Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,859,108 C→G 7.6% intergenic (+28/+73) PP_1661 → / ← PP_1662 dehydrogenase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,859,1080CG 7.6% 206.5 / 5.5 65intergenic (+28/+73)PP_1661/PP_1662dehydrogenase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (34/26);  new base G (3/2);  total (37/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCAAGGTGAGTTGCAGCTGTGCATCAATGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTCG  >  NC_002947/1858968‑1859247
                                                                                                                                            |                                                                                                                                           
aGCAAGGTGAGTTGCAGCTGTGCATCAATGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATg                                                                                                                                     >  5:239875/1‑149 (MQ=255)
  cAAGGTGAGTTGCAGCTGTGCATCAATGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGcc                                                                                                                                   >  3:7196/1‑149 (MQ=255)
         aGTTGCAGCTGTGCATCAATGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCAAAATGCTGCGGCATTGCCGGAGCAt                                                                                                                            >  5:51401/1‑149 (MQ=255)
           ttGCAGCTGTGCATCAATGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCAttt                                                                                                                          <  1:214081/149‑1 (MQ=255)
           ttGCAGCTGTGCATCAATGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCAttt                                                                                                                          >  7:124073/1‑149 (MQ=255)
           ttGCAGCTGTGCATCAATGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCAtt                                                                                                                           <  6:239875/148‑1 (MQ=255)
               aGCTGTGCATCAATGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTggg                                                                                                                      <  6:239518/149‑1 (MQ=255)
                 cTGTGCATCAATGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGTTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGt                                                                                                                    <  4:7196/149‑1 (MQ=255)
                    tgCATCAATGACGACCTGGACGGGCGTTACTGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGAAGAGGTGCGTTTCGGGGCTTTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCTGCAATGCCGCAGCATTTTGGGGTTg                                                                                                                  <  2:228113/148‑1 (MQ=255)
                     gCATCAATGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGc                                                                                                                 >  4:285158/1‑148 (MQ=255)
                      cATCAATGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCAAAATGCTGCGGCATTGCCGGAGCATCGTGGGGTTGcca                                                                                                               <  6:51401/149‑3 (MQ=37)
                        tCAATGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTgggg                                                                                                                     >  8:89209/1‑141 (MQ=255)
                        tCAATGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTgggg                                                                                                                     <  7:89209/141‑1 (MQ=255)
                                   cTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGc                                                                                                  >  4:325220/1‑149 (MQ=255)
                                           gCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAgg                                                                                          <  8:124073/149‑1 (MQ=255)
                                              ttACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGt                                                                                       >  5:197624/1‑149 (MQ=255)
                                                   gggCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTcgcc                                                                                  >  4:940/1‑149 (MQ=255)
                                                   gggCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTcgcc                                                                                  >  4:6224/1‑149 (MQ=255)
                                                                  aTAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGa                                                                                                >  3:333918/1‑120 (MQ=255)
                                                                  aTAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGa                                                                                                <  4:333918/120‑1 (MQ=255)
                                                                     aCCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAg                                                                                                    >  6:312985/1‑113 (MQ=255)
                                                                     aCCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAg                                                                                                    <  5:312985/113‑1 (MQ=255)
                                                                     aCCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGc                                                                                                  <  7:66434/115‑1 (MQ=255)
                                                                     aCCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGc                                                                                                  >  8:66434/1‑115 (MQ=255)
                                                                      ccTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATtg                                                               >  6:303852/1‑149 (MQ=255)
                                                                      ccTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATtg                                                               >  4:18393/1‑149 (MQ=255)
                                                                      ccTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATtg                                                               >  8:17093/1‑149 (MQ=255)
                                                                       cTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATtgt                                                              >  1:110214/1‑149 (MQ=255)
                                                                       cTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATtgt                                                              >  2:154801/1‑149 (MQ=255)
                                                                            cAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGgc                                                         <  7:17093/149‑1 (MQ=255)
                                                                                         gagGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTc                                             <  3:940/148‑1 (MQ=255)
                                                                                         gagGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTc                                             <  3:6224/148‑1 (MQ=255)
                                                                                         gagGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTc                                             <  3:285158/148‑1 (MQ=255)
                                                                                          agGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCa                                           <  1:154801/149‑1 (MQ=255)
                                                                                           ggTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAg                                          >  2:229068/1‑149 (MQ=255)
                                                                                            gggCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAgg                                         <  5:363450/147‑1 (MQ=255)
                                                                                                 tttCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCtt                                     >  6:41297/1‑148 (MQ=255)
                                                                                                    ctgggCTGTGTAGGCCCTGTCATGGCAAGCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCaa                                  <  2:110214/146‑1 (MQ=255)
                                                                                                      gggCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCt                               >  2:150977/1‑149 (MQ=255)
                                                                                                             gtAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAAcgcg                        <  5:303852/149‑1 (MQ=255)
                                                                                                                 gCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTgg                                                          <  5:84730/111‑1 (MQ=255)
                                                                                                                 gCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTgg                                                          >  6:84730/1‑111 (MQ=255)
                                                                                                                 gCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTAt                    >  5:121892/1‑149 (MQ=255)
                                                                                                                   ccTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTg                  >  4:305537/1‑149 (MQ=255)
                                                                                                                       tCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGgtgt              <  6:197624/149‑1 (MQ=255)
                                                                                                                       tCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGgtgt              <  3:305537/149‑1 (MQ=255)
                                                                                                                         aTGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGc            >  3:189902/1‑149 (MQ=255)
                                                                                                                         aTGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGc            <  3:18393/149‑1 (MQ=255)
                                                                                                                          tGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTg                                                   >  1:288195/1‑109 (MQ=255)
                                                                                                                          tGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTg                                                   <  2:288195/109‑1 (MQ=255)
                                                                                                                            gCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTc         <  1:229068/149‑1 (MQ=255)
                                                                                                                            gCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTc         >  7:317330/1‑149 (MQ=255)
                                                                                                                            gCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTc         >  3:72261/1‑149 (MQ=255)
                                                                                                                            gCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTc         >  6:13222/1‑149 (MQ=255)
                                                                                                                                ccccAAAATGCTGCGGCATTGCCGGAGCATCGTGGGGTTGCCCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTc         >  3:73568/1‑145 (MQ=38)
                                                                                                                                ccccAAAATGCTGCGGCATTGCCGGAGCATCGTGGGGTTGCCCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTc         <  4:73568/145‑1 (MQ=38)
                                                                                                                                 cccACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGtt    >  6:31695/1‑149 (MQ=255)
                                                                                                                                 cccACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGATTTCCAGGTCTTCAACCTCACCGCGGTATTGGTGTGCTTCgtgt     >  6:37275/1‑148 (MQ=255)
                                                                                                                                   caaaaTGCTGCGGCAATGCCGGAGCATCGTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGATGTCCAGGTTTTCAAGCTCAACGCGGTATTGGTGTGCTTCCTGTACg  >  8:174878/5‑149 (MQ=38)
                                                                                                                                   cACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCgt       <  6:270189/144‑1 (MQ=255)
                                                                                                                                   cACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCgt       >  5:270189/1‑144 (MQ=255)
                                                                                                                                   cACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTCg  >  3:41517/1‑149 (MQ=255)
                                                                                                                                   cACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTCg  <  4:189902/149‑1 (MQ=255)
                                                                                                                                   cACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTCg  >  8:224043/1‑149 (MQ=255)
                                                                                                                                   cACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTCg  >  6:129620/1‑149 (MQ=255)
                                                                                                                                            |                                                                                                                                           
AGCAAGGTGAGTTGCAGCTGTGCATCAATGACGACCTGGACGGGCGTTACGGGGCTGGCCTGAGCGATAACCTGGGCAGCCTGTCGGTAGAGGTGCGTTTCGGGGCGGTGTAGGCCCTGTCATGGCAACCCCACGATGCTCCGGCAATGCCGCAGCATTTTGGGGTTGCTCACTGAGCCCAGGCGAGTCAGGGGTTCGCCGCAATGGGCAGGGTCATTGTGTGGCGCGCTGGTTTTCCAGGTCTTCAAGCTCAACGCGGTATTGGTGTGCTTCGTGTTCG  >  NC_002947/1858968‑1859247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑