| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,998,674 | T→A | 6.9% | D269V (GAC→GTC) | rffG ← | dTDP‑glucose 4,6‑dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,998,674 | 0 | T | A | 6.9% | 165.5 / 3.3 | 58 | D269V (GAC→GTC) | rffG | dTDP‑glucose 4,6‑dehydratase |
| Reads supporting (aligned to +/- strand): ref base T (29/25); new base A (2/2); total (31/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.37e-01 | |||||||||||
CCGCTCGATCTTGCGTGCATCGATGGCATAACGGCGGTCATGGCCTGGGCGGTCGGTTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGACGGATGCCCGAGCAGTGATCTTCGACATACAGCCAGTCGCGG > NC_002947/1998529‑1998817 | ccGCTCGATCTTGCGTGCATCGATGGCATAACGGCGGTCATGGCCTGGGCGGTCGGTTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCtcgtc < 3:192814/147‑1 (MQ=255)ccGCTCGATCTTGCGTGCATCGATGGCATAACGGCGGTCATGGCCTGGGCGGTCGGTTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCtcgtc < 1:265236/147‑1 (MQ=255)ccGCTCGATCTTGCGTGCATCGATGGCATAACGGCGGTCATGGCCTGGGCGGTCGGTTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCACAGGTGCCATCtcgtc > 4:321632/1‑147 (MQ=255)ccGCTCGATCTTGCGTGCATCGATGGCATAACGGCGGTCATGGCCTGGGCGGTCGGTTACGAAGGCGATCACTTCTGCATAATGTTCGACAGGATCGCCGGTAAACTCATTGATTACCTCGCGCGATCCCGCAGGTGCCATCtcgtcg > 2:292469/1‑148 (MQ=255) cTCGATCTTGCGTGCATCGATGGCATAACGGCGGTCATGGCCTGGGCGGTCGGTTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCgag > 1:226594/1‑147 (MQ=255) gCGTGCATCGATGGCATAACGGCGGTCATGGCCTGGGCGGTCGGTTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCTTCTCGACGAGATGGcac < 7:12429/145‑3 (MQ=255) gCGTGCATCGATGGCATAACGGCGGTCATGGCCTGGGCGGTCGGTTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGc < 4:21725/143‑1 (MQ=255) aTCGATGGCATAACGGCGGTCATGGCCTGGGCGGTCGGTTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCTTCTCGACGAGATGGca > 4:345178/1‑137 (MQ=255) aTCGATGGCATAACGGCGGTCATGGCCTGGGCGGTCGGTTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCTTCTCGACGAGATGGca < 3:345178/138‑2 (MQ=255) ggTCATGGCCTGGGCGGTCGGTTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTg < 8:108155/149‑1 (MQ=255) tGGCCTGGGCGGTCGGTTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCttt < 6:4590/149‑1 (MQ=255) cTGGGCGGTCGGTTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCa < 3:293258/149‑1 (MQ=255) ggCGGTCGGTTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCAtt < 1:59046/148‑1 (MQ=255) tcggtTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAgcc < 8:355356/149‑1 (MQ=255) cGTAGGGGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAata < 1:292469/149‑1 (MQ=255) gTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCAGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTAAGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAatat > 3:305928/1‑149 (MQ=255) tAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGCCAATGTTGGCTTTTTCATTCCAGCCGCCAATAtt < 2:226594/149‑1 (MQ=255) aGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTg > 4:2818/1‑149 (MQ=255) gCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTa < 3:2818/149‑1 (MQ=255) gCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCACAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTa < 3:321632/149‑1 (MQ=255) cGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTAc > 8:61494/1‑149 (MQ=255) gATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGTAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACg > 3:334407/1‑149 (MQ=255) gATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACg > 7:257137/1‑149 (MQ=255) ttCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGc > 6:31273/1‑149 (MQ=255) ttCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGAGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTCTACGTCTCGc > 1:91029/1‑149 (MQ=255) cATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTa < 3:73010/136‑1 (MQ=255) cATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTa > 4:73010/1‑136 (MQ=255) cATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAAc > 2:47088/1‑149 (MQ=255) tGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCAcc > 3:1446/1‑149 (MQ=255) tGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCAcc > 2:357588/1‑149 (MQ=255) tGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCAcc > 2:230410/1‑149 (MQ=255) cGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCt > 6:19676/1‑149 (MQ=255) gtcAGGCTCGCTGGACTTGTGATTGATTACCTGGCGCGATGCCGCAGGTGCGATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAAc < 3:207320/137‑1 (MQ=255) gccAGGCTCGCGGGTCTTCTGATTGATTACCTCGCGCGATGCCGCAGCTCCAATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAAACTCAATCTTGGCTAATTCATTGCAGCCTCAAATATTGTACGTCTCCCCTaaa > 4:207320/3‑138 (MQ=255) gCTCGCCGGTCTTCTGATTGATTACCTGGCGGGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAg > 1:38228/1‑148 (MQ=255) gCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCTGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAAGATTGTTCGTCTCGCCGCACGCACCGGCGTCCAg > 4:235006/1‑148 (MQ=255) tCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTAc > 3:274804/1‑149 (MQ=255) gagCCTGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTAc > 7:193556/3‑149 (MQ=255) gCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTAcgc > 7:269943/1‑149 (MQ=255) gCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCAATCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTAcgc > 7:81447/1‑149 (MQ=255) gattgattACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGACGGATGccc > 3:117062/1‑149 (MQ=255) gattgattACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGACGGATGccc > 6:283005/1‑149 (MQ=255) attgattACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGACGGATGccc < 5:19676/148‑1 (MQ=255) attgattACCTGGCGAGAGTGCGAAGGTGCCATCTCGTCGAGAAGGATGTAGAGTGTACGCACAATGGCAATGTTGGATTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGACGGATGc < 4:334407/146‑1 (MQ=255) attACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGACGGATGCCCGAg > 5:187900/1‑147 (MQ=255) attACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGACTGCAGAGTGTACGCAAAATGTCAATGTTAGCTTTTTCATTCCAGACACCAATATTGTACGTCTCCACGAACCCAACAGCTTCCACTACCCCACGAATGCCCGacc > 2:142370/1‑146 (MQ=255) aCCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGACGGATGCCCGAGCAGt < 1:230410/148‑1 (MQ=255) ccTGGCGCGATGCCGGAGGTGCGATCTCGTCGAGTAGGCTGCAGTGTGTACGCACAATGTGAATGTTGGCTTTTTCATTCCAGCCGCCTATATTGTACGTCTCGCCGAACGCAGCGGCTTCCAGTACGCGACGGATGCCCGAGCAGTg < 4:305928/148‑1 (MQ=255) ccTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCAATCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGACGGATGCCCGAGCAGTg < 8:81447/148‑1 (MQ=255) gcgcGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGACGGATGCCCGAGCAGTGATCtt < 5:77581/149‑1 (MQ=255) cgcgATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGACGGATGCCCGAGCAGTGATCTTc < 8:269943/149‑1 (MQ=255) gcgATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGACGGATGCCCGAGCAGTGATCTTCg < 2:38228/149‑1 (MQ=255) ccGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGACGGATGCCCGAGCAg < 3:81091/134‑1 (MQ=255) ccGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGACGGATGCCCGAGCAGTGATCTTCGACATAc < 1:357588/149‑1 (MQ=255) ccGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGACAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGACGGATGCCCGAGCAg > 4:81091/1‑134 (MQ=255) gCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTcc > 6:293801/1‑108 (MQ=255) gCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTcc < 5:293801/108‑1 (MQ=255) tgcagCCTTCTCGACGAGATGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGACGGATGCCCGAGCAGTGATCTTCGACATACAGc > 7:207594/5‑148 (MQ=255) gTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGcc > 6:147958/1‑88 (MQ=255) gTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGcc < 5:147958/88‑1 (MQ=255) aTCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGACGGATGCCCGAGCAGTGATCTTCGACATACAGCCAGTcgcg < 8:257137/149‑1 (MQ=255) tctcGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAATGCACCGGCTTCCAGTACGCGACGGATGCCCGAGCAGTGATCTTCGACATACAGCCAGTCGCgg > 8:144033/1‑149 (MQ=255) gtcgAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGACGGATGCCCGAGCAGTGATCTTCGACATACAGCCAGTCGCgg < 7:61494/145‑1 (MQ=255) | CCGCTCGATCTTGCGTGCATCGATGGCATAACGGCGGTCATGGCCTGGGCGGTCGGTTACGTAGGCGATGAGTTCTGCATACTGTTCGACAGGCTCGCCGGTCTTCTGATTGATTACCTGGCGCGATGCCGCAGGTGCCATCTCGTCGAGAAGGCTGCAGAGTGTACGCACAATGTCAATGTTGGCTTTTTCATTCCAGCCGCCAATATTGTACGTCTCGCCGAACGCACCGGCTTCCAGTACGCGACGGATGCCCGAGCAGTGATCTTCGACATACAGCCAGTCGCGG > NC_002947/1998529‑1998817 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |