Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,077,196 G→A 8.2% intergenic (‑38/‑81) PP_1852 ← / → PP_1853 enoyl‑ACP reductase/LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,077,1960GA 8.2% 135.0 / 2.4 49intergenic (‑38/‑81)PP_1852/PP_1853enoyl‑ACP reductase/LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (21/23);  new base A (2/2);  total (24/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.35e-01

CGCGCCTTCGCGGGCCAGGCGCCTTACGATAGCTGCGCCAATGCCTCGGGAACCGCCCTGAACCAGGGCCACTTTGCCTTCGAGTGTAAGTTGCTTGGACATGCTGTTCTCCTGCTGGAAGCCAGGCCGAAATGCCTTGGATGGGCACAGTATCGGCGCTCGATTGCCTACTGATAAGATGGTAATCACTATCAGCAGAGTAAACCTTTGGTTGCTAATCATGGCCATGGAATCATTCAGCGCGTTGGAATGCTTCATCCGCAGTGCCGAGGTCGGCAGTT  >  NC_002947/2077057‑2077337
                                                                                                                                           |                                                                                                                                             
cgcgcCTTCGCGGGCCAGGCGCCTTACGATAGCTGCGCCAATGCCTCGGGAACCGCCCTGAACCAGGGCCACTTTGCCTTCGAGTGTAAGTTGCTTGGACATGCTGTTCTCCTGCTGGAAGCCAGGCCGAAATGCCTTGGATGGGcaca                                                                                                                                      <  7:43200/149‑1 (MQ=255)
     cTTCGCGGGCCAGGCGCCTTACGATAGCTGCGCCAATGCCTCGGGAACCGCCCTGAACCAGGGCCACTTTGCCTTCGAGTGTAAGTTGCTTGGACATGCTGTTCTCCTGCTGGAAGCCAGGCCGAAATGCCTTGGATGGGCACAGTATc                                                                                                                                 >  3:180160/1‑149 (MQ=255)
        cgcgGGCCAGGCGCCTTACGATAGCTGCGCCAATTCCTCGGGAACCGCCCTGAACCAGGGCCACTTTGCCTTCGAGTGTAAGTTGCTTGGACATGCTGTTCTCCTGCTGGAAGCCAGGCCGAAATGCCTTGGATGGGCACAGTATCg                                                                                                                                <  5:253421/147‑1 (MQ=255)
        cgcgGGCCAGGCGCCTTACGATAGCTGCGCCAATGCCTCGGGAACCGCCCTGAACCAGGGCCACTTTGCCTTCGAGTGTAAGTTGCTTGGACATGCTGTTCTCCTGCTGGAAGCCAGGCCGAAATGCCTTGGATGGGCACAGTATCgg                                                                                                                               <  1:5450/148‑1 (MQ=255)
            ggCCAGGCGCCTTACGATAGCTGCGCCAATGCCTCGGGAACCGCCCTGAACCAGGGCCACTTTGCCTTCGAGTGTAAGTTGCTTGGACATGCTGTTCTCCTGCTGGAAGCCAGGCCGAAATGCCTTGGATGGGCACAGTATCGGCGCTc                                                                                                                          <  4:359593/149‑1 (MQ=255)
            ggCCAGGCGCCTTACGATAGCTGCGCCAATGCCTCGGGAACCGCCCTGAACCAGGGCCACTTTGCCTTCGAGTGTAAGTTGCTTGGACATGCTGTTCTCCTGCTGGAAGCCAGGCCGAAATGCCTTGGATGGGCACAGTATCGGCGCTc                                                                                                                          <  4:180160/149‑1 (MQ=255)
             gCCAGGCGCCTTACGATAGCTGCGCCAATGCCTCGGGAACCGCCCTGAACCAGGGCCACTTTGCCTTCGAGTGTAAGTTGCTTGGACATGCTGTTCTCCTGCTGGAAGCCAGGCCGAAATGCCTTGGATGGGCACAGTATCGGCGCTCg                                                                                                                         >  7:346014/1‑149 (MQ=255)
                aGGCGCCTTACGATAGCTGCGCCAATGCCTCGGGAACCGCCCTGAACCAGGGCCACTTTGCCTTCGAGTGTAAGTTGCTTGGACATGCTGTTCTCCTGCTGGAAGCCAGGCCGAAATGCCTTGGATGGGCACAGTATCGGCGCTCGAtt                                                                                                                      <  3:262469/149‑1 (MQ=255)
                 ggCGCCTTACGATAGCTGCGCCAATGCCTCGGGAACCGCCCTGAACCAGGGCCACTTTGCCTTCGAGTGTAAGTTGCTTGGACATGCTGTTCTCCTGCTGGAAGCCAGGCCGAAATGCCTTGGATGGGCACAGTATCGGCGCTCGATTg                                                                                                                     >  6:158531/1‑149 (MQ=255)
                  gcgcCTTACGATAGCTGCGCCAATGCCTCGGGAACCGCCCTGAACCAGGGCCACTTTGCCTTCGAGTGTAAGTTGCTTGGACATGCTGTTCTCCTGCTGGAAGCCAGGCCGAAATGCCTTGAATGGGCACAGTATCGGCGCTCGATTGc                                                                                                                    >  5:167555/1‑149 (MQ=255)
                  gcgcCTTACGATAGCTGCGCCAATGCCTCGGGAACCGCCCTGAACCAGGGCCACTTTGCCTTCGAGTGTAAGTTGCTTGGACATGCTGTTCTCCTGCTGGAAGCCAGGCCGAAATGCCTTGAATGGGCACAGTATCGGCGCTCGATTGc                                                                                                                    >  5:173028/1‑149 (MQ=255)
                      cTTACGATAGCTGCGCCAATGCCTCGGGAACCGCCCTGAACCAGGGCCACTTTGCCTTCGAGTGTAAGTTGCTTGGACATGCTGTTCTCCTGCTGGAAGCCAGGCCGAAATGCCTTGGATGGGCACAGTATCGGCGCTCGATTGCCTAc                                                                                                                <  8:346014/149‑1 (MQ=255)
                               gCTGCGCCAATGCCTCGGGAACCGCCCTGAACCAGGGCCACTTTGCCTTCGAGTGTAAGTTGCTTGGACATGCTGTTCTCCTGCTGGAAGCCAGGCCGAAATGCCTTGAATGGGCACAGTATCGGCGCTCGATTGCCTACTGATAAGa                                                                                                        <  6:173028/148‑1 (MQ=255)
                               gCTGCGCCAATGCCTCGGGAACCGCCCTGAACCAGGGCCACTTTGCCTTCGAGTGTAAGTTGCTTGGACATGCTGTTCTCCTGCTGGAAGCCAGGCCGAAATGCCTTGAATGGGCACAGTATCGGCGCTCGATTGCCTACTGATAAGa                                                                                                        <  6:167555/148‑1 (MQ=255)
                                      cAATGCCTCGGGAACCGCCCTGAACCAGGGCCACTTTGCCTTCGAGTGTAAGTTGCTTGGACATGCTGTTCTCCTGCTGGAAGCCAGGCCGAAATGCCTTGGATGGGCACAGTATCGGCGCTCGATTGCCTACTGATAAGATGGTAAt                                                                                                 >  2:196436/1‑148 (MQ=255)
                                       aaTGCCTCGGGAACCGCCCTGAACCAGGGCCACTTTGCCTTCGAGTGTAAGTTGCTTGGACATGCTGTTCTCCTGCTGGAAGCCAGGCCGAAATGCCTTGGATGGGCACAGTATCGGCGCTCGATTGCCTACTGATAAGATGGTAATCa                                                                                               <  5:158531/149‑1 (MQ=255)
                                            cTCGGGAACCGCCCTGAACCAGGGCCACTTTGCCTTCGAGTGTAAGTTGCTTGGACATGCTGTTCTCCTGCTGGAAGCCAGGCCGAAATGCCTTGGATGGGCACAGTATCGGCGCTCGATTGCCTACTGATAAGATGGTAATCACTAt                                                                                           >  6:378758/1‑148 (MQ=255)
                                             tCGGGAACCGCCCTGAACCAGGGCCACTTTGCCTTCGAGTGTAAGTTGCTTGGACATGCTGTTCTCCTGCTGGAAGCCAGGCCGAAATGCCTTGGATggg                                                                                                                                          <  2:370337/100‑1 (MQ=255)
                                             tCGGGAACCGCCCTGAACCAGGGCCACTTTGCCTTCGAGTGTAAGTTGCTTGGACATGCTGTTCTCCTGCTGGAAGCCAGGCCGAAATGCCTTGGATggg                                                                                                                                          >  1:370337/1‑100 (MQ=255)
                                                 ctaCCGCCCTGAACCAGGGCCAATTTGCCTTCGAGTGTAAGTTGCTTGGACATGCTGTTCTCCTGCTGGAAGCCAGGCCGAAATGCCTTGGATGGGCACAGTATCGGCGCTCGATTGCCTACTTACAAGATGGTAATCACTATcagca                                                                                      <  7:235396/146‑1 (MQ=255)
                                                   aCCGCCCTGAACCAGGGCCACTTTGCCTTCGAGTGTAAGTTGCTTGGACATGCTGTTCTCCTGCTGGAAGCCAGGCCGAAATGCCTTGGATGGGCACAGTATCGGCGCTCGATTGCCTACTGATAAGATGGTAATCACTATCAGCagag                                                                                   >  7:22697/1‑149 (MQ=255)
                                                    ccGCCCTGAACCAGGGCCACTTTGCCTTCGAGTGTAAGTTGCTTGGACATGCTGTTCTCCTGCTGGAAGCCAGGCCGAAATGCCTTGGATGGGCACAGTATCGGCGCTCGATTGCCTACTGATAAGATGGTAATCACTAtcatca                                                                                      >  8:235396/1‑145 (MQ=255)
                                                     cGCCCTGAACCAGGGCCACTTTGCCTTCGAGTGTAAGTTGCTTGGACATGCTGTTCTCCTGCTGGAAGCCAGGCCGAAATGCCTTGGATGGGCACAGTATCGGCGCTCGATTGc                                                                                                                    >  2:211084/1‑114 (MQ=255)
                                                     cGCCCTGAACCAGGGCCACTTTGCCTTCGAGTGTAAGTTGCTTGGACATGCTGTTCTCCTGCTGGAAGCCAGGCCGAAATGCCTTCGATGGGCACAGTATCGGCGCTCGATTGc                                                                                                                    <  1:211084/114‑1 (MQ=255)
                                                                aGGGCCACTTTGCCTTCGAGTGTAAGTTGCTTGGACATGCTGTTCTCCTGCTGGAAGCCAGGCCGAAATGCCTTGGATGGGCACAGTATCGGCGCTCGATTGCCTACTGATAAGATGGTAATCACTATCAGCAGAGTAAACCTTTGGtt                                                                      <  4:22493/149‑1 (MQ=255)
                                                                      aCTTTGCCTTCGAGTGTAAGTTGCTTGGACATGCTGTTCTCCTGCTGGAAGCCAGGCCGAAATGCCTTGGATGGGCACAGTATCGGCGCTCGATTGCCTAc                                                                                                                >  5:113725/1‑101 (MQ=255)
                                                                      aCTTTGCCTTCGAGTGTAAGTTGCTTGGACATGCTGTTCTCCTGCTGGAAGCCAGGCCGAAATGCCTTGGATGGGCACAGTATCGGCGCTCGATTGCCTAc                                                                                                                <  6:113725/101‑1 (MQ=255)
                                                                             cTTCGAGTGTAAGTTGCTTGGACATGCTGTTCTCCTGCTGGAAGCCAGGCCGAAATGCCTTGGATGGGCACAGTATCGGCGCTCGATTGCCTACTGATAAGATGGTAATCACTATCAGCAGAGTAAACCTTTGGTTGCTAATCATGGc                                                          >  6:349062/1‑148 (MQ=255)
                                                                                 gAGTGTAAGTTGCTTGGACATGCTGTTCTCCTGCTGGAAGCCAGGCCGAAATGCCTTGGATGGGCACAGTATCGGCGCTCGATTGCCTACTGATAAGATGGTAATCACTATCAGCAGAGTAAACCTTTGGTTGCTAATCATGGCCATg                                                      >  4:138669/1‑148 (MQ=255)
                                                                                   gtgtAAGTTGCTTGGACATGCTGTTCTCCTGCTGGAAGCCAGGCCGAAATGCCTTGGATGGGCACAGTATCGGCGCTCGATTGCCTACTGATAAGATGGTAATCACTATCAGCAGAGTAAACCTTTGGTTGCTAATCATGGCCATGGa                                                    >  8:46136/1‑148 (MQ=255)
                                                                                    tgtAAGTTGCTTGGACATGCTGTTCTCCTGCTGGAAGCCAGGCCGAAATGCCTTGGATGGGCACAGTATCGGCGCTCGATTGCCTACTGATAAGATGGTAATCACTATCAGCAGAGTAAACCTTTGGTTGCTAATCATGGCCATGGAAt                                                  >  2:182657/1‑149 (MQ=255)
                                                                                                 gACATGCTGTTCTCCTGCTGGAAGCCAGGCCGAAATGCCTTGGATGGGCACAGTATCGGCGCTCGATTGCCTACTGATAAGATGGTAATCACTATCAGCAGAGTAAACCTTTGGTTGCTAATCATGGCCATGGAATCATTCAGCGCGtt                                     >  8:285755/1‑149 (MQ=255)
                                                                                                     tGCTGTTCTCCTGCTGGAAGCCAGGCCGAAATGCCTTGGATGGGCACAGTATCGGCGCTCGATTGCCTACTGATAAGATGGTAATCACTATCAGCAGAGTAAACCTTTGGTTGCTAATCATGGCCATGGAATCATTCAGCGCGTTGGaa                                 <  5:378758/149‑1 (MQ=255)
                                                                                                        tGTTCTCCTGCTGGAAGCCAGGCCGAAATGCCTTGGATGGGCACAGTATCGGCGCTCGATTGCCTACTGATAAGATGGTAATCACTATCAGCAGAGTAAACCTTTGGTTGCTAATCATGGCCATGGAATCAtt                                              <  7:212995/133‑1 (MQ=255)
                                                                                                        tGTTCTCCTGCTGGAAGCCAGGCCGAAATGCCTTGGATGGGCACAGTATCGGCGCTCGATTGCCTACTGATAAGATGGTAATCACTATCAGCAGAGTAAACCTTTGGTTGCTAATCATGGCCATGGAATCAtt                                              >  8:212995/1‑133 (MQ=255)
                                                                                                          ttCTCCTGCTGGAAGCCAGGCCGAAATGCCTTGGATGGGCACAGTATCGGCGCTCGATTGCCTACTGATAAGATGGTAATCACTATCAGCAGAGTAAACCTTTGGTTGCTAATCATGGCCATGGAATCATTCAGCGCGTTg                                    <  4:181822/141‑1 (MQ=255)
                                                                                                          ttCTCCTGCTGGAAGCCAGGCCGAAATGCCTTGGATGGGCACAGTATCGGCGCTCGATTGCCTACTGATAAGATGGTAATCACTATCAGCAGAGTAAACCTTTGGTTGCTAATCATGGCCATGGAATCATTCAGCGCGTTg                                    >  3:181822/1‑141 (MQ=255)
                                                                                                             tcCTGCTGGAAGCCAGGCCGAAATGCCTTGCATGGGCACAGTATCGGCGCTCGATTGCCTACTGATAAGATGGTAATCACTATCAGCAGAGTAAACCTTTGGTTGCTAATCATGGCCATGGAATCATTCAGCGCGTTGGAATGCTTCAt                         >  1:32683/1‑149 (MQ=255)
                                                                                                               ctgctgGAAGCCAGGCCGAAATGCCTTGGATGGGCACAGTATCGGCGCTCGATTGCCTACTGATAAGATGGTAATCACTATCAGCAGAGTAAACCTTTGGTTGCTAATCATGGCCATGGAATCATTCAGCGCGTTGGAATGCTTCATcc                       >  2:164090/1‑149 (MQ=255)
                                                                                                               ctgctgGAAGCCAGGCCGAAATGCCTTGGATGGGCACAGTATCGGCGCTCGATTGCCTACTGATAAGATGGTAATCACTATCAGCAGAGTAAACCTTTGGTTGCTAATCATGGCCATGGAATCATTCAGCGCGTTGGAATGCTTCATc                        <  1:196436/148‑1 (MQ=255)
                                                                                                                     gAAGCCAGGCCGAAATGCCTTGGATGGGCACAGTATCGGCGCTCGATTGCCTACTGATAAGATGGTAATCACTATCAGCAGAGTAAACCTTTGGTTGCTAATCATGGCCATGGAATCATTCAGCGCGTTGGAATGCTTCATCCGCAGTg                 >  5:122695/1‑149 (MQ=255)
                                                                                                                          cAGGCCGAAATGCCTTGGATGGGCACAGTATCGGCGCTCGATTGCCTACTGATAAGATGGTAATCACTATCAGCAGAGTAAACCTTTGGTTGCTAATCATGGCCATGGAATCATTCAGCGCGTTGGAATGCTTCATCCGCAGTGCCGAg            <  3:138669/149‑1 (MQ=255)
                                                                                                                           aGGCCGAAATGCCTTGGATGGGCACAGTATCGGCGCTCGATTGCCTACTGATAAGATGGTAATCACTATCAGCagag                                                                                   >  5:195249/1‑77 (MQ=255)
                                                                                                                           aGGCCGAAATGCCTTGGATGGGCACAGTATCGGCGCTCGATTGCCTACTGATAAGATGGTAATCACTATCAGCagag                                                                                   <  6:195249/77‑1 (MQ=255)
                                                                                                                             gCCGAAATGCCTTGGATGGGCACAGTATCGGCGCTCGATTGCCTACTGATAAGATGGTAATCACTATCAGCAGAGTAAACCTTTGGTTGCTAATCATGGCCATGGAATCATTCAGCGCGTTGGAATGCTTCATc                        <  8:52524/134‑1 (MQ=255)
                                                                                                                             gCCGAAATGCCTTGGATGGGCACAGTATCGGCGCTCGATTGCCTACTGATAAGATGGTAATCACTATCAGCAGAGTAAACCTTTGGTTGCTAATCATGGCCATGGAATCATTCAGCGCGTTGGAATGCTTCATc                        >  7:52524/1‑134 (MQ=255)
                                                                                                                             gCCGAAATGCCTTGGATGGGCACAGTATCGGCGCTCGATTGCCTACTGATAAGATGGTAATCACTATCAGCAGAGTAAACCTTTGGTTGCTAATCATGGCCATGGAATCATTCAGCGCGTTGGAATGCTTCATCCGCAGTGCCGAGGTc         <  8:22697/149‑1 (MQ=255)
                                                                                                                               cGAAATGCCTTGGATGGGCACAGTATCGGCGCTCGATTGCCTACTGATAAGATGGTAATCACTATCAGCAGAGTAAACCTTTGGTTGCTAATCATGGCCATGGAATCATTCAGCGCGTTGGAATGCTTCATCCGCAGTGCCGAGGTCgg       <  1:164090/149‑1 (MQ=255)
                                                                                                                                    tGCCTTGGATGGGCACAGTATCGGCGCTCGATTGCCTACTGATAAGATGGTAATCACTATCAGCAGAGTAAACCTTTGGTTGCTAATCATGGCCATGGAATCATTCAGCGCGTTGGAATGCTTCATCCGCAGTGCCGAGGTCGGCAGtt  <  1:182657/149‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                             
CGCGCCTTCGCGGGCCAGGCGCCTTACGATAGCTGCGCCAATGCCTCGGGAACCGCCCTGAACCAGGGCCACTTTGCCTTCGAGTGTAAGTTGCTTGGACATGCTGTTCTCCTGCTGGAAGCCAGGCCGAAATGCCTTGGATGGGCACAGTATCGGCGCTCGATTGCCTACTGATAAGATGGTAATCACTATCAGCAGAGTAAACCTTTGGTTGCTAATCATGGCCATGGAATCATTCAGCGCGTTGGAATGCTTCATCCGCAGTGCCGAGGTCGGCAGTT  >  NC_002947/2077057‑2077337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑