Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,846,286	T→A	5.1%	W193R (TGG→AGG)	pfeS‑II →	histidine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,846,286	0	T	A	5.1%	239.9 / 2.5	79	W193R (TGG→AGG)	pfeS‑II	histidine kinase
Reads supporting (aligned to +/- strand):  ref base T (39/36);  new base A (2/2);  total (41/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.96e-01

GCAGCGCTTCAATCCGGGCCAGCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGC‑TGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTTGCTGCGCGACCTGTC  >  NC_002947/1846138‑1846434                                                                                                                                                      |                                                                                                                                                      gCAGCGCTTCAATCCGGGCCAGCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGC‑TGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCt                                                                                                                                                       >  2:224846/1‑149 (MQ=255) gCAGCGCTTCAATCCGGGCCAGCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGC‑TGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCt                                                                                                                                                       >  2:219496/1‑149 (MQ=255) gCAGCGCTTCAATCCGGGCCAGCACCGCTTGCTCTGGCGCATTGTCACCAACGGCATCATCCCGGGGC‑TGTTCACCTAGCTGTATTGTGTAGGCCTGTACCGCATGCTGATCGTGCGGCTGACCCAACTTCGCGAGCAGGCCAATGCCt                                                                                                                                                       >  8:175594/1‑149 (MQ=255)    gcgcTTCAATCCGGGCCAGCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGC‑TGTTCACCTTGCTGTTGTGTGTAGGCTTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGc                                                                                                                                                    >  4:204415/1‑149 (MQ=255)    gcgcTTCAATCCGGGCCAGCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGC‑TGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGc                                                                                                                                                    <  1:219496/149‑1 (MQ=255)    gcgcTTCAATCCGGGCCAGCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGC‑TGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGc                                                                                                                                                    <  1:224846/149‑1 (MQ=255)        ttCAATCCGGGCCAGCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGC‑TGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGc                                                                                                                                                >  1:275886/1‑149 (MQ=255)           aaTCCGGGCCAGCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGC‑TGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGcc                                                                                                                                               >  5:137794/1‑147 (MQ=255)           aaTCCGGGCCAGCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGC‑TGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGcc                                                                                                                                               <  6:137794/147‑1 (MQ=255)                      gCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGC‑TGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCgg                                                                                                                                 >  6:76822/1‑149 (MQ=255)                         ccGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGC‑TGTTCACCTTGCTGTTGTGTGTAGGCTTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGccc                                                                                                                              <  3:204415/149‑1 (MQ=255)                          cGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGC‑TGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCg                                                                                                                             >  8:128827/1‑149 (MQ=255)                                 tgtgGCGCATTGTCACCAACGGCATCATCCCGGGGC‑TGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGAc                                                                                                                      >  3:183776/1‑149 (MQ=255)                                 tgtgGCGCATTGTCACCAACGGCATCATCCCGGGGC‑TGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGAc                                                                                                                      >  7:168122/1‑149 (MQ=255)                                   tgGCGCATTGTCACCAACGGCATCATCCCGGGGC‑TGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTc                                                                                                                    <  5:76822/149‑1 (MQ=255)                                     gcgcATTGTCACCAACGGCATCATCCCGGGGC‑TGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTcgc                                                                                                                  <  8:168122/149‑1 (MQ=255)                                     gcgcATTGTCACCAACGGCATCATCCCGGGGC‑TGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTcgc                                                                                                                  <  7:128827/149‑1 (MQ=255)                                         aTTGTCACCAACGGCATCATCCCGGGGCATGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCa                                                                                                               >  1:22388/1‑149 (MQ=255)                                             tCACCAACGGCATCATCCCGGGGC‑TGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTg                                                                                                                         <  4:194885/134‑1 (MQ=255)                                             tCACCAACGGCATCATCCCGGGGC‑TGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTg                                                                                                                         >  3:194885/1‑134 (MQ=255)                                             tCACCAACGGCATCATCCCGGGGC‑TGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCa                                                                                                               >  7:228344/1‑144 (MQ=255)                                             tCACCAACGGCATCATCCCGGGGC‑TGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCa                                                                                                               <  8:228344/144‑1 (MQ=255)                                             tCACCAACGGCATCATCCCGGGGC‑TGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATc                                                                                                          >  4:83928/1‑149 (MQ=255)                                               aCCAACGGCATCATCCCGGGGC‑TGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGc                                                                                                        >  8:176755/1‑149 (MQ=255)                                                 cAACGGCATCATCCCGGGGC‑TGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGccc                                                                                                      >  3:53550/1‑149 (MQ=255)                                                  aaCGGCATCATCCCGGGGC‑TGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCAgg                                                                                                                                                     >  6:123838/1‑102 (MQ=255)                                                  aaCGGCATCATCCCGGGGC‑TGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCAgg                                                                                                                                                     <  5:123838/102‑1 (MQ=255)                                                       catcatCCCGGGGC‑TGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCAt                                                                                                 >  5:89575/1‑148 (MQ=255)                                                        atcatcCCGGGGC‑TGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGa                                                                                               <  4:53550/149‑1 (MQ=255)                                                         tcatcCCGGGGC‑TGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGAc                                                                                              <  4:139952/149‑1 (MQ=255)                                                         tcatcCCGGGGC‑GGTTCACGTTGCGCTTCTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGAc                                                                                              <  4:134379/149‑1 (MQ=255)                                                          catcCCGGGGC‑TGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGAc                                                                                              >  1:51786/1‑148 (MQ=255)                                                              ccGGGGC‑TGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAAt                                                                                          <  2:275886/148‑1 (MQ=255)                                                                ggggC‑TGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTgg                                                                                       >  2:197447/1‑149 (MQ=255)                                                                       gTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCAGGCGGGCCGA‑CAGCTGATCGGCCCGc                                                                                                                            >  3:274782/1‑106 (MQ=38)                                                                       gTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCAGGCGGGCCGA‑CAGCTGATCGGCCCGc                                                                                                                            <  4:274782/106‑1 (MQ=38)                                                                             cTTGCTTTTGTGTGTAGGCCTGTAGCGAATGCTGATCGTGCCGCTGAACCAACTTCGCTAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCGCCATCGCCCTGCATGACGAATTGGGCGAACTGgc                                                                             <  2:233735/147‑1 (MQ=255)                                                                             cTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCGGGCATGACGAATTGGGCGAACt                                                                                <  7:57455/144‑1 (MQ=255)                                                                             cTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACt                                                                                >  8:57455/1‑144 (MQ=255)                                                                             cTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTACGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCCCGCGCCATCGCCAGGCATGACGAATTGGGCGAACTGgc                                                                             >  1:233735/1‑147 (MQ=255)                                                                               tGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGt                                                                         >  5:98362/1‑149 (MQ=255)                                                                                  tgttgtGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTg                                                                        >  6:28477/1‑147 (MQ=255)                                                                                  tgttgtGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTg                                                                        <  5:28477/147‑1 (MQ=255)                                                                                  tgttgtGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGc                                                                       >  6:314025/1‑148 (MQ=255)                                                                                   gttgtGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGTAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCt                                                                     >  6:373755/1‑149 (MQ=255)                                                                                    ttgtGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGc                                                                       <  5:314025/146‑1 (MQ=255)                                                                                      gtgtgtAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGTAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCttt                                                                   <  5:373755/148‑1 (MQ=255)                                                                                       tgtgtAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGa                                                                 >  4:71027/1‑149 (MQ=255)                                                                                         tgtAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGgcg                                                                            >  1:87743/1‑136 (MQ=255)                                                                                         tgtAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGgcg                                                                            <  2:87743/136‑1 (MQ=255)                                                                                         tgtAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGAc                                                                <  1:197447/148‑1 (MQ=255)                                                                                           tAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTgg                                                                                       >  5:310860/1‑123 (MQ=255)                                                                                           tAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTgg                                                                                       <  6:310860/123‑1 (MQ=255)                                                                                           tAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCa                                                              <  3:83928/148‑1 (MQ=255)                                                                                                cTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑CCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGc                                                        >  2:59283/1‑149 (MQ=255)                                                                                                cTGAACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGc                                                        <  4:310601/149‑1 (MQ=255)                                                                                                  gTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATg                                                          >  6:109258/1‑145 (MQ=255)                                                                                                  gTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATg                                                          <  5:109258/145‑1 (MQ=255)                                                                                                     ccGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGc                                                   <  3:71027/149‑1 (MQ=255)                                                                                                         aTGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCt                                               <  2:22388/149‑1 (MQ=255)                                                                                                           gCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTg                                              >  6:322988/1‑148 (MQ=255)                                                                                                             tGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGACGAGAGGATGc                                             >  4:209577/1‑147 (MQ=255)                                                                                                              gATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAg                                          >  5:88601/1‑149 (MQ=255)                                                                                                               aTCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCaa                                           <  4:183776/147‑1 (MQ=255)                                                                                                               aTCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCaa                                           <  7:176755/147‑1 (MQ=255)                                                                                                                   tGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑CCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACg                                     <  1:59283/149‑1 (MQ=255)                                                                                                                      cGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTg                                  <  1:238616/149‑1 (MQ=255)                                                                                                                        cTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCAc                                      >  5:270828/1‑143 (MQ=255)                                                                                                                        cTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCAc                                      <  6:270828/143‑1 (MQ=255)                                                                                                                          gAACCAACTTCGCGAGCAGGCCAATGCCTCGCGGGCCGATCCGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTa                              >  5:186450/1‑149 (MQ=255)                                                                                                                           aaCCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTAt                             >  4:265949/1‑149 (MQ=255)                                                                                                                             ccAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGAc                                                                <  2:145183/112‑1 (MQ=255)                                                                                                                             ccAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGAc                                                                >  1:145183/1‑112 (MQ=255)                                                                                                                             ccAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATg                            >  2:347186/1‑148 (MQ=255)                                                                                                                               aaCTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGCCGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATgcag                         >  6:281485/1‑149 (MQ=255)                                                                                                                                  ttCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATg                            <  7:18531/143‑1 (MQ=255)                                                                                                                                  ttCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATg                            >  8:18531/1‑143 (MQ=255)                                                                                                                                         gCAGGCCAATGCCTGGCGGGCCGATCCGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTtgc               >  4:299968/1‑149 (MQ=255)                                                                                                                                          cAGGCCAATGCCTGGCGGGCCGATCCGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTtgct              <  3:299968/149‑1 (MQ=255)                                                                                                                                           aGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTtgctg             <  5:322988/149‑1 (MQ=255)                                                                                                                                             gCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCGGGACTCGCGCACCAACGCCGGGCATGACGAATTGGGCGAACTGGCGCGTGCATTTGACCAGATGGCCGAGCGGCGGCAAGGCACGGTGGCTATGCAGCAACtgctgctgca           <  7:175594/149‑2 (MQ=255)                                                                                                                                                   gCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCCGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGCCTCCAAGGCACGATTGCTATGCAGCAGCAGTTGCATCGCGACCt     >  8:346779/1‑149 (MQ=255)                                                                                                                                                      tGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTTGCTGCGCGACCTGTc  >  4:11269/1‑149 (MQ=255)                                                                                                                                                      |                                                                                                                                                      GCAGCGCTTCAATCCGGGCCAGCACCGCTTGCTGTGGCGCATTGTCACCAACGGCATCATCCCGGGGC‑TGTTCACCTTGCTGTTGTGTGTAGGCCTGTACCGCATGCTGATCGTGCCGCTGAACCAACTTCGCGAGCAGGCCAATGCCTGGCGGGCCGATCAGCTG‑TCGGCCCGCCTGGACTCGCGCACCATCGCCCGGCATGACGAATTGGGCGAACTGGCGCGTGCCTTTGACCAGATGGCCGAGCGGCTGCAAGGCACGGTGGCTATGCAGCAGCAGTTGCTGCGCGACCTGTC  >  NC_002947/1846138‑1846434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑