Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,074,029 C→G 7.7% intergenic (‑41/+162) PP_1849 ← / ← ygaY hypothetical protein/transporter YgaY

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,074,0290CG 7.7% 156.8 / 2.8 51intergenic (‑41/+162)PP_1849/ygaYhypothetical protein/transporter YgaY
Reads supporting (aligned to +/- strand):  ref base C (23/24);  new base G (2/2);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

GGGTGAGGGGCTGCGCGCGGTCTTGAGTGTGAGCGATCCATTGCGATACTCGCCTTCACCTTGC‑AAGAAGTCGCCGGATTGCACCGTGAGTCTTGTCATAAAGTCATCCTGTCAGGGAAAGGGCATTTTCCAGCGCTTACTGAGCGCTGCCCCGGCAAGCAAGTCAACGGCATTGCGACGGAGGGTAGTGCGGTACTTTGTCGCCGTGGCCAATGGCCTCTTCGCGGGCGCTTCACAAACCTCAGGGCCGTGATACGTCTGTGGGAGCGGGCGTGCCCGCGAAGAATC  >  NC_002947/2073890‑2074177
                                                                                                                                            |                                                                                                                                                    
gggTGAGGGGCTGCGCGCGGTCTTGAGTGTGAGCGATCCATTGCGATACTCGCCTTCACCTTGC‑AAGAAGTCGCCGGATTGCACCGTGAGTCTTGTCATAAAGTCATCCTGTCAGGGAAAGGGCATTTTCCAGCGCTTACTGAGCGCTg                                                                                                                                             >  3:224928/1‑149 (MQ=255)
gggTGAGGGGCTGCGCGCGGGCTTGAGTGTGAGCGATCCATTGCGATACTCGCCTTCACCTTGC‑AAGAAGTCGCCGGATTGCACCGTGAGTCTTGTCATAAAGTCATCCTGTCAGGGAAAGGGCATTTTCCAGCGCTCAGTAAGCGCTg                                                                                                                                             <  5:32360/149‑1 (MQ=255)
     atgggCTGCGCGCGGTCTTGAGTGTGAGCGATCGATTGCGTTACTCGTCTTCTGCTTGC‑ATGATGTGGCCGGATTGCAGCTTGTGTGTTGTCATAAAGTCTTGGTGTCAGGGAAAGGGCATTTTCCAGCGCTTACTGAGCGCTGCCCCg                                                                                                                                        <  6:94542/147‑1 (MQ=255)
     aGGGGCTGCGCGCGGTCTTGAGTGTGAGCGATCCATTGCGATACTCGCCTTCACCTTGC‑AAGAAGTCGCCGGATTGCACCGTGAGTCTTGTCATAAAGTCATCCTGTCAGGGAAAGGGCATTTTCCAGCGCTTACTGAGCGCTGcccc                                                                                                                                         <  6:264188/148‑1 (MQ=255)
         gCTGCGCGCGGTCTTGAGTGTGAGCGATCCATTGCGATACTCGCCTTCACCTTGC‑AAGAAGTCGCCGGATTGCACCGTGAGTCTTGTCATAAAGTCATCCTGTCAGGGAAAGGGCATTTTCCAGCGCTTACTGAGCGCTGCCCCGgcaa                                                                                                                                    <  3:226819/149‑1 (MQ=255)
                    tCTTGAGTGTGAGCGATCCATTGCGATACTCGCCTTCACCTTGC‑AAGAAGTCGCCGGATTGCACCGTGAGTCTTGTCATAAAGTCATCCTGTCAGGGAAAGGGCATTTTCCAGCGCTTACTGAGCGCTGCCCCGGCAAGCAAGTCAACg                                                                                                                         <  1:344737/149‑1 (MQ=255)
                    tCTTGAGTGTGAGCGATCCATTGCGATACTCGCCTTCACCTTGC‑AAGAAGTCGCCGGATTGCACCGTGAGTCTTGTCATAAAGTCATCCTGTCAGGGAAAGGGCATTTTCCAGCGCTTACTGAGCGCTGCCCCGGCAAGCAAGTCAACg                                                                                                                         <  1:60544/149‑1 (MQ=255)
                               aGCGATCCATTGCGATACTCGCCTTCACCTTGC‑AAGAAGTCGCCGGATTGCACCGTGAGTCTTGTCATAAAGTCATCCTGTCAGGGAAAGGGCATTTTCCAGCGCTTACTGAGCGCTGCCCCGGCAAGCAAGTCAACGGCATTGCGAc                                                                                                               <  4:224928/148‑1 (MQ=255)
                                 cGATCCATTGCGATACTCGCCTTCACCTTGCGAAGAAGTCGCCGGATTGCACCGTGAGTCTTGTCATAAAGTCATCCTGTCAGGGAAAGGGCATTTTCCAGCGCTTACTGAGCGCTGCCCCGGCAAGCAAGTCAACGGCATTGCGACg                                                                                                              >  1:226928/1‑148 (MQ=255)
                                      cATTGCGATACTCGCCTTCACCTTGC‑AAGAAGTCGCCGGATTGCACCGTGAGTCTTGTCATAAAGTCATCCTGTCAGGGAAAGGGCATTTTCCAGCGCTTACTGAGCGCTGCCCCGGCAAGCAAGTCAACGGCATTGCGACGGAGGGTa                                                                                                       <  2:74460/149‑1 (MQ=255)
                                       aTTGCGATACTCGCCTTCACCTTGC‑AAGAAGTCGCCGGATTGCACCGTGAGTCTTGTCATAAAGTCATCCTGTCAGGGAAAGGGCATTTTCCAGCGCTTACTGAGCGCTGCCCCGGCAAGCAAGTCAACGGCATTGCGACGGAGGGTAg                                                                                                      >  6:363692/1‑149 (MQ=255)
                                       aTTGCGATACTCGCCTTCACCTTGC‑AAGAAGCCGCCGGATTGCACCGTGAGTCTTGTCATAAAGTCATCCTGTCAGGGAAAGGGCATTTTCCAGCGCTTACTGAGCGCTGCCCCGgc                                                                                                                                      >  8:25083/1‑117 (MQ=255)
                                       aTTGCGATACTCGCCTTCACCTTGC‑AAGAAGCCGCCGGATTGCACCGTGAGTCTTGTCATAAAGTCATCCTGTCAGGGAAAGGGCATTTTCCAGCGCTTACTGAGCGCTGCCCCGgc                                                                                                                                      <  7:25083/117‑1 (MQ=255)
                                           cGATACTCGCCTTCACCTTGC‑AAGAAGTCGCCGGATTGCACCGTGAGTCTTGTCATAAAGTCATCCTGTCAGGGAAAGGGCATTTTCCAGCGCTTACTGAGCGCTGCCCCGGCAAGCAAGTCAACGGCATTGCGACGGAg                                                                                                           <  8:166357/140‑1 (MQ=255)
                                           cGATACTCGCCTTCACCTTGC‑AAGAAGTCGCCGGATTGCACCGTGAGTCTTGTCATAAAGTCATCCTGTCAGGGAAAGGGCATTTTCCAGCGCTTACTGAGCGCTGCCCCGGCAAGCAAGTCAACGGCATTGCGACGGAg                                                                                                           >  7:166357/1‑140 (MQ=255)
                                              tACTCGCCTTCACCTTGC‑AAGAAGTCGCCGGATTGCACCGTGAGTCTTGTCATAAAGTCATCCTGTCAGGGAAAGGGCATTTTCCAGCGCTTACTGAGCGCTGCCCCGGCAAGCAAGTCAACGGCATTGCGACGGAGGGTAGTGCGGTa                                                                                               <  8:114568/149‑1 (MQ=255)
                                                 tCGCCTTCACCTTGC‑AAGAAGTCGCCGGATTGCACCGTGAGTCTTGTCATAAAGTCATCCTGTCAGGGAAAGGGCATTTTCCAGCGCTTACTGAGCGCTGCCCCGGCAAGCAAGTCAACGGCATTGCGACGGAGGGTAGTGCGGTACtt                                                                                            >  4:123970/1‑149 (MQ=255)
                                                 tCGCCTTCACCTTGC‑AAGAAGTCGCCGGATTGCACCGTGAGTCTTGTCATAAAGTCATCCTGTCAGGGAAAGGGCATTTTCCAGCGCTTACTGAGCGCTGCCCCGGCAAGCAAGTCAACGGCATTGCGACGGAGGGTAGTGCGGTACtt                                                                                            >  6:75842/1‑149 (MQ=255)
                                                          ccTTGC‑AAGAAGTCGCCGGATTGCACCGTGAGTCTTGTCATAAAGTCATCCTGTCAGGGAAAGGGCATTTTCCAGCGCTTACTGAGCGCTGCCCCGGCAAGCAAGTCAACGGCATTGCGACGGAGGGTAGTGCGGTACTTTGTCGCCGt                                                                                   >  7:304651/1‑149 (MQ=255)
                                                           cTTGC‑AAGAAGTCGCCGGATTGCACCGTGAGTCTTGTCATAAAGTCATCCTGTCAGGGAAAGGGCATTTTCCAGCGCTTACTGAGCGCTGCCCCGGCAAGCAAGTCAACGGCATTGCGACGGAGGGTAGTGCGGTACTTTGTCGCCGTg                                                                                  >  4:73607/1‑149 (MQ=255)
                                                              gC‑AAGAAGTCGCCGGATTGCACCGTGAGTCTTGTCATAAAGTCATCCTGTCAGGGAAAGGGCATTTTCCAGCGCTTACTGAGCGCTGCCCCGGCAAGCAAGTCAACGGCAtt                                                                                                                    >  5:72740/1‑112 (MQ=255)
                                                              gC‑AAGAAGTCGCCGGATTGCACCGTGAGTCTTGTCATAAAGTCATCCTGTCAGGGAAAGGGCATTTTCCAGCGCTTACTGAGCGCTGCCCCGGCAAGCAAGTCAACGGCAtt                                                                                                                    <  6:72740/112‑1 (MQ=255)
                                                                         gCCGGATTGCACCGTGAGTCTTGTCATAAAGTCATCCTGTCAGGGAAAGGGCATTTTCCAGCGCTTACTGAGCGCTGCCCCGGCAAGCAAGTCAACGGCATTGCGACGGAGGGTAGTGCGGTACTTTGTCGCCGTGGCCAATGGCctct                                                                     >  7:147833/1‑149 (MQ=255)
                                                                         gCCGGATTGCACAGTGAGTCTTGTCATAAAGTCATCCTGTCAGGGAAAGGGCATTTTCCAGCGCTTACTGAGCGCTGCCCCGGCAAGCAAGTCAACGGCATTGCGACGGAGGGTAGTGCGGTACTTTGTCGCCGTGGCCAATGGCctct                                                                     >  2:133511/1‑149 (MQ=255)
                                                                               ttGCACCGTGAGTCTTGTCATAAAGTCATCCTGTCAGGGAAAGGGCATTTTCCAGCGCTTACTGAGCGCTGCCCCGGCAAGCAAGTCAACGGCATTGCGACGGAGGGTAGTGCGGTACTTTGTCGCCGTGGCCAATGGCCTCTTCGCgg                                                               <  3:73607/149‑1 (MQ=255)
                                                                                  cACCGTGAGTCTTGTCATAAAGTCATCCTGTCAGGGAAAGGGCATTTTCCAGCGCTTACTGAGCGCTGCCCCGGCAAGCAAGTCAACGGCATTg                                                                                                                   >  3:217491/1‑94 (MQ=255)
                                                                                  cACCGTGAGTCTTGTCATAAAGTCATCCTGTCAGGGAAAGGGCATTTTCCAGCGCTTACTGAGCGCTGCCCCGGCAAGCAAGTCAACGGCATTg                                                                                                                   <  4:217491/94‑1 (MQ=255)
                                                                                   aCCGTGAGTCTTGTCATAAAGTCATCCTGTCAGGGAAAGGGCATTTTCCAGCGCTTACTGAGCGCTGCCCCGGCAAGCAAGTCAACGGCATTGCGACGGAGGGTAGTGCGGTACTTTGTCGCCGTGGCCAATGGCCTCTTCGCGGgcgc                                                           >  4:165962/1‑149 (MQ=255)
                                                                                        gAGTCTTGTCATAAAGTCATCCTGTCAGGGAAAGGGCATTTTCCAGCGCTTACTGAGCGCTGCCCCGGCAAGCAAGTCAACgg                                                                                                                        >  6:339127/1‑83 (MQ=255)
                                                                                        gAGTCTTGTCATAAAGTCATCCTGTCAGGGAAAGGGCATTTTCCAGCGCTTACTGAGCGCTGCCCCGGCAAGCAAGTCAACgg                                                                                                                        <  5:339127/83‑1 (MQ=255)
                                                                                         aGTCTTGTCATAAAGTCATCCTGTCAGGGAAAGGGCATTTTCCAGCGCTTACTGAGCGCTGCCCCGGCAAGCAAGTCAACGGCATTGCGACGGAGGGTAGTGCGGTACTTTGTCGCCGTGGCCAATGGCCTCTTCGCGGGCGCTTcac                                                      <  7:187916/148‑1 (MQ=255)
                                                                                         aGTCTTGTCATAAAGTCATCCTGTCAGGGAAAGGGCATTTTCCAGCGCTTACTGAGCGCTGCCCCGGCAAGCAAGTCAACGGCATTGCGACGGAGGGTAGTGCGGTACTTTGTCGCCGTGGCCAATGGCCTCTTCGCGGGCGCTTcac                                                      <  5:363692/148‑1 (MQ=255)
                                                                                            cTTGTCATAAAGTCATCCTGTCAGGGAAAGGGCATTTTCCAGCGCTTACTGAGCGCTGCCCCGGCAAGCAAGTCAACGGCATTGCGACGGAGGGTAGTGCGGTACTTTGTCGCCGTGGCCAATGGCCTCTTCGCGGGCGCTTCACAAAc                                                  >  5:300228/1‑149 (MQ=255)
                                                                                                  aTAAAGTCATCCTGTCAGGGAAAGGGCATTTTCCAGCGCTTACTGAGCGCTGCCCCGGCAAGCAAGTCAACGGCATTGCGACGGAGGGTAGTGCGGTACTTTGTCGCCGTGGCCAATGGCCTCTTCGCGGGCGCTTCACAAACCTCAgg                                            >  1:207206/1‑149 (MQ=255)
                                                                                                       gTCATCGTGTCAGGGAAATGGCATTTTCCAGCGCTTACTGAGCGCTGCCCCGGCAAGCAAGTCAACGGCATTGCGACGGAGGGTAGTGCGGTACTTTGTCGCCGTGGCCAATGGCCTCTTCGCGGGCGCTTCACAAACCTCAGGGCCGt                                       <  4:348625/149‑1 (MQ=255)
                                                                                                             cTGTCAGTGAAAGGGCATTTTCCAGCGCTTACTGAGCGCTGCCCCGGCAAGCAAGTCAACGGCATTGCGACGGAGGGTAGTGCGGTACTTTGTCGCCGTGGCCAATGGCCTCTTCGCGGTCTATTCACAAACCTCAGGGCCGTGATAc                                  <  7:88535/148‑1 (MQ=255)
                                                                                                             cTGTCAGGGAAAGGGCATTTTCCAGCGCTTACTGAGCGCTGCCCCGGCAAGCAAGTCAACGGCATTGCGACGGAGGGTAGTGCGGTACTTTGTCGCCGTGGCCAATGGCCTCTTCGCGGGCGCTTCACAAACCTCAGGGCCGTGATACg                                 <  2:226928/149‑1 (MQ=255)
                                                                                                              tGTCAGGGAAAGGGCATTTTCCAGCGCTTACTGAGCGCTGCCCCGGCAAGCAAGTCAACGGCATTGCGACGGAGGGTa                                                                                                       >  4:130596/1‑78 (MQ=255)
                                                                                                              tGTCAGGGAAAGGGCATTTTCCAGCGCTTACTGAGCGCTGCCCCGGCAAGCAAGTCAACGGCATTGCGACGGAGGGTAGTGCGGTACTTTGTCGCCGTGGCCAATGGCCTCTTCGCGGGCGCTTCACAAACCTCAGGGCCGTGATACGt                                >  2:317990/1‑149 (MQ=255)
                                                                                                                     gAAAGGGCATTTTCCAGCGCTTACTGAGCGCTGCCCCGGCAAGCAAGTCAACGGCATTGCGACGGAGGGTAGTGCGGTACTTTGTCGCCGTGGCCAATGGCCTCTTCGCGGGCGCTTCACAAACCTCAGGGCCGTGATACGTCTTTgg                          <  1:133511/148‑1 (MQ=255)
                                                                                                                      aaaGGGCATTTTCCAGCGCTTACTGAGCGCTGCCCCGGCAAGCAAGTCAACGGCATTGCGACGGAGGGTAGTGCGGTACTTTGTCGCCGTGGCCAATGGCCTCTTCGCGGGCGCTTCACAAACCTCAGGGCCGTGATACGTCTGTgg                          >  4:370174/1‑147 (MQ=255)
                                                                                                                          ggCATTTTCCAGCGCTTACTGAGCGCTGCCCCGGCAAGCAAGTCAACGGCATTGCGACGGAGGGTAGTGCGGTACTTTGTCGCCGTGGCCAATGGCCTCTTCGCGGGCGCTTCACAAACCTCAGGGCCGTGATACGTCTGTGGGAGCgg                    <  6:3089/149‑1 (MQ=255)
                                                                                                                          ggCATTTTCCAGCGCTTACTGAGCGCTGCCCCGGCAAGCAAGTCAACGGCATTGCGACGGAGGGTAGTGCGGTACTTTGTCGCCGTGGCCAATGGCCTCTTCGCGGGCGCTTCACAAACCTCAGGGCCGTGATACGTCTGTGGGAGCgg                    >  5:3089/1‑149 (MQ=255)
                                                                                                                                 ggcAGCGCTCAGTAAGCGCTGCCCCGGCAAGCAAGTCAACGGCATTGCGACGGAGGGTAGTGCGGTACTTTGTCGCCGTGGCCAATGGCCTCTTCGCGGGCGCTTCACAAACCTCAGGGCCGTGATACGTCTGTGGGAGCGGGCGTg               >  6:223478/3‑147 (MQ=255)
                                                                                                                                 ggcAGCGCTCAGTAAGCGCTGCCCCGGCAAGCAAGTCAACGGCATTGCGACGGAGGGTAGTGCGGTACTTTGTCGCCGTGGCCAATGGCCTCTTCGCGGGCGCTTCACAAACCTCAGGGCCGTGATACGTCTGTGGGAGCGGGCGTg               <  5:223478/145‑1 (MQ=255)
                                                                                                                                  gcAGCGCTCAGTAAGCGCTGCCCCGGCAAGCAAGTCAACGGCATTGCGACGGAGGGTAGTGCGGTACTTTGTCGCCGTGGCCAATGGCCTCTTCGCGGGCGCTTCACAAACCTCAGGGCCGTGATACGTCTGTGGGAGCGGGCGTGccc            >  2:45261/2‑149 (MQ=255)
                                                                                                                                  ccAGCGCTTACTGAGCGCTGCCCCGGCAAGCAAGTCAACGGCATTGCGACGGAGGGTAGTGCGGTACTTTGTCGCCGTGGCCAATGGCCTCTTCGCGGGCGCTTCACAAACCTCAGGGCCGTGATACGTCTGTGGGAGCGGGCGTGccc            >  6:142345/1‑149 (MQ=255)
                                                                                                                                  ccAGCGCTTACTGAGCGCTGCCCCGGCAAGCAAGTCAACGGCATTGCGACGGAGGGTAGTGCGGTACTTTGTCGCCGTGGCCAATGGCCTCTTCGCGGGCGCTTCACAAACCTCAGGGCCGTGATACGTCTGTGGGAGCGGGCGTGcc             <  6:300228/148‑1 (MQ=255)
                                                                                                                                    aGCGCTTACTGAGCGCTGCCCCGGCAAGCAAGTCAACGGCATTGCGACGGAGGGTAGTGCGGTACTTTGTCGCCGTGGCCAATGGCCTCTTCGCGGGCGCTTc                                                        >  3:345312/1‑103 (MQ=255)
                                                                                                                                    aGCGCTTACTGAGCGCTGCCCCGGCAAGCAAGTCAACGGCATTGCGACGGAGGGTAGTGCGGTACTTTGTCGCCGTGGCCAATGGCCTCTTCGCGGGCGCTTc                                                        <  4:345312/103‑1 (MQ=255)
                                                                                                                                           aCTGAGCGCTGCCCCGGCAAGCAAGTCAACGGCATTGCGACGGAGGGTAGTGCGGTACTTTGTCGCCGTGGCCAATGGCCTCTTCGCGGGCGCTTCACAAACCTCAGGGCCGTGATACGTCTGTGGGAGCGGGCGTGCCCGCgaaga     <  8:147833/147‑1 (MQ=255)
                                                                                                                                            cTGAGCGCTGCCCCGGCAAGCAAGTCAACGGCATTGCGACGGAGGGTAGTGCGGTACTTTGTCGCCGTGGCCAATGGCCTCTTCGCGGGCGCTTCACAAACCTCAGGGCCGTGATACGTCTGTGGGAGCGGGCGTGCCCGCGAAGAATc  <  3:165962/149‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                                    
GGGTGAGGGGCTGCGCGCGGTCTTGAGTGTGAGCGATCCATTGCGATACTCGCCTTCACCTTGC‑AAGAAGTCGCCGGATTGCACCGTGAGTCTTGTCATAAAGTCATCCTGTCAGGGAAAGGGCATTTTCCAGCGCTTACTGAGCGCTGCCCCGGCAAGCAAGTCAACGGCATTGCGACGGAGGGTAGTGCGGTACTTTGTCGCCGTGGCCAATGGCCTCTTCGCGGGCGCTTCACAAACCTCAGGGCCGTGATACGTCTGTGGGAGCGGGCGTGCCCGCGAAGAATC  >  NC_002947/2073890‑2074177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑