Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,967,647	C→G	7.2%	G115R (GGC→CGC) ‡	yciK ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,967,647	0	C	G	7.2%	201.4 / 4.7	66	G115R (GGC→CGC) ‡	yciK	putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (34/27);  new base G (2/3);  total (36/30)
Fisher's exact test for biased strand distribution p-value = 6.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.08e-01

CCAGTAAGCCCGGCCCTTGCGCCCCACGCTGCTGCTGGTGAACACCACCGAAGCGTCTTCCGACAGCTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCTCGTCGTACTGGTGTGGCAGGGCGGTTTCCAGGTTGAACGGGA  >  NC_002947/1967501‑1967787                                                                                                                                                    |                                                                                                                                             ccAGTAAGCCCGGCCCTTGCGCCCCACGCTGCTGCTGGTGAACACCACCGAAGCGTCTTCCGACAGCTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGccc                                                                                                                                             >  6:132374/1‑148 (MQ=255)     tAAGCCCGGCCCTTGCGCCCCACGCTGCTGCTGGTGAACACCACCGAAGCGTCTTCCGACAGCTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACaa                                                                                                                                        >  7:45383/1‑149 (MQ=255)      aaGCCCGGCCCTTGCGCCCCACGCTGCTGCTGGTGAACACCACCGAAGCGTCTTCCGACAGCTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAAc                                                                                                                                       <  2:218085/149‑1 (MQ=255)       aGCCCGGCCCTTGCGCCCCACGCTGCTGCTGGTGAACACCACCGAAGCGTCTTCCGACAGCTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAAc                                                                                                                                       <  8:42377/148‑1 (MQ=255)         cccGGCCCTTGCGCCCCACGCTGCTGCTGGTGAACACCACCGAAGCGTCTTCCGACAGCTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGGGCGACAACttc                                                                                                                                    <  2:169246/149‑3 (MQ=255)               ccTTGCGCCCCACGCTGCTGCTGGTGAACACCACCGAAGCGTCTTCCGACAGCTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAgc                                                                                                                              >  8:50312/1‑149 (MQ=255)                    cgcCCCACGCTGCTGCTGGGGAACACCACCGAAGCGTCTTCCGACAGCTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAgcggc                                                                                                                           <  7:183098/147‑1 (MQ=255)                                ctgctgGTGAACACCACCGAAGCGTCTTCCGACAGCTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCga                                                                                                              <  6:251610/148‑1 (MQ=255)                                 tgctgGTGAACACCACCGAAGCGTCTTCCGACAGCTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCgat                                                                                                             >  1:125364/1‑148 (MQ=255)                                 tgctgGTGAACACCACCGAAGCGTCTTCCGACAGCTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCgat                                                                                                             >  6:333469/1‑148 (MQ=255)                                       tGAACACCACCGAAGCGTCTTCCGACAGCTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGAc                                                                                                      >  6:168150/1‑149 (MQ=255)                                       tGAACACCACCGAAGCGTCTTCCGACAGCTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGAc                                                                                                      <  5:132374/149‑1 (MQ=255)                                        gAACACCACCGAAGCGTCTTCCGACAGCTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACg                                                                                                     >  8:140801/1‑149 (MQ=255)                                        gAACACCACCGAACCGTCTTCCGACAGCTGCAGCAGCGGCAGCAGGGTGGTGGTGAGCATGAAGGTGGCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAAATGCTCCACCGGCTTGCGCGGGCCGATGATCGACg                                                                                                     >  8:276588/1‑149 (MQ=255)                                         aaCACCACCGAAGCGTCTTCCGACAGCTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACg                                                                                                     >  3:293570/1‑148 (MQ=255)                                              caccGTAGCGTCTTCCGACAGCTTCAGCAGCGGCAGCAGGGTGCTGGAGAGCATGAAGGTGGCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCAttgt                                                                                               >  5:121808/1‑149 (MQ=255)                                              caccGAAGCGTCTTCCGACAGCTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCgatgat                                                                                                          <  6:240862/138‑1 (MQ=255)                                              caccGAAGCGTCTTCCGACAGCTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCAttgt                                                                                               >  2:2680/1‑149 (MQ=255)                                               accGAAGCGTCTTCCGACAGCTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCAttgtt                                                                                              <  5:333469/149‑1 (MQ=255)                                                      cGTCTTCCGACAGCTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAgg                                                                                       >  2:287106/1‑149 (MQ=255)                                                       gtggtCCGACAGGTTCTGCAGGGGCAGCAGTGTTCTGGGTAGCATGAAGG‑GTCATTTTAGGCTGATGGGCATCACGCGCATGAAGTTTTCGAGCGAGAAATTCTCGTGGGGATTGCGTGTGAGTATGATCGACGCATTGTTGAGCAGGc                                                                                      <  4:160048/145‑1 (MQ=255)                                                        tCTTCCGACAGCTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGGCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGcc                                                                                     <  3:91884/149‑1 (MQ=255)                                                          ttCCGACAGCTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGGGCGACAACttcatg                                                                                                                                 >  3:75058/1‑98 (MQ=255)                                                          ttCCGACAGCTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGGGCGACAACttcatg                                                                                                                                 <  4:75058/103‑6 (MQ=255)                                                            ccGACAGCTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATc                                                                                  <  7:276588/148‑1 (MQ=255)                                                                 aGCTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAggag                                                                            >  1:191382/1‑147 (MQ=255)                                                                   cTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCgatga                                                                                                           <  2:274074/116‑1 (MQ=255)                                                                   cTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCgatga                                                                                                           >  1:274074/1‑116 (MQ=255)                                                                   cTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAggaggc                                                                          >  6:188025/1‑149 (MQ=255)                                                                       agcagcGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGaa                                                                      >  8:127542/1‑149 (MQ=255)                                                                        gcagcGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAAc                                                                     >  7:138669/1‑149 (MQ=255)                                                                            cGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGc                                                                 <  7:140801/149‑1 (MQ=255)                                                                                agcagGGTGCTGGTGAGCATGAAGGTGGCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCAttgtt                                                                                              <  8:36133/116‑1 (MQ=255)                                                                                agcagGGTGCTGGTGAGCATGAAGGTGGCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCAttgtt                                                                                              >  7:36133/1‑116 (MQ=255)                                                                                             tGAGCATGAAGGTGGCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCAttgtt                                                                                              >  1:293062/1‑103 (MQ=255)                                                                                             tGAGCATGAAGGTGGCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCAttgtt                                                                                              <  2:293062/103‑1 (MQ=255)                                                                                                gCATGAAGGTGGCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGc                                             >  1:351635/1‑149 (MQ=255)                                                                                                gCATGAAGGTGGCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGc                                             <  1:287106/149‑1 (MQ=255)                                                                                                  aTGAAGGTGGCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCtc                                           >  6:322104/1‑149 (MQ=255)                                                                                                     aaGGTGGCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCtcgtc                                        >  1:108362/1‑149 (MQ=255)                                                                                                       ggTGGCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCtcgtcg                                       >  1:360742/1‑148 (MQ=255)                                                                                                        gTGGCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCTCGTCGTa                                     >  7:177991/1‑149 (MQ=255)                                                                                                         tGGCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACt                                                                    <  8:114639/117‑1 (MQ=255)                                                                                                         tGGCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACt                                                                    >  7:114639/1‑117 (MQ=255)                                                                                                         tGGCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCTCGTCGTAc                                    <  2:351635/149‑1 (MQ=255)                                                                                                           gCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCTCGTCGTACTg                                  <  1:2680/149‑1 (MQ=255)                                                                                                             a‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCTCGTCGTACTGgt                                >  5:75714/1‑149 (MQ=255)                                                                                                                tGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCTCGTCGTACTGgtgt                              >  5:46926/1‑149 (MQ=255)                                                                                                                 gACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCTCGTCGTACTGgtgt                              <  7:127542/148‑1 (MQ=255)                                                                                                                  aCGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCTAACTGGCCTTCGATCa                                                        <  6:228405/121‑1 (MQ=255)                                                                                                                  aCGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCa                                                        >  5:228405/1‑121 (MQ=255)                                                                                                                  aCGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCTCGTCGTACTGGTGTgg                            >  8:62237/1‑149 (MQ=255)                                                                                                                   cGTTGATCTGCATCACGCTCCTGAAGTTGTCTCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGTCCGAACTGGCCTTCGATCATGGCCGCCAGCTCGTCGTACTGGTGTGGc                           <  8:156204/149‑1 (MQ=255)                                                                                                                    gTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCTCGTCGTACTGGTGTgg                            >  5:267745/1‑147 (MQ=255)                                                                                                                           tgCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCTCGTCGTACTGGTGTGGCAGGGCGGt                   <  2:125364/149‑1 (MQ=255)                                                                                                                             cATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCTCGTCGTACTGGTGTGGCAGGGCGGtt                  <  7:50312/148‑1 (MQ=255)                                                                                                                              aTCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCTCGTCGTACTGGTGTGGCAGGGCGGTTTc                >  7:183025/1‑149 (MQ=255)                                                                                                                              aTCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCTCGTCGTACTGGTGTGGCAGGGCGGTTTc                <  6:75714/149‑1 (MQ=255)                                                                                                                              aTCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCTCGTCGTACTGGTGTGGCAGGGCGGTTTc                >  2:302183/1‑149 (MQ=255)                                                                                                                                 aCGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCTCGTCGTACTTGTGTGGCAGGGCGGTTTCCAg             <  8:177991/149‑1 (MQ=255)                                                                                                                                 aCGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCTCGTCGTACTGGTGTGGCAGGGCGGTTTCCAg             <  8:138669/149‑1 (MQ=255)                                                                                                                                     gcATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCTCGTCGTACTGGTGTGGCAGGGCGGTTTCCAGGtt          >  6:22361/1‑148 (MQ=255)                                                                                                                                     gcATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCCATGATCGAAGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTAGATCATGGCCGCAAGCTCGTCGTACGGGTGTGCCAGGGCGGTATCCAGGtt          >  4:131/1‑148 (MQ=255)                                                                                                                                       aTGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCTCGTCGTACTGGTGTGGCAGGGCGGTTTCCAGGTTGaa       >  4:234583/1‑149 (MQ=255)                                                                                                                                         gcaGTTGTCGGGCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCTCGTCGTACTGGTGTGGCAGGGCGGTTTCCAGGTTGAACg     >  4:176898/3‑149 (MQ=255)                                                                                                                                           aGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCTCGTCGTACTGGTGTGGCAGGGCGGTTTCCAGGTTGAACggg   <  8:45383/149‑1 (MQ=255)                                                                                                                                            gTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATAGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCTCGTCGTACTGGTGTGGCAGGGCGGTTTCCAGGTTGAACGGGa  <  1:302183/149‑1 (MQ=255)                                                                                                                                                    ccGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCtcgtcg                                       >  5:93061/1‑104 (MQ=255)                                                                                                                                                    ccGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCtcgtcg                                       <  6:93061/104‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                             CCAGTAAGCCCGGCCCTTGCGCCCCACGCTGCTGCTGGTGAACACCACCGAAGCGTCTTCCGACAGCTTCAGCAGCGGCAGCAGGGTGCTGGTGAGCATGAAGGTGGCA‑TTGACGTTGATGTGCATCACGCGCATGAAGTTGTCGCCCGACAACTGCTCCAGCGGCGTGCGCGGGCCGATGATCGACGCATTGTTGAGCAGGCCATCTAGGCGGCCGAACTGGCCTTCGATCATGGCCGCCAGCTCGTCGTACTGGTGTGGCAGGGCGGTTTCCAGGTTGAACGGGA  >  NC_002947/1967501‑1967787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑