Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,871,088 C→T 8.1% A121V (GCG→GTG)  cobC → adenosylcobinamide‑phosphate synthase subunit beta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,871,0880CT 8.1% 141.0 / 2.7 50A121V (GCG→GTG) cobCadenosylcobinamide‑phosphate synthase subunit beta
Reads supporting (aligned to +/- strand):  ref base C (21/24);  new base T (2/2);  total (24/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

GCTCCCAGGCCGCGATCCAGGCCTTGCCGCTGCTGCGTACAACCGCCCGGGTTGGCGTGCTGACGCCGTGCTATGCCGAACACCCCTATGCCTGGCAACGGGCGGGGCATCAACTGCTGGCGCTGGATGAAGCGCAAGTCGAGGCTGCGCTCGACGGCCTCGATGTGCTGGTGCTGGTGAACCCCAACAACCCGACCGGCCAGCGGGTGTCGCGCGAACGCCTGCTGGCTTGGCATGCTCGCCTGGCTGCCCGCGGTGGCTGGCTGCTGGTCGACGAAGCAT  >  NC_002947/1870941‑1871222
                                                                                                                                                   |                                                                                                                                      
gCTCCCAGGCCGCGATCCAGGCCTTGCCGCTGCTGCGTACAACCGCCCGGGTTGGCGTGCTGACGCCGTGCTATGCCGAACACCCCTATGCCTGGCAACGGGCGGGGCATCAACTGCTGGCGCTGGATGAAGCGCAAGTCGAGGCTgcg                                                                                                                                       >  8:92150/1‑149 (MQ=255)
     cAGGCCGCGATCCAGGCCTTGCCGCTGCTGCGTACAACCGCCCGGGTTGGCGTGCTGACGCCGTGCTATGCCGAACACCCCTATGCCTGGCAACGGGCGGGGCATCAACTGCTGGCGCTGGATGAAGCGCAAGTCGAGGCTGCGCTCGa                                                                                                                                  <  5:80557/149‑1 (MQ=255)
          cgcgATCCAGGCCTTGCCGCTGCTGCGTACAACCGCCCGGGTTGGCGTGCTGACGCCGTGCTATGCCGAACACCCCTATGCCTGGCAACGGGCGGGGCATCAACTGCTGGCGCTGGATGAAGCGCAAGTCGAGGCTGTGCTCGACGGcc                                                                                                                             >  7:349881/1‑149 (MQ=255)
          cgcgATCCAGGCCTTGCCGCTGCTGCGTACAACCGCCCGGGTTGGCGTGCTGACGCCGTGCTATGCCGAACACCCCTATGCCTGGCAACGGGCGGGGCATCAACTGCTGGCGCTGGATGAAGCGCAAGTCGAGGCTGTGCTCGACGGcc                                                                                                                             >  3:156899/1‑149 (MQ=255)
          cgcgATCCAGGCCTTGCCGCTGCTGCGTACAACCGCCCGGGTTGGCGTGCTGACGCCGTGCTATGCCGAACACCCCTATGCCTGGCAACGGGCGGGGCATCAACTGCTGGCGCTGGATGAAGCGCAAGTCGAGGCTGCGCTCGACGGcc                                                                                                                             >  2:11835/1‑149 (MQ=255)
          cgcgATCCAGGCCTTGCCGCTGCTGCGTACAACCGCCCGGGTTGGCGTGCTGACGCCGTGCTATGCCGAACACCCCTATGCCTGGCAACGGGCGGGGCATCAACTGCTGGCGCTGGATGAAGCGCAAGTCGAGGCTGCGCTCGACGGcc                                                                                                                             >  3:302531/1‑149 (MQ=255)
          cgcgATCCAGGCCTTGCCGCTGCTGCGTACAACCGCCCGGGTTGGCGTGCTGACGCCGTGCTATGCCGAACACCCCTATGCCTGGCAACGGACGGGGCATCAACTGCTGGCGCTGGATGAAGCGCAAGTCGAGGCTGCGCTCGACGGcc                                                                                                                             >  1:327126/1‑149 (MQ=255)
               tCCAGGCCTTGCCGCTGCTGCGTACAACCGCCCGGGTTGGCGTGCTGACGCCGTGCTATGCCGAACACCCCTATGCCTGGCAACGGGCGGGGCATCAACTGCTGGCGCTGGATGAAGCGCAAGTCGAGGCTGCGCTCGACGGCCTCGAt                                                                                                                        <  7:92150/149‑1 (MQ=255)
                  aGGCCTTGCCGCTGCTGCGTACAACCGCCCGGGTTGGCGTGCTGACGCCGTGCTATGCCGAACACCCCTATGCCTGGCAACGGGCGGGGCATCAACTGCTGGCGCTGGATGAAGCGCAAGTCGAGGCTGCGCTCGACGGCCTCGAtgtg                                                                                                                     <  4:192543/149‑1 (MQ=255)
                     ccTTGCCGCTGCTGCGTACAACCGCCCGGGTTGGCGTGCTGACGCCGTGCTATGCCGAACACCCCTATGCCTGGCAACGGGCGGGGCATCAACTGCTGGCGCTGGATGAAGCGCAAGTCGAGGCTGCGCTCGACGGCCTCGATgtgctg                                                                                                                  <  7:15409/149‑1 (MQ=255)
                     ccTTGCCGCTGCTGCGTACAACCGCCCGGGTTGGCGTGCTGACGCCGTGCTATGCCGAACACCCCTATGCCTGGCAACGGGCGGGGCATCAACTGCTGGCGCTGGATGAAGCGCAAGTCGAGGCTGCGCTCGACGGCCTCGATgtgctg                                                                                                                  <  4:19405/149‑1 (MQ=255)
                         gCCGCTGCTGCGTACAACCGCCCGGGTTGGCGTGCTGACGCCGTGCTATGCCGAACACCCCTATGCCTGGCAACGGGCGGGGCATCAACTGCTGGCGCTGGATGAAGCGCAAGTCGAGGCTGCGCTCGACGGCCTCGATgtgctggtgc                                                                                                              >  3:40740/1‑149 (MQ=255)
                             ctgctgCGTACAACCGCCCGGGTTGGCGTGCTGACGCCGTGCTATGCCGAACACCCCTATGCCTGGCAACGGGCGGGGCATCAACTGCTGGCGCTGGATGAAGCGCAAGTCGAGGCTGCGCTCGACGGCCTCGATGTgctggtgctggt                                                                                                          <  6:370671/149‑1 (MQ=255)
                                 ggcGTACAACCGCCCGGGTTGGCGTGCTGACGCCGTGCTATGCCGAACACCCCTATGCCTGGCAACGGGCGGGGCATCAACTGCTGGCGCTGGATGAAGCGCAAGTCGAGGCTGCGCTCGACGGCCTCGATGTGCTGGGGCTGGTGAAc                                                                                                      <  2:231827/148‑1 (MQ=255)
                                         aCCGCCCGGGTTGGCGTGCTGACGCCGTGCTATGCCGAACACCCCTATGCCTGGCAACGGGCGGGGCATCAACTGCTGGCGCTGGATGAAGCGCAAGTCGAGGCTGCGCTCGACGGCCTCGATGTGCTGGTGCTGGTGAACCCcaacaa                                                                                              >  7:196296/1‑149 (MQ=255)
                                            gCCCGGGTTGGCGTGCTGACGCCGTGCTATGCCGAACACCCCTATGCCTGGCAACGGGCGGGGCATCAACTGCTGGCGCTGGATGAAGCGCAAGTCGAGGCTGCGCTCGACGGCCTCGATGTGCTGGTGCTGGTGAACCCCAACAAccc                                                                                           >  1:196862/1‑149 (MQ=255)
                                            gCCCGGGTTGGCGTGCTGACGCCGTGCTATGCCGAACACCCCTATGCCTGGCAACGGGCGGGGCATCAACTGCTGGCGCTGGATGAAGCGCAAGTCGAGGCTGCGCTCGACGGCCTCGATGTGCTGGTGCTGGTGAACCCCAACAAccc                                                                                           >  1:214952/1‑149 (MQ=255)
                                              ccGGGTTGGCGTGCTGACGCCGTGCTATGCCGAACACCCCTATGCCTGGCAACGGGCGGGGCATCAACTGCTGGCGCTGGATGAAGCGCAAGTCGAGGCTGCGCTCGACGGCCTCGATGTGCTGGTGCTGGTGAACCCCAACAACCCGa                                                                                         >  6:96799/1‑149 (MQ=255)
                                               cGGGTTGGCGTGCTGACGCCGTGCTATGCCGAACACCCCTATGCCTGGCAACGGGCGGGGCATCAACTGCTGGCGCTGGATGAAGCGCAAGTCGAGGCTGCGCTCGACGGCCTCGATGTGCTGGTGCTGGTGAACCCCAACAACCCGAc                                                                                        <  8:33148/149‑1 (MQ=255)
                                                gggTTGGCGTGCTGACGCCGTGCTATGCCGAACACCCCTATGCCTGGCAACGGGCGGGGCATCAACTGCTGGCGCTGGATGAAGCGCAAGTCGAGGCTGCGCTCGACGGCCTCGATGTGCTGGTGCTGGTGAACCCCAACAACCCGAcc                                                                                       <  2:196862/149‑1 (MQ=255)
                                                      gCGTGCTGACGCCGTGCTATGCCGAACACCCCTATGCCTGGCAACGGGCGGGGCATCAACTGCTGGCGCTGGATGAAGCGCAAGTCGAGGCTGAGCTCGACGGCCTCGATGTGCTGGTGCTGGTGAACCCCAACAACCCGACCGGCCAg                                                                                 >  5:277512/1‑149 (MQ=255)
                                                        gTGCTGACGCCGTGCTATGCCGAACACCCCTATGCCTGGCAACGGGCGGGGCATCAACTGCTGGCGCTGGATGAAGCGCAAGTCGAGGCTGCGCTCGACGGCCTCGATGTGCGGGTGCTGGTGAACCCCAACAACCCGCCCGGACAGCg                                                                               >  6:184606/1‑149 (MQ=255)
                                                         tGCTGACGCCGTGCTATGCCGAACACCCCTATGCCTGGCAACGGGCGGGGCATCAACTGCTGGCGCTGGATGAAGCGCAAGTCGAGGCTGTGCTCGACGGCCTCGATGTGCTGGTGCTGGTGAACCCCAACAACCCGACCGGCCAGCgg                                                                              <  8:349881/149‑1 (MQ=255)
                                                         tGCTGACGCCGTGCTATGCCGAACACCCCTATGCCTGGCAACGGGCGGGGCATCAACTGCTGGCGCTGGATGAAGCGCAAGTCGAGGCTGTGCTCGACGGCCTCGATGTGCTGGTGCTGGTGAACCCCAACAACCCGACCGGCCAGCgg                                                                              <  4:156899/149‑1 (MQ=255)
                                                              aCGCCGTGCTATGCCGAACACCCCTATGCCTGGCAACGGGCGGGGCATCAACTGCTGGCGCTGGATGAAGCGCAAGTCGAGGCTGCGCTCGACGGCCTCGATGTGCTGGTGCTGGTGAACCCCAACAACCCGACCGGCCAGCGGgtgt                                                                          <  2:214952/148‑1 (MQ=255)
                                                                gCCGTGCTATTCCGAACACCCCTATGCCTGGCAACGTGCTGGGCATCAACTGCTGGCGGTGGATGTAGGGAAAGTCGTGGCTGCGCTCGACGGCCTGGATGTGCTGGTGCTGGTGAACCCGAACAACCCGACCGGCCAGCGTGTGTcgc                                                                       <  6:277512/149‑1 (MQ=255)
                                                                         tGCCGAACACCCCTATGCCTGGCAACGGGCGGGGCATCAACTGCTGGCGCTGGATGAAGCGCAAGTCGAGGCTGCGCTCGACGGCCTCGATGTGCTGGTGCTGGTGAACCCCAACAACCCGACCGGCCAGCGGGTGTCGCGCGAACGc                                                               <  5:96799/148‑1 (MQ=255)
                                                                             gAACACCCCTATGCCTGGCAACGGGCGGGGCATCAACTGCTGGCGCTGGATGAAGCGCAAGTCGAGGCTGCGCTCGACGGCCTCGATGTGCTGGTGCTGGTGAACCCCAACAACCCGACCGGCCAGCggg                                                                             <  3:302158/130‑1 (MQ=255)
                                                                             gAACACCCCTATGCCTGGCAACGGGCGGGGCATCAACTGCTGGCGCTGGATGAAGCGCAAGTCGAGGCTGCGCTCGACGGCCTCGATGTGCTGGTGCTGGTGAACCCCAACAACCCGACCGGCCAGCggg                                                                             >  4:302158/1‑130 (MQ=255)
                                                                                   cccTATGCCTGGCAACGGGCGGGGCATCAACTGCTGGCGCTGGATGACGCGCAAGTCGAGGCTGCGCTCGACGGCCTCGATGTGCTGGTGCTGGTGAACCCCAACAACCCGACCGGCCAGCGGGTGTCGCGCGAACGCCTGCTGGCTTg                                                    <  8:238645/149‑1 (MQ=255)
                                                                                   cccTATGCCTGGCAACGGGCGGGGCATCAACTGCTGGCGCTGGATGAAGCGCAAGTCGAGGCTGCGCTCGACGGCCTCGATGTGCTGGTGCTGGTGAACCCCAACAACCCGACCGGCCAGCGGGTGTcgc                                                                       >  5:148040/1‑130 (MQ=255)
                                                                                   cccTATGCCTGGCAACGGGCGGGGCATCAACTGCTGGCGCTGGATGAAGCGCAAGTCGAGGCTGCGCTCGACGGCCTCGATGTGCTGGTGCTGGTGAACCCCAACAACCCGACCGGCCAGCGGGTGTcgc                                                                       <  6:148040/130‑1 (MQ=255)
                                                                                   cccTATGCCTGGCAACGGACGGGGCATCAACTGCTGGCGCTGGATGAAGCGCAAGTCGAGGCTGCGCTCGACGGCCTCGATGTGCTGGTGCTGGTGAACCCCAACAACCCGACCGGCCAGCGGGTGTCGCGCGAACGCCTGCTGGCTTg                                                    <  2:327126/149‑1 (MQ=255)
                                                                                           cTGGCAACGGGCGGGGCATCAACTGCTGGCGCTGGATGAAGCGCAAGTCGAGGCTGCGCTCGACGGCCTCGATGTGCTGGTGCTGGTGAACCCCAACAACCCGACCGGCCAGCGGGTGTc                                                                         >  5:76470/1‑120 (MQ=255)
                                                                                           cTGGCAACGGGCGGGGCATCAACTGCTGGCGCTGGATGAAGCGCAAGTCGAGGCTGCGCTCGACGGCCTCGATGTGCTGGTGCTGGTGAACCCCAACAACCCGACCGGCCAGCGGGTGTc                                                                         <  6:76470/120‑1 (MQ=255)
                                                                                                aaCGGGCGGGGCATCAACTGCTGGCGCTGGATGAAGCGCAAGTCGAGGCTGCGCTCGACGGCCTCGATGTGCTGGTGCTGGTGAACCCCAACAACGCGACCGGCCAGCGGGTGTCGCGCGAACGCCTGCTGGCTTGGCATGCTCGCCTg                                       >  1:302936/1‑149 (MQ=255)
                                                                                                aaCGGGCGGGGCATCAACTGCTGGCGCTGGATGAAGCGCAAGTCGAGGCTGCGCTCGACGGCCTCGATGTGCTGGTGCTGGTGAACCCCAACAACCCGACCGGCCAGCGGGTGTcgcgc                                                                     <  8:274565/119‑1 (MQ=255)
                                                                                                aaCGGGCGGGGCATCAACTGCTGGCGCTGGATGAAGCGCAAGTCGAGGCTGCGCTCGACGGCCTCGATGTGCTGGTGCTGGTGAACCCCAACAACCCGACCGGCCAGCGGGTGTcgcgc                                                                     >  7:274565/1‑119 (MQ=255)
                                                                                                 aCGGGCGGGGCATCAACTGCTGGCGCTGGATGAAGCGCAAGTCGAGGCTGCGCTCGACGGCCTCGATGTGCTGGTGCTGGTGAACCCCAACAACCCGACCGGCCAGCGGGTGTCGCGCGAACGCCTGCTGGCTTGGCATGCTCGCCTgg                                      <  4:40740/149‑1 (MQ=255)
                                                                                                     gcgggGCATCAACTGCTGGCGCTGGATGAAGCGCAAGTCGAGGCTGCGCTCGACGGCCTCGATGTGCTGGTGCTGGTGAACACCAACAACCCGACCGGCCAGCGGGTGTCGCGCGAACGCCTGCTGGCTTGGCATGCTCGCCTGGCTGc                                  >  8:100464/1‑149 (MQ=255)
                                                                                                            aTCAACTGCTGGCGCTGGATGAAGCGCAAGTCGAGGCTGCGCTCGACGGCCTCGATGTGCTGGTGCTGGTGAACCCCAACAACCCGACCGGCCAGCGGGTGTCGCGCGAACGcc                                                              <  2:354636/114‑1 (MQ=255)
                                                                                                            aTCAACTGCTGGCGCTGGATGAAGCGCAAGTCGAGGCTGCGCTCGACGGCCTCGATGTGCTGGTGCTGGTGAACCCCAACAACCCGACCGGCCAGCGGGTGTCGCGCGAACGcc                                                              >  1:354636/1‑114 (MQ=255)
                                                                                                             tCAACTGCTGGCGCTGGATGAAGCGCAAGTCGAGGCTGCGCTCGACGGCCTCGATGTGCTGGTGCTGGTGAACCCCAACAACCCGACCGGCCAGCGGGTGTCGCGCGAACGCCTGCTGGCTTGGCATGCTCGCCTGGCTGCCCGCGGtg                          >  5:7030/1‑149 (MQ=255)
                                                                                                              cAACTGCTGGCGCTGGATGAAGCGCAAGTCGAGGCTGCGCTCGACGGCCTCGATGTGCTGGTGCTGGTGAACCCCAACAACCCGACCGGCCAGCGGGTGTCGCGCGAACGCCTGCTGGCTTGGCATGc                                              <  2:95404/128‑1 (MQ=255)
                                                                                                              cAACTGCTGGCGCTGGATGAAGCGCAAGTCGAGGCTGCGCTCGACGGCCTCGATGTGCTGGTGCTGGTGAACCCCAACAACCCGACCGGCCAGCGGGTGTCGCGCGAACGCCTGCTGGCTTGGCATGc                                              >  1:95404/1‑128 (MQ=255)
                                                                                                               aaCTGCTGGCGCTGGATGAAGCGCAAGTCGAGGCTGCGCTCGACGGCCTCGATGTGCTGGTGCTGGTGAACCCCAACAACCCGACCGGCCAGCGGGTGTCGCGCGAACGCCTGCTGGCTTGGCATGCTCGCCTGGCTGCCCGCGGtggc                        >  8:251798/1‑149 (MQ=255)
                                                                                                                       gcgcTGGATGAAGCGCAAGTCGAGGCTGCGCTCGACGGCCTCGATGTGCTGGTGCTGGTGAACCCCAACAACCCGACCGGCCAGCGGGTGTCGCGCGAACGCCTGCTGGCTTGGCATGCTCGCCTGGCTGCCCGCGGTGGCTGgctgct                <  8:196296/149‑1 (MQ=255)
                                                                                                                       gcgcTGGATGAAGCGCAAGTCGAGGCTGCGCTCGACGGCCTCGATGTGCTGGTGCTGGTGAACCCCAACAACCCGACCGGCCAGCGGGTGTCGCGCGAACGCCTGCTGGCTTGGCATGCTCGCCTGGCTGCCCGCGGTGGCTGgctgc                 <  1:11835/148‑1 (MQ=255)
                                                                                                                              aTGAAGCGCAAGTCGAGGCTGCGCTCGACGGCCTCGATGTGCTGGTGCTGGTGAACCCCAACAACCCGACCGGCCAGCGGGTGTCGCGCGAACGCCTGCTGGCTTGGCATGCTCGCCTGGCTGCCCGCGGTGGCTGGCTGCTGGTcgac         >  4:151646/1‑149 (MQ=255)
                                                                                                                              aTGAAGCGCAAGGCGAGGCTGCGCTCGACGGCCTCGATGTGCTGCTGCGGGTGAACCCCAACAACCCGACCGGCCAGCGGGTGTCGCGCGAACGCCTGCTGGCTTGGCATGCTCGCCTGGCTGCCCGCGGTGGCTGGCTGCTGGGcgac         <  5:184606/149‑1 (MQ=255)
                                                                                                                                     gcAAGTCGAGGCTGCGCTCGACGGCCTCGATGTGCTGGTGCTGGTGAACCCCAACAACCCGACCGGCCAGCGGGTGTCGCGCGAACGCCTGCTGGCTTGGCATGCTCGCCTGGCTGTCCGCGGTGGCTGGCTGCTGGTCGACGAAGCAt  >  2:190119/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                      
GCTCCCAGGCCGCGATCCAGGCCTTGCCGCTGCTGCGTACAACCGCCCGGGTTGGCGTGCTGACGCCGTGCTATGCCGAACACCCCTATGCCTGGCAACGGGCGGGGCATCAACTGCTGGCGCTGGATGAAGCGCAAGTCGAGGCTGCGCTCGACGGCCTCGATGTGCTGGTGCTGGTGAACCCCAACAACCCGACCGGCCAGCGGGTGTCGCGCGAACGCCTGCTGGCTTGGCATGCTCGCCTGGCTGCCCGCGGTGGCTGGCTGCTGGTCGACGAAGCAT  >  NC_002947/1870941‑1871222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑