| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,033,379 | T→G | 12.5% | L287R (CTG→CGG) | pgi‑I → | glucose 6‑phosphate isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,033,379 | 0 | T | G | 12.5% | 97.6 / 7.4 | 40 | L287R (CTG→CGG) | pgi‑I | glucose 6‑phosphate isomerase |
| Reads supporting (aligned to +/- strand): ref base T (21/14); new base G (3/2); total (24/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01 | |||||||||||
TACCGCCACTTCATTGCCGTATCCAGCAACAAGGCCGCTGCTGTAGCGTTCGGTATCCGCGAAGAAAACATCTTCCCGATGTGGGACTGGGTGGGTGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGTGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCGTGTGGTATGGCAACTTCTGGGAC > NC_002947/2033240‑2033518 | tACCGCCACTTCATTGCCGTATCCAGCAACAAGGCCGCTGCTGTAGCGTTCGGTATCCGCGAAGAAAACATCTTCCCGATGTCGGACTGGGTGGGTGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCgg > 1:186923/1‑149 (MQ=21) ccGTATCCAGCAACAAGGCCGCTGCTGTAGCGTTCGGTATCCGCGAAGAAAACATCTTCCCGATGTGGGACTGGGTGGGTGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAg > 2:308364/1‑149 (MQ=34) tCCAGCAACAAGGCCGCTGCTGTAGCGTTCGGTATCCGCGAAGAAAACATCTTCCCGATGTGGGACTGGGTGGGTGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAAct > 3:42493/1‑149 (MQ=34) cGCTGCTGTAGCGTTCGGTATCCGCGAAGAAAACATCTTCCCGATGTGGGACTGGGTGGGTGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGTGcc > 1:194045/1‑148 (MQ=37) ctgTAGCGTTCGGTATCCGCGAAGAAAACATCTTCCCGATGTGGGACTGGGTGCGTGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGTGCCTacac > 6:156063/1‑148 (MQ=37) tgTAGCGTTCGGTATCCGCGAAGAAAACATCTTCCCGATGTGGGACTGGGTGGGTGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGTGCCTACACCa > 1:335168/1‑149 (MQ=37) ttCGGTATCCGCGAAGAAAACATCTTCCCGATGTGGGACTGGGTGGGTGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGTGCCTACACCATGGAc > 4:366553/1‑147 (MQ=37) ttCGGTATCCGCGAAGAAAACATCTTCCCGATGTGGGACTGGGTGGGTGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGTGCCTACACCATGGAc > 6:192625/1‑147 (MQ=37) cGGTATCCGCGAAGAAAACATCTTCCCGATGTGGGACTGGGTGTGTGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGTGCCTACACCATGGACCAg < 1:49029/148‑1 (MQ=37) cgcgAAGAAAACATCTTCCCGATGTGGGACTGGGTGGGTGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGTGCCTACACCATGGACCAGCACTTc > 2:270690/1‑147 (MQ=37) aaaCATCTTCCCGATGTGGGACTGGGTGGGTGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGTGCCTACACCATGGACCAGCACTTCCAGACCGCAc > 5:296896/1‑149 (MQ=37) aCATCTTCCCGATGTGGGACTGGGTGGGTGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGcc > 5:158653/1‑89 (MQ=38) aTGTGGGACTGGGTGGGTGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGTGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGa > 7:10467/1‑148 (MQ=34) gggACTGGGTGGGTGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGTGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATg > 6:174443/1‑149 (MQ=34) ggCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGTGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGcc < 8:10467/149‑1 (MQ=32) gcTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGTGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCctg > 2:299702/1‑149 (MQ=32) cTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGTGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCgt < 6:296896/149‑1 (MQ=32) gtgGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGTGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCgtgt > 1:145271/1‑149 (MQ=32) tgGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGTGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCgtgt < 5:192625/148‑1 (MQ=32) ggTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGTGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCctgc > 3:277615/1‑138 (MQ=32) ggTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGTGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCctgc < 4:277615/138‑1 (MQ=32) ccggggCGATCGGCAGGCCGATGGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGTGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCGtgtg < 8:315535/144‑1 (MQ=11) ccagggCGATCGGCAGGCCGATGGCCCTGGCCATCGGCACGGCCAACTTCAAGGAACTGCTGTCCGGTGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGGGCTACTGGCCCTGCTGGGCGTGTgg > 7:315535/5‑149 (MQ=11) gggCGATCGGCAGGCCGATGGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGTGCCTACACCATGGACCAGCACTTCCAGACAGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCGTGTGGTAt > 2:229337/2‑149 (MQ=11) ggCGATCGGCAGGCCGATGGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGTGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCGTGTGGTATg > 7:200565/1‑149 (MQ=255) ggCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGTGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCGTGTGGTATg > 8:272589/1‑149 (MQ=32) ggCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGTGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCGTGTGGTATg < 3:366553/149‑1 (MQ=32) cATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGTGCCTACACCATGGACCTGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTg > 5:225136/1‑124 (MQ=17) cATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGTGCCTACACCATGGACCTGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTg < 6:225136/124‑1 (MQ=17) tCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGTGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCGTGTGGTATGGCAAc < 2:194045/149‑1 (MQ=32) agtGCCGATGGCCGGGGCGATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGTGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGc < 5:257722/128‑1 (MQ=11) agtGCCGATGGCCGGGGCGATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGTGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGc > 6:257722/3‑130 (MQ=11) gtGCCGATGGCCAGGGCGATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGTGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTgg < 6:238041/126‑1 (MQ=11) gtGCCGATGGCCAGGGCGATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGTGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTgg > 5:238041/2‑127 (MQ=11) tGCCGATGGCCAGGGCGATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGTGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCGTGTGGTATGGCAACTTCTgg > 4:126164/1‑149 (MQ=255) ccGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGTGCCTACACCATGGACCAGCACTTCCATACCGCACCGTTCGAAAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCGTGTGGTATGGCAACTTCTgg < 1:56589/147‑1 (MQ=17) ccGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGTGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCGTGTGGTATGGCAACTTCTgg < 5:156063/147‑1 (MQ=32) ccGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGTGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCGTGTGGTATGGCAACTTCTgg < 1:299702/147‑1 (MQ=32) cGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGTGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCGTGTGGTATGGCAACTTCTGGGAc < 1:270690/149‑1 (MQ=32) gATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGTGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTgg < 4:327449/122‑1 (MQ=32) | TACCGCCACTTCATTGCCGTATCCAGCAACAAGGCCGCTGCTGTAGCGTTCGGTATCCGCGAAGAAAACATCTTCCCGATGTGGGACTGGGTGGGTGGGCGCTACTCGCTGTGGTCGGCCATCGGCCTGCCGATCGCCCTGGCCATCGGCACTGCCAACTTCAAGGAACTGCTGTCCGGTGCCTACACCATGGACCAGCACTTCCAGACCGCACCGTTCGACAAGAACATGCCGGTGCTACTGGCCCTGCTGGGCGTGTGGTATGGCAACTTCTGGGAC > NC_002947/2033240‑2033518 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |