Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,021,771 G→C 9.0% intergenic (+308/‑131) PP_1798 → / → gmd outer membrane efflux protein/GDP‑mannose 4,6‑dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,021,7710GC 9.0% 149.9 / 4.9 53intergenic (+308/‑131)PP_1798/gmdouter membrane efflux protein/GDP‑mannose 4,6‑dehydratase
Reads supporting (aligned to +/- strand):  ref base G (29/17);  new base C (3/2);  total (33/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.72e-01

TGCTATGGGGCTATTTCAGATGCTTCCTACAAGTGCCGTGCTGGAACAGCCCTTCGACGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGTTGGAATTGCCCGCGTCAGGCCAAATAAACACACATGCCCGCACGGCA  >  NC_002947/2021631‑2021915
                                                                                                                                            |                                                                                                                                                
tgctATGGGGCTATTTCAGATGCTTCCTACAAGTGCCGTGCTGGAACAGCCCTTCGACGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGa                                                                                                                                           >  1:217056/1‑148 (MQ=255)
   tATGGGGCTATTTCAGATGCTTCCTACAAGTGCCGTGCTGGAACAGCCCTTCGACGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACtt                                                                                                                                       >  5:112348/1‑149 (MQ=255)
       gggCTATTTCAGATGCTTCCTACAAGTGCCGTGCTGGAACAGCCCTTCGACGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTgcgc                                                                                                                                   >  5:139668/1‑149 (MQ=255)
       gggCTATTTCAGATGCTTCCTACAAGTGCCGTGCTGGAACAGCCCTTCGACGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCACCGGCCGATATTgca                                                                                                                                    >  1:130334/1‑147 (MQ=255)
       gggCTATTTCAGATGCTTCCTACAAGTGCCGTGCTGGAACAGCCCTTCGACGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCACCGGCCGATATTgca                                                                                                                                    <  2:130334/148‑2 (MQ=255)
        ggCTATTTCAGATGCTTCCTACAAGTGCCGTGCTGGAACAGCCCTTCGACGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGcc                                                                                                                                  >  4:337869/1‑149 (MQ=255)
         gCTATTTCAGATGCTTCCTACAAGTGCCGTGCTGGAACAGCCCTTCGACGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCg                                                                                                                                 <  6:139668/149‑1 (MQ=255)
                  gATGCTTCCTACAAGTGCCGTGCTGGAACAGCCCTTCGACGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGcc                                                                                                                         >  4:280317/1‑148 (MQ=255)
                  gATGCTTCCTACAAGTGCCGTGCTGGAACAGCCCTTCGACGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAAGTTCGGCCACCGGCCGATATTgcataac                                                                                                                                >  6:121975/1‑136 (MQ=255)
                  gATGCTTCCTACAAGTGCCGTGCTGGAACAGCCCTTCGACGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAAGTTCGGCCACCGGCCGATATTgcataac                                                                                                                                <  5:121975/141‑6 (MQ=255)
                    tGCTTCCTACAAGTGCCGTGCTTGAACAGCCCTTCGACGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAAc                                                                                                                      <  2:217056/149‑1 (MQ=255)
                      cTTCCTACAAGTGCCGTGCTGGAACAGCCCTTCGACGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACa                                                                                                                     <  5:321203/148‑1 (MQ=255)
                       ttCCTACAAGTGCCGTGCTGGAACAGCCCTTCGACGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATc                                                                                                                   >  3:171736/1‑149 (MQ=255)
                       ttCCTACAAGTGCCGTGCTGGAACAGCCCTTCGACGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATc                                                                                                                   >  3:177447/1‑149 (MQ=255)
                           tACAAGTGCCGTGCTGGAACAGCCCTTCGACGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGtt                                                                                                               >  8:189483/1‑149 (MQ=255)
                                    cGTGCTGGAACAGCCCTTCGACGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGcgagtc                                                                                                       >  5:346710/1‑148 (MQ=255)
                                              cAGCCCTTCGACGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGc                                                                                            <  3:280317/149‑1 (MQ=255)
                                                 cccTTCGACGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGccc                                                                                          >  1:78374/1‑148 (MQ=255)
                                                 cccTTCGACGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCg                                                                                         >  2:186529/1‑149 (MQ=255)
                                                   cTTCGACGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGAc                                                                                       <  4:51305/149‑1 (MQ=255)
                                                    ttCGACGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGTTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGAc                                                                                       >  8:250271/1‑148 (MQ=255)
                                                         cGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGAGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATg                                                                                  >  7:52439/1‑148 (MQ=255)
                                                                    cTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTc                                                                       <  1:186529/148‑1 (MQ=255)
                                                                    cTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCg                                                                      >  4:111160/1‑149 (MQ=255)
                                                                      gATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGc                                                                                                                          <  4:23643/95‑1 (MQ=255)
                                                                      gATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGc                                                                                                                          >  3:23643/1‑95 (MQ=255)
                                                                       aTGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGt                                                                     <  6:112348/147‑1 (MQ=255)
                                                                             cTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGta                                                                                >  1:293161/1‑130 (MQ=255)
                                                                             cTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGta                                                                                <  2:293161/130‑1 (MQ=255)
                                                                              tttCGTGCGCGTGCCCCACTTGCTTTGCGCACGCGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCCCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCtt                                                             >  2:259600/1‑148 (MQ=255)
                                                                                  gTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATAc                                                        <  2:78374/149‑1 (MQ=255)
                                                                                   tGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATAcg                                                       >  4:142525/1‑149 (MQ=255)
                                                                                      gcgTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGt                                                    <  4:171736/149‑1 (MQ=255)
                                                                                      gcgTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGt                                                    <  4:177447/149‑1 (MQ=255)
                                                                                           ccccACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGtt                                                <  6:346710/148‑1 (MQ=255)
                                                                                           ccccACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGTTg                                               >  3:268713/1‑149 (MQ=255)
                                                                                                   gCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGTTGGAATTGcc                                       >  7:23855/1‑149 (MQ=255)
                                                                                                    cTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGTTGGAATTGcc                                       <  3:142525/148‑1 (MQ=255)
                                                                                                          gcACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGTTGGAATTGCCCGCGt                                  >  6:277671/1‑147 (MQ=255)
                                                                                                          gcACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGTTGGAATTGCCCGCGt                                  >  5:121474/1‑147 (MQ=255)
                                                                                                           cACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGTTGGAATTGCCCGCGTCAg                               >  6:8806/1‑149 (MQ=255)
                                                                                                                      cAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGTTGGAATTGCCCGCGTCAGGCCAAATaaa                     >  4:190657/1‑148 (MQ=255)
                                                                                                                      cAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGTTGGAATTGCCCGCGTCAGGCCAAATaaa                     >  5:135055/1‑148 (MQ=255)
                                                                                                                        aGGTTTTGCAATATGGGCCGTTTGCCGAACTTGATCCGTGTTAGCCAACATCTGTTTGAGAGTCGAGTTCTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGTTGGAATTGCCCGCGTCAGGCCAAATAAacac                  <  7:250271/149‑1 (MQ=255)
                                                                                                                        aGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGTTGGAATTGCCCGCGTCAGGCCAAATAAacac                  <  5:8806/149‑1 (MQ=255)
                                                                                                                        aGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGTTGGAATTGCCCGCGTCAGGCCAAATAAaca                   >  1:261760/1‑148 (MQ=255)
                                                                                                                             aTGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGTTGGAATTGCCCGCGTCAGGCCAAATAAACACACATg             >  6:2969/1‑149 (MQ=255)
                                                                                                                              tGCAATATCGGCCGCTGGCCTAACTTGCGCCGGGGTAGCAAACATCTGTTTGCGAGTCGAGTACTCAGCCCGACCAATGTATACTTCCTCGTCTAACCTTCATAAGCGTCGTTGGAATTCCCCGCGACAGGCCAAATAAACACACATga            >  8:144093/1‑148 (MQ=255)
                                                                                                                                cAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGTTGGAATTGCCCGCGTCAGGCCAAATAAACACACATGccc          >  7:15892/1‑149 (MQ=255)
                                                                                                                                 aaTATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCACCATCTGTTTGCGAGTCGAGTACTCCGCCCGCCCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGTTGGAATTGCCCGCCTCAGGCCAACTAAACACACATGCCCg         >  7:234333/1‑149 (MQ=255)
                                                                                                                                 aaTATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGTTGGAATTGCCCGCGTCAgg                              <  1:144579/128‑1 (MQ=255)
                                                                                                                                  atatCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGAGCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGTTGGAATTGCCCGCGTCAGGCCAAATAAACACACATGCCCg         >  1:224129/1‑148 (MQ=255)
                                                                                                                                        gCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGTTGGAATTGCCCGCGTCAGGCCAAATAAACACACATGCCCGCACGGCa  <  8:52439/149‑1 (MQ=255)
                                                                                                                                            gTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGTTGGAATTGCCCGCGTCAGGCCAAATAAacacac                >  2:131585/1‑131 (MQ=255)
                                                                                                                                            |                                                                                                                                                
TGCTATGGGGCTATTTCAGATGCTTCCTACAAGTGCCGTGCTGGAACAGCCCTTCGACGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGTTGGAATTGCCCGCGTCAGGCCAAATAAACACACATGCCCGCACGGCA  >  NC_002947/2021631‑2021915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑