Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,998,668 A→T 6.7% M271K (ATG→AAG)  rffG ← dTDP‑glucose 4,6‑dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,998,6680AT 6.7% 171.4 / 3.2 60M271K (ATG→AAG) rffGdTDP‑glucose 4,6‑dehydratase
Reads supporting (aligned to +/- strand):  ref base A (30/26);  new base T (2/2);  total (32/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.27e-01

GCCGAGCTCCCGCTCGATCTTGCGTGCATCGATGGCATAACGGCGGTCATGGCCTGGGCGGTCGGTTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGACGGATGCCCGAGCAGTGATCTTCGACATACAGCCAGTCGCG  >  NC_002947/1998520‑1998816
                                                                                                                                                    |                                                                                                                                                    
gCCGAGCTCCCGCTCGATCTTGCGTGCATCGATGGCATAACGGCGGTCATGGCCTGGGCGGTCGGTTACGTAGGCTATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCa                                                                                                                                                      >  4:293258/1‑149 (MQ=255)
         ccGCTCGATCTTGCGTGCATCGATGGCATAACGGCGGTCATGGCCTGGGCGGTCGGTTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCtcgtc                                                                                                                                               <  3:192814/147‑1 (MQ=255)
         ccGCTCGATCTTGCGTGCATCGATGGCATAACGGCGGTCATGGCCTGGGCGGTCGGTTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCtcgtc                                                                                                                                               <  1:265236/147‑1 (MQ=255)
         ccGCTCGATCTTGCGTGCATCGATGGCATAACGGCGGTCATGGCCTGGGCGGTCGGTTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCACAGGTGCCATCtcgtc                                                                                                                                               >  4:321632/1‑147 (MQ=255)
         ccGCTCGATCTTGCGTGCATCGATGGCATAACGGCGGTCATGGCCTGGGCGGTCGGTTACGAAGGCGATCACTTCTGCATAATGTTCGACAGGATCGCCGGTAAACTCATTGATTACCTCGCGCGATCCCGCAGGTGCCATCtcgtcg                                                                                                                                              >  2:292469/1‑148 (MQ=255)
            cTCGATCTTGCGTGCATCGATGGCATAACGGCGGTCATGGCCTGGGCGGTCGGTTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCgag                                                                                                                                            >  1:226594/1‑147 (MQ=255)
                     gCGTGCATCGATGGCATAACGGCGGTCATGGCCTGGGCGGTCGGTTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCTTCTCGACGAGATGGcac                                                                                                                                     <  7:12429/145‑3 (MQ=255)
                     gCGTGCATCGATGGCATAACGGCGGTCATGGCCTGGGCGGTCGGTTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGc                                                                                                                                       <  4:21725/143‑1 (MQ=255)
                           aTCGATGGCATAACGGCGGTCATGGCCTGGGCGGTCGGTTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCTTCTCGACGAGATGGca                                                                                                                                      >  4:345178/1‑137 (MQ=255)
                           aTCGATGGCATAACGGCGGTCATGGCCTGGGCGGTCGGTTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCTTCTCGACGAGATGGca                                                                                                                                      <  3:345178/138‑2 (MQ=255)
                                            ggTCATGGCCTGGGCGGTCGGTTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTg                                                                                                          <  8:108155/149‑1 (MQ=255)
                                                 tGGCCTGGGCGGTCGGTTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCttt                                                                                                     <  6:4590/149‑1 (MQ=255)
                                                     cTGGGCGGTCGGTTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCa                                                                                                 <  3:293258/149‑1 (MQ=255)
                                                        ggCGGTCGGTTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCAtt                                                                                               <  1:59046/148‑1 (MQ=255)
                                                             tcggtTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAgcc                                                                                         <  8:355356/149‑1 (MQ=255)
                                                                    cGTAGGGGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAata                                                                                  <  1:292469/149‑1 (MQ=255)
                                                                     gTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCAGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTAAGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAatat                                                                                 >  3:305928/1‑149 (MQ=255)
                                                                      tAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGCCAATGTTGGCTTTTTCATTCCAGCCGCCAATAtt                                                                                <  2:226594/149‑1 (MQ=255)
                                                                       aGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTg                                                                               >  4:2818/1‑149 (MQ=255)
                                                                         gCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTa                                                                             <  3:2818/149‑1 (MQ=255)
                                                                         gCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCACAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTa                                                                             <  3:321632/149‑1 (MQ=255)
                                                                          cGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTAc                                                                            >  8:61494/1‑149 (MQ=255)
                                                                           gATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGTAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACg                                                                           >  3:334407/1‑149 (MQ=255)
                                                                           gATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACg                                                                           >  7:257137/1‑149 (MQ=255)
                                                                                 ttCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGc                                                                     >  6:31273/1‑149 (MQ=255)
                                                                                 ttCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGAGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTCTACGTCTCGc                                                                     >  1:91029/1‑149 (MQ=255)
                                                                                      cATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTa                                                                             <  3:73010/136‑1 (MQ=255)
                                                                                      cATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTa                                                                             >  4:73010/1‑136 (MQ=255)
                                                                                      cATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAAc                                                                >  2:47088/1‑149 (MQ=255)
                                                                                           tGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCAcc                                                           >  2:230410/1‑149 (MQ=255)
                                                                                           tGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCAcc                                                           >  2:357588/1‑149 (MQ=255)
                                                                                           tGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCAcc                                                           >  3:1446/1‑149 (MQ=255)
                                                                                               cGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCt                                                       >  6:19676/1‑149 (MQ=255)
                                                                                                gtcAGGCTCGCTGGACTTGTGATTGATTACCTGGCGCGATGCCGCAGGTGCGATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAAc                                                                <  3:207320/137‑1 (MQ=255)
                                                                                                gccAGGCTCGCGGGTCTTCTGATTGATTACCTCGCGCGATGCCGCAGCTCCAATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAAACTCAATCTTGGCTAATTCATTGCAGCCTCAAATATTGTACGTCTCCCCTaaa                                                                >  4:207320/3‑138 (MQ=255)
                                                                                                     gCTCGCCGGTCTTCTGATTGATTACCTGGCGGGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAg                                                  >  1:38228/1‑148 (MQ=255)
                                                                                                     gCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCTGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAAGATTGTTCGTCTCGCCGCACGCACCGGCGTCCAg                                                  >  4:235006/1‑148 (MQ=255)
                                                                                                       tCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTAc                                               >  3:274804/1‑149 (MQ=255)
                                                                                                       gagCCTGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTAc                                               >  7:193556/3‑149 (MQ=255)
                                                                                                         gCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTAcgc                                             >  7:269943/1‑149 (MQ=255)
                                                                                                         gCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCAATCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTAcgc                                             >  7:81447/1‑149 (MQ=255)
                                                                                                                    gattgattACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGACGGATGccc                                  >  3:117062/1‑149 (MQ=255)
                                                                                                                    gattgattACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGACGGATGccc                                  >  6:283005/1‑149 (MQ=255)
                                                                                                                     attgattACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGACGGATGccc                                  <  5:19676/148‑1 (MQ=255)
                                                                                                                     attgattACCTGGCGAGAGTGCGAAGGTGCCATCTCGTCGAGAAGGATGTAGAGTGTACGCACAATGGCAATGTTGGATTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGACGGATGc                                    <  4:334407/146‑1 (MQ=255)
                                                                                                                         attACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGACGGATGCCCGAg                               >  5:187900/1‑147 (MQ=255)
                                                                                                                         attACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGACTGCAGAGTGTACGCAAAATGTCAATGTTAGCTTTTTCATTCCAGACACCAATATTGTACGTCTCCACGAACCCAACAGCTTCCACTACCCCACGAATGCCCGacc                              >  2:142370/1‑146 (MQ=255)
                                                                                                                            aCCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGACGGATGCCCGAGCAGt                           <  1:230410/148‑1 (MQ=255)
                                                                                                                             ccTGGCGCGATGCCGGAGGTGCGATCTCGTCGAGTAGGCTGCAGTGTGTACGCACAATGTGAATGTTGGCTTTTTCATTCCAGCCGCCTATATTGTACGTCTCGCCGAACGCAGCGGCTTCCAGTACGCGACGGATGCCCGAGCAGTg                          <  4:305928/148‑1 (MQ=255)
                                                                                                                             ccTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCAATCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGACGGATGCCCGAGCAGTg                          <  8:81447/148‑1 (MQ=255)
                                                                                                                                 gcgcGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGACGGATGCCCGAGCAGTGATCtt                     <  5:77581/149‑1 (MQ=255)
                                                                                                                                  cgcgATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGACGGATGCCCGAGCAGTGATCTTc                    <  8:269943/149‑1 (MQ=255)
                                                                                                                                   gcgATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGACGGATGCCCGAGCAGTGATCTTCg                   <  2:38228/149‑1 (MQ=255)
                                                                                                                                         ccGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGACGGATGCCCGAGCAg                            <  3:81091/134‑1 (MQ=255)
                                                                                                                                         ccGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGACGGATGCCCGAGCAGTGATCTTCGACATAc             <  1:357588/149‑1 (MQ=255)
                                                                                                                                         ccGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGACAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGACGGATGCCCGAGCAg                            >  4:81091/1‑134 (MQ=255)
                                                                                                                                           gCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTcc                                                    >  6:293801/1‑108 (MQ=255)
                                                                                                                                           gCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTcc                                                    <  5:293801/108‑1 (MQ=255)
                                                                                                                                             tgcagCCTTCTCGACGAGATGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGACGGATGCCCGAGCAGTGATCTTCGACATACAGc          >  7:207594/5‑148 (MQ=255)
                                                                                                                                               gTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGcc                                                                    >  6:147958/1‑88 (MQ=255)
                                                                                                                                               gTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGcc                                                                    <  5:147958/88‑1 (MQ=255)
                                                                                                                                                    aTCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGACGGATGCCCGAGCAGTGATCTTCGACATACAGCCAGTcgcg  <  8:257137/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GCCGAGCTCCCGCTCGATCTTGCGTGCATCGATGGCATAACGGCGGTCATGGCCTGGGCGGTCGGTTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGACGGATGCCCGAGCAGTGATCTTCGACATACAGCCAGTCGCG  >  NC_002947/1998520‑1998816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑