Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,068,151	A→T	7.5%	intergenic (‑96/+25)	plsC ← / ← PP_1845	1‑acyl‑sn‑glycerol‑3‑phosphate acyltransferase/enoyl‑CoA hydratase/isomerase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,068,151	0	A	T	7.5%	154.2 / 3.3	53	intergenic (‑96/+25)	plsC/PP_1845	1‑acyl‑sn‑glycerol‑3‑phosphate acyltransferase/enoyl‑CoA hydratase/isomerase family protein
Reads supporting (aligned to +/- strand):  ref base A (24/25);  new base T (2/2);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

GGCAAGAAAATGCAGCCCCAGCAGGAACATACGCAATGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGC‑CCTTTGCGCACTTTCAGCTTGGTGTTCTTGTGCGCCAGCATGTTCAGCTTCTTCAGCT  >  NC_002947/2068008‑2068288                                                                                                                                                 |                                                                                                                                           ggCAAGAAAATGCAGCCCCAGCAGGAACATACGCAATGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGccc                                                                                                                                       <  4:184753/149‑1 (MQ=255)  gCAAGAAAATGCAGCCCCAGCAGGAACATACGCAATGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCcg                                                                                                                                      <  3:224284/149‑1 (MQ=255)    aaGAAAATGCAGCCCCAGCAGGAACATACGCAATGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCcgc                                                                                                                                     >  8:230023/1‑148 (MQ=255)    aaGAAAATGCAGCCCCAGCAGGAACATACGCAATGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCcgc                                                                                                                                     >  4:77895/1‑148 (MQ=255)      gAAAATGCAGCCCCAGCAGGAACATACGCAATGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGa                                                                                                                                   >  4:221565/1‑148 (MQ=255)       aaaaTGCAGCCCCAGCAGGAACATACGCAATGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCT‑TTTCGCGGGCTTGCCCGCGaaa                                                                                                                                 <  1:155853/149‑1 (MQ=255)        aaaTGCAGCCCCAGCAGGAACATACGCAATGAATAAAGCATGGTACGACTCACACCAGACGGGCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGaaaa                                                                                                                                <  7:93874/149‑1 (MQ=255)         aaTGCAGCCCCAGCAGGAACATACGCAATGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCCTTTTCGCGGGCTTGCCCGCGaaag                                                                                                                                <  4:146177/149‑2 (MQ=255)         aaTGCAGCCCCAGCAGGAACATACGCAATGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAg                                                                                                                               <  6:164822/149‑1 (MQ=255)          aTGCAGCCCCAGCAGGAACATACGCAATGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAgg                                                                                                                              <  5:103696/149‑1 (MQ=255)          aTGCAGCCCCAGCAGGAACATACGCAATGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAgg                                                                                                                              <  7:230023/149‑1 (MQ=255)          aTGCAGCCCCAGCAGGAACATACGCAATGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAgg                                                                                                                              <  3:77895/149‑1 (MQ=255)                ccccAGCAGGAACATACGCAATGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGt                                                                                                                        >  5:50957/1‑149 (MQ=255)                               aCGCAATGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAatat                                                                                                         >  1:154362/1‑149 (MQ=255)                                 gCAATGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGc                                                                                                               >  6:61209/1‑141 (MQ=255)                                 gCAATGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGc                                                                                                               <  5:61209/141‑1 (MQ=255)                                 gCAATGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAtgc                                                                                                       >  6:254914/1‑149 (MQ=255)                                    aTGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGaaaa                                                                                                                                >  6:82868/1‑121 (MQ=255)                                    aTGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGaaaa                                                                                                                                <  5:82868/121‑1 (MQ=255)                                    aTGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATAtgctgc                                                                                                    <  6:160622/149‑1 (MQ=255)                                        aTAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGAt                                                                                                >  2:239620/1‑149 (MQ=255)                                              cATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGc                                                                                           <  7:218648/148‑1 (MQ=255)                                              cATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGc                                                                                           >  8:218648/1‑148 (MQ=255)                                              cATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCt                                                                                          >  7:312173/1‑149 (MQ=255)                                                  gTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGAGTGCACTGGCTGGGGCAAACCTTGCTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCACCTCGAt                                                                                      >  3:214164/1‑149 (MQ=255)                                                   tACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATc                                                                                     <  2:303666/149‑1 (MQ=255)                                                           acacCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGt                                                                              <  3:221565/148‑1 (MQ=255)                                                             acCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTc                                                                             >  1:19526/1‑147 (MQ=255)                                                             acCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCa                                                                           <  8:312173/149‑1 (MQ=255)                                                                                        gtgtGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGc                                                                                                               >  3:126587/1‑86 (MQ=255)                                                                                        gtgtGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGc                                                                                                               <  4:126587/86‑1 (MQ=255)                                                                                        gtgtGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGC‑CCTTTGCGCACtt                                               >  2:38108/1‑149 (MQ=255)                                                                                         tgtgCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGC‑CCTTTGCGCACttt                                              <  5:254914/149‑1 (MQ=255)                                                                                                  gctggGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGC‑CCTTTGCGCACTTTCAGCTTGgt                                     <  1:239620/149‑1 (MQ=255)                                                                                                  gctggGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCACGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGC‑CCTTTGCGCACTTTCAGCTTGgt                                     <  6:50957/149‑1 (MQ=255)                                                                                                       ggCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGC‑CCTTTGCGCACTTTCAGCTTGGTGTTc                                 <  5:55832/148‑1 (MQ=255)                                                                                                       ggCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGC‑CCTTTGCGCACTTTCAGCTTGGTGTTc                                 >  6:55832/1‑148 (MQ=255)                                                                                                        gCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGC‑CCTTTGCGCACTTTCAGCTTGGTGTTc                                 <  3:313425/147‑1 (MQ=255)                                                                                                        gCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGC‑CCTTTGCGCACTTTCAGCTTGGTGTTc                                 >  4:313425/1‑147 (MQ=255)                                                                                                        gCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGC‑CCTTTGCGCACTTTCAGCTTGGTGTTCtt                               >  7:133324/1‑149 (MQ=255)                                                                                                        gCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGC‑CCTTTGCGCACTTTCAGCTTGGTGTTCtt                               >  2:101894/1‑149 (MQ=255)                                                                                                        gCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGC‑CCTTTGCGCACTTTCAGCTTGGTGTTCtt                               >  8:252446/1‑149 (MQ=255)                                                                                                          aaaCCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGC‑CCTTTGCGCACTTTCAGCTTGGTGTTCTtgt                             <  4:227066/149‑1 (MQ=255)                                                                                                                    gCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGC‑CCTTTGCGCACTTTCAGCTTGGTGTTCTTGTGCGCCAGCAt                   >  4:305057/1‑149 (MQ=255)                                                                                                                       ggCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGC‑CCTTTGCGCACTTTCAGCTTGGTGTTCTTGTGCGCCAGCATGtt                >  1:124529/1‑149 (MQ=255)                                                                                                                           tGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGC‑CCTTTGCGCACTTTCAGCTTGGTGTTCtt                               <  7:305705/130‑1 (MQ=255)                                                                                                                           tGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGC‑CCTTTGCGCACTTTCAGCTTGGTGTTCtt                               >  8:305705/1‑130 (MQ=255)                                                                                                                                cGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGC‑CCTTTGCGCACTTTCAGCTTGGTGTTCTTGTGCGCCAGCATGTTCAGcttctt       <  2:154362/149‑1 (MQ=255)                                                                                                                                 ggCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGC‑CCTTTGCGCACTTTCAGCTTGGTGTTCTTGTGCGCCAGCATGTTCAGCttcttc      <  3:305057/149‑1 (MQ=255)                                                                                                                                  gCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGC‑CCTTTGCGCACTTTCAGCTTGGTGTTCTTGTGCGCCAGCATGTTCAGCTTCTTCa     >  6:122051/1‑149 (MQ=255)                                                                                                                                   ccc‑TTTCGCGGGCAATCCCGCGAAAAGGGCCCGGGCGGCTATCCAATATGCTGCTGATCCATGTCGATCGCCTTTTCCAGCGCCTCCAGCATCTCCTTTGCGCACTTTCAGCTTGGTGTTCTTGTGCGCCAGCATGTTCAGCTTCTTCa     <  1:101894/149‑1 (MQ=255)                                                                                                                                    ccttttCGCGGGCTTGCCCGCG‑AAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGC‑CCTTTGCGCACTTTCAGCTTGGTGTTCTTGTGCGCCAGCATGTTCAGCTTCTTCAGc   >  2:352525/4‑149 (MQ=255)                                                                                                                                      ttttCGCGGGCTTGCCCGCGAAAAGGGCACGTGCGGCTAGCCAATATGCTGATGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGC‑CCTTTGCGCACTTTCAGCTTGGTGTTCTTGTGCGCCAGCATGTTCAGCTTCTTCAGCt  >  6:107406/2‑149 (MQ=255)                                                                                                                                                 |                                                                                                                                           GGCAAGAAAATGCAGCCCCAGCAGGAACATACGCAATGAATAAAGCATGGTACGACTCACACCAGACGGTCGCGGGGCAGTGTACGGGTGTGCACTGGCTGGGGCAAACCTTGGTGCAGGCCTGTTACGGCCC‑TTTCGCGGGCAAGCCCGCGAAAAGGGCCCGTGCGGCTAGCCAATATGCTGCTGATCCAGCTCGATCGCCTTGTCCAGCGCCTCCAGCAGC‑CCTTTGCGCACTTTCAGCTTGGTGTTCTTGTGCGCCAGCATGTTCAGCTTCTTCAGCT  >  NC_002947/2068008‑2068288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑