Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,021,780 C→A 6.8% intergenic (+317/‑122) PP_1798 → / → gmd outer membrane efflux protein/GDP‑mannose 4,6‑dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,021,7800CA 6.8% 187.3 / 2.4 58intergenic (+317/‑122)PP_1798/gmdouter membrane efflux protein/GDP‑mannose 4,6‑dehydratase
Reads supporting (aligned to +/- strand):  ref base C (34/20);  new base A (2/2);  total (36/22)
Fisher's exact test for biased strand distribution p-value = 6.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01

TATGGGGCTATTTCAGATGCTTCCTACAAGTGCCGTGCTGGAACAGCCCTTCGACGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGTTGGAATTGCCCGCGTCAGGCCAAATAAACACACATGCCCGCACGGCACTATCGGA  >  NC_002947/2021634‑2021923
                                                                                                                                                  |                                                                                                                                               
tATGGGGCTATTTCAGATGCTTCCTACAAGTGCCGTGCTGGAACAGCCCTTCGACGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACtt                                                                                                                                               >  5:112348/1‑149 (MQ=255)
    gggCTATTTCAGATGCTTCCTACAAGTGCCGTGCTGGAACAGCCCTTCGACGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTgcgc                                                                                                                                           >  5:139668/1‑149 (MQ=255)
    gggCTATTTCAGATGCTTCCTACAAGTGCCGTGCTGGAACAGCCCTTCGACGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCACCGGCCGATATTgca                                                                                                                                            >  1:130334/1‑147 (MQ=255)
    gggCTATTTCAGATGCTTCCTACAAGTGCCGTGCTGGAACAGCCCTTCGACGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCACCGGCCGATATTgca                                                                                                                                            <  2:130334/148‑2 (MQ=255)
     ggCTATTTCAGATGCTTCCTACAAGTGCCGTGCTGGAACAGCCCTTCGACGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGcc                                                                                                                                          >  4:337869/1‑149 (MQ=255)
      gCTATTTCAGATGCTTCCTACAAGTGCCGTGCTGGAACAGCCCTTCGACGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCg                                                                                                                                         <  6:139668/149‑1 (MQ=255)
               gATGCTTCCTACAAGTGCCGTGCTGGAACAGCCCTTCGACGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGcc                                                                                                                                 >  4:280317/1‑148 (MQ=255)
               gATGCTTCCTACAAGTGCCGTGCTGGAACAGCCCTTCGACGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAAGTTCGGCCACCGGCCGATATTgcataac                                                                                                                                        <  5:121975/141‑6 (MQ=255)
               gATGCTTCCTACAAGTGCCGTGCTGGAACAGCCCTTCGACGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAAGTTCGGCCACCGGCCGATATTgcataac                                                                                                                                        >  6:121975/1‑136 (MQ=255)
                 tGCTTCCTACAAGTGCCGTGCTTGAACAGCCCTTCGACGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAAc                                                                                                                              <  2:217056/149‑1 (MQ=255)
                   cTTCCTACAAGTGCCGTGCTGGAACAGCCCTTCGACGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACa                                                                                                                             <  5:321203/148‑1 (MQ=255)
                    ttCCTACAAGTGCCGTGCTGGAACAGCCCTTCGACGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATc                                                                                                                           >  3:171736/1‑149 (MQ=255)
                    ttCCTACAAGTGCCGTGCTGGAACAGCCCTTCGACGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATc                                                                                                                           >  3:177447/1‑149 (MQ=255)
                        tACAAGTGCCGTGCTGGAACAGCCCTTCGACGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGtt                                                                                                                       >  8:189483/1‑149 (MQ=255)
                                 cGTGCTGGAACAGCCCTTCGACGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGcgagtc                                                                                                               >  5:346710/1‑148 (MQ=255)
                                           cAGCCCTTCGACGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGc                                                                                                    <  3:280317/149‑1 (MQ=255)
                                              cccTTCGACGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGccc                                                                                                  >  1:78374/1‑148 (MQ=255)
                                              cccTTCGACGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCg                                                                                                 >  2:186529/1‑149 (MQ=255)
                                                cTTCGACGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGAc                                                                                               <  4:51305/149‑1 (MQ=255)
                                                 ttCGACGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGTTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGAc                                                                                               >  8:250271/1‑148 (MQ=255)
                                                      cGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGAGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATg                                                                                          >  7:52439/1‑148 (MQ=255)
                                                                 cTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTc                                                                               <  1:186529/148‑1 (MQ=255)
                                                                 cTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCg                                                                              >  4:111160/1‑149 (MQ=255)
                                                                   gATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGc                                                                                                                                  >  3:23643/1‑95 (MQ=255)
                                                                   gATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGc                                                                                                                                  <  4:23643/95‑1 (MQ=255)
                                                                    aTGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGt                                                                             <  6:112348/147‑1 (MQ=255)
                                                                          cTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGta                                                                                        <  2:293161/130‑1 (MQ=255)
                                                                          cTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGta                                                                                        >  1:293161/1‑130 (MQ=255)
                                                                           tttCGTGCGCGTGCCCCACTTGCTTTGCGCACGCGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCCCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCtt                                                                     >  2:259600/1‑148 (MQ=255)
                                                                               gTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATAc                                                                <  2:78374/149‑1 (MQ=255)
                                                                                tGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATAcg                                                               >  4:142525/1‑149 (MQ=255)
                                                                                   gcgTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGt                                                            <  4:171736/149‑1 (MQ=255)
                                                                                   gcgTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGt                                                            <  4:177447/149‑1 (MQ=255)
                                                                                        ccccACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGtt                                                        <  6:346710/148‑1 (MQ=255)
                                                                                        ccccACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGTTg                                                       >  3:268713/1‑149 (MQ=255)
                                                                                                gCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGTTGGAATTGcc                                               >  7:23855/1‑149 (MQ=255)
                                                                                                 cTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGTTGGAATTGcc                                               <  3:142525/148‑1 (MQ=255)
                                                                                                       gcACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGTTGGAATTGCCCGCGt                                          >  5:121474/1‑147 (MQ=255)
                                                                                                       gcACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGTTGGAATTGCCCGCGt                                          >  6:277671/1‑147 (MQ=255)
                                                                                                        cACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGTTGGAATTGCCCGCGTCAg                                       >  6:8806/1‑149 (MQ=255)
                                                                                                                   cAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGTTGGAATTGCCCGCGTCAGGCCAAATaaa                             >  5:135055/1‑148 (MQ=255)
                                                                                                                   cAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGTTGGAATTGCCCGCGTCAGGCCAAATaaa                             >  4:190657/1‑148 (MQ=255)
                                                                                                                     aGGTTTTGCAATATGGGCCGTTTGCCGAACTTGATCCGTGTTAGCCAACATCTGTTTGAGAGTCGAGTTCTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGTTGGAATTGCCCGCGTCAGGCCAAATAAacac                          <  7:250271/149‑1 (MQ=255)
                                                                                                                     aGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGTTGGAATTGCCCGCGTCAGGCCAAATAAacac                          <  5:8806/149‑1 (MQ=255)
                                                                                                                     aGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGTTGGAATTGCCCGCGTCAGGCCAAATAAaca                           >  1:261760/1‑148 (MQ=255)
                                                                                                                          aTGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGTTGGAATTGCCCGCGTCAGGCCAAATAAACACACATg                     >  6:2969/1‑149 (MQ=255)
                                                                                                                           tGCAATATCGGCCGCTGGCCTAACTTGCGCCGGGGTAGCAAACATCTGTTTGCGAGTCGAGTACTCAGCCCGACCAATGTATACTTCCTCGTCTAACCTTCATAAGCGTCGTTGGAATTCCCCGCGACAGGCCAAATAAACACACATga                    >  8:144093/1‑148 (MQ=255)
                                                                                                                             cAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGTTGGAATTGCCCGCGTCAGGCCAAATAAACACACATGccc                  >  7:15892/1‑149 (MQ=255)
                                                                                                                              aaTATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCACCATCTGTTTGCGAGTCGAGTACTCCGCCCGCCCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGTTGGAATTGCCCGCCTCAGGCCAACTAAACACACATGCCCg                 >  7:234333/1‑149 (MQ=255)
                                                                                                                              aaTATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGTTGGAATTGCCCGCGTCAgg                                      <  1:144579/128‑1 (MQ=255)
                                                                                                                               atatCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGAGCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGTTGGAATTGCCCGCGTCAGGCCAAATAAACACACATGCCCg                 >  1:224129/1‑148 (MQ=255)
                                                                                                                                     gCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGTTGGAATTGCCCGCGTCAGGCCAAATAAACACACATGCCCGCACGGCa          <  8:52439/149‑1 (MQ=255)
                                                                                                                                         gTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGTTGGAATTGCCCGCGTCAGGCCAAATAAacacac                        >  2:131585/1‑131 (MQ=255)
                                                                                                                                             ccGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGTTGGAATTGCCCGCGTCAGGCCAAATAAACACGCATGCCCGCACGGCACTATCg    >  2:192949/1‑147 (MQ=255)
                                                                                                                                             ccGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGTTGGAATTGCCCGCGTCAGGCCAAATAAACACACATGCCCGCACGGCACTATCGGa  >  6:96510/1‑149 (MQ=255)
                                                                                                                                             ccGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGTTGGAATTGCCCGCGTCAGGCCAAATAAACACACATGCCCGCACGGCACTATCGGa  <  3:337869/149‑1 (MQ=255)
                                                                                                                                                aaCTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGTTGGAAtt                                                  <  4:279559/98‑1 (MQ=255)
                                                                                                                                                aaCTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGTTGGAAtt                                                  >  3:279559/1‑98 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
TATGGGGCTATTTCAGATGCTTCCTACAAGTGCCGTGCTGGAACAGCCCTTCGACGGCACGTATCCTGATGCGCCTTTCGTGCGCGTGCCCCACTTGCTTTGCGCACGGGGGTGCCAAGGTTATGCAATATCGGCCGGTGGCCGAACTTGCGCCGGGGTAGCCAACATCTGTTTGCGAGTCGAGTACTCCGCCCGACCTATGTATACTTCCTCGTCTAACCTTCATACGCGTGGTTGGAATTGCCCGCGTCAGGCCAAATAAACACACATGCCCGCACGGCACTATCGGA  >  NC_002947/2021634‑2021923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑