Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,083,643 C→A 8.8% F99L (TTC→TTA) ‡ PP_1862 → membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,083,6430CA 8.8% 216.5 / 9.1 68F99L (TTC→TTA) ‡PP_1862membrane protein
Reads supporting (aligned to +/- strand):  ref base C (34/28);  new base A (2/4);  total (36/32)
Fisher's exact test for biased strand distribution p-value = 4.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01

ATGGTGGTGCCGAACGTGCTGCTGGCGCTGTGGCGGTATCACCAGCGCAACCGCATCGCGTTTCGCCATGCGTTGCCGCTGTCGGCGTGCAGCTTCTTGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTCGGCTTCCTGGTAGCGTTGGCGGTGTGGAACGTGGTGCGGATGTTCATGAAGGTGTCGCCGCCGAGTGCCGAGCTGCGTCATGCCTGGCCGTGGCTGGGTGTGCTGGGCAGCTTTGCCGGCACCATGGG  >  NC_002947/2083497‑2083789
                                                                                                                                                  |                                                                                                                                                  
aTGGTGGTGCCGAACGTGCTGCTGGCGCTGTGGCGGTATCACCAGCGCAACCGCATCGCGTTTCGCCATGCGTTGCCGCTGTCGGCGTGCAGCTTCTTGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCa                                                                                                                                                   <  2:329761/148‑1 (MQ=255)
   gtggtgCCGAACGTGCTGCTGGCGCTGTGGCGGTATCACCAGCGCAACCGCATCGCGTTTCGCCATGCGTTGCCGCTGTCGGCGTGCAGCTTCTTGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATgcg                                                                                                                                               >  3:208071/1‑149 (MQ=255)
   gtggtgCCGAACGTGCTGCTGGCGCTGTGGCGGTATCACCAGCGCAACCGCATCGCGTTTCGCCATGCGTTGCCGCTGTCGGCGTGCAGCTTCTTGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATgcg                                                                                                                                               >  3:9121/1‑149 (MQ=255)
      gtgCCGAACGTGCTGCTGGCGCTGTGGCGGTATCACCAGCGCAACCGCATCGCGTTTCGCCATGCGTTGCCGCTGTCGGCGTGCAGCTTCTTGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATGAAATgcgcat                                                                                                                                            <  2:10907/149‑3 (MQ=255)
       tgCCGAACGTGCTGCTGGCGCTGTGGCGGTATCACCAGCGCAACCGCATCGCGTTTCGCCATGCGTTGCCGCTGTCGGCGTGCAGCTTCTTGTTCGCCGGGCTGGGGTCGAGCTGGGCGGGGGGGCTTGATGCGCATGAAATgcgcatc                                                                                                                                           <  5:116019/149‑4 (MQ=255)
           gAACGTGCTGCTGGCGCTGTGGCGGTATCACCAGCGCAACCGCATCGCGTTTCGCCATGCGTTGCCGCTGTCGGCGTGCAGCTTCTTGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTggg                                                                                                                                         <  1:84997/147‑1 (MQ=255)
           gAACGTGCTGCTGGCGCTGTGGCGGTATCACCAGCGCAACCGCATCGCGTTTCGCCATGCGTTGCCGCTGTCGGCGTGCAGCTTCTTGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTggg                                                                                                                                         >  2:84997/1‑147 (MQ=255)
           gAACGTGCTGCTGGCGCTGTGGCGGTATCACCAGCGCAACCGCATCGCGTTTCGCCATGCGTTGCCGCTGTCGGCGTGCAGCTTCTTGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGtt                                                                                                                                       >  5:41027/1‑149 (MQ=255)
               gTGCTGCTGGCGCTGTGGCGGTATCACCAGCGCAACCGCATCGCGTTTCGCCATGCGTTGCCGCTGTCGGCGTGCAGCTTCTTGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATGAAATgcgca                                                                                                                                             <  7:328060/139‑2 (MQ=255)
               gTGCTGCTGGCGCTGTGGCGGTATCACCAGCGCAACCGCATCGCGTTTCGCCATGCGTTGCCGCTGTCGGCGTGCAGCTTCTTGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATGAAATgcgca                                                                                                                                             >  8:328060/1‑138 (MQ=255)
                tgctgcTGGCGCTGTGGCGGTATCACCAGCGCAACCGCATCGCGTTTCGCCATGCGTTGCCGCTGTCGGCGTGCAGCTTCTTGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTc                                                                                                                                  <  4:9121/149‑1 (MQ=255)
                 gctgctGGCGCTGTGGCGGTATCACCAGCGCAACCGCATCGCGTTTCGCCATGCGTTGCCGCTGTCGGCGTGCAGCTTCTTGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTgg                                                                                                                                          >  6:134758/1‑140 (MQ=255)
                 gctgctGGCGCTGTGGCGGTATCACCAGCGCAACCGCATCGCGTTTCGCCATGCGTTGCCGCTGTCGGCGTGCAGCTTCTTGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTgg                                                                                                                                          <  5:134758/140‑1 (MQ=255)
                      tgGCGCTGTGGCGGTATCACCAGCGCAACCGCATCGCGTTTCGCCATGCGTTGCCGCTGTCGGCGTGCAGCTTCTTGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTCGGCTTc                                                                                                                            >  1:212402/1‑149 (MQ=255)
                       ggCGCTGTGGCGGTATCACCAGCGCAACCGCATCGCGTTTCGCCATGCGTTGCCGCTGTCGGCGTGCAGCTTCTTGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCGTTTCATGCGCCTGGGTTTTGTCGGCTTcc                                                                                                                           >  1:230914/1‑149 (MQ=255)
                        gcgcTGTGGCGGTATCACCAGCGCAACCGCATCGCGTTTCGCCATGCGTTGCCGCTGTCGGCGTGCAGCTTCTTGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCCTTTCATGCGCCTGGGTTTTGTCGGCTTcc                                                                                                                           <  2:230914/148‑1 (MQ=255)
                        gcgcTGTGGCGGTATCACCAGCGCAACCGCATCGCGTTTCGCCATGCGTTGCCGCTGTCGGCGTGCAGCTTCTTGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATgcgc                                                                                                                                              >  4:297759/1‑129 (MQ=255)
                        gcgcTGTGGCGGTATCACCAGCGCAACCGCATCGCGTTTCGCCATGCGTTGCCGCTGTCGGCGTGCAGCTTCTTGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATgcgc                                                                                                                                              <  3:297759/129‑1 (MQ=255)
                        gcgcTGTGGCGGTATCACCAGCGCAACCGCATCGCGTTTCGCCATGCGTTGCCGCTGTCGGCGGGCAGCTTCTTGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTCGGCTTcc                                                                                                                           <  4:251808/148‑1 (MQ=255)
                         cgcTGTGGCGGTATCACCAGCGCAACCGCATCGCGTTTCGCCATGCGTTGCCGCTGTCGGCGTGCAGCTTCTTGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTCGGCTTCCTg                                                                                                                         <  4:20851/149‑1 (MQ=255)
                          gcTGTGGCGGTATCACCAGCGCAACCGCATCGCGTTTCGCCATGCGTTGCCGCTGTCGGCGTGCAGCTTCTTGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTCGGCTTCCTgg                                                                                                                        <  1:186597/149‑1 (MQ=255)
                            tgtgGCGGTATCACCAGCGCAACCGCATCGCGTTTCGCCATGCGTTGCCGCTGTCGGCGTGCAGCTTCTTGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTCGGCTTCCTgg                                                                                                                        >  3:354635/1‑147 (MQ=255)
                                    tATCACCAGCGCAACCGCATCGCGTTTCGCCATGCGTTGCCGCTGTCGGCGTGCAGCTTCTTGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTCGGCTTCCTGGTAGCGTTGGc                                                                                                              <  6:286614/149‑1 (MQ=255)
                                        aCCAGCGCAACCGCATCGCGTTTCGCCATGCGTTGCCGCTGTCGGCGTGCAGCTTCTTGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTCGGCTTCCTGGTAGCGTTGGCGgtg                                                                                                          >  5:246117/1‑149 (MQ=255)
                                        aCCAGCGCAACCGCATCGCGTTTCGCCATGCGTTGCCGCTGTCGGCGTGCAGCTTCTTGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTCGGCTTCCTGGTAGCGTTGGCGgtg                                                                                                          >  3:158700/1‑149 (MQ=255)
                                        aCCAGCGCAACCGCATCGCGTTTCGCCATGCGTTGCCGCTGTCGGCGTGCAGCTTCTTGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATGAAATgcgca                                                                                                                                             >  3:198540/1‑113 (MQ=255)
                                        aCCAGCGCAACCGCATCGCGTTTCGCCATGCGTTGCCGCTGTCGGCGTGCAGCTTCTTGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATGAAATgcgca                                                                                                                                             <  4:198540/114‑2 (MQ=255)
                                          cAGCGCAACCGCATCGCGTTTCGCCATGCGTTGCCGCTGTCGGCGTGCAGCTTCTTGTTCGCCTGGCTGGGGTCGATATGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTCGGCTGCATGGTAGCGTTGGCGGtgtg                                                                                                        >  7:270184/1‑149 (MQ=255)
                                               cAACCGCATCGCGTTTCGCCATGCGTTGCCGCTGTCGGCGTGCAGCTTCTTGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTc                                                                                                                                  >  3:19790/1‑118 (MQ=255)
                                               cAACCGCATCGCGTTTCGCCATGCGTTGCCGCTGTCGGCGTGCAGCTTCTTGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTc                                                                                                                                  <  4:19790/118‑1 (MQ=255)
                                                 aCCGCATCGCGTTTCGCCATGCGTTGCCGCTGTCGGCGTGCAGCTTCTTGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTCGGCTTCCTGGTAGCGTTGGCGGTGTGGAACgtg                                                                                                 <  6:246117/149‑1 (MQ=255)
                                                 aCCGCATCGCGTTTCGCCATGCGTTGCCGCTGTCGGCGTGCAGCTTCTTGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTCGGCTTCCTGGTAGCGTTGGCGGTGTGGAACgtg                                                                                                 <  1:282884/149‑1 (MQ=255)
                                                 aCCGCATCGCGTTTCGCCATGCGTTGCCGCTGTCGGCGTGCAGCTTCTTGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTCGGCTTCCTGGTAGCGTTGGCGGTGTGGAACgtg                                                                                                 <  2:338772/149‑1 (MQ=255)
                                                 aCCGCAGCGCGTTTCGCCATGCGTTGCCGCTGTCGGCGTGCAGCTTCTTGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTCGGCTTCCTGGTAGCGTTGGCGGTGTGGAACgtg                                                                                                 <  2:126166/149‑1 (MQ=255)
                                                      aTCGCGTTTCGCCATGCGTTGCCGCTGTCGGCGTGCAGCTTCTTGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTCGGCTTCCTGGTAGCGTTGGCGGTGTGGAACGTGGTGCg                                                                                            >  4:78634/1‑149 (MQ=255)
                                                         gcgTTTCGCCATGCGTTGCCGCTGTCGGCGTGCAGCTTCTTGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTCGGCTTCCTGGTAGCGTTGGCGGTGTGGAACGTGGTGCGGAt                                                                                         >  1:139964/1‑149 (MQ=255)
                                                           gTTTCGCCATGCGTTGCCGCTGTCGGCGTGCAGCTTCTTGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTCGGCTTCCTGGTAGCGTTGGCGGTGTGGAACGTGGTGCGGATGt                                                                                       >  5:283047/1‑149 (MQ=255)
                                                                  cATGCGTTGCCGCTGTCGGCGTGCAGCTTCTTGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTCGGCTTCCTGGTAGCGTTGGCGGTGTGGAACGTGGTGCGGATGTTCATg                                                                                  >  6:372018/1‑147 (MQ=255)
                                                                   aTGCGTTGCCGCTGTCGGCGTGCAGCTTCTTGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTCGGCTTCCTGGTAGCGTTGGCGGTGTGGAACGTGGTGCGGATGTTCATGAAg                                                                               <  6:283047/149‑1 (MQ=255)
                                                                      cGTTGCCGCTGTCGGCGTGCAGCTTCTTGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTCGGCTTCCTGGTAGCGTTGGCGGTGTGGAACGTGGTGCGGATGTTCATGAAGgt                                                                             <  2:212402/148‑1 (MQ=255)
                                                                           ccGCTGTCGGCGTGCAGCTTCTTGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTCGGCTTCCTGGTAGCGTTGGCGGTGTGGAACGTGGTGCGGATGTTCATGAAGGTGTcgcc                                                                       >  8:270879/1‑149 (MQ=255)
                                                                             gCTGTCGGCGTGCAGCTTCTTGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTCGGCTTCCTGGTAGCGTTGGCGGTGTGGAACGTGGTGCGGATGTTCATGAAGGTGTcgccgc                                                                     >  2:55232/1‑149 (MQ=255)
                                                                              cTGTCGGCGTGCAGCTTCTTGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCGTGATGCGCATTTCATGCGCCTGGGTTTTGTCGGCTTCCTGGTAGCGTTGGCGGTGTGGAACGTGGTGCGGATGTTCATGAAGGTGTCgccgcc                                                                    <  6:153466/149‑1 (MQ=255)
                                                                                 tCGGCGTGCAGCTTCTTGTTCGCCTGGCTGGGGGCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTCGGCTTCCTGGTAGCGTTGGCGGTGTGGAACGTGGTGCGGATGTTCATGAAGGTGTCGCCGCCGAg                                                                 <  7:292631/149‑1 (MQ=255)
                                                                                       tGCAGCTTCTTGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTCGGCTTCCTGGTAGCGTTGGCGGTGTGGAACGTGGTGCGGATGTTCATGAAGgtg                                                                            >  4:7520/1‑132 (MQ=255)
                                                                                       tGCAGCTTCTTGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTCGGCTTCCTGGTAGCGTTGGCGGTGTGGAACGTGGTGCGGATGTTCATGAAGgtg                                                                            <  3:7520/132‑1 (MQ=255)
                                                                                        gCAGCTTCTTGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTCGGCTTCCTGGTAGCGTTGGCGGTGTGGAACGTGGTGCGGATGTTCATGAAGGTGTCGCCGCCGAGTGCCGAg                                                          >  6:306557/1‑149 (MQ=255)
                                                                                              tcttGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTCGGCTTCCTGGTAGCGTTGGCGGTGTGGAACGTGGTGCGGATGTTCATGAAGGTGTCGCCGCCGAGTGCCGAGCTGCg                                                     >  3:354485/1‑148 (MQ=255)
                                                                                                  gTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTCGGCTTCCTGGTAGCGTTGGCGGTGTGGAACGTGGTGCGGATGTTCATGAAGGTGTCGCCGCCGAGTGCCGAGCTGcccagca                                                >  3:353858/1‑143 (MQ=255)
                                                                                                  gTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTCGGCTTCCTGGTAGCGTTGGCGGTGTGGAACGTGGTGCGGATGTTCATGAAGGTGTCGCCGCCGAGTGCCGAGCTGCGTc                                                   <  2:139964/146‑1 (MQ=255)
                                                                                                   ttCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTCGGCTTCCTGGTAGCGTTGGCGGTGTGGAACGTGGTGCGGATGTTCATGAAGGTGTCGCCGCCGAGTGCCGAGCTGCGTCATg                                                <  4:354485/148‑1 (MQ=255)
                                                                                                       ccTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTCGGCTTCCTGGTAGCGTTGGCGGTGTGGAACGTGGTGCGGATGTTCATGAAg                                                                               <  8:107845/113‑1 (MQ=255)
                                                                                                       ccTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTCGGCTTCCTGGTAGCGTTGGCGGTGTGGAACGTGGTGCGGATGTTCATGAAg                                                                               >  7:107845/1‑113 (MQ=255)
                                                                                                              ggggTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTCGGCTTCCTGGTAGCGTTGGCGGTATGGAACGTGGTGCGGATGTTCATGAAGGTGTCGCCGCCGAGTGCCGAGCTGCGTCATGCCTGGCCGTGGc                                    >  7:286101/1‑149 (MQ=255)
                                                                                                               gggTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTCGGCTTCCTGGTAGCGTTGGCGGTGTGGAACGTGGTGCGGATGTTCATGAAGGTGTCGCCGCCGAGTGCCGAGCTGCGTCATGCCTGGCCGTGGCt                                   >  7:350279/1‑149 (MQ=255)
                                                                                                                 gTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTCGGCTTCCTGGTAGCGTTGGCGGTGTGGAACGTGGTGCGGATGTTCATGAAGGTGTCGCCGCCGAGTGCCGAGCTGCGTCATGCCTGGCCGTGGCTgg                                 <  3:78634/149‑1 (MQ=255)
                                                                                                                 gTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTCGGCTTCCTGGTAGCGTTGGCGGTGTGGAACGTGGTGCGGATGTTCATGAAGGTGTCGCCGCCGAGTGCCGAGCTGCGTCATGCCTGGCCGTGGCTgg                                 <  4:208071/149‑1 (MQ=255)
                                                                                                                 gTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTCGGCTTCCTGGTAGCGTTGGCGGTGTGGAACGTGGTGCGGATGTTCATGAAGGTGTCGCCGCCGAGTGCCGAGCTGCGTCATGCCTGGCCGTGGCTgg                                 <  4:158700/149‑1 (MQ=255)
                                                                                                                 gTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTCGGCTTCCTGGTAGCGTTGGCGGTATGGAACGTGGTGCGGATGTTCATGAAGGTGTCGCCGCCGAGTGCCGAGCTGCGTCATGCCTGGCCGTGGCTgg                                 <  8:286101/149‑1 (MQ=255)
                                                                                                                  tCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTCGGCTTCCTGGTAGCGTTGGCGGTGTGGAACGTGGTGCGGATGTTCATGa                                                                                 <  7:12249/100‑1 (MQ=255)
                                                                                                                  tCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTCGGCTTCCTGGTAGCGTTGGCGGTGTGGAACGTGGTGCGGATGTTCATGa                                                                                 >  8:12249/1‑100 (MQ=255)
                                                                                                                    gATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTCGGCTTCCTGGTAGCGTTGGCGGTGTGGAACGTGGTGCGGATGTTCATGAAGGTGTCGCCGCCGAGTGCCGAGCTGCGTCATGCCTGGCCGTGGCTGGgtg                              >  7:204777/1‑149 (MQ=255)
                                                                                                                    gATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTCGGCTTCCTGGTAGCGTTGGCGGTGTGGAACGTGGTGCGGATGTTCATGAAGGTGTCGCCGCCGAGTGCCGAGCTGCGTCATGCCTGGCCGTGGCTGGgtg                              >  1:249547/1‑149 (MQ=255)
                                                                                                                          ggCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTCGGCTTCCTGGTAGCGTTGGCGGTGTGGAACGTGGTGCGGATGTTCATGAAGGTGTCGCCGCCGAGTGCCGAGCTGCGTCATGCCTGGCCGTGGCTGGGTGTGCTgg                        >  3:204765/1‑149 (MQ=255)
                                                                                                                                    cTTGATGCGCATTTCATGCGCCTGGGTTTTGTCGGCTTCCTGGTAGCGTTGGCGGTGTGGAACGTGGTGCGGATGTTCATGAAGGTGTCGCCGCCGAGTGCCGAGCTGCGTCATGCCTGGCCGTGGCTGGGTGTGCTGGGCAGCTTTGc              >  3:300384/1‑149 (MQ=255)
                                                                                                                                          gcgcATTTCATGCGCCTGGGTTTTGTCGGCTTCCTGGTAGCGTTGGCGGTGTGGAACGTGGTGCGGATGTTCATGAAGGTGTCGCCGCCGAGTGCCGAGCTGCGTCATGCCTGGCCGTGGCTGGGTGTGCTGGGCAGCTTTGCCGGCAc        >  7:148217/1‑149 (MQ=255)
                                                                                                                                            gcATTTCATGCGCCTGGGTTTTGTCGGCTTCCTGGTAGCGTTGGCGGTGTGGAACGTGGTGCGGATGTTCATGAAGGTGTCGCCGCCGAGTGCCGAGCTGCGTCATGCCTGGCCGTGGCTGGGTGTGCTGGGCAGCTTTGCCGGCACCa      >  8:90677/1‑149 (MQ=255)
                                                                                                                                                ttCATGCGCCTGGGTTTTGTCGGCTTCCTGGTAGCGTTGGCGGTGTGGAACGTGGTGCGGATGTTCATGAAGGTGTCGCCGCCGAGTGCCGAGCTGCGTCATGCCTGGCCGTGGCTGGGTGTGCTGGGCAGCTTTGCCGGCACCATggg  >  1:209268/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
ATGGTGGTGCCGAACGTGCTGCTGGCGCTGTGGCGGTATCACCAGCGCAACCGCATCGCGTTTCGCCATGCGTTGCCGCTGTCGGCGTGCAGCTTCTTGTTCGCCTGGCTGGGGTCGATCTGGGCGGTGGGGCTTGATGCGCATTTCATGCGCCTGGGTTTTGTCGGCTTCCTGGTAGCGTTGGCGGTGTGGAACGTGGTGCGGATGTTCATGAAGGTGTCGCCGCCGAGTGCCGAGCTGCGTCATGCCTGGCCGTGGCTGGGTGTGCTGGGCAGCTTTGCCGGCACCATGGG  >  NC_002947/2083497‑2083789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑