149‑1 (MQ=255)                                            gAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGT‑TGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGTTta                                                                                                   >  1:214294/1‑148 (MQ=255)                                            gAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGT‑TGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGTTta                                                                                                   >  1:97685/1‑148 (MQ=255)                                             aaGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGT‑TGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGTTtata                                                                                                 <  2:106652/149‑1 (MQ=255)                                                     gagaAGCCAGTCGACGACTTCGCTGCTGAAGT‑TGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGTTTATACGACTcc                                                                                          <  2:214294/148‑1 (MQ=255)                                                       gaAGCCAGTCGACGACTTCGCTGCTGAAGT‑TGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGTTTATACGACTCCctc                                                                                       >  2:1258/1‑149 (MQ=255)                                                       gaAGCCAGTCGACGACTTCGCTGCTGAAGT‑TGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGTTTATACGACTCCctc                                                                                       <  8:281924/149‑1 (MQ=255)                                                       gaAGCCAGTCGACGACTTCGCTGCTGAAGT‑TGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGTTTATACGACTCCctc                                                                                       >  2:6923/1‑149 (MQ=255)                                                           ccAGTCGACGACTTCGCTGCTGAAGT‑TGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGTTTATACGACTCCCTCTTCg                                                                                   >  1:93908/1‑149 (MQ=255)                                                             aGTCGACGACTTCGCTGCTGAAGT‑TGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGTTTATACGACTCCCTCTTCGCt                                                                                 >  5:328311/1‑149 (MQ=255)                                                                       ttCGCTGCTGAAGT‑TGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGTTTATACGACTCCCTCTTCGCTGGCACCGAAg                                                                       >  5:121465/1‑149 (MQ=255)                                                                          gctgctGAAGT‑TGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGTTTATACGACTCCCTCTTCGCTGGCACCGAAGCg                                                                     <  1:189136/148‑1 (MQ=255)                                                                            tgctgAAGT‑TGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGTTTATACGACTCCCTCTTc                                                                                    <  4:199319/131‑1 (MQ=255)                                                                            tgctgAAGT‑TGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGTTTATACGACTCCCTCTTc                                                                                    >  3:199319/1‑131 (MQ=255)                                                                            tgctgAAGT‑TGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGTTTATACGACTCCCTCTTCGCTGGCACCGAAGCGGTg                                                                  <  8:318268/149‑1 (MQ=255)                                                                            tgctgAAGT‑TGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGTTTATACGACTCCCTCTTCGCTGGCACCGAAGCGGTg                                                                  >  4:315018/1‑149 (MQ=255)                                                                                          gCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGTTTATACGACTCCCTCTTCGCTGGCACCGAAGCGGTGCCACGCTACAGtt                                                     <  6:328311/149‑1 (MQ=255)                                                                                          gCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGTTTATACGACTCCCTCTTCGCTGGCACCGAAGCGGTGCCACGCTACAGt                                                      >  5:80102/1‑148 (MQ=255)                                                                                                gTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGTTTATACGACTCCCTCTTCGCTGGCACCGAAGCGGTGCCACGCTACAGtt                                                     >  1:253923/1‑143 (MQ=255)                                                                                                gTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGTTTATACGACTCCCTCTTCGCTGGCACCGAAGCGGTGCCACGCTACAGtt                                                     <  2:253923/143‑1 (MQ=255)                                                                                                   gctgctGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGTTTATACGACTCCCTCTTCGCTGGCACCGAAGCGGTGCCACGCTACAGTTAGCAGGCTg                                            >  2:246468/1‑149 (MQ=255)                                                                                                     tgctgcCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGTTTATACGACTCCCTCTTCGCTGGCACCGAAGCGGTGCCACGCTACAGTTAGCAGGCTGc                                           >  6:50280/1‑148 (MQ=255)                                                                                                           cAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGATGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGTTTATACGACTCCCTCTTCGCTGGCACCGAAGCGGTGCCACGCTACAGTTAGCAGGCTGCAAACAGc                                    <  8:16867/149‑1 (MQ=255)                                                                                                            aaGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCGCGCGTGAGCGGGCAGCctct                                                                                                                          >  1:296229/1‑62 (MQ=17)                                                                                                            aaGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCGCGCGTGAGCGGGCAGCctc                                                                                                                           <  2:296229/61‑1 (MQ=17)                                                                                                             aGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGTTTATACGACTCCCTCTTCGCTGGCACCGAAGCGGTGCCACGCTACAGTTAGCAGGCTGCAAACAGccc                                  <  2:97685/149‑1 (MQ=255)                                                                                                             aGCAGTAAGACAGCCCCGTCTGTCGCACCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGTTTATACGACTCCCTCTTCGCTGGCACCGAAGCGGTGCCACGCTACAGTTAGCAGGCTGCAAACAGccc                                  >  3:226518/1‑149 (MQ=255)                                                                                                              gCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGTTTATACGACTCCCTCTTCGCTGGCACCGAAGCGGTGCCACGCTACAGTTAGCAGGCTGCAAACAGCCCg                                 <  5:50280/149‑1 (MQ=255)                                                                                                                    aGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGTTTATACGACTCCCTCTTCGCTGGCACCGAAGCGGTGCCACGCTACAGTTAGCAGGCTGCAAACAGCCCGCACGaa                           >  3:295628/1‑149 (MQ=255)                                                                                                                      aCAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGTTTATACGACTCCCTCTTCGCTGGCACCGAAGCGGTGCCACGCTACAGTTAGCAGGCTGCAAACAGCCCGCACGAAtt                         >  1:192701/1‑149 (MQ=255)                                                                                                                            ccGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGTTTATACGACTCCCTCTTCGCTGGCACCGAAGCGGTGCCACGCTACAGTTAGCAGGCTGCAAACAGCCCGCACGAATTTTCTaa                   <  1:6923/149‑1 (MQ=255)                                                                                                                            ccGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGTTTATACGACTCCCTCTTCGCTGGCACCGAAGCGGTGCCACGCTACAGTTAGCAGGCTGCAAACAGCCCGCACGAATTTTCTaa                   <  1:1258/149‑1 (MQ=255)                                                                                                                              gTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGTTTATACGACTCCCTCTTCGCTGGCACCGAAGCGGTGCCACGCTACAGTTAGCAGGCTGCAAACAGCCCGCACGAATTTTCTaaaa                 <  6:121465/149‑1 (MQ=255)                                                                                                                              gTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGTTTATACGACTCCCTCTTCGCTGGCACCGAAGCGGTGCCACGCTACAGTTAGCAGGCTGCAAACAGCCCGCACGAATTTTCTaaaa                 <  7:187548/149‑1 (MQ=255)                                                                                                                                     gCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGTTTATACGACTCCCTCTTCGCTGGCACCGAAGCGGTGCCACGCTACAGTTAGCAGGCTGCAAACAGCCCGCACGAATTTTCTAAAAGTAcgcc          <  4:169453/149‑1 (MQ=255)                                                                                                                                         cAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGTTTATACGACTCCCTCTTCGCTGGCACCGAAGCGGTGCCACGCTACAGTTAGCAGGCTGCAAACAGCCCGCACGAATTTTCTAAAAGTACGCCGCAg      >  5:119824/1‑149 (MQ=255)                                                                                                                                           aaGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGTTTATACGACTCCCTCTTCGCTGGCACCGAAGCGGTGCCACGCTACAGTTAGCAGGCTGCAAACAGCCCGCACGAATTTTCTAAAAGTACGCCGCAg      >  3:361708/1‑147 (MQ=255)                                                                                                                                           aaGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGTTTATACGACTCCCTCTTCGCTGGCACCGAAGCGGTGCCACGCTACAGTTAGCAGGCTGCAAACAGCCCGCACGAATTTTCTAAAAGTACGCCGCAGga    <  4:295628/149‑1 (MQ=255)                                                                                                                                            agagGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGTTTATACGACTCCCTCTTCGCTGGCACCGAAGCGGTGCCACGCTACAGTTAGCAGGCTGCAAACAGCCCGCACGAATTTTCTAAAAGTACGCCGCAGgag   <  6:119824/149‑1 (MQ=255)                                                                                                                                             gagGCTGCGCGCGTGAGCGGGCAGCCTCTTTGTCAAAACGGGGAGGAGTTTATACGACTCCCTCTTCGCTGGCACCGAAGCGGTGCCACGCTACAGTTAGCAGGCTGCAAACAGCCCGCACGAATTTTCTAAAAGTACGCCGCAGga    >  7:137153/1‑147 (MQ=255)                                                                                                                                             gagGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGTTTATACGACTCCCTCTTCGCTGGCACCGAAGCGGTGCCACGCTACAGTTAGCAGGCTGCAAACAGCCCGCACGAATTTTCTAAAAGTACGCCGCAGgaga  <  2:192701/149‑1 (MQ=255)                                                                                                                                                     ccGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGTTTATACGACTCCCTCTTCGCTGGCACCGAAGCGGTGCCACGCTACAGTTAGCAGGCTGCAAACAGCCCGCACGAATTTTCTAAAAGTACGCCGCAGgaga  <  7:64871/141‑1 (MQ=255)                                                                                                                                                     ccGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGTTTATACGACTCCCTCTTCGCTGGCACCGAAGCGGTGCCACGCTACAGTTAGCAGGCTGCAAACAGCCCGCACGAATTTTCTAAAAGTACGCCGCAGgaga  >  8:64871/1‑141 (MQ=255)                                                                                                                                                     |                                                                                                                                             AGCCGGTGCAGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCATCGAGAAGCCAGTCGACGACTTCGCTGCTGAAGT‑TGCCGCTCAGGTCGCTGCTGCCAAGCAGTAAGACAGCCCCGTCTGTCGCCCCAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTGTCAAAACGGGGAGGAGTTTATACGACTCCCTCTTCGCTGGCACCGAAGCGGTGCCACGCTACAGTTAGCAGGCTGCAAACAGCCCGCACGAATTTTCTAAAAGTACGCCGCAGGAGA  >  NC_002947/1786919‑1787206

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑