| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,087,373 | T→C | 7.3% | intergenic (+186/+732) | PP_1865 → / ← aroH | transposase/3‑deoxy‑7‑phosphoheptulonate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,087,373 | 0 | T | C | 7.3% | 157.5 / 2.5 | 56 | intergenic (+186/+732) | PP_1865/aroH | transposase/3‑deoxy‑7‑phosphoheptulonate synthase |
| Reads supporting (aligned to +/- strand): ref base T (27/24); new base C (2/2); total (30/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.52e-01 | |||||||||||
GGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCC‑ATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGCCCGGT > NC_002947/2087232‑2087520 | ggAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCC‑ATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATctccctc > 5:161755/1‑149 (MQ=255) gCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCC‑ATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCg < 5:71880/149‑1 (MQ=255) gCTCCCATACTCCGGCCCCTACGCTGCGGTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGATCA‑AGGGGTGCATGCCCCAACCTATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTc < 1:110861/149‑1 (MQ=17) atcatcCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTTCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCC‑ATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTGATCTACCTCCCCAATGCCTCCACACGGcctcc > 2:293603/1‑149 (MQ=17) ccTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCC‑ATGGGGCCGCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGc > 3:101028/1‑123 (MQ=255) ccTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCC‑ATGGGGCCCCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGc < 4:101028/123‑1 (MQ=255) cGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCC‑ATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCt < 8:197688/148‑1 (MQ=255) cGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCC‑ATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTg < 1:153461/148‑1 (MQ=255) cGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCACGCCCCATAC‑AAGGGGCCTCAGCCCTTGACGGGCATCCATGCCCGTCATCACCCAACGCAATGCCTCCACTCGGCCTCCTCAAGTCGCAATCTGCGTCGCCTg > 4:86807/1‑148 (MQ=17) ggggTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCAACC‑ATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCAACTCCCACCGCAATACCTCCACTCGGCCTCCAGAAGTCGCAATCAGCGTCGCCTggc > 4:272272/1‑148 (MQ=14) cccTCACTCCGGGCTTGCTCCCGGGAGGACCGCGCTGCAGGCCCCATCC‑TTGGGGCCTCAGCGCTTGACGTGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGt < 5:226453/136‑1 (MQ=11) ccTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCC‑ATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACcgc > 1:191662/1‑149 (MQ=255) tCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCC‑ATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACcgcgc > 2:80880/1‑149 (MQ=255) tCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCC‑ATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCt > 8:174113/1‑148 (MQ=255) cGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCC‑ATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCt > 3:256089/1‑146 (MQ=255) cTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCC‑ATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAg > 5:335850/1‑148 (MQ=255) cgcGGGAGCA‑CGCGCTGGAGCCCCCATCC‑ATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGAGTACCGCGCGCTTAGAAGAAAGATc < 2:245398/147‑1 (MQ=255) cccGCGAGCA‑CGCGCTGCAGGCCCCAACC‑ATTCGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAAAGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCGGGGTCTACCGCGCGCGTAGAAGCAAGATc < 2:245395/149‑1 (MQ=17) ccGGGAGCA‑CGCGCTGCAGGCCCCATCC‑ATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATc < 3:272272/148‑1 (MQ=255) ggAGCA‑CGCGCTGCAGGCCCCATCC‑ATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCaaaa < 7:174113/149‑1 (MQ=255) gCA‑CGGGCTGCAGGCGCCATCC‑ATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATGTCGCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCg < 7:312987/149‑1 (MQ=255) a‑CGCGCTGCAGGCCCCATCC‑ATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGAt > 6:96144/1‑139 (MQ=255) a‑CGCGCTGCAGGCCCCATCC‑ATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGAt < 5:96144/139‑1 (MQ=255) ccgcgCTGCAGGCCCCATCC‑‑TGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGa < 1:191656/112‑1 (MQ=1) ccgcgCTGCAGGCCCCATCC‑‑TGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCACCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGa > 2:191656/2‑113 (MQ=1) aGGCCCCATCC‑ATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAAtt < 3:49770/149‑1 (MQ=255) ggCCCCATCC‑ATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGATTACCGCGCGCTTAGAAGc > 3:63916/1‑124 (MQ=255) ggCCCCATCC‑ATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGATTACCGCGCGCTTAGAAGc < 4:63916/124‑1 (MQ=255) ccccATGG‑ATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCgg > 4:146469/1‑149 (MQ=255) ccccATCC‑ATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCgg > 1:224137/1‑149 (MQ=255) cccATCC‑ATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGt > 4:291671/1‑149 (MQ=255) cccATCC‑‑TGGGGCC‑CAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGa > 1:61462/1‑149 (MQ=255) ccatcc‑atGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGt > 2:225541/1‑148 (MQ=255) atca‑atGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTCGACTACCGCGCGCGTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTCAt > 6:34518/1‑149 (MQ=255) gCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCa > 8:195084/1‑149 (MQ=255) cTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCt < 7:240146/85‑1 (MQ=255) cTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCt > 8:240146/1‑85 (MQ=255) cAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCa > 5:172470/1‑145 (MQ=255) cAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCa < 6:172470/145‑1 (MQ=255) aGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATcggccg > 3:171549/1‑141 (MQ=255) aGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATcggccg < 4:171549/141‑1 (MQ=255) cgcTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGa < 6:161755/149‑1 (MQ=255) gcTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGAt < 1:293603/149‑1 (MQ=255) cTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATg < 4:256089/149‑1 (MQ=255) gACGGGCATCCATGCCCGTCATCTCCATCCGCAATGCCTCCAATAGGCCTCCTGAAGTCTCAATCTGCGTCGCCTGGACTACCGCGCGCTTACAAGCAAGATCAAAAGCGAGAAGCGAAATCGTTGATCGGCCGGCACCGCCGATGCa > 7:150117/1‑148 (MQ=25) gCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGc > 3:187091/1‑149 (MQ=255) ccATGCCCGTCACCTCCCTCCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAgg < 2:166238/149‑1 (MQ=18) aTGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGa < 7:167705/145‑1 (MQ=255) aTGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGa > 8:167705/1‑145 (MQ=255) cccGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCt < 1:80880/148‑1 (MQ=255) ccGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGc > 3:158198/1‑149 (MQ=255) ccGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGc > 2:28105/1‑149 (MQ=255) ccGTCACCTCCCTCCGCAAGCCCTGCGCTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGc > 1:348206/1‑149 (MQ=21) cGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTTGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGATGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGcc < 5:34518/149‑1 (MQ=255) cGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGAAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGcc > 4:25447/1‑149 (MQ=255) aTCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGCCCGGt < 4:158198/149‑1 (MQ=255) | GGAACCAAAAACCGTCGGCTCCCATCATCCGGCCCCTACGCTGCGCTCCGGGGTCCCCTCGCTCCGGCCTTGCTCCCGGGAGCA‑CGCGCTGCAGGCCCCATCC‑ATGGGGCCTCAGCGCTTGACGGGCATCCATGCCCGTCATCTCCCTCCGCAATGCCTCCACTCGGCCTCCTGAAGTCGCAATCTGCGTCGCCTGGACTACCGCGCGCTTAGAAGCAAGATCAAAAGCGAGAAGCGAATTCGGTGATCGGCCGGCACCGCCGATGCATTCGGCGAGGTTCTGCCCGGT > NC_002947/2087232‑2087520 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |